LOCUS       pC2HB                   9231 bp    DNA     circular UNA 19-MAR-1980
DEFINITION  LMBP 9241.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|pC2HB|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid

FEATURES             Location/Qualifiers
     CDS             321..2503
                     /vntifkey="4"
                     /label=CmLEU2
     misc_feature    2741..3854
                     /vntifkey="21"
                     /label=Integration\Sequence
     CDS             4499..5402
                     /vntifkey="4"
                     /label=kanamycin\resistance
     promoter        5563..7002
                     /vntifkey="29"
                     /label=MET3p
     misc_feature    7009..7044
                     /vntifkey="21"
                     /label=SV40\NLS
     CDS             7045..7662
                     /vntifkey="4"
                     /label=LexA
     linker          7669..7686
                     /vntifkey="21"
                     /label=glycine\linker
                     /note="Unknown feature type:linker"
     misc_marker     7687..7713
                     /vntifkey="22"
                     /label=HA\tag
     terminator      7787..8300
                     /vntifkey="43"
                     /label=CaACT1term
     rep_origin      8430..9103
                     /vntifkey="33"
                     /label=pUCori
BASE COUNT     2685 a      1852 c      1978 g      2716 t 
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat 
      241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca 
      301 ctagtaacgg ccgccagtgt gctggaattc gcccttggat ccaatcatca ctggtggggg 
      361 tcaagaaaga ggtttgagat caggtacttt ggcaccacca ttagtagctg ggtttggtga 
      421 agctgcaaga ttaatgaaac aagaatctgc atttgacaag aaacatattg agaggttatc 
      481 tactaaattg aagaacggtt tgttatccat tccatctact cagtttaacg gttgtaataa 
      541 cccaacatat caatacccag gttgtgtgaa tgtttccttt gcatacattg aaggggaatc 
      601 tttgttaatg gcattgaaag atattgcatt gagttctggt tctgcatgta cctctgcatc 
      661 tttggaacca tcgtatgttt tacatgcttt gggtgccgat gatgccttgg ctcattcttc 
      721 cattagattc ggtattggta gattcaccac tgaagcagaa gttgactatg ttattcaagc 
      781 aattaatgaa agagttgatt tcttgagaaa gatgtctcca ttatgggaaa tggttcaaga 
      841 aggtattgac ttgaactcta tcgaatggag tggacattaa ggttttttgt ttttttgtaa 
      901 tttgtgttgt tatgtgtaat tatcgtgtat agagtaattt cggaaattgt actttcgttc 
      961 atgtatctga accagatgaa taatacgttc gtattgttga agaatgacta actcctgtaa 
     1021 cggcgtgcgt gggttccgca gccgggttca attttttttt tcttgttcac tataaaaata 
     1081 tataataaac gaaaaaaatc cgagggactc aggctatgaa atttttcact caagaatttt 
     1141 ttttccttca tccttaattc ctttattcac tacccaaacc cacaatgtca gttaaaacta 
     1201 aaactattac aattttacca ggtgatcacg tcggtaccga aattgtcaat gaagctatca 
     1261 aagttttaga agcaattgaa gctgccactc cttaccaaaa aatccatttc gatttcaaac 
     1321 atcatttaat cggtggtgct gccattgatg ccactggtgt cccattgcca gatgacgcac 
     1381 ttgaaagtgc caaaaaatct gatgccgtct tattgggtgc tgttggtgga ccaaaatggg 
     1441 ggactggcgc cgttcgtcca gaacaaggtt tattaaagat tcgtaaagaa ttgaaccttt 
     1501 atgctaacat cagaccatgt aactttgcca gtgactcctt attggaatta tccccattga 
     1561 gaccagaagt cgttaaaggt acaaacttga tcattgttcg tgaattagtt ggtggtattt 
     1621 atttcggtga tcgtgaagaa caagaagaaa gtgaagataa acaaaccgca tgggatactg 
     1681 aaaaatacac cgtcgatgaa gtcaccagaa tcacccgtat ggctgccttc atggctttac 
     1741 aacataaccc accattacca atctggtcat tggataaggc taatgtttta gcttcttcta 
     1801 gattatggag aagaactgtc gataaagtga tttctgaaga gttcccaact ttgtctgttc 
     1861 aacatcaatt gattgactcc gccgccatga ttttaattca aaacccaacc aaattgaatg 
     1921 gtataatcat cacttctaac atgtttggtg atatcatttc cgatgaagct tcagttattc 
     1981 caggttcctt gggtttatta ccatctgcat ctttggcttc attgccagat accaacaccg 
     2041 cttttggtct ttatgaacca tgtcacggtt ctgctcctga tttaccagcc aataaagtta 
     2101 acccaattgc tactatttta tctgctgctt ctatgttgag attatctttg gattgtgtga 
     2161 aagaagctga agctttagaa gaagccgtga aacaagtctt ggataaaggt atcagaactg 
     2221 cagatttaag aggtaccagt tcagctactg aagttggtga cgcgattgtc gaagctgtta 
     2281 ctaaaatctt aaaagaaaaa gcttaagtaa agtatacaca aaatttaacg aatattatta 
     2341 atacaaaatg aacaccacct aaatactttt ttttaaaaat gtaccgactc ttctaaaaat 
     2401 tgcgataatt caatatcgga aatttctctc accggtttac ttggatcttc ggaaataaat 
     2461 ctatttgaag taatggtagc ctcacttgat tctgattggc tctttctaga catgggtagg 
     2521 taagggcgaa ttctgcagat atccatcaca ctggcggccg gccgctcgag catgcatcta 
     2581 gagggcccaa ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac 
     2641 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt 
     2701 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg accagatcaa aatgctagaa 
     2761 caaattacgt ctgcatcaac agtcatcttc cttatttgca taaatgctaa aatggcaaac 
     2821 ctgtaaaccg tttgaaaagc aaattgagat agttagctcg gttttttttt cacgtggcca 
     2881 taatccaaca aaaaaaagga atttaagggg ggagagaagg aggcttgaat ttgtaccaca 
     2941 gagcgagcaa gaaattaata atatacttgg cttagctgaa atgagaatga aaaagcaaat 
     3001 ttcaagtgaa acaccactac tagccaagta aattggccaa tttgctctcg tgctttaatt 
     3061 tcctaacaag cttttgagac cgaaaaaaat caaccaatga tgatggaaaa atacttccgg 
     3121 gagtcaattt atccataatt tcacgtcgtt ttttttttgc acatggcaaa tatattggaa 
     3181 tcctgagatt ttcttggagt cgtgtacaat aacaccaggc gggaacatgc cgttatgatc 
     3241 cagcatagcg gccgctatgt gttcaaatat tgaaaccgtg aaaccagttt gatacagctg 
     3301 atattgtgtg ctaaaaaaag atgagcaccg aaagagtctt tagtttattg ccaagtaaag 
     3361 ggtcaaagaa tacctattct ttatgaacac agacttgctc taatggtgaa ttagtgtgga 
     3421 gtaatagaga aggaggcgaa acaaaaataa ttggttaaac actcgaatgt cacacaatcc 
     3481 taaataggtt aaattcagtt accgccgtga tgcgggaaca aagccgagcg gtaatatttt 
     3541 tggattctat gtttttttgg tgtatattgc acgacttggt gccaacaact ggtgttccaa 
     3601 cgcacaaaac taggttcaac tggatactgt tttctagaaa ttatttgcgg caattcatga 
     3661 ttacggtcct aaatttgaaa taaaagtttc caattatgtg tcttgacaat ttccattggg 
     3721 ttcaaaaaga tctagaatgt atgtaattac gcaacaattt gcatgcaaag aaataaaaac 
     3781 aaacctaaaa tccaactgct gttgttctgg tgatgatgta gtaaaataag agcacggcca 
     3841 agaacaaggt gatttacgta cggcagttta aggtttacac ctataaaaga gagagccgtt 
     3901 atcgtctgtt tgtggatgta cagagtgata ttattgacac gccggggcga cggatggtga 
     3961 tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg 
     4021 tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct 
     4081 ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca 
     4141 ttaacctgat gttctgggga atataaatgt caggcatgag attatcaaaa aggatcttca 
     4201 cctagatcct tttcacgtag aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg 
     4261 tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag caggtagctt 
     4321 gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca agcgaaccgg 
     4381 aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg 
     4441 ctttcttgcc gccaaggatc tgatggcgca ggggatcaag ctctgatcaa gagacaggat 
     4501 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg 
     4561 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg 
     4621 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg 
     4681 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc 
     4741 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg 
     4801 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca 
     4861 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc 
     4921 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg 
     4981 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg 
     5041 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata 
     5101 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg 
     5161 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat 
     5221 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct 
     5281 tctatcgcct tcttgacgag ttcttctgaa ttattaacgc ttacaatttc ctgatgcggt 
     5341 attttctcct tacgcatctg tgcggtattt cacaccgcat caggtggcac ttttcgggga 
     5401 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc 
     5461 atgagacaat aaccctgata aatgcttcaa taatagcacg tgaggagggc caccatggcc 
     5521 aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcttccaatt agaggttgtg 
     5581 gatgcaaaat tgaaaaaaaa atgactattg gtagcgtgtt ctatttttct tatgcgctga 
     5641 aaaactacga acaattgtct attccaagcc tgtggctttt tttggtgatt cacaatacat 
     5701 aaattggcaa atacctcttc tatagacttt aacctttgat gaaatggtat ggataaacca 
     5761 cttttgtggt cagggtatat atatttttat tccaatctgg cctttataaa ttcaaatttc 
     5821 caaggggact ctgctaaacc ttctgatata actataactc taataatggc tgtaaaaagt 
     5881 gtgaaaaaaa gatgatattc acaaaaaaga taaaggggat ttcatattgg gtatctagat 
     5941 ttaaacatcg tatttcaaca atgaaactag aatcaatacg aaatccaggg cacaactgtg 
     6001 ttcattccct tgggaattat actgagtaat gggaagaatg tgcaaaaaat acccgtgtct 
     6061 tatataattt ccatttgtgt agggcttaaa agtggacaac ttcaggttac gaaaccacga 
     6121 aaatgtctaa acgtttgatc aaattcgtaa ctcatggaaa atcacagcaa caaattaagg 
     6181 atggatatat ccggtactta caaataaaaa cactaaagaa gtataaattt tgaactgaaa 
     6241 ttctatagaa aatctttttt tttgggtaaa attatgcgat tgtggctcat agtaacgtaa 
     6301 tcatacagtt caacaaacat cattttgtca aaggatttct aatcctattc tttttctact 
     6361 tggcttttga gtatatatat atatatatat acatcctttg ttcatcatca tgttcgattg 
     6421 aatttttttc ttggttgtta ttagattgtc tacttgagtt tttcttggtg caaattgtgg 
     6481 tgtttttcta acaaattttt caacatttaa agtatttctg gaacttttct acaaggaacg 
     6541 acatagatcc attaatgcgc ccctctaaaa caatacccat aatgtcttct taagtatgca 
     6601 tctgcttata aatgtttctg gatttacttt agttagaatg tcgacacgac aatgaactat 
     6661 tgctgtagat cacgtgcgat cactgaatta tgagtttctt tttatttttc tttttgggct 
     6721 tttttttttg caattttttt tttccacctt tgccaattgc gtagtcacat ataatgtata 
     6781 caatttttgc aactctgaca ctataattga tccaataata aagtatataa tagatcatca 
     6841 agtatacgta atctccccaa aaaaaaaatt gcataaaata aaatctataa acctttttta 
     6901 gatttttagt tgttcctttt ttctttctat tcttcaactt ccaagtgttg tcactttctt 
     6961 cctgttaact atatttaaaa gtaaataccc tccccagaaa actccggaat ggctgctcct 
     7021 ccaaaaaaga agagaaaggt agctagagaa ttaacaaaac gacaaagcga aatatataac 
     7081 tatattaaac aagttgttca aatgaaaggt tatccgccta gtgttcgcga aattggtgag 
     7141 gcagttggct tagcatccag ttcaactgtt catggtcacc tttcacgtct tgaagaaaaa 
     7201 ggctatataa gaagagatcc aacgaaacca cgtgctatag aaattgtaag tgatcaaaca 
     7261 aatgataata ttaatatgga agaaacgatt catgtgccag ttattggtaa agtcacagca 
     7321 ggtgttccta ttaccgcagt agaaaatatt gaagaatatt ttccattacc tgaacactta 
     7381 acatcgacac acaatagcga catattcata ttaaacgtcg taggcgacag tatgattgag 
     7441 gctggtatat tagacggaga caaagtaatt gttcgcagtc aaaccatagc agaaaatgga 
     7501 gacattattg ttgctatgac tgaggaagat gaagcaactg tcaaacgctt ctataaagaa 
     7561 aaaaatcgtt atcgattaca acctgaaaat agtacaatgg agccaattta cctcgacaat 
     7621 gttgctgtaa tcgggaaagt aattggtttg taccgcgaaa tgagatctgg tgctggtggt 
     7681 gttggttatc cgtatgatgt gcctgactac gcaggtgctg gtggtgttgg tgctagcagg 
     7741 cctttcgaaa ggcgcgccac gcgtagtact taaccgcgcc gtcgacgagt gaaattctgg 
     7801 aaatctggaa atctggtttt gtattcttgt tattcttctt tttgttatta catatataac 
     7861 ttgttacttt tttaaaaaaa tctttgttta ttttataaat atataaaact aaatttaaga 
     7921 aaaagagaaa aatgttttat ttgagagatt gaaattttac ttgaatttag cttagctttt 
     7981 ataaagtatt attatgtaaa aaaacaaaac aaatatacat taaaaagtta agactataaa 
     8041 atagccaccc aaggcatttc tatatcttgt tgttgttgtt ttcatcttct gtatcagagg 
     8101 aacttatttt attattttcg tcacgggtat tttctcttgt ttgatgattc atcccattca 
     8161 ttccatcata aaattctaga aggaccacct ttgattgtaa atagtaataa ttaccaccct 
     8221 tatttaatta tttatttaac ttatttattt atttattata catatataca aatctaataa 
     8281 agtgaaaatc tcccccttca cacgtggccg aggagcagga ctgacacgtg ctaaaacttc 
     8341 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc 
     8401 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 
     8461 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 
     8521 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 
     8581 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact 
     8641 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg 
     8701 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 
     8761 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 
     8821 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag 
     8881 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 
     8941 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac 
     9001 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca 
     9061 acgcggcctt tttacggttc ctgggctttt gctggccttt tgctcacatg ttctttcctg 
     9121 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc 
     9181 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa g 
//
