LOCUS       pENTR223-ORF      3912 bp    DNA     circular UNA 22-OCT-2021
DEFINITION  LMBP 9210.
COMMENT     Accession number: LMBP 9210
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 22-OCT-2021
FEATURES             Location/Qualifiers
     rep_origin      join(3632..3912,1..122)
                     /label=pMB1\ori
     terminator      complement(268..295)
                     /label=T2\terminator
     terminator      427..470
                     /label=T1\terminator
     misc_recomb     570..667
                     /label=attL1
     CDS             668..1791
                     /label=hORF
     misc_recomb     complement(1792..1889)
                     /label=attL2
     promoter        complement(1910..1926)
                     /label=T7\promoter
     CDS             2159..3169
                     /label=spectinomycin\resistance\(aadA)
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
      601 CTGTTCGTTG CAACAAATTG ATAAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
      661 AGTTGGCATG NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
      721 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
      781 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
      841 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
      901 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
      961 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
     1021 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
     1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
     1141 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
     1201 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
     1261 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
     1321 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
     1381 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
     1441 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
     1501 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
     1561 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
     1621 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
     1681 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
     1741 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNT RCCCAACTTT
     1801 CTTGTACAAA GTTGGCATTA TAAGAAAGCA TTGCTTATCA ATTTGTTGCA ACGAACAGGT
     1861 CACTATCAGT CAAAATAAAA TCATTATTTG CCATCCAGCT GATATCCCCT ATAGTGAGTC
     1921 GTATTACATG GTCATAGCTG TTTCCTGGCA GCTCTGGCCC GTGTCTCAAA ATCTCTGATG
     1981 TTACATTGCA CAAGATAAAA ATATATCATC ATGCCTCCTC TAGACCAGCC AGGACAGAAA
     2041 TGCCTCGACT TCGCTGCTGC CCAAGGTTGC CGGGTGACGC ACACCGTGGA AACGGATGAA
     2101 GGCACGAACC CAGTGGACAT AAGCCTGTTC GGTTCGTAAG CTGTAATGCA AGTAGCGTAT
     2161 GCGCTCACGC AACTGGTCCA GAACCTTGAC CGAACGCAGC GGTGGTAACG GCGCAGTGGC
     2221 GGTTTTCATG GCTTGTTATG ACTGTTTTTT TGGGGTACAG TCTATGCCTC GGGCATCCAA
     2281 GCAGCAAGCG CGTTACGCCG TGGGTCGATG TTTGATGTTA TGGAGCAGCA ACGATGTTAC
     2341 GCAGCAGGGC AGTCGCCCTA AAACAAAGTT AAACATCATG AGGGAAGCGG TGATCGCCGA
     2401 AGTATCGACT CAACTATCAG AGGTAGTTGG CGTCATCGAG CGCCATCTCG AACCGACGTT
     2461 GCTGGCCGTA CATTTGTACG GCTCCGCAGT GGATGGCGGC CTGAAGCCAC ACAGTGATAT
     2521 TGATTTGCTG GTTACGGTGA CCGTAAGGCT TGATGAAACA ACGCGGCGAG CTTTGATCAA
     2581 CGACCTTTTG GAAACTTCGG CTTCCCCTGG AGAGAGCGAG ATTCTCCGCG CTGTAGAAGT
     2641 CACCATTGTT GTGCACGACG ACATCATTCC GTGGCGTTAT CCAGCTAAGC GCGAACTGCA
     2701 ATTTGGAGAA TGGCAGCGCA ATGACATTCT TGCAGGTATC TTCGAGCCAG CCACGATCGA
     2761 CATTGATCTG GCTATCTTGC TGACAAAAGC AAGAGAACAT AGCGTTGCCT TGGTAGGTCC
     2821 AGCGGCGGAG GAACTCTTTG ATCCGGTTCC TGAACAGGAT CTATTTGAGG CGCTAAATGA
     2881 AACCTTAACG CTATGGAACT CGCCGCCCGA CTGGGCTGGC GATGAGCGAA ATGTAGTGCT
     2941 TACGTTGTCC CGCATTTGGT ACAGCGCAGT AACCGGCAAA ATCGCGCCGA AGGATGTCGC
     3001 TGCCGACTGG GCAATGGAGC GCCTGCCGGC CCAGTATCAG CCCGTCATAC TTGAAGCTAG
     3061 ACAGGCTTAT CTTGGACAAG AAGAAGATCG CTTGGCCTCG CGCGCAGATC AGTTGGAAGA
     3121 ATTTGTCCAC TACGTGAAAG GCGAGATCAC CAAGGTAGTC GGCAAATAAC CCTCGAGCCA
     3181 CCCATGACCA AAATCCCTTA ACGTGAGTTA CGCGTCGTTC CACTGAGCGT CAGACCCCGT
     3241 AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA
     3301 AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT
     3361 TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA
     3421 GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT
     3481 AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC
     3541 AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA
     3601 GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCATTGAGA
     3661 AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG
     3721 AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT
     3781 CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG
     3841 CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT
     3901 TGCTCACATG TT
//
