LOCUS       pDONR-C-attP2rP3        6244 bp    DNA     circular     28-FEB-2014
DEFINITION  LMBP 9010.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 9010
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 28-FEB-2014
COMMENT     VNTDATE|690916560|
COMMENT     VNTNAME|pDONR-C-attP2rP3|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pDONR-C-attP2rP3" 0 6244 
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COMMENT         "DraI" "Eco47III" "EcoRI" "EcoRV" "HaeII" "HincII" "HindIII" "HpaI" 
COMMENT         "KpnI" "NarI" "NcoI" "NdeI" "NheI" "NotI" "NsiI" "PstI" "PvuI" "PvuII" 
COMMENT         "RsaI" "SacI" "SacII" "SalI" "ScaI" "SpeI" "SspI" "StuI" "XbaI" "XhoI" 
COMMENT         "XmaI" "XmnI") (CStringList "atg" "gtg") 
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COMMENT            (CStringList) 1 1 1 1 "4759..5161") 
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COMMENT        #2=(CFSignal (CObList) "attP2R" 0 0 1 2234 2465 0 (CStringList) 
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COMMENT        #3=(CFSignal (CObList) "ccdB" 4 0 1 667 972 0 (CStringList) 
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COMMENT        #5=(CFSignal (CObList) "streptomycin/spectinomycin resistance (aadA)" 4 
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COMMENT                         "Created: 02/28/14 05:16" 1) 
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COMMENT                         "Last Modified: 02/28/14 05:16" 1) 
COMMENT                    #17=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "length: 6244 bp" 1) 
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COMMENT                                   "chloramphenicol resistance" "@N" 1 20.4164 
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FEATURES             Location/Qualifiers
     rep_origin      4759..5161
                     /vntifkey="33"
                     /label=pMB1\ori
     attenuator      complement(79..310)
                     /vntifkey="0"
                     /label=attP3
     attenuator      complement(2234..2465)
                     /vntifkey="0"
                     /label=attP2R
     CDS             complement(667..972)
                     /vntifkey="4"
                     /label=ccdB
     CDS             complement(1314..1973)
                     /vntifkey="4"
                     /label=chloramphenicol\resistance
     CDS             3135..4145
                     /vntifkey="4"
                     /label=streptomycin/spectinomycin\resistance\(aadA)
     promoter        23..39
                     /vntifkey="30"
                     /label=T7\promoter
BASE COUNT     1588 a      1555 c      1502 g      1599 t 
ORIGIN
        1 tcaggaaaca gctatgacca tgtaatacga ctcactatag gggatatcgc ggccgcgtta 
       61 acgctaccat ggagctccaa ataatgattt tattttgact gatagtgacc tgttcgttgc 
      121 aacaaattga taagcaatgc ttttttataa tgccaacttt gtataataaa gttgaacgag 
      181 aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta 
      241 cataatactg taaaacacaa catatgcagt cactatgaat caactactta gatggtatta 
      301 gtgacctgta gaattcgagc tcgaccgaca gccttccaaa tgttcttctc aaacggaatc 
      361 gtcgtatcca gcctactcgc tattgtcctc aatgccgtat taaatcataa aaagaaataa 
      421 gaaaaagagg tgcgagcctc ttttttgtgt gacaaaataa aaacatctac ctattcatat 
      481 acgctagtgt catagtcctg aaaatcatct gcatcaagaa caatttcaca actcttatac 
      541 ttttctctta caagtcgttc ggcttcatct ggattttcag cctctatact tactaaacgt 
      601 gataaagttt ctgtaatttc tactgtatcg acctgcagac tggctgtgta taagggagcc 
      661 tgacatttat attccccaga acatcaggtt aatggcgttt ttgatgtcat tttcgcggtg 
      721 gctgagatca gccacttctt ccccgataac ggagaccggc acactggcca tatcggtggt 
      781 catcatgcgc cagctttcat ccccgatatg caccaccggg taaagttcac gggagacttt 
      841 atctgacagc agacgtgcac tggccagggg gatcaccatc cgtcgcccgg gcgtgtcaat 
      901 aatatcactc tgtacatcca caaacagacg ataacggctc tctcttttat aggtgtaaac 
      961 cttaaactgc atttcaccag tccctgttct cgtcagcaaa agagccgttc atttcaataa 
     1021 accgggcgac ctcagccatc ccttcctgat tttccgcttt ccagcgttcg gcacgcagac 
     1081 gacgggcttc attctgcatg gttgtgctta ccagaccgga gatattgaca tcatatatgc 
     1141 cttgagcaac tgatagctgt cgctgtcaac tgtcactgta atacgctgct tcatagcaca 
     1201 cctctttttg acatacttcg ggtatacata tcagtatata ttcttatacc gcaaaaatca 
     1261 gcgcgcaaat acgcatactg ttatctggct tttagtaagc cggatccacg cgattacgcc 
     1321 ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag 
     1381 ccatcacaga cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc 
     1441 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt 
     1501 aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata 
     1561 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg 
     1621 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt 
     1681 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct 
     1741 ttcattgcca tacggaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag 
     1801 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc 
     1861 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta 
     1921 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct 
     1981 tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca 
     2041 ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt ctcattttcg ccaaaagttg 
     2101 gcccagggct tcccggtatc aacagggaca ccaggattta tttattctgc gaagtgatct 
     2161 tccgtcacag gtatttattc ggcgcaaagt gcgtcgggtg atgctgccaa cttagtcgac 
     2221 gctctagagc tccaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa 
     2281 attgataagc aatgcttttt tataatgcca actttgtaca agaaagttga acgagaaacg 
     2341 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa 
     2401 tactgtaaaa cacaacatat gcagtcacta tgaatcaact acttagatgg tattagtgac 
     2461 ctgtagaatt cgagctctag agctgcaggg cccgagctta agactggccg tcgttttacg 
     2521 gtaccgcgat cgctcgcgac ctgcaggcat aaagccgtca gtgtccgcat aaagaaccac 
     2581 ccataatacc cataatagct gtttgccatc gctaccttag gaccgttata gttaattacc 
     2641 ctgttatccc tattaattaa gagctcgcta ccttaagaga ggatatcggc gcgcctctag 
     2701 aatttaaatg gatcctacgt actcgaggaa ttcaattcgg cgttaattca gtacattaaa 
     2761 aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc acaatatatc 
     2821 ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc accactcgat 
     2881 acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag gcggcagact 
     2941 ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg tttgaaacac 
     3001 ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg 
     3061 atcaattcgg gcacgaaccc agtggacata agcctcgttc ggttcgtaag ctgtaatgca 
     3121 agtagcgtaa ctgccgtcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa 
     3181 cggcgcagtg gcggttttca tggcttcttg ttatgacatg tttttttggg gtacagtcta 
     3241 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga 
     3301 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatggggg 
     3361 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc 
     3421 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga 
     3481 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc 
     3541 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc 
     3601 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag 
     3661 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg 
     3721 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg 
     3781 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat 
     3841 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg 
     3901 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg 
     3961 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg 
     4021 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg 
     4081 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca 
     4141 aataatgtct agctagaaat tcgttcaagc cgacgccgct tcgccggcgt taactcaagc 
     4201 gattagatgc actaagcaca taattgctca cagccaaact atcaggtcaa gtctgctttt 
     4261 attattttta agcgtgcata ataagcccta cacaaattgg gagatatatc atgcatgacc 
     4321 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 
     4381 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 
     4441 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 
     4501 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc 
     4561 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 
     4621 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 
     4681 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 
     4741 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 
     4801 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 
     4861 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 
     4921 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 
     4981 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 
     5041 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat 
     5101 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag 
     5161 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc 
     5221 actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc 
     5281 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac 
     5341 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca 
     5401 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc ttgatgtggg 
     5461 cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt gcctggccgt 
     5521 aggccagcca tttttgagcg gccagcggcc gcgataggcc gacgcgaagc ggcggggcgt 
     5581 agggagcgca gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt gcgctggcca 
     5641 gacagttatg cacaggccag gcgggtttta agagttttaa taagttttaa agagttttag 
     5701 gcggaaaaat cgcctttttt ctcttttata tcagtcactt acatgtgtga ccggttccca 
     5761 atgtacggct ttgggttccc aatgtacggg ttccggttcc caatgtacgg ctttgggttc 
     5821 ccaatgtacg tgctatccac aggaaagaga ccttttcgac ctttttcccc tgctagggca 
     5881 atttgcccta gcatctgctc cgtacattag gaaccggcgg atgcttcgcc ctcgatcagg 
     5941 ttgcggtagc gcatgactag gatcgggcca gcctgccccg cctcctcctt caaatcgtac 
     6001 tccggcaggt catttgaccc gatcagcttg cgcacggtga aacagaactt cttgaactct 
     6061 ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc atctggcttc tgccttgcct 
     6121 gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat caaaaagtaa 
     6181 tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg gtacatccaa 
     6241 tcag 
//
