LOCUS       pdcDNA-R4R3-dhCMV        6125 bp    DNA     circular      4-FEB-2014
DEFINITION  LMBP 8998.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 8998
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 4-FEB-2014
COMMENT     VNTDATE|688403198|
COMMENT     VNTNAME|pdcDNA-R4R3-dhCMV|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pdcDNA-R4R3-dhCMV" 0 6125 
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COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
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COMMENT       (CMolPar 0 0 1 2388 2391 1 6125 0 0 0 0 0 0 0 0) (CStringList) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 1272 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "HaeII" "rgcgcy" 6 0 5 2141 0 2149 0 2963 0 
COMMENT            4189 0 4559 0 0 "")) 
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COMMENT        #3=(CFSignal (CObList) "Tn5 neomycin resistance" 4 0 0 2831 3625 0 
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COMMENT        #4=(CFSignal (CObList) "ampicillin resistance" 4 0 1 5130 5990 0 
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COMMENT        #5=(CFSignal (CObList) "SP6 promoter" 30 0 1 1753 1769 0 (CStringList) 
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COMMENT        #6=(CFSignal (CObList) "BGH polyA" 43 0 0 1795 2019 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1795..2019") 
COMMENT        #7=(CFSignal (CObList) "f1 ori" 33 0 0 2065 2493 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "2065..2493") 
COMMENT        #8=(CFSignal (CObList) "SV40 early promoter" 29 0 0 2498 2707 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "2498..2707") 
COMMENT        #9=(CFSignal (CObList) "SV40 polyA early" 43 0 0 3799 3873 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "3799..3873") 
COMMENT        #10=(CFSignal (CObList) "SV40 polyA late" 43 0 1 3799 3873 0 
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COMMENT        #11=(CFSignal (CObList) "pMB1 ori" 33 0 1 4187 4589 0 (CStringList) 
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COMMENT        #12=(CFSignal (CObList) "lac promoter" 30 0 1 4012 4095 0 (CStringList) 
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COMMENT        #13=(CFSignal (CObList) "attR4" 0 0 1 18 142 0 (CStringList) 
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COMMENT        #16=(CFSignal (CObList) "chloramphenicol resistance" 4 0 1 830 1489 0 
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COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
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COMMENT                          "Created: 01/31/14 03:06" 1) 
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FEATURES             Location/Qualifiers
     rep_origin      2664..2739
                     /vntifkey="33"
                     /label=SV40\ori
     CDS             2831..3625
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
                     /note="Tn5 antibiotic resistance gene; coding sequence"
     CDS             complement(5130..5990)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        complement(1753..1769)
                     /vntifkey="30"
                     /label=SP6\promoter
     terminator      1795..2019
                     /vntifkey="43"
                     /label=BGH\polyA
     rep_origin      2065..2493
                     /vntifkey="33"
                     /label=f1\ori
     promoter        2498..2707
                     /vntifkey="29"
                     /label=SV40\early\promoter
     terminator      3799..3873
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(3799..3873)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(4187..4589)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        complement(4012..4095)
                     /vntifkey="30"
                     /label=lac\promoter
     attenuator      complement(18..142)
                     /vntifkey="0"
                     /label=attR4
     attenuator      1598..1722
                     /vntifkey="0"
                     /label=attR3
     CDS             complement(183..488)
                     /vntifkey="4"
                     /label=ccdB
     CDS             complement(830..1489)
                     /vntifkey="4"
                     /label=chloramphenicol\resistance
     promoter        complement(1538..1590)
                     /vntifkey="30"
                     /label=lacUV5\promoter
BASE COUNT     1474 a      1557 c      1489 g      1605 t 
ORIGIN
        1 gacggatcgg gagatcttca actttgtata gaaaagttga acgagaaacg taaaatgata 
       61 taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa 
      121 cacaacatat ccagtcacta tggtcgacct gcagactggc tgtgtataag ggagcctgac 
      181 atttatattc cccagaacat caggttaatg gcgtttttga tgtcattttc gcggtggctg 
      241 agatcagcca cttcttcccc gataacggag accggcacac tggccatatc ggtggtcatc 
      301 atgcgccagc tttcatcccc gatatgcacc accgggtaaa gttcacgggg gactttatct 
      361 gacagcagac gtgcactggc cagggggatc accatccgtc gcccgggcgt gtcaataata 
      421 tcactctgta catccacaaa cagacgataa cggctctctc ttttataggt gtaaacctta 
      481 aactgcattt caccagcccc ggttctcgtc ggcaaaagag ccgttcattt caataaaccg 
      541 ggcgacctca gccatccctt cttgattttc cgctttccag cgttcggcac gcagacgacg 
      601 ggcttcattc tgcatggttg tgcttaccga accggagata ctgacatcat atatgccttg 
      661 agcaactgat agctgtcgct gtcaactgtc actgtaatac gctgcttcat agcatacctc 
      721 tttttgacat acttcgggta tacatatcag tatatattct tataccgcaa aaatcagcgc 
      781 gcaaatacgc atactgttat ctggctttta gtaagccgga tcctctagat tacgccccgc 
      841 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat 
      901 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat 
      961 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat 
     1021 caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc 
     1081 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta 
     1141 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct 
     1201 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca 
     1261 ttgccatacg gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg 
     1321 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa 
     1381 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat 
     1441 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct 
     1501 tagctcctga aaatctcgac ggatcctaac tcaaaatcca cacattatac gagccggaag 
     1561 cataaagtgt aaagcctggg gtgcctaatg cggccgccat agtgactgga tatgttgtgt 
     1621 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat 
     1681 atcattttac gtttctcgtt caactttatt atacatagtt gagctcgagc atgcatctag 
     1741 agggccctat tctatagtgt cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc 
     1801 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag 
     1861 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta 
     1921 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag 
     1981 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca 
     2041 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg 
     2101 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg 
     2161 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg 
     2221 gcatcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt 
     2281 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt 
     2341 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta 
     2401 tctcggtcta ttcttttgat ttataaggga ttttggggat ttcggcctat tggttaaaaa 
     2461 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg 
     2521 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 
     2581 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc 
     2641 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 
     2701 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg 
     2761 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg 
     2821 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga 
     2881 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt 
     2941 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct 
     3001 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg 
     3061 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt 
     3121 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc 
     3181 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc 
     3241 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga 
     3301 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg 
     3361 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat 
     3421 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg 
     3481 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc 
     3541 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta 
     3601 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg 
     3661 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc 
     3721 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg 
     3781 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat 
     3841 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 
     3901 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg 
     3961 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac 
     4021 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca 
     4081 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 
     4141 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc 
     4201 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 
     4261 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 
     4321 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 
     4381 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 
     4441 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 
     4501 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 
     4561 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 
     4621 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 
     4681 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 
     4741 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 
     4801 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 
     4861 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 
     4921 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 
     4981 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 
     5041 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 
     5101 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 
     5161 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 
     5221 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 
     5281 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 
     5341 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 
     5401 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 
     5461 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 
     5521 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 
     5581 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 
     5641 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 
     5701 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 
     5761 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 
     5821 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 
     5881 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 
     5941 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 
     6001 tttcaatatt attgagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 
     6061 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 
     6121 acgtc 
//
