LOCUS       pLenti6(puro)-FLAG-dest-strep-hMLKL(1-95)-stop      7537 bp    DNA     circular SYN 09-FEB-2012
DEFINITION  LMBP 8831.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES             Location/Qualifiers
     Misc._recombina join(7531..7537,1..14)
                     /label=attB1
     Source          join(<386..>408,<1..>56)
                     /label=Source_1
     Vector          join(386..>408,<1..56)
                     /origin="pENTR3C (2.723bp circular)          Target
                     vector: from XhoI cut at 938^939 to EcoRI cut at 496^497,
                     2.281bp          Orientation: original          Plus
                     strand 5':   no change          Plus strand 3':   no
                     change          Minus strand 5':  no change          Minus
                     strand 3':  no change"
     Misc._signal    65..100
                     /label="Strep Tag"
     CDS             101..382
                     /origin="strep + hMLKL(1-125) fragment + primers (445bp
                     linear)           Fragment: from EcoRI cut at 11^12 to
                     XhoI cut at 430^431, 419bp           Orientation: original
                     Plus strand 5':   no change           Plus strand 3':   no
                     change           Minus strand 5':  no change
                     Minus strand 3':  no change"
                     /label="hMLKL (1 - 95) - stop"
     Misc._recombina complement(402..422)
                     /label=attB2
     Misc._feature   466..490
                     /label="FLAG tag"
     CDS             503..511
                     /label="3 stops"
     Promoter        548..857
                     /label="SV40 early promoter"
     CDS             917..1519
                     /label=Puro(R)
     LTR             1610..1663
                     /label=deltaU3
     LTR             1611..1844
                     /label="deltaU3/3' LTR"
     LTR             1664..1844
                     /label="HIV-1 3' LTR"
     PolyA_signal    1916..2046
                     /label="SV40 pA"
     Replication_ori 2419..2874
                     /label="f1 origin"
     Promoter        2906..3004
                     /label="bla promoter"
     CDS             3005..3865
                     /label=Amp(R)
     Replication_ori 4010..4683
                     /label="pUC origin"
     Promoter        5091..5319
                     /label="RSV promoter"
     Promoter        5091..5500
                     /label="RSV/5' LTR hybrid promoter"
     Source          5090^5091
                     /label=Source_1
     LTR             5320..5500
                     /label="HIV-1 5' LTR"
     Misc._feature   5610
                     /label="splice donor"
     Misc._binding   5611..5655
                     /label="HIV-1 psi packaging signal"
     Protein_binding 6165..6398
                     /label="HIV-1 Rev response element"
     Misc._feature   6746
                     /label="splice acceptor"
     Misc._feature   6774
                     /label="splice acceptor"
     Promoter        6899..7481
                     /label="CMV promoter"
     TATA_box        7399..7402
                     /label="TATA box"
ORIGIN
        1 GTACAAAAAA GCAGGCTCTT TAAAGGAACC AATTCAGTCG ACTGGATCCG GTACCGAATT
       61 CACCATGGCT AGCTGGAGCC ACCCGCAGTT CGAAAAAGCC GAAAATTTGA AGCATATTAT
      121 CACCCTTGGC CAGGTCATCC ACAAACGGTG TGAAGAGATG AAATACTGCA AGAAACAGTG
      181 CCGGCGCCTG GGCCACCGCG TCCTCGGCCT GATCAAGCCT CTGGAGATGC TCCAGGACCA
      241 AGGAAAGAGG AGCGTGCCCT CTGAGAAGTT AACCACAGCC ATGAACCGCT TCAAGGCTGC
      301 CCTGGAGGAG GCTAATGGGG AGATAGAAAA GTTCAGCAAT AGATCCAATA TCTGCAGGTT
      361 TCTAACAGCA AGCCAGGACA AACACTCGAG ATATCTAGAC CCAGCTTTCT TGTACAAAGT
      421 GGTTGATATC CAGCACAGTG GCGGCCGCTC GAGTCTAGAG GGCCCGGACT ACAAGGATGA
      481 CGATGACAAA ACGCGTACCG GTTAGTAATG AGTTTGGAAT TAATTCTGTG GAATGTGTGT
      541 CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGGCAGGC AGAAGTATGC AAAGCATGCA
      601 TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT
      661 GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC
      721 GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT
      781 TTATGCAGAG GCCGAGGCCG CCTCTGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT
      841 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC
      901 TGATCAGCAC GTGACCATGG CCACCGAGTA CAAGCCCACG GTGCGCCTCG CCACCCGCGA
      961 CGACGTCCCC CGGGCCGTAC GCACCCTCGC CGCCGCGTTC GCCGACTACC CCGCCACGCG
     1021 CCACACCGTC GACCCGGACC GCCACATCGA GCGGGTCACC GAGCTGCAAG AACTCTTCCT
     1081 CACGCGCGTC GGGCTCGACA TCGGCAAGGT GTGGGTCGCG GACGACGGCG CCGCGGTGGC
     1141 GGTCTGGACC ACGCCGGAGA GCGTCGAAGC GGGGGCGGTG TTCGCCGAGA TCGGCTCGCG
     1201 CATGGCCGAG TTGAGCGGTT CCCGGCTGGC CGCGCAGCAA CAGATGGAAG GCCTCCTGGC
     1261 GCCGCACCGG CCCAAGGAGC CCGCGTGGTT CCTGGCCACC GTCGGCGTCT CGCCCGACCA
     1321 CCAGGGCAAG GGTCTGGGCA GCGCCGTCGT GCTCCCCGGA GTGGAGGCGG CCGAGCGCGC
     1381 TGGGGTGCCC GCCTTCCTGG AGACCTCCGC GCCCCGCAAC CTCCCCTTCT ACGAGCGGCT
     1441 CGGCTTCACC GTCACCGCCG ACGTCGAGGT GCCCGAAGGA CCGCGCACCT GGTGCATGAC
     1501 CCGCAAGCCC GGTGCCTGAG CTAAGCACAA TTCGAGCTCG GTACCTTTAA GACCAATGAC
     1561 TTACAAGGCA GCTGTAGATC TTAGCCACTT TTTAAAAGAA AAGGGGGGAC TGGAAGGGCT
     1621 AATTCACTCC CAACGAAGAC AAGATCTGCT TTTTGCTTGT ACTGGGTCTC TCTGGTTAGA
     1681 CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA
     1741 AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA
     1801 GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTAGTA GTTCATGTCA
     1861 TCTTATTATT CAGTATTTAT AACTTGCAAA GAAATGAATA TCAGAGAGTG AGAGGAACTT
     1921 GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA
     1981 AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA
     2041 TGTCTGGCTC TAGCTATCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT
     2101 TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC
     2161 GCCTCGGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGGACG
     2221 TACCCAATTC GCCCTATAGT GAGTCGTATT ACGCGCGCTC ACTGGCCGTC GTTTTACAAC
     2281 GTCGTGACTG GGAAAACCCT GGCGTTACCC AACTTAATCG CCTTGCAGCA CATCCCCCTT
     2341 TCGCCAGCTG GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA
     2401 GCCTGAATGG CGAATGGGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG
     2461 TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT
     2521 TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC
     2581 CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG
     2641 ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT
     2701 CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG
     2761 TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC
     2821 TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT AACGCTTACA ATTTAGGTGG
     2881 CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA
     2941 TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA
     3001 GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT
     3061 TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG
     3121 TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG
     3181 CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT
     3241 ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA
     3301 CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA
     3361 ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC
     3421 GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG
     3481 CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC
     3541 GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT
     3601 AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT
     3661 GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG
     3721 GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT
     3781 CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG
     3841 TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT
     3901 TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT
     3961 CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA
     4021 GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA
     4081 AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC
     4141 GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTTCTTCTAG TGTAGCCGTA
     4201 GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT
     4261 GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG
     4321 ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG
     4381 CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC
     4441 CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG
     4501 AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT
     4561 TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG
     4621 GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA
     4681 CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG
     4741 AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC
     4801 GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG
     4861 CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG
     4921 TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG
     4981 TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA
     5041 GCGCGCAATT AACCCTCACT AAAGGGAACA AAAGCTGGAG CTGCAAGCTT AATGTAGTCT
     5101 TATGCAATAC TCTTGTAGTC TTGCAACATG GTAACGATGA GTTAGCAACA TGCCTTACAA
     5161 GGAGAGAAAA AGCACCGTGC ATGCCGATTG GTGGAAGTAA GGTGGTACGA TCGTGCCTTA
     5221 TTAGGAAGGC AACAGACGGG TCTGACATGG ATTGGACGAA CCACTGAATT GCCGCATTGC
     5281 AGAGATATTG TATTTAAGTG CCTAGCTCGA TACATAAACG GGTCTCTCTG GTTAGACCAG
     5341 ATCTGAGCCT GGGAGCTCTC TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC
     5401 TTGCCTTGAG TGCTTCAAGT AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA
     5461 TCCCTCAGAC CCTTTTAGTC AGTGTGGAAA ATCTCTAGCA GTGGCGCCCG AACAGGGACT
     5521 TGAAAGCGAA AGGGAAACCA GAGGAGCTCT CTCGACGCAG GACTCGGCTT GCTGAAGCGC
     5581 GCACGGCAAG AGGCGAGGGG CGGCGACTGG TGAGTACGCC AAAAATTTTG ACTAGCGGAG
     5641 GCTAGAAGGA GAGAGATGGG TGCGAGAGCG TCAGTATTAA GCGGGGGAGA ATTAGATCGC
     5701 GATGGGAAAA AATTCGGTTA AGGCCAGGGG GAAAGAAAAA ATATAAATTA AAACATATAG
     5761 TATGGGCAAG CAGGGAGCTA GAACGATTCG CAGTTAATCC TGGCCTGTTA GAAACATCAG
     5821 AAGGCTGTAG ACAAATACTG GGACAGCTAC AACCATCCCT TCAGACAGGA TCAGAAGAAC
     5881 TTAGATCATT ATATAATACA GTAGCAACCC TCTATTGTGT GCATCAAAGG ATAGAGATAA
     5941 AAGACACCAA GGAAGCTTTA GACAAGATAG AGGAAGAGCA AAACAAAAGT AAGACCACCG
     6001 CACAGCAAGC GGCCGCTGAT CTTCAGACCT GGAGGAGGAG ATATGAGGGA CAATTGGAGA
     6061 AGTGAATTAT ATAAATATAA AGTAGTAAAA ATTGAACCAT TAGGAGTAGC ACCCACCAAG
     6121 GCAAAGAGAA GAGTGGTGCA GAGAGAAAAA AGAGCAGTGG GAATAGGAGC TTTGTTCCTT
     6181 GGGTTCTTGG GAGCAGCAGG AAGCACTATG GGCGCAGCGT CAATGACGCT GACGGTACAG
     6241 GCCAGACAAT TATTGTCTGG TATAGTGCAG CAGCAGAACA ATTTGCTGAG GGCTATTGAG
     6301 GCGCAACAGC ATCTGTTGCA ACTCACAGTC TGGGGCATCA AGCAGCTCCA GGCAAGAATC
     6361 CTGGCTGTGG AAAGATACCT AAAGGATCAA CAGCTCCTGG GGATTTGGGG TTGCTCTGGA
     6421 AAACTCATTT GCACCACTGC TGTGCCTTGG AATGCTAGTT GGAGTAATAA ATCTCTGGAA
     6481 CAGATTTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA TTACACAAGC
     6541 TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA ACAAGAATTA
     6601 TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA TTGGCTGTGG
     6661 TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT AGTTTTTGCT
     6721 GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT TCAGACCCAC
     6781 CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG TGGAGAGAGA
     6841 GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGATA AGCTTGGGAG
     6901 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC
     6961 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA
     7021 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT
     7081 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC
     7141 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT
     7201 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA
     7261 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT
     7321 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG
     7381 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCGTTTAG TGAACCGTCA GATCGCCTGG
     7441 AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGACACC GACTCTAGAG GATCCACTAG
     7501 TCCAGTGTGG TGGAATTCTG CAGATATCAA CAAGTTT
//
