LOCUS       pCAXL-NB5.D9-escape        6170 bp    DNA     circular     28-JUL-2014
DEFINITION  LMBP 9323.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 9323
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 28-JUL-2014

COMMENT     VNTDATE|704730390|
COMMENT     VNTNAME|pCAXL-NB5.D9 escape|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 150 0 
COMMENT        1 0 (CHomObj 1 0 0 3 75) 
COMMENT        (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) (CWordArray) 
COMMENT        (CStringList "NheI" "NotI" "SacI" "XhoI" "MluI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "NheI" "gctagc" 2 0 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 1362 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "SacI" "gagctc" 6 0 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 5 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "MluI" "acgcgt" 2 0 1 1369 0 0 "")) 
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COMMENT        #6=(CFSignal (CObList) "3' flanking region of rabbit \337-globin" 21 0 0 
COMMENT            1552 1908 0 (CStringList) (CStringList) 1 1 1 1 "183..539") 
COMMENT        #7=(CFSignal (CObList) "lac promoter" 30 0 1 1986 2068 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(617..699)") 
COMMENT        #8=(CFSignal (CObList) "SV40 ori" 33 0 0 2210 2285 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "841..916") 
COMMENT        #9=(CFSignal (CObList) "SV40 polyA early" 43 0 0 2316 2390 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "947..1021") 
COMMENT        #10=(CFSignal (CObList) "SV40 polyA late" 43 0 1 2316 2390 0 
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COMMENT        #11=(CFSignal (CObList) "pMB1 ori" 33 0 1 2505 2906 0 (CStringList) 
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COMMENT        #12=(CFSignal (CObList) "ampicillin resistance" 4 0 1 3447 4307 0 
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COMMENT        #13=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 4443 4820 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3074..3451") 
COMMENT        #14=(CFSignal (CObList) "AG promoter" 29 0 0 4826 6167 0 (CStringList) 
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COMMENT        #15=(CFSignal (CObList) "intron AG promoter" 15 0 0 5195 6119 0 
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COMMENT                          "DNA Plasmid 'pCAXL-NB5.D9 escape'" 1) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                          "Created: 07/28/14 02:26" 1) 
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COMMENT                          "Last Modified: 07/28/14 02:26" 1) 
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COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT                          "Promoter Prokaryotic (1 total)") 
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FEATURES             Location/Qualifiers
     terminator      1460..1551
                     /vntifkey="43"
                     /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA
     misc_feature    1552..1908
                     /vntifkey="21"
                     /label=3'\flanking\region\of\rabbit\ß-globin
     promoter        complement(1986..2068)
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="E. coli lac operon promoter"
     rep_origin      2210..2285
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     terminator      2316..2390
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     terminator      complement(2316..2390)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     rep_origin      complement(2505..2906)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     CDS             complement(3447..4307)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        4443..4820
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
                     /note="human CMV-IE enhancer"
     promoter        4826..6167
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     intron          5195..6119
                     /vntifkey="15"
                     /label=intron\AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid intron"
     CDS             complement(10..1359)
                     /vntifkey="4"
                     /label=NA
BASE COUNT     1362 a      1625 c      1595 g      1588 t 
ORIGIN
        1 ttcctcgagc tacttgtcaa tggtgaatgg caactcagca ccgtctggcc aagaccaacc 
       61 cacagtgtca ctatttacac cacaaaaaga tatgctgctc ccactagtcc aagttgtgct 
      121 ctctttgggc cgccctctga tcaactcaac ccagaaacaa ggtcttatgc aatctagtcc 
      181 tgtcagctct ggatgctgga caaaactccc gctatatcct gaccaatcag ttattgctac 
      241 gatatcttgt ttcactgaaa agctgctgtc cgtttcagtc cacccatttg gatcccaaat 
      301 catttcgaag ccgctcctgg aattagtgct tttggttctc ccgatccaga caccattgcc 
      361 gtatttaaat gaaaaccctt ttaccccacc tgccccgtta gaggacaccg gaccacaact 
      421 acctgttcca tcattggggc gtggattgtc tccgaaaact ccactgcata tatatcctat 
      481 ttgatactcc aaattttgat tgaaagatac ccatggccga tttgaaccat gccaattatc 
      541 cctgcacaca catgtgattt cgccggcatc aggataacag gagcattcct catagtgata 
      601 attaggagca tccaattcga ctgatttaac caccttccct ttttccattt tgaagatctt 
      661 atgtgatgcc tgaccattac ttggtccgtc agtcattaca gtaaagcaag aaccatttac 
      721 acatgcacat tcagactctt gagttctcag tatgttgttc ctccaactct tgatagtgtc 
      781 tgttattatg ccattgtatt tcaatacagc caccgcccca ttgtctgggc cagaaattcc 
      841 aattgtcaac caactggtgc catcatggca agcacttgct gaccaagcaa cagactcaaa 
      901 ccttgagtta tatggggagg gagcctcacc cacaggacaa ctcattaatg ttctgtgagg 
      961 gcttctgtct ttgacagtcc cattggagtg cttgtcattc agcaaggctc cctgagtcaa 
     1021 aaagaaagtt ctgcattcca agtgggagca tgagatgaac ggctctctta taacaaacac 
     1081 atcccccttg gaaccgatcc ttatactgtt gtccttactg tatacagccc atccgttaat 
     1141 ggggcaaaga gatgaattgc ctgctaattt tactgaagcc acagctttct cagtaagcaa 
     1201 attagtattg ctgattggtt cagattggtg ttgattccct gtgtgaattg aatgactgac 
     1261 ccatattgag atcatgttcc caatttgtaa cattaagcta actattccag ttaccataca 
     1321 gattgatccg atggttatta tcttctgatt tggattcatg cggccgcacg cgttcgagga 
     1381 attcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct 
     1441 ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa 
     1501 gccccttgag catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg 
     1561 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca 
     1621 tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa 
     1681 caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt 
     1741 attccataga aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt 
     1801 tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct 
     1861 ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca 
     1921 gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca 
     1981 caattccaca caacatacga gccggaagca taaagtgtaa agcctgggtg cctaatgagt 
     2041 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 
     2101 gtgccagcgg atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc 
     2161 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 
     2221 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga 
     2281 ggcttttttg gaggcctagg cttttgcaaa aagctaactt gtttattgca gcttataatg 
     2341 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 
     2401 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgctgcatta 
     2461 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 
     2521 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 
     2581 ggcggtaata cggttatcca cagaatcagg gataacgcag gaaagaacat gtgagcaaaa 
     2641 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 
     2701 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 
     2761 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 
     2821 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 
     2881 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 
     2941 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 
     3001 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 
     3061 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 
     3121 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 
     3181 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 
     3241 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 
     3301 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 
     3361 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 
     3421 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 
     3481 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 
     3541 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 
     3601 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 
     3661 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 
     3721 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 
     3781 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 
     3841 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 
     3901 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 
     3961 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 
     4021 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 
     4081 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 
     4141 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 
     4201 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 
     4261 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 
     4321 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 
     4381 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggg 
     4441 tcgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag 
     4501 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc 
     4561 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg 
     4621 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca 
     4681 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc 
     4741 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt 
     4801 attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat 
     4861 ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc 
     4921 gatgggggcg gggggggggg gggggccccc cccaggcggg gcggggcggg gcgaggggcg 
     4981 gggcggggcg aggcggaaag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt 
     5041 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc 
     5101 gggagtcgtt gcgcgctgcc ttccccccgt gccccgctcc gccgccgcct cgcgccgccc 
     5161 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc 
     5221 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga 
     5281 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg 
     5341 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg 
     5401 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg 
     5461 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt 
     5521 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca 
     5581 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg 
     5641 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc 
     5701 ggggccgcct cgggccgggg agggctcggg ggaaggggcg cggcggcccc cggagcgccg 
     5761 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg 
     5821 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca 
     5881 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg 
     5941 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc 
     6001 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg 
     6061 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc 
     6121 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa 
//
