LOCUS       pRMCE\DV1               8893 bp    DNA     circular UNA 23-DEC-2019
DEFINITION  LMBP 8870.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 8870
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 23-DEC-2019
            NGS data implemented
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-13135037|
COMMENT     VNTDBDATE|-13135051|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pRMCE DV1|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        #1=(CRSite (CStringList) "PvuII" "cagctg" 4 0 4 1328 0 3692 0 5880 0 
COMMENT            8871 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 4 30 0 532 0 2996 0 
COMMENT            7500 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 4 3780 0 4085 0 5980 0 
COMMENT            7274 0 0 "")) 
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COMMENT        #23=(CFSignal (CObList) "SV40 polyA late" 43 0 1 5259 5355 0 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 
COMMENT                          1) 
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COMMENT                          "Created: 12/23/19 11:32" 1) 
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COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.genecorner@UGent.be</a>" 
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FEATURES             Location/Qualifiers
     misc_feature    35..277
                     /vntifkey="21"
                     /label=chicken\ß-globin\5'HS4\insulator\core\sequence
     misc_feature    278..520
                     /vntifkey="21"
                     /label=chicken\ß-globin\5'HS4\insulator\core\sequence
     misc_feature    1070..1117
                     /vntifkey="21"
                     /label=FRT\mutant
     CDS             complement(2323..3183)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     misc_feature    3801..3848
                     /vntifkey="21"
                     /label=FRT
     misc_feature    complement(5444..5477)
                     /vntifkey="21"
                     /label=loxP
     attenuator      complement(5533..5657)
                     /vntifkey="0"
                     /label=attR1
     attenuator      7113..7237
                     /vntifkey="0"
                     /label=attR2
     RBS             7281..7861
                     /vntifkey="32"
                     /label=5'\UTR\of\the\EMCV\polyprotein\gene\(IRES)
     CDS             7874..8593
                     /vntifkey="4"
                     /label=EGFP
     promoter        complement(1205..1288)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      complement(1380..1782)
                     /vntifkey="33"
                     /label=pMB1\ori
     misc_feature    complement(4031..4064)
                     /vntifkey="21"
                     /label=loxP
     promoter        539..1049
                     /vntifkey="29"
                     /label=mPGK1\promoter
     terminator      4751..4847
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(4751..4847)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     terminator      5005..5101
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      5259..5355
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(5005..5101)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     terminator      complement(5259..5355)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     promoter        5665..5717
                     /vntifkey="30"
                     /label=lacUV5\promoter
     CDS             5766..6425
                     /vntifkey="4"
                     /label=chloramphenicol\resistance
     CDS             6767..7072
                     /vntifkey="4"
                     /label=ccdB
     terminator      8624..8848
                     /vntifkey="43"
                     /label=BGH\polyA
     misc_feature    3865..3968
                     /vntifkey="21"
                     /label=SA
BASE COUNT     2181 a      2317 c      2214 g      2181 t 
ORIGIN
        1 cgcgccgggc cctcgcgact cgaggaataa attcgagctc acggggacag ccccccccca 
       61 aagcccccag ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg 
      121 ggctccgctc cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc 
      181 ccgggcacgg ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg 
      241 agcctgcaga cacctggggg atacggggaa aaagcttgag ctcacgggga cagccccccc 
      301 ccaaagcccc cagggatgta attacgtccc tcccccgcta gggggcagca gcgagccgcc 
      361 cggggctccg ctccggtccg gcgctccccc cgcatccccg agccggcagc gtgcggggac 
      421 agcccgggca cggggaaggt ggcacgggat cgctttcctc tgaacgcttc tcgctgctct 
      481 ttgagcctgc agacacctgg gggatacggg gaaaaagctt gtcgacgaat aaattcaatt 
      541 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc 
      601 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg 
      661 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct 
      721 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt 
      781 agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg 
      841 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg 
      901 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag 
      961 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc 
     1021 ctcttcctca tctccgggcc tttcgacctg caatcgccgc caccatggag aagttcctat 
     1081 tccgaagttc ctattcttca aaaggtatag gaacttccga tcgggatccc caatcctgca 
     1141 tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 
     1201 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 
     1261 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 
     1321 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 
     1381 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 
     1441 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 
     1501 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 
     1561 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 
     1621 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 
     1681 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 
     1741 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 
     1801 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 
     1861 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 
     1921 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 
     1981 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 
     2041 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 
     2101 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 
     2161 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 
     2221 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 
     2281 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 
     2341 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 
     2401 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 
     2461 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 
     2521 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 
     2581 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 
     2641 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 
     2701 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 
     2761 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 
     2821 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 
     2881 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 
     2941 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 
     3001 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 
     3061 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 
     3121 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 
     3181 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 
     3241 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 
     3301 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 
     3361 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca 
     3421 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 
     3481 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc 
     3541 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 
     3601 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg 
     3661 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 
     3721 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga 
     3781 attccacaaa agtgcgatcg gaagttccta ttccgaagtt cctattctct agaaagtata 
     3841 ggaacttctt aattaaggga tctgtagggc gcagtagtcc agggtttcct tgatgatgtc 
     3901 atacttatcc tgtccctttt ttttccacag ctcgcggttg aggacaaact cttcgcggtc 
     3961 tttccagtgg ggatcgacgg tatcgtagag tcgaggccgc tctagaacta gtggatccgg 
     4021 aacccttaat ataacttcgt ataatgtatg ctatacgaag ttattaggtc cctcgacctg 
     4081 caggaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta 
     4141 gcagccccgc tggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat 
     4201 ccaccggtag cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc 
     4261 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat 
     4321 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg 
     4381 ggtaggatca taatttaaac aagcaaaacc aaattaaggg ccagctcatt cctcccactc 
     4441 atgatctata gatctataga tctctcgtgg gatcattgtt tttctcttga ttcccacttt 
     4501 gtggttctaa gtactgtggt ttccaaatgt gtcagtttca tagcctgaag aacgagatca 
     4561 gcagcctctg ttccacatac acttcattct cagtattgtt ttgccaagtt ctaattccat 
     4621 cagaagcttg cagatctgcg actctagagg atctgcgact ctagaggatc ataatcagcc 
     4681 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc 
     4741 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt 
     4801 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 
     4861 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatctgc gactctagag 
     4921 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca 
     4981 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc 
     5041 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 
     5101 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat 
     5161 ctgcgactct agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta 
     5221 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt 
     5281 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 
     5341 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 
     5401 tatcatgtct ggatccccat caagctgatc cggaaccctt aatataactt cgtataatgt 
     5461 atgctatacg aagttattag gtccctcgac ctgcagccca agctagctta tcgataccgt 
     5521 cgacctcgaa tcacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa tgatataaat 
     5581 atcaatatat taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa 
     5641 catatccagt cactatggcg gccgcattag gcaccccagg ctttacactt tatgcttccg 
     5701 gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga gctaaggaag 
     5761 ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta 
     5821 aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc 
     5881 tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct 
     5941 ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag 
     6001 acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa 
     6061 ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca 
     6121 tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta 
     6181 ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa 
     6241 acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc 
     6301 aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct 
     6361 tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg 
     6421 cgtaaacgcg tggatccggc ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg 
     6481 atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca aaaagaggta 
     6541 tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt tgctcaaggc 
     6601 atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga agcccgtcgt 
     6661 ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt cgcccggttt 
     6721 attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc agtttaaggt 
     6781 ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat 
     6841 tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa 
     6901 agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac 
     6961 caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca 
     7021 ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg 
     7081 ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt tgtgttttac 
     7141 agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat 
     7201 tttacgtttc tcgttcagct ttcttgtaca aagtggtgat tcgaggtcga cggtatcgat 
     7261 aagcttgata tcgaattccg cccccccccc tctccctccc ccccccctaa cgttactggc 
     7321 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg 
     7381 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct 
     7441 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca 
     7501 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg 
     7561 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct 
     7621 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa 
     7681 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt 
     7741 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa 
     7801 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa 
     7861 tatggccaca accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct 
     7921 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg 
     7981 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt 
     8041 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc 
     8101 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga 
     8161 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga 
     8221 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa 
     8281 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga 
     8341 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag 
     8401 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct 
     8461 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg 
     8521 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga 
     8581 gctgtacaag taaagcggcc gcgagctcgc tgatcagcct cgactgtgcc ttctagttgc 
     8641 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc 
     8701 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct 
     8761 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg 
     8821 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg 
     8881 atcctctagt tgg 
//
