LOCUS       pCAGGS-E-sciI           6373 bp    DNA     circular     19-NOV-2013
DEFINITION  LMBP 8987.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 8987
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 19-NOV-2013
            
COMMENT     VNTDATE|679060179|
COMMENT     VNTNAME|pCAGGS-E-sciI|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-E-sciI" 0 6373 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 7 " Anneleen" 1 1 1 1 0 2 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "AgeI" "ApaI" "AvaI" "BamHI" "BglI" "BglII" "BstXI" "DpnI" 
COMMENT         "DraI" "Eco47III" "EcoRI" "EcoRV" "HaeII" "HincII" "HindIII" "HpaI" 
COMMENT         "KpnI" "NarI" "NcoI" "NdeI" "NheI" "NotI" "NsiI" "PstI" "PvuI" "PvuII" 
COMMENT         "RsaI" "SacI" "SacII" "SalI" "ScaI" "SpeI" "SspI" "StuI" "XbaI" "XhoI" 
COMMENT         "XmaI" "XmnI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 2 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 6373 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "AgeI" "accggt" 2 0 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "ApaI" "gggccc" 6 0 2 5151 0 5510 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 6 1571 0 5530 0 5793 0 5913 
COMMENT            0 5929 0 6193 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 2313 0 2650 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 6 626 0 2452 0 3844 0 
COMMENT            4752 0 4874 0 4945 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "BglII" "agatct" 2 0 1 1663 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "BstXI" "ccannnnnntgg" 9 0 3 530 0 844 0 1642 0 
COMMENT            0 "") 
COMMENT        #7=(CRSite (CStringList) "DpnI" "gatc" 3 0 27 233 0 284 0 308 0 317 0 
COMMENT            480 0 594 0 1127 0 1200 0 1374 0 1519 0 1665 0 2110 0 2315 0 2652 0 
COMMENT            3399 0 3474 0 3485 0 3493 0 3571 0 3583 0 3688 0 4029 0 4047 0 4093 
COMMENT            0 4351 0 4368 0 4404 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "DraI" "tttaaa" 4 0 6 292 0 1312 0 1818 0 3590 
COMMENT            0 3609 0 4301 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "Eco47III" "agcgct" 4 0 4 268 0 1334 0 5441 0 
COMMENT            5603 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 4 0 1583 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "HaeII" "rgcgcy" 6 0 11 221 0 270 0 1336 0 
COMMENT             2710 0 3079 0 5443 0 5568 0 5605 0 5962 0 6077 0 6121 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "HincII" "gtyrac" 4 0 2 1366 0 4646 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 2129 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "HpaI" "gttaac" 4 0 0 0 "") 
COMMENT        #16=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 0 0 "") 
COMMENT        #17=(CRSite (CStringList) "NarI" "ggcgcc" 3 0 2 6074 0 6118 0 0 "") 
COMMENT        #18=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 3 12 0 2406 0 5022 0 0 "") 
COMMENT        #19=(CRSite (CStringList) "NdeI" "catatg" 3 0 4 1798 0 1858 0 1866 0 
COMMENT             4896 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "NheI" "gctagc" 2 0 1 764 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 57 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "NsiI" "atgcat" 6 0 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 2124 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 1 4094 0 0 "") 
COMMENT        #25=(CRSite (CStringList) "PvuII" "cagctg" 4 0 0 0 "") 
COMMENT        #26=(CRSite (CStringList) "RsaI" "gtac" 3 0 9 666 0 967 0 1244 0 4204 0 
COMMENT             4881 0 4906 0 4961 0 4994 0 5836 0 0 "") 
COMMENT        #27=(CRSite (CStringList) "SacI" "gagctc" 6 0 0 0 "") 
COMMENT        #28=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 3 195 0 5678 0 6207 0 0 
COMMENT             "") 
COMMENT        #29=(CRSite (CStringList) "SalI" "gtcgac" 2 0 2 1364 0 4644 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "ScaI" "agtact" 4 0 1 4204 0 0 "") 
COMMENT        #31=(CRSite (CStringList) "SpeI" "actagt" 2 0 1 4661 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "SspI" "aatatt" 4 0 1 4528 0 0 "") 
COMMENT        #33=(CRSite (CStringList) "StuI" "aggcct" 4 0 2 149 0 2498 0 0 "") 
COMMENT        #34=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 6276 0 0 "") 
COMMENT        #35=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 1571 0 0 "") 
COMMENT        #36=(CRSite (CStringList) "XmaI" "cccggg" 2 0 0 0 "") 
COMMENT        #37=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 3 1008 0 1478 0 4323 0 
COMMENT             0 "")) 
COMMENT       (CObList 
COMMENT        #38=(CFSignal (CObList) "intron AG promoter" 15 0 0 5398 6322 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "5402..6326") 
COMMENT        #39=(CFSignal (CObList) "ampicillin resistance" 4 0 1 3650 4510 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(3654..4514)") 
COMMENT        #40=(CFSignal (CObList) "pMB1 ori" 33 0 1 2708 3109 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2712..3113)") 
COMMENT        #41=(CFSignal (CObList) "SV40 polyA late" 43 0 1 2519 2593 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(2523..2597)") 
COMMENT        #42=(CFSignal (CObList) "SV40 polyA early" 43 0 0 2519 2593 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2523..2597") 
COMMENT        #43=(CFSignal (CObList) "SV40 ori" 33 0 0 2413 2488 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2417..2492") 
COMMENT        #44=(CFSignal (CObList) "lac promoter" 30 0 1 2189 2271 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2193..2275)") 
COMMENT        #45=(CFSignal (CObList) "3' UTR of rabbit \303\237-globin, incl. polyA" 
COMMENT             43 0 0 1663 1754 0 (CStringList) (CStringList) 1 1 1 1 "1667..1758") 
COMMENT        #46=(CFSignal (CObList) "E-tag" 22 0 0 16 54 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "16..54") 
COMMENT        #47=(CFSignal (CObList) "AG promoter" 29 0 0 5029 6370 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5033..6374") 
COMMENT        #48=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 4646 5023 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4650..5027") 
COMMENT        #49=(CFSignal (CObList) "3' flanking region of rabbit \303\237-globin" 
COMMENT             21 0 0 1755 2111 0 (CStringList) (CStringList) 1 1 1 1 "1759..2115") 
COMMENT        #50=(CFSignal (CObList) "Sci I" 4 0 0 64 1569 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "64..1573")) (CObList) (CObList) (CObList) 
COMMENT       (CObList) (CObList) (CObList) 
COMMENT       (CTextView 0 
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COMMENT                   5 "" 0 4) 
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COMMENT                          "DNA Plasmid 'pCAGGS-E-sciI'" 1) 
COMMENT                     #55=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 8987." 
COMMENT                          1) 
COMMENT                     #56=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #57=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 11/19/13 11:49" 1) 
COMMENT                     #58=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 11/19/13 11:52" 1) 
COMMENT                     #59=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6373 bp" 1) 
COMMENT                     #60=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #61=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #64=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 19-NOV-2013" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #65=(CFolderPar 
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COMMENT                          "BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #67=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.lmbp@UGent.be</a>" 
COMMENT                          1))) "Original Author") 
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COMMENT                   "Annotations") 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 64    End: 1569" 1) 
COMMENT                                   #74=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #75=(CLinePar 
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COMMENT                                        "   64..1573" 1))) "Sci I") 
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COMMENT                                        "Start: 3650  End: 4510 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #79=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(3654..4514)" 1))) 
COMMENT                                 "ampicillin resistance"))) "CDS (2 total)") 
COMMENT                     #80=(CFolderPar 
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COMMENT                           (CObjectList 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5398  End: 6322" 1) 
COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   5402..6326" 1))) 
COMMENT                                 "intron AG promoter"))) "Intron (1 total)") 
COMMENT                     #85=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #86=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1755 (3 #49# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1755  End: 2111" 1) 
COMMENT                                   #88=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #89=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1759..2115" 1))) 
COMMENT                                 "3' flanking region of rabbit \303\237-globin"))) 
COMMENT                          "Misc. Feature (1 total)") 
COMMENT                     #90=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 22 (7 22 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #91=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 16 (3 #46# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #92=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 16    End: 54  " 1) 
COMMENT                                   #93=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #94=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   16..54" 1))) "E-tag"))) 
COMMENT                          "Misc. Marker (1 total)") 
COMMENT                     #95=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #96=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4646 (3 #48# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #97=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4646  End: 5023" 1) 
COMMENT                                   #98=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #99=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4650..5027" 1))) "hCMV-IE enhancer") 
COMMENT                            #100=(CFolderPar 
COMMENT                                  (CGroupPar 
COMMENT                                   (CParagraph 5029 (3 #47# 0) 1 2 2 0 194) 
COMMENT                                   (CObjectList 
COMMENT                                    #101=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Start: 5029  End: 6370" 1) 
COMMENT                                    #102=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "chicken \303\237-actin/rabbit \303\237-globin hybrid promoter" 
COMMENT                                          1) 
COMMENT                                    #103=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #104=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   5033..6374" 1))) "AG promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT                                           "Start: 2189  End: 2271 (Complementary)" 
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COMMENT                                     #108=(CLinePar 
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COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #109=(CLinePar 
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COMMENT                                           "   complement(2193..2275)" 1))) 
COMMENT                                   "lac promoter"))) 
COMMENT                           "Promoter Prokaryotic (1 total)") 
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COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #114=(CLinePar 
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COMMENT                                           "   2417..2492" 1))) "SV40 ori") 
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COMMENT                                     #117=(CLinePar 
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COMMENT                                           "   complement(2712..3113)" 1))) 
COMMENT                                   "pMB1 ori"))) "Replication Origin (2 total)") 
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COMMENT                                           "   1667..1758" 1))) 
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COMMENT                                           "   complement(2523..2597)" 1))) 
COMMENT                                   "SV40 polyA late") 
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COMMENT                                     #131=(CLinePar 
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COMMENT                                           "   2523..2597" 1))) 
COMMENT                                   "SV40 polyA early"))) "Terminator (3 total)"))) 
COMMENT                   "Feature Map") 
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COMMENT                             (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "AgeI: 0 sites") 1 5 "ACCGGT" 
COMMENT                            "TGGCCA") 
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COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "EcoRV: 0 sites") 3 3 "GATATC" 
COMMENT                            "CTATAG") 
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COMMENT                            "CAATTG") 
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COMMENT                            (CFolderPar 
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COMMENT                              (CObjectList)) "KpnI: 0 sites") 5 1 "GGTACC" 
COMMENT                            "CCATGG") 
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COMMENT                            (CFolderPar 
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COMMENT                              (CObjectList)) "NsiI: 0 sites") 5 1 "ATGCAT" 
COMMENT                            "TACGTA") 
COMMENT                      #138=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "PvuII: 0 sites") 3 3 "CAGCTG" 
COMMENT                            "GTCGAC") 
COMMENT                      #139=(CRSFolderPar 
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COMMENT                            5 1 "GGGCCC" "CCCGGG") 
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COMMENT                            1 5 "GGATCC" "CCTAGG") 
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COMMENT                            1 5 "GAATTC" "CTTAAG") 
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COMMENT                             "HincII: 2 sites") 3 3 "GTYRAC" "CARYTG") 
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COMMENT                            1 5 "GTCGAC" "CAGCTG") 
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COMMENT                            1 5 "CCATGG" "GGTACC") 
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COMMENT                            4 2 "CCGCGG" "GGCGCC") 
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FEATURES             Location/Qualifiers
     intron          5398..6322
                     /vntifkey="15"
                     /label=intron\AG\promoter
     CDS             complement(3650..4510)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(2708..3109)
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      complement(2519..2593)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     terminator      2519..2593
                     /vntifkey="43"
                     /label=SV40\polyA\early
     rep_origin      2413..2488
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        complement(2189..2271)
                     /vntifkey="30"
                     /label=lac\promoter
     terminator      1663..1754
                     /vntifkey="43"
                     /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA
     misc_marker     16..54
                     /vntifkey="22"
                     /label=E-tag
     promoter        5029..6370
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     promoter        4646..5023
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
     misc_feature    1755..2111
                     /vntifkey="21"
                     /label=3'\flanking\region\of\rabbit\ß-globin
     CDS             64..1569
                     /vntifkey="4"
                     /label=Sci\I
BASE COUNT     1381 a      1718 c      1761 g      1513 t 
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 
       61 gctgcaaaca gtaatatgca ggcaaccgac gcggttgctc aggataccgc ctccgcatcc 
      121 ggtgaatttg atgcgttgct gaatcaggcc ttccgaccca agactaccca ggcggcaaaa 
      181 gccgtggaag ccgcggtgca gacgctggcg caacaggcgc tggcgaacac gatcaccgtc 
      241 agcgatgacg cctataaaag catcagcgct attattgcgc agatcgactt taaactgacc 
      301 gaacagatca aactgatcct gcaacatccc gactggcaga agctggaatc ctcgtggcgc 
      361 ggtatggagc atctggttta caacaccgag accgacgaaa agctgaaaat tcgcttcatg 
      421 aatctgtcaa aagatgaatt gcggcgcaac atgaagcgtt acaagggcat cgcctgggat 
      481 caaagcccga tgttcaagaa actgtatgaa gccgaatacg gccagttagg tggcgaacct 
      541 tatggctgta tcattgcgga ttactacttc gaccatacac cgcccgatgt ggatctgctt 
      601 ggctctatcg ccaaagtcgc cgcgtcggcc catgcgccgt ttattgccgg ggcttccccc 
      661 tcggtactgc aaatggactc ctggcaggaa ctggcgaatc cccgcgacct gaccaaaatc 
      721 gtcacccaga acctggaata tgcgccgtgg aactcgctgc gggctagcga agactcccgt 
      781 tatattggcc tgacgatgcc gcgttttctt gcccgcctgc cgtatggcgc aaaaaccaac 
      841 ccggtggacg agtttgattt tgaagaagat gcggatggtt ctgaccatac caaatacgtc 
      901 tggagcaacg cggcctacgc gatgggcgta aacatcaacc gttccttcaa acactacggc 
      961 tggtgtacgt tgattcgcgg tgtggaatca ggcggtgcgg tggaaaatct tccctgccat 
     1021 accttcccga ctgacgatgg cggcgtggac atgaaatgcc cgaccgaaat cgccatctct 
     1081 gaccgccgcg aggctgaact ggcgaaaaac ggttttatcc cgttgatcca ccgtaaaaac 
     1141 tcagactatg ccgcctttat cggcgcacag tcgctgcaaa aaccacagga atactacgat 
     1201 ccggacgcga cggccaacgc taacctgtct gcccgtctac cgtacctgtt cgcctgctcg 
     1261 cgcttcgctc acttcctcaa atgtatcgtc cgcgacaaaa tcggttcctt taaagagcgt 
     1321 gaggatatgc agcgctggct aaatgaatgg attatgaatt atgtcgacgc cgatccggtg 
     1381 aactcctcgc aagaaactaa agcccgtcgt ccgctggctg ccgctgaagt agtggtggaa 
     1441 gaggtcgaag gcaatccagg ttattacgac gcgaaattct tcctgcgtcc gcatttccag 
     1501 cttgaagggc tgacgggatc gctgcgcctg gtgacaaaac tgccgtcagt gaagcagggc 
     1561 aatgcctgac tcgaggtcga ggaattcact cctcaggtgc aggctgccta tcagaaggtg 
     1621 gtggctggtg tggccaatgc cctggctcac aaataccact gagatctttt tccctctgcc 
     1681 aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa 
     1741 tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat 
     1801 gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat 
     1861 gcccatatgc tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa 
     1921 cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt 
     1981 tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt 
     2041 ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc 
     2101 ttatggagat ccctcgacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc 
     2161 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 
     2221 taaagcctgg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc 
     2281 gctttccagt cgggaaacct gtcgtgccag cggatccgca tctcaattag tcagcaacca 
     2341 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc 
     2401 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg 
     2461 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctaa 
     2521 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 
     2581 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 
     2641 tcatgtctgg atccgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 
     2701 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 
     2761 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc agggataacg 
     2821 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 
     2881 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 
     2941 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 
     3001 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 
     3061 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 
     3121 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 
     3181 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 
     3241 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 
     3301 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 
     3361 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 
     3421 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 
     3481 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 
     3541 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 
     3601 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 
     3661 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 
     3721 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 
     3781 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 
     3841 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 
     3901 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 
     3961 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 
     4021 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 
     4081 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 
     4141 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 
     4201 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 
     4261 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 
     4321 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 
     4381 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 
     4441 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 
     4501 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 
     4561 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 
     4621 ttccccgaaa agtgccacct gggtcgacat tgattattga ctagttatta atagtaatca 
     4681 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta 
     4741 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat 
     4801 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg 
     4861 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac 
     4921 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt 
     4981 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc 
     5041 acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt 
     5101 attttttaat tattttgtgc agcgatgggg gcgggggggg ggggggggcc ccccccaggc 
     5161 ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga aaggtgcggc ggcagccaat 
     5221 cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat 
     5281 aaaaagcgaa gcgcgcggcg ggcgggagtc gttgcgcgct gccttccccc cgtgccccgc 
     5341 tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg 
     5401 agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt 
     5461 gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg 
     5521 gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc 
     5581 gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt 
     5641 gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg 
     5701 aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg 
     5761 gtcgggctgc aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg 
     5821 ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg 
     5881 caggtggggg tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggaaggg 
     5941 gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct 
     6001 tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct gtgcggagcc 
     6061 gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc 
     6121 cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct 
     6181 ccctctccag cctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag 
     6241 ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca 
     6301 tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca 
     6361 ttttggcaaa gaa 
//
