LOCUS       pACM44        5147 bp    DNA     circular     12-MAR-2014
DEFINITION  LMBP 9067.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|692185896|
COMMENT     VNTNAME|pACM44|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES             Location/Qualifiers
     repeat_region   34..56
                     /vntifkey="34"
                     /label=IRR
                     /note="Inverted Repeat-Right of IS1"
     CDS             complement(996..1469)
                     /vntifkey="4"
                     /label=dfrA1
                     /note="dihydrofolate reductase for trimethoprim resistance"
     CDS             complement(112..903)
                     /vntifkey="4"
                     /label=aadA1
                     /note="3''-O-adenyltransferase for Streptomycin/Spectinomycin resistance. Copy 2"
     rep_origin      2928..2928
                     /vntifkey="33"
                     /label=ORI
                     /note=" RNaseH cleavage point "
     promoter        complement(2568..2568)
                     /vntifkey="30"
                     /label=P(LAC)
                     /note=" lac promoter "
     CDS             complement(3690..4547)
                     /vntifkey="4"
                     /label=AP(R)
                     /note=" bla gene- Ap(r) determinant "
     promoter        complement(4582..4582)
                     /vntifkey="30"
                     /label=P(BLA)
                     /note=" bla gene promoter "
     CDS             1626..2475
                     /vntifkey="4"
                     /label=intI1-partial
                     /note="integrase gene intI1 truncated by cloning at NruI site"
BASE COUNT     1214 a      1340 c      1321 g      1272 t 
ORIGIN
        1 aagtcatcgg gcattatctg aacataaaac actatcaata agttggagtc attacccgct 
       61 tgagttaagc cgcgccgcga agcggcgtcg gcttgaacga attgttagac attatttgcc 
      121 gactaccttg gtgatctcgc ctttcacgta gtgaacaaat tcttccaact gatctgcgcg 
      181 cgaggccaag cgatcttctt cttgtccaag ataagcctgt ctagcttcaa gtatgacggg 
      241 ctgatactgg gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat 
      301 tctgccggtt actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc 
      361 gccagcccag tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag 
      421 atcctgttca ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct 
      481 atgttctctt gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa 
      541 gatacctgca agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg 
      601 ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg acttctacag cgcggagaat 
      661 ctcgctctct ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt 
      721 tgtttcatca agccttacgg tcaccgtaac cagcaaatca atatcactgt gtggcttcag 
      781 gccgccatcc actgcggagc cgtacaaatg tacggccagc aacgtcggtt cgagatggcg 
      841 ctcgatgacg ccaactacct ctgatagttg agtcgatact tcggcgatca ccgcttccct 
      901 catgatgttt aacgcctggc acagcggatc gcaaacctgg cgcggctttt ggtacaaaag 
      961 gcgtgacagg tttgcgaatc cgttgctgcc acttgttaac ccttttgcca gatttggtaa 
     1021 ctataattta tgttagaggc gaagtcttgg gtaaaaactg gcctaaaatt gctggggatt 
     1081 tcaggaaagt aaacatcacc ttccggctcg atgtctattg tagatatatg tagtgtatct 
     1141 acttgatcga tcaggctttt gtatatctcc ccaccacctg aaacaatgac atgatccgtt 
     1201 attttcttta ggttggttaa agcatcttta attgatggaa agatcactac gttctcattg 
     1261 tcagatgtaa aacttgaacg tgttacgacc gcatactttc ggttgggtaa tgctcccatt 
     1321 gattcaaaag tcttgcgtcc aaccaacagc cattggttat aggtaatagc tttaaacagg 
     1381 agctgttcac ctttggcact ccatggaata tcagggccat tcccgataac tccattcttc 
     1441 gatatagcta ccattagtga tagtttcaca attcttcctc agaggttaac tttgttttag 
     1501 ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg 
     1561 cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa cagtcataac 
     1621 aagccatgaa aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc 
     1681 agttgcgtga gcgcatacgc tacttgcatt acagcttacg aaccgaacag gcttatgtcc 
     1741 actgggttcg tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc ttgggcagca 
     1801 gcgaagtcga ggcatttctg tcctggctgg cgaacgagcg caaggtttcg gtctccacgc 
     1861 atcgtcaggc attggcggcc ttgctgttct tctacggcaa ggtgctgtgc acggatctgc 
     1921 cctggcttca ggagatcgga agacctcggc cgtcgcggcg cttgccggtg gtgctgaccc 
     1981 cggatgaagt ggttcgcatc ctcggttttc tggaaggcga gcatcgtttg ttcgcccagc 
     2041 ttctgtatgg aacgggcatg cggatcagtg agggtttgca actgcgggtc aaggatctgg 
     2101 atttcgatca cggcacgatc atcgtgcggg agggcaaggg ctccaaggat cgggccttga 
     2161 tgttacccga gagcttggca cccagcctgc gcgagcagct gtcgcgtgca cgggcatggt 
     2221 ggctgaagga ccaggccgag ggccgcagcg gcgttgcgct tcccgacgcc cttgagcgga 
     2281 agtatccgcg cgccgggcat tcctggccgt ggttctgggt ttttgcgcag cacacgcatt 
     2341 cgaccgatcc acggagcggt gtcgtgcgtc gccatcacat gtatgaccag acctttcagc 
     2401 gcgccttcaa acgtgccgta gaacaagcag gcatcacgaa gcccgccaca ccgcacaccc 
     2461 tccgccactc gttcggggga tcctctagag tcgacctgca ggcatgcaag cttggcgtaa 
     2521 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata 
     2581 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta 
     2641 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa 
     2701 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg 
     2761 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 
     2821 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 
     2881 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 
     2941 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 
     3001 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 
     3061 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 
     3121 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 
     3181 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 
     3241 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 
     3301 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 
     3361 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 
     3421 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 
     3481 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 
     3541 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 
     3601 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 
     3661 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 
     3721 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 
     3781 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 
     3841 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 
     3901 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 
     3961 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 
     4021 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 
     4081 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 
     4141 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 
     4201 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 
     4261 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 
     4321 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 
     4381 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 
     4441 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 
     4501 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 
     4561 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 
     4621 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 
     4681 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc 
     4741 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag 
     4801 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca 
     4861 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg 
     4921 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc 
     4981 aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct 
     5041 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg 
     5101 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaatt 
//
