LOCUS       pCAGGS-E-sciB             5860 bp    DNA     circular     17-OCT-2013
DEFINITION  LMBP 8982.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 8982
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 17-OCT-2013
            
COMMENT     VNTDATE|676116085|
COMMENT     VNTNAME|pCAGGS-E-sciB|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-E-sciB" 0 5860 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
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COMMENT       (CDocSetData 1 1 0 0 1 7 " Anneleen" 1 1 1 1 0 2 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "BglI" "EcoRI") (CStringList "atg" "gtg") 
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COMMENT        4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 3 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT            4239 0 4361 0 4432 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 3 4 0 659 0 1070 0 0 "")) 
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COMMENT        #2=(CFSignal (CObList) "intron AG promoter" 15 0 0 4885 5809 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "6303..7227") 
COMMENT        #3=(CFSignal (CObList) "ampicillin resistance" 4 0 1 3137 3997 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(4555..5415)") 
COMMENT        #4=(CFSignal (CObList) "pMB1 ori" 33 0 1 2195 2596 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(3613..4014)") 
COMMENT        #5=(CFSignal (CObList) "SV40 polyA late" 43 0 1 2006 2080 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(3424..3498)") 
COMMENT        #6=(CFSignal (CObList) "SV40 polyA early" 43 0 0 2006 2080 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "3424..3498") 
COMMENT        #7=(CFSignal (CObList) "SV40 ori" 33 0 0 1900 1975 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "3318..3393") 
COMMENT        #8=(CFSignal (CObList) "lac promoter" 30 0 1 1676 1758 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(3094..3176)") 
COMMENT        #9=(CFSignal (CObList) "3' UTR of rabbit \337-globin, incl. polyA" 43 0 
COMMENT            0 1150 1241 0 (CStringList) (CStringList) 1 1 1 1 "2568..2659") 
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COMMENT             (CStringList) 1 1 1 1 "16..54") 
COMMENT        #11=(CFSignal (CObList) "AG promoter" 29 0 0 4516 5857 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5934..7275") 
COMMENT        #12=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 4133 4510 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "5551..5928") 
COMMENT        #13=(CFSignal (CObList) "3' flanking region of rabbit \337-globin" 21 0 
COMMENT             0 1242 1598 0 (CStringList) (CStringList) 1 1 1 1 "2660..3016") 
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COMMENT                          "DNA Plasmid 'pCAGGS-E-sciB'" 1) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
COMMENT                          1) 
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COMMENT                          "Created: 10/17/13 10:01" 1) 
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COMMENT                          "length: 5860 bp" 1) 
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COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.lmbp@UGent.be</a>" 
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FEATURES             Location/Qualifiers
     intron          4885..5809
                     /vntifkey="15"
                     /label=intron\AG\promoter
     CDS             complement(3137..3997)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(2195..2596)
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      complement(2006..2080)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     terminator      2006..2080
                     /vntifkey="43"
                     /label=SV40\polyA\early
     rep_origin      1900..1975
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        complement(1676..1758)
                     /vntifkey="30"
                     /label=lac\promoter
     terminator      1150..1241
                     /vntifkey="43"
                     /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA
     misc_marker     16..54
                     /vntifkey="22"
                     /label=E-tag
     promoter        4516..5857
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     promoter        4133..4510
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
     misc_feature    1242..1598
                     /vntifkey="21"
                     /label=3'\flanking\region\of\rabbit\ß-globin
     CDS             64..1056
                     /vntifkey="4"
                     /label=SciB
BASE COUNT     1208 a      1595 c      1657 g      1400 t 
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 
       61 gctaccggcg ctctcaacca ggcgcagaaa accccctggc gttacggatt cctgaacctg 
      121 atgcggcgcg tggatgccca gctctgtgat actccggcag gcagtatctg gcagccacgg 
      181 atggaaaaat tccgtctggg acagacgccg acgatgacct ttgcgccacg ggaaatcgcc 
      241 caggtcagct ggcaggatgg acgcctgcac ctgagcctgt acagcctcgg actatggggg 
      301 ccaaacggcc cgttgccgct gcactatacc gagctggcac tgaaccgctc agagagtcgt 
      361 cacgatccga cgctggttca tttttccaat atttttcacc accgctggct gacgcagttc 
      421 tggcaggcat ggcgcagcgc ccagtccgct ggtggcgggc tggacaaacc ggaacacgac 
      481 cgttttgcgt tttatatcgc cagtctcagc gggcaggatc tacgggaaag cgccgaaagc 
      541 ccgctgtcag atcatgttcg cctggcagcc tccgcccatc tggtacgcga atcgcgcaat 
      601 cctgacgggc tggccgccac gctggcgcac tatttcagcg taccttttgc ggtgaaagaa 
      661 ttcgccctgc actggataac ggtggcgaac gatgaaatca cccgtctggg cacgcccgcg 
      721 cagtcttcgg tgctgggcaa cggggcgctt atcggccagg ccgtaccgga tatgcagtac 
      781 aaattccggc taattatcgg cccgctcacg ctggaggatt acctgcgttt tctgcccggc 
      841 ggcaacaatc tgcccgtgct gacagaactg gtgcgtacgt tcatcggttt tgaatattgc 
      901 tgggaaattg agctgcagtt gaagcctcat gccgcaccgc ctgcggtgat cggcggcgcg 
      961 cagcggcttg gctggacggc ctgggcaggt aaagccatgc atgaccgtcc ggtgaccggc 
     1021 atgatttttg aacctgaaca cggcttaacc cactgactcg aggtcgagga attcactcct 
     1081 caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa 
     1141 taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag 
     1201 catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt 
     1261 ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag 
     1321 tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg 
     1381 ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga 
     1441 aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta 
     1501 acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta 
     1561 ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca gcccaagctt 
     1621 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 
     1681 caacatacga gccggaagca taaagtgtaa agcctgggtg cctaatgagt gagctaactc 
     1741 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg 
     1801 atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc 
     1861 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 
     1921 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg 
     1981 gaggcctagg cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata 
     2041 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 
     2101 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc 
     2161 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 
     2221 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 
     2281 cggttatcca cagaatcagg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 
     2341 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 
     2401 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 
     2461 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 
     2521 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 
     2581 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 
     2641 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 
     2701 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 
     2761 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa 
     2821 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 
     2881 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 
     2941 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 
     3001 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 
     3061 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 
     3121 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 
     3181 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 
     3241 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 
     3301 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 
     3361 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 
     3421 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 
     3481 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 
     3541 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 
     3601 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 
     3661 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 
     3721 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 
     3781 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 
     3841 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 
     3901 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 
     3961 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 
     4021 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 
     4081 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggg tcgacattga 
     4141 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg 
     4201 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc 
     4261 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat 
     4321 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat 
     4381 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat 
     4441 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc 
     4501 gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc 
     4561 tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg 
     4621 gggggggggg gggggccccc cccaggcggg gcggggcggg gcgaggggcg gggcggggcg 
     4681 aggcggaaag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg 
     4741 gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgtt 
     4801 gcgcgctgcc ttccccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc 
     4861 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt 
     4921 aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag 
     4981 gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg 
     5041 tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc 
     5101 ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc 
     5161 ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg 
     5221 gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc 
     5281 cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg 
     5341 ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct 
     5401 cgggccgggg agggctcggg ggaaggggcg cggcggcccc cggagcgccg gcggctgtcg 
     5461 aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact 
     5521 tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca ccccctctag 
     5581 cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg 
     5641 tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc cgcgggggga 
     5701 cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg 
     5761 ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa 
     5821 cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa 
//

