LOCUS       pCAGGS-E-sciO           6208 bp    DNA     circular      6-NOV-2013
DEFINITION  LMBP 8991.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 8991
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 6-NOV-2013
            
COMMENT     VNTDATE|677932557|
COMMENT     VNTNAME|pCAGGS-E-sciO|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-E-sciO" 0 6208 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 7 " Anneleen" 1 1 1 1 0 2 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "Eco47III" "NdeI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 3 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 6208 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "Eco47III" "agcgct" 4 0 3 222 0 5276 0 5438 0 0 
COMMENT            "") 
COMMENT        #1=(CRSite (CStringList) "NdeI" "catatg" 3 0 4 1633 0 1693 0 1701 0 4731 
COMMENT            0 0 "")) 
COMMENT       (CObList 
COMMENT        #2=(CFSignal (CObList) "intron AG promoter" 15 0 0 5233 6157 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "5237..6161") 
COMMENT        #3=(CFSignal (CObList) "ampicillin resistance" 4 0 1 3485 4345 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(3489..4349)") 
COMMENT        #4=(CFSignal (CObList) "pMB1 ori" 33 0 1 2543 2944 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2547..2948)") 
COMMENT        #5=(CFSignal (CObList) "SV40 polyA late" 43 0 1 2354 2428 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(2358..2432)") 
COMMENT        #6=(CFSignal (CObList) "SV40 polyA early" 43 0 0 2354 2428 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "2358..2432") 
COMMENT        #7=(CFSignal (CObList) "SV40 ori" 33 0 0 2248 2323 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "2252..2327") 
COMMENT        #8=(CFSignal (CObList) "lac promoter" 30 0 1 2024 2106 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2028..2110)") 
COMMENT        #9=(CFSignal (CObList) "3' UTR of rabbit \303\237-globin, incl. polyA" 
COMMENT            43 0 0 1498 1589 0 (CStringList) (CStringList) 1 1 1 1 "1502..1593") 
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COMMENT             (CStringList) 1 1 1 1 "16..54") 
COMMENT        #11=(CFSignal (CObList) "AG promoter" 29 0 0 4864 6205 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4868..6209") 
COMMENT        #12=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 4481 4858 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4485..4862") 
COMMENT        #13=(CFSignal (CObList) "3' flanking region of rabbit \303\237-globin" 
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COMMENT        #14=(CFSignal (CObList) "Sci O" 4 0 0 64 1404 0 (CStringList) 
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COMMENT                          "DNA Plasmid 'pCAGGS-E-sciO'" 1) 
COMMENT                     #19=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 8991." 
COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
COMMENT                          1) 
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COMMENT                          "Created: 11/06/13 10:35" 1) 
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COMMENT                          "Last Modified: 11/06/13 10:35" 1) 
COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6208 bp" 1) 
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COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.lmbp@UGent.be</a>" 
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FEATURES             Location/Qualifiers
     intron          5233..6157
                     /vntifkey="15"
                     /label=intron\AG\promoter
     CDS             complement(3485..4345)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(2543..2944)
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      complement(2354..2428)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     terminator      2354..2428
                     /vntifkey="43"
                     /label=SV40\polyA\early
     rep_origin      2248..2323
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        complement(2024..2106)
                     /vntifkey="30"
                     /label=lac\promoter
     terminator      1498..1589
                     /vntifkey="43"
                     /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA
     misc_marker     16..54
                     /vntifkey="22"
                     /label=E-tag
     promoter        4864..6205
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     promoter        4481..4858
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
     misc_feature    1590..1946
                     /vntifkey="21"
                     /label=3'\flanking\region\of\rabbit\ß-globin
     CDS             64..1404
                     /vntifkey="4"
                     /label=Sci\O
BASE COUNT     1301 a      1694 c      1743 g      1470 t 
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 
       61 gctagctgga atgaccgcgt agtctggagt gaaggacaat ttttactgcc gcagatgttt 
      121 cagcagcaag agcgttatct ggaacacgtc atgcattacc gcagcctgcc gctgaccccc 
      181 tttttctggg gattcagcca ctacaatatt gatggcgaag cgctgaacat cggtaaactg 
      241 atactgaaag aggcatcagg gatttttcct gacggcacgc cgtttaacgc accggaccac 
      301 accccgctgc cgccgccact gaccattctg ccggagcacc tgaaccagca gatttgtctg 
      361 gcggtaccgg tacgcgcgcc gaacagcgaa gaaaccacgt ttgacaataa cccggaatca 
      421 ttggcgcgtt tctcggtaca tgaacacgac atccgcgacg ccaactcgct gggacgtggc 
      481 gcgcagttat tacagctcag tcatctgcgc ctgcggctgc tgccggaaaa ggcggtgacg 
      541 ggcgcctgga ttggcctgcc gttgacccgc atcaccgggt tgaaccctga cgggcggata 
      601 gatatcgacc acgacctgat cccgcccatc attaattatc aggccagttc actgatgtgt 
      661 acctggctgt cgtggatcaa cgatctcatc cggatgcggg ccgattcgct ggcggaacgg 
      721 ctgaccggca gcgacaacca cggccatgaa gcagcggagg tctccgatta cctgctgctg 
      781 caaattctca atcgctttga gccgctgctg actcacctgg cgaaaacccc gctggccccg 
      841 gaggtgctgt accgctacct gtccgaactg gccggggaac tctccaccta tgtgcgtcca 
      901 caaacgcgac ggcccgctga atacaaagag tacaaacacc tgacgcccta tgccgggttg 
      961 aaatcgctgg ttgatgaggt gcagttcctg ctgaacgcgg tactgatccg gggcgcgcag 
     1021 cgcatcgagc tgaaagaggg gacttacggc atcctgaatg cggtggtggc cccttccgat 
     1081 cttgccgatt tcagcacgct ggtactggcg ataaaggctt caatgccgac cgatgtgcta 
     1141 ctgcaacatt ttgccgccca gaccaaaatc gggccatccg atcgcctgcc ggaactgatc 
     1201 cgctcgcatc tgccggggct ggctttgcag gttctgcctg taccaccgcg ccaaatcccg 
     1261 tttcaggccg gatacatcta ttacgacatc cgccgcgagg gagcattgtg ggaacacatt 
     1321 gcccgttacg gcgggatggc catgcatacc gccggggaat ttccggggct ggagacagaa 
     1381 ctgtggggag tgcgcgataa atgactcgag gtcgaggaat tcactcctca ggtgcaggct 
     1441 gcctatcaga aggtggtggc tggtgtggcc aatgccctgg ctcacaaata ccactgagat 
     1501 ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct 
     1561 ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca 
     1621 ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag 
     1681 agtttggcaa catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt 
     1741 catcagtata tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact 
     1801 tgaggttaga ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa 
     1861 ttttccttac atgttttact agccagattt ttcctcctct cctgactact cccagtcata 
     1921 gctgtccctc ttctcttatg gagatccctc gacctgcagc ccaagcttgg cgtaatcatg 
     1981 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 
     2041 cggaagcata aagtgtaaag cctgggtgcc taatgagtga gctaactcac attaattgcg 
     2101 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ccgcatctca 
     2161 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca 
     2221 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 
     2281 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct 
     2341 tttgcaaaaa gctaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 
     2401 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 
     2461 catcaatgta tcttatcatg tctggatccg ctgcattaat gaatcggcca acgcgcgggg 
     2521 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 
     2581 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 
     2641 gaatcaggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 
     2701 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 
     2761 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 
     2821 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 
     2881 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 
     2941 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 
     3001 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 
     3061 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 
     3121 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta 
     3181 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 
     3241 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 
     3301 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 
     3361 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 
     3421 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 
     3481 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 
     3541 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 
     3601 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 
     3661 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 
     3721 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 
     3781 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 
     3841 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 
     3901 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 
     3961 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 
     4021 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 
     4081 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 
     4141 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 
     4201 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 
     4261 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 
     4321 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 
     4381 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 
     4441 gggttccgcg cacatttccc cgaaaagtgc cacctgggtc gacattgatt attgactagt 
     4501 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt 
     4561 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg 
     4621 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg 
     4681 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt 
     4741 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg 
     4801 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg 
     4861 gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca 
     4921 attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg 
     4981 gggccccccc caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggaaaggt 
     5041 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg 
     5101 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgttgc gcgctgcctt 
     5161 ccccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg 
     5221 ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg 
     5281 gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag 
     5341 ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc 
     5401 gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt 
     5461 tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg 
     5521 gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg 
     5581 ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag 
     5641 cacggcccgg cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg 
     5701 ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag 
     5761 ggctcggggg aaggggcgcg gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag 
     5821 ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc ctttgtccca 
     5881 aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc 
     5941 gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg 
     6001 ccgccgtccc cttctccctc tccagcctcg gggctgtccg cggggggacg gctgccttcg 
     6061 ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct ctagagcctc 
     6121 tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat 
     6181 tgtgctgtct catcattttg gcaaagaa 
//
