LOCUS       pCD-F-MALT1-S135        7878 bp    DNA     circular     18-OCT-2016
DEFINITION  LMBP 8941.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 8941
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)

COMMENT     VNTDATE|-11463246|
COMMENT     VNTNAME|pCD-F-MALT1-S135E|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTREPLTYPE|Phage
COMMENT     VNTREPLTYPE|Virus
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTEXTCHREPL|Animal/Other Eukaryotic
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCD-F-MALT1-S135E" 0 7878 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 0 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 7878 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #0=(CFSignal (CObList) "hCMV-IE promoter and enhancer" 29 0 0 235 818 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "209..863") 
COMMENT        #1=(CFSignal (CObList) "T7 promoter" 30 0 0 863 879 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "863..882") 
COMMENT        #2=(CFSignal (CObList) "SV40" 29 0 0 4222 4547 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "4222..4547") 
COMMENT        #3=(CFSignal (CObList) "Tn5 neomycin resistance" 4 0 0 4583 5377 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "4583..5377") 
COMMENT        #4=(CFSignal (CObList) "FLAG epitope" 22 0 0 923 946 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "920..946") 
COMMENT        #5=(CFSignal (CObList) "2506" 27 0 0 863 882 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "863..882") 
COMMENT        #6=(CFSignal (CObList) "BGH polyA" 43 0 0 3474 3698 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #7=(CFSignal (CObList) "5' UTR of lacZ, incl. RBS" 32 0 1 5726 5763 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #8=(CFSignal (CObList) "lac promoter" 30 0 1 5764 5847 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #9=(CFSignal (CObList) "pMB1 ori" 33 0 1 5939 6341 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #10=(CFSignal (CObList) "ampicillin resistance" 4 0 1 6882 7742 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #11=(CFSignal (CObList) "SV40 polyA early" 43 0 0 5551 5625 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #12=(CFSignal (CObList) "SV40 polyA late" 43 0 1 5551 5625 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #13=(CFSignal (CObList) "SV40 ori" 33 0 0 4416 4491 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #14=(CFSignal (CObList) "f1 ori" 33 0 0 3744 4172 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #15=(CFSignal (CObList) "S135E" 62 0 0 1373 1375 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #16=(CFSignal (CObList) "hMALT1 mutant" 4 0 0 971 3442 0 (CStringList) 
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COMMENT                          "DNA Plasmid Phage Virus 'pCD-F-MALT1-S135E'" 1) 
COMMENT                     #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 8941." 
COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: Peter Marynen" 
COMMENT                          1) 
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COMMENT                          "Created: 10/18/16 01:21" 1) 
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COMMENT                          "Last Modified: 10/18/16 01:21" 1) 
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COMMENT                          "length: 7878 bp" 1) 
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COMMENT                          "Replication Origin (3 total)") 
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FEATURES             Location/Qualifiers
     promoter        235..818
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     promoter        863..879
                     /vntifkey="30"
                     /label=T7\promoter
     promoter        4222..4547
                     /vntifkey="29"
                     /label=SV40
     CDS             4583..5377
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     misc_marker     923..946
                     /vntifkey="22"
                     /label=FLAG\epitope
     primer          863..882
                     /vntifkey="27"
                     /label=2506
     CDS             971..3442
                     /vntifkey="4"
                     /label=hMALT1\mutant
     terminator      3474..3698
                     /vntifkey="43"
                     /label=BGH\polyA
     RBS             complement(5726..5763)
                     /vntifkey="32"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
     promoter        complement(5764..5847)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      complement(5939..6341)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(6882..7742)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     terminator      5551..5625
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(5551..5625)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      4416..4491
                     /vntifkey="33"
                     /label=SV40\ori
     rep_origin      3744..4172
                     /vntifkey="33"
                     /label=f1\ori
     mutation        1373..1375
                     /vntifkey="62"
                     /label=S135E
                     /note="WT: TGA"
BASE COUNT     1970 a      1904 c      1968 g      2036 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 
      901 gtttaaactt aagcttacca tggattacaa ggacgatgac gataaggtac cgagctcgga 
      961 tcctgccacc atggcgctgt tgggggaccc gctacaggcc ctgccgccct cggccgcccc 
     1021 cacggggccg ctgctcgccc ctccggccgg cgcgaccctc aaccgcctgc gggagccgct 
     1081 gctgcggagg ctcagcgagc tcctggatca ggcgcccgag ggccggggct ggaggagact 
     1141 ggcggagctg gcggggagtc gcgggcgcct ccgcctcagt tgcctagacc tggagcagtg 
     1201 ttctcttaag gtactggagc ctgaaggaag ccccagcctg tgtctgctga agttaatggg 
     1261 tgaaaaaggt tgcacagtca cagaattgag tgatttcctg caggctatgg aacacactga 
     1321 agttcttcag cttctcagcc ccccaggaat aaagattact gtaaacccag aggaaaaggc 
     1381 agtcttggct ggacagtttg tgaaactgtg ttgccgggca actggacatc cttttgttca 
     1441 atatcagtgg ttcaaaatga ataaagagat tccaaatgga aatacatcag agcttatttt 
     1501 taatgcagtg catgtaaaag atgcaggctt ttatgtctgt cgagttaata acaatttcac 
     1561 ctttgaattc agccagtggt cacagctgga tgtttgcgac atcccagaga gcttccagag 
     1621 aagtgttgat ggcgtctctg aatccaagtt gcaaatctgt gttgaaccaa cttcccaaaa 
     1681 gctgatgcca ggcagcacat tggttttaca gtgtgttgct gttggaagcc ctattcctca 
     1741 ctaccagtgg ttcaaaaatg aattaccatt aacacatgag accaaaaagc tatacatggt 
     1801 gccttatgtg gatttggaac accaaggaac ctactggtgt catgtatata atgatcgaga 
     1861 cagtcaagat agcaagaagg tagaaatcat cataggaaga acagatgagg cagtggagtg 
     1921 cactgaagat gaattaaata atcttggtca tcctgataat aaagagcaaa caactgacca 
     1981 gcctttggcg aaggacaagg ttgccctttt gataggaaat atgaattacc gggagcaccc 
     2041 caagctcaaa gctcctttgg tggatgtgta cgaattgact aacttactga gacagctgga 
     2101 cttcaaagtg gtttcactgt tggatcttac tgaatatgag atgcgtaatg ctgtggatga 
     2161 gtttttactc cttttagaca agggagtata tgggttatta tattatgcag gacatggtta 
     2221 tgaaaatttt gggaacagct tcatggtccc cgttgatgct ccaaatccat ataggtctga 
     2281 aaattgtctg tgtgtacaaa atatactgaa attgatgcaa gaaaaagaaa ctggacttaa 
     2341 tgtgttctta ttggatatgt gtaggaaaag aaatgactac gatgatacca ttccaatctt 
     2401 ggatgcacta aaagtcaccg ccaatattgt gtttggatat gccacgtgtc aaggagctga 
     2461 agcttttgaa atccagcatt ctggattggc aaatggaatc tttatgaaat ttttaaaaga 
     2521 cagattatta gaagataaga aaatcactgt gttactggat gaagttgcag aagatatggg 
     2581 taagtgtcac cttaccaaag gcaaacaggc tctagagatt cgaagtagtt tatctgagaa 
     2641 gagagcactt actgatccaa tacagggaac agaatattct gctgaatctc ttgtgcggaa 
     2701 tctacagtgg gccaaggctc atgaacttcc agaaagtatg tgtcttaagt ttgactgtgg 
     2761 tgttcagatt caattaggat ttgcagctga gttttccaat gtcatgatca tctatacaag 
     2821 tatagtttac aaaccaccgg agataataat gtgtgatgcc tacgttactg attttccact 
     2881 tgatctagat attgatccaa aagatgcaaa taaaggcacg cctgaagaaa ctggcagcta 
     2941 cttggtatca aaggatcttc ccaagcattg cctctatacc agactcagtt cactgcaaaa 
     3001 attaaaggaa catctagtct tcacagtatg tttatcatat cagtactcag gattggaaga 
     3061 tactgtagag gacaagcagg aagtgaatgt tgggaaacct ctcattgcta aattagacat 
     3121 gcatcgaggt ttgggaagga agacttgctt tcaaacttgt cttatgtcta atggtcctta 
     3181 ccagagttct gcagccacct caggaggagc agggcattat cactcattgc aagacccatt 
     3241 ccatggtgtt taccattcac atcctggtaa tccaagtaat gttacaccag cagatagctg 
     3301 tcattgcagc cggactccag atgcatttat ttcaagtttc gctcaccatg cttcatgtca 
     3361 ttttagtaga agtaatgtgc cagtagagac aactgatgaa ataccattta gtttctctga 
     3421 caggctcaga atttctgaaa aagggcccgt ttaaacccgc tgatcagcct cgactgtgcc 
     3481 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg 
     3541 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag 
     3601 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga 
     3661 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag 
     3721 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 
     3781 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 
     3841 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 
     3901 catcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 
     3961 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 
     4021 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 
     4081 ctcggtctat tcttttgatt tataagggat tttggggatt tcggcctatt ggttaaaaaa 
     4141 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg 
     4201 tgtggaaagt ccccaggctc cccaggcagg cagaagtatg caaagcatgc atctcaatta 
     4261 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat 
     4321 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac 
     4381 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga 
     4441 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg 
     4501 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga 
     4561 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc 
     4621 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga 
     4681 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct 
     4741 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac 
     4801 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct 
     4861 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt 
     4921 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt 
     4981 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt 
     5041 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag 
     5101 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt 
     5161 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg 
     5221 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg 
     5281 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg 
     5341 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg 
     5401 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat 
     5461 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg 
     5521 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac 
     5581 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 
     5641 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc 
     5701 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 
     5761 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg 
     5821 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 
     5881 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 
     5941 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 
     6001 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 
     6061 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 
     6121 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 
     6181 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 
     6241 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 
     6301 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 
     6361 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 
     6421 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 
     6481 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 
     6541 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt 
     6601 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 
     6661 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 
     6721 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 
     6781 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 
     6841 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 
     6901 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 
     6961 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 
     7021 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 
     7081 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 
     7141 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 
     7201 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 
     7261 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 
     7321 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 
     7381 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 
     7441 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 
     7501 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 
     7561 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 
     7621 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 
     7681 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 
     7741 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 
     7801 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 
     7861 aaagtgccac ctgacgtc 
//
