LOCUS       pCAGGS-E-hA20-S9        7278 bp    DNA     circular      3-SEP-2019
DEFINITION  LMBP 8834.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 8834
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 03-SEP-2019
            
            
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12975192|
COMMENT     VNTDBDATE|-12975192|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCAGGS-E-hA20-S9A|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTREPLTYPE|Virus
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTEXTCHREPL|Animal/Other Eukaryotic
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-E-hA20-S9A" 0 7278 
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COMMENT        (CWordArray) (CStringList "BamHI" "EcoRI" "NotI" "PvuI" "SacI" "XhoI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
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COMMENT       (CMolPar 0 0 0 0 4294967295 1 7278 0 0 0 0 0 0 0 0) (CStringList) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT        #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 3 1612 0 3218 0 3555 0 0 
COMMENT            "") #1=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 4 0 2488 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 57 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 1 4999 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 2259 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 2476 0 0 "")) 
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COMMENT        #7=(CFSignal (CObList) "ampicillin resistance" 4 0 1 4555 5415 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(4555..5415)") 
COMMENT        #8=(CFSignal (CObList) "pMB1 ori" 33 0 1 3613 4014 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(3613..4014)") 
COMMENT        #9=(CFSignal (CObList) "SV40 polyA late" 43 0 1 3424 3498 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(3424..3498)") 
COMMENT        #10=(CFSignal (CObList) "SV40 polyA early" 43 0 0 3424 3498 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3424..3498") 
COMMENT        #11=(CFSignal (CObList) "SV40 ori" 33 0 0 3318 3393 0 (CStringList) 
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COMMENT        #13=(CFSignal (CObList) "3' UTR of rabbit \337-globin, incl. polyA" 43 0 
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COMMENT        #17=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 5551 5928 0 
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COMMENT        #18=(CFSignal (CObList) "3' flanking region of rabbit \337-globin" 21 0 
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COMMENT        #19=(CFSignal (CObList) "3' UTR of hA20" 21 0 0 2437 2480 0 
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COMMENT        #20=(CFSignal (CObList) "S548A" 62 0 0 1705 1707 0 (CStringList) 
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COMMENT        #22=(CFSignal (CObList) "S550A" 62 0 0 1711 1713 0 (CStringList) 
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COMMENT        #23=(CFSignal (CObList) "S552A" 62 0 0 1717 1719 0 (CStringList) 
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COMMENT        #24=(CFSignal (CObList) "S554A" 62 0 0 1723 1725 0 (CStringList) 
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COMMENT        #25=(CFSignal (CObList) "S558A" 62 0 0 1735 1737 0 (CStringList) 
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COMMENT        #26=(CFSignal (CObList) "S563A" 62 0 0 1750 1752 0 (CStringList) 
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COMMENT                          "DNA Plasmid Virus 'pCAGGS-E-hA20-S9A'" 1) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 
COMMENT                          1) 
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COMMENT                          "Created: 09/03/19 12:27" 1) 
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COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 7278 bp" 1) 
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COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.genecorner@UGent.be</a>" 
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FEATURES             Location/Qualifiers
     intron          6303..7227
                     /vntifkey="15"
                     /label=intron\AG\promoter
     CDS             complement(4555..5415)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(3613..4014)
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      complement(3424..3498)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     terminator      3424..3498
                     /vntifkey="43"
                     /label=SV40\polyA\early
     rep_origin      3318..3393
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        complement(3094..3176)
                     /vntifkey="30"
                     /label=lac\promoter
     terminator      2568..2659
                     /vntifkey="43"
                     /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA
     CDS             64..2436
                     /vntifkey="4"
                     /label=hA20
     misc_marker     16..54
                     /vntifkey="22"
                     /label=E-tag
     promoter        5934..7275
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     promoter        5551..5928
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
     misc_feature    2660..3016
                     /vntifkey="21"
                     /label=3'\flanking\region\of\rabbit\ß-globin
     misc_feature    2437..2480
                     /vntifkey="21"
                     /label=3'\UTR\of\hA20
     mutation        1705..1707
                     /vntifkey="62"
                     /label=S548A
                     /note="WT: TCC"
     mutation        1708..1710
                     /vntifkey="62"
                     /label=S549A
                     /note="WT: TCC"
     mutation        1711..1713
                     /vntifkey="62"
                     /label=S550A
                     /note="WT: AGC"
     mutation        1717..1719
                     /vntifkey="62"
                     /label=S552A
                     /note="WT: 552"
     mutation        1723..1725
                     /vntifkey="62"
                     /label=S554A
                     /note="WT: AGC"
     mutation        1735..1737
                     /vntifkey="62"
                     /label=S558A
                     /note="WT: TCC"
     mutation        1750..1752
                     /vntifkey="62"
                     /label=S563A
                     /note="WT: TCC"
     mutation        1756..1758
                     /vntifkey="62"
                     /label=S565A
                     /note="WT: TCA"
     mutation        1204..1206
                     /vntifkey="62"
                     /label=S381A
                     /note="WT: TCT"
BASE COUNT     1659 a      1954 c      1968 g      1697 t 
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 
       61 gcaatggctg aacaagtcct tcctcaggct ttgtatttga gcaatatgcg gaaagctgtg 
      121 aagatacggg agagaactcc agaagacatt tttaaaccta ctaatgggat cattcatcat 
      181 tttaaaacca tgcaccgata cacactggaa atgttcagaa cttgccagtt ttgtcctcag 
      241 tttcgggaga tcatccacaa agccctcatc gacagaaaca tccaggccac cctggaaagc 
      301 cagaagaaac tcaactggtg tcgagaagtc cggaagcttg tggcgctgaa aacgaacggt 
      361 gacggcaatt gcctcatgca tgccacttct cagtacatgt ggggcgttca ggacacagac 
      421 ttggtactga ggaaggcgct gttcagcacg ctcaaggaaa cagacacacg caactttaaa 
      481 ttccgctggc aactggagtc tctcaaatct caggaatttg ttgaaacggg gctttgctat 
      541 gatactcgga actggaatga tgaatgggac aatcttatca aaatggcttc cacagacaca 
      601 cccatggccc gaagtggact tcagtacaac tcactggaag aaatacacat atttgtcctt 
      661 tgcaacatcc tcagaaggcc aatcattgtc atttcagaca aaatgctaag aagtttggaa 
      721 tcaggttcca atttcgcccc tttgaaagtg ggtggaattt acttgcctct ccactggcct 
      781 gcccaggaat gctacagata ccccattgtt ctcggctatg acagccatca ttttgtaccc 
      841 ttggtgaccc tgaaggacag tgggcctgaa atccgagctg ttccacttgt taacagagac 
      901 cggggaagat ttgaagactt aaaagttcac tttttgacag atcctgaaaa tgagatgaag 
      961 gagaagctct taaaagagta cttaatggtg atagaaatcc ccgtccaagg ctgggaccat 
     1021 ggcacaactc atctcatcaa tgccgcaaag ttggatgaag ctaacttacc aaaagaaatc 
     1081 aatctggtag atgattactt tgaacttgtt cagcatgagt acaagaaatg gcaggaaaac 
     1141 agcgagcagg ggaggagaga ggggcacgcc cagaatccca tggaaccttc cgtgccccag 
     1201 cttgctctca tggatgtaaa atgtgaaacg cccaactgcc ccttcttcat gtctgtgaac 
     1261 acccagcctt tatgccatga gtgctcagag aggcggcaaa agaatcaaaa caaactccca 
     1321 aagctgaact ccaagccggg ccctgagggg ctccctggca tggcgctcgg ggcctctcgg 
     1381 ggagaagcct atgagccctt ggcgtggaac cctgaggagt ccactggggg gcctcattcg 
     1441 gccccaccga cagcacccag cccttttctg ttcagtgaga ccactgccat gaagtgcagg 
     1501 agccccggct gccccttcac actgaatgtg cagcacaacg gattttgtga acgttgccac 
     1561 aacgcccggc aacttcacgc cagccacgcc ccagaccaca caaggcactt ggatcccggg 
     1621 aagtgccaag cctgcctcca ggatgttacc aggacattta atgggatctg cagtacttgc 
     1681 ttcaaaagga ctacagcaga ggccgccgcc gccctcgcca ccgccctccc tcctgcctgt 
     1741 caccagcgtg ccaaggcaga tccctcgcgg ctcgtccgga gcccctcccc gcattcttgc 
     1801 cacagagctg gaaacgacgc ccctgctggc tgcctgtctc aagctgcacg gactcctggg 
     1861 gacaggacgg ggacgagcaa gtgcagaaaa gccggctgcg tgtattttgg gactccagaa 
     1921 aacaagggct tttgcacact gtgtttcatc gagtacagag aaaacaaaca ttttgctgct 
     1981 gcctcaggga aagtcagtcc cacagcgtcc aggttccaga acaccattcc gtgcctgggg 
     2041 agggaatgcg gcacccttgg aagcaccatg tttgaaggat actgccagaa gtgtttcatt 
     2101 gaagctcaga atcagagatt tcatgaggcc aaaaggacag aagagcaact gagatcgagc 
     2161 cagcgcagag atgtgcctcg aaccacacaa agcacctcaa ggcccaagtg cgcccgggcc 
     2221 tcctgcaaga acatcctggc ctgccgcagc gaggagctct gcatggagtg tcagcatccc 
     2281 aaccagagga tgggccctgg ggcccaccgg ggtgagcctg cccccgaaga cccccccaag 
     2341 cagcgttgcc gggcccccgc ctgtgatcat tttggcaatg ccaagtgcaa cggctactgc 
     2401 aacgaatgct ttcagttcaa gcagatgtat ggctaaccgg aaacaggtgg gtcacctcct 
     2461 gcaagaagtg gggcctcgag gtcgaggaat tcactcctca ggtgcaggct gcctatcaga 
     2521 aggtggtggc tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct 
     2581 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa 
     2641 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga 
     2701 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa 
     2761 catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata 
     2821 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga 
     2881 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac 
     2941 atgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc 
     3001 ttctcttatg gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg 
     3061 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 
     3121 aagtgtaaag cctgggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 
     3181 tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc 
     3241 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca 
     3301 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc 
     3361 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa 
     3421 gctaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 
     3481 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 
     3541 tcttatcatg tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 
     3601 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 
     3661 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcaggga 
     3721 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 
     3781 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 
     3841 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 
     3901 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 
     3961 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 
     4021 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 
     4081 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 
     4141 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 
     4201 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct 
     4261 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 
     4321 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 
     4381 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 
     4441 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 
     4501 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 
     4561 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 
     4621 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 
     4681 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 
     4741 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 
     4801 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 
     4861 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 
     4921 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 
     4981 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 
     5041 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 
     5101 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 
     5161 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 
     5221 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 
     5281 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 
     5341 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 
     5401 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 
     5461 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 
     5521 cacatttccc cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt 
     5581 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta 
     5641 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga 
     5701 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt 
     5761 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta 
     5821 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg 
     5881 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga 
     5941 gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt 
     6001 tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg gggccccccc 
     6061 caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggaaaggt gcggcggcag 
     6121 ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc 
     6181 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc 
     6241 cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac 
     6301 aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac 
     6361 ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt 
     6421 gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg 
     6481 gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc 
     6541 gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg 
     6601 aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg 
     6661 cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg 
     6721 cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt 
     6781 ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg 
     6841 aaggggcgcg gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat 
     6901 tgccttttat ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg 
     6961 gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc 
     7021 ggcgccggca ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc 
     7081 cttctccctc tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg 
     7141 ggcagggcgg ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat 
     7201 gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct 
     7261 catcattttg gcaaagaa 
//
