LOCUS       pT2R-P2A-Neo-BGH        4682 bp    DNA     circular     10-SEP-2014
DEFINITION  LMBP 9388.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|706979396|
COMMENT     VNTDBDATE|708706039|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pT2R-P2A-Neo-BGH|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|Pery Hackett|
COMMENT     VNTOAUTHOREML|perry@biosci.cbs.umn.edu|
COMMENT     VNTOAUTHORWWW|http://www.cbs.umn.edu/labs/perry|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pT2R-P2A-Neo-BGH" 0 4682 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 2 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "AarI" "AatII" "Acc65I" "AccI" "AceIII" "AciI" "AclI" 
COMMENT         "AcuI" "AfeI" "AflII" "AflIII" "AgeI" "AhdI" "AleI" "AloI" "AluI" 
COMMENT         "AlwI" "AlwNI" "ApaBI" "ApaI" "ApaLI" "ApoI" "AscI" "AseI" "AsiSI" 
COMMENT         "AvaI" "AvaII" "AvrII" "BaeI" "BamHI" "BanI" "BanII" "BbeI" "Bbr7I" 
COMMENT         "BbsI" "BbvCI" "BbvI" "BccI" "BceAI" "BcefI" "BcgI" "BciVI" "BclI" 
COMMENT         "BfaI" "BfrBI" "BglI" "BglII" "BlpI" "Bme1580I" "BmgBI" "BmrI" "BmtI" 
COMMENT         "BplI" "BpmI" "Bpu10I" "BpuEI" "BsaAI" "BsaBI" "BsaHI" "BsaI" "BsaJI" 
COMMENT         "BsaWI" "BsaXI" "BscAI" "BseMII" "BseRI" "BseYI" "BsgI" "BsiEI" 
COMMENT         "BsiHKAI" "BsiWI" "BslI" "BsmAI" "BsmBI" "BsmFI" "BsmI" "Bsp1286I" 
COMMENT         "Bsp24I" "BspCNI" "BspEI" "BspHI" "BspMI" "BsrBI" "BsrDI" "BsrFI" 
COMMENT         "BsrGI" "BsrI" "BssHII" "BssSI" "BstAPI" "BstBI" "BstEII" "BstF5I" 
COMMENT         "BstKTI" "BstNI" "BstUI" "BstXI" "BstYI" "BstZ17I" "Bsu36I" "BtgI" 
COMMENT         "BthCI" "BtsI" "Cac8I" "CdiI" "ChaI" "CjeI" "CjePI" "ClaI" "Csp6I" 
COMMENT         "CviAII" "CviJI" "DdeI" "DpnI" "DraI" "DraIII" "DrdI" "EaeI" "EagI" 
COMMENT         "EarI" "EciI" "Eco57MI" "EcoHI" "EcoICRI" "EcoNI" "EcoO109I" "EcoRI" 
COMMENT         "EcoRV" "EsaBC3I" "FalI" "FatI" "FauI" "FmuI" "Fnu4HI" "FokI" "FseI" 
COMMENT         "FspAI" "FspI" "GdiII" "HaeI" "HaeII" "HaeIII" "HaeIV" "HgaI" "HhaI" 
COMMENT         "Hin4I" "HincII" "HindIII" "HinfI" "HinP1I" "HpaI" "HpaII" "HphI" 
COMMENT         "Hpy188I" "Hpy188III" "Hpy8I" "Hpy99I" "HpyAV" "HpyCH4I" "HpyCH4III" 
COMMENT         "HpyCH4IV" "HpyCH4V" "HpyF10VI" "KasI" "KpnI" "LpnI" "MaeIII" "MboI" 
COMMENT         "MboII" "MfeI" "MluI" "MlyI" "MmeI" "MnlI" "MscI" "MseI" "MslI" 
COMMENT         "MspA1I" "MwoI" "NaeI" "NarI" "NciI" "NcoI" "NdeI" "NgoMIV" "NheI" 
COMMENT         "NlaIII" "NlaIV" "Nli3877I" "NotI" "NruI" "NsiI" "NspI" "PacI" "PciI" 
COMMENT         "PflMI" "PfoI" "PleI" "PmeI" "PmlI" "PpiI" "Ppu10I" "PpuMI" "PshAI" 
COMMENT         "PsiI" "Psp03I" "PspGI" "PspOMI" "PsrI" "PssI" "PstI" "PvuI" "PvuII" 
COMMENT         "RleAI" "RsaI" "RsrII" "SacI" "SacII" "SalI" "SanDI" "SapI" "Sau96I" 
COMMENT         "SbfI" "ScaI" "SciI" "ScrFI" "SelI" "SexAI" "SfaNI" "SfcI" "SfiI" 
COMMENT         "SfoI" "SgrAI" "SimI" "SmaI" "SmlI" "SnaBI" "SpeI" "SphI" "SrfI" 
COMMENT         "Sse232I" "Sse8647I" "SspD5I" "SspI" "Sth132I" "StsI" "StuI" "StyD4I" 
COMMENT         "StyI" "SwaI" "TaiI" "TaqI" "TaqII" "TatI" "TauI" "TfiI" "TseI" 
COMMENT         "Tsp45I" "Tsp509I" "TspDTI" "TspGWI" "TspRI" "Tth111I" "Tth111II" 
COMMENT         "UnbI" "UthSI" "VpaK11AI" "XbaI" "XcmI" "XhoI" "XmaI" "XmnI" "ZraI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 1 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 4682 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "AarI" "cacctgc" 12 16 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "AatII" "gacgtc" 6 0 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "Acc65I" "ggtacc" 2 0 1 1926 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "AccI" "gtmkac" 3 0 1 1906 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "AceIII" "cagctc" 14 18 3 2276 0 3516 0 4522 1 
COMMENT            0 "") 
COMMENT        #5=(CRSite (CStringList) "AciI" "ccgc" 2 4 51 426 1 430 0 466 1 535 1 
COMMENT            539 0 580 0 643 0 737 1 801 1 902 1 905 1 1145 0 1185 1 1190 0 1240 
COMMENT            1 1256 0 1282 0 1342 0 1568 1 2014 0 2117 0 2173 1 2183 1 2207 0 
COMMENT            2250 1 2257 1 2278 1 2369 0 2397 0 2524 0 2543 0 2664 0 2774 1 2909 
COMMENT            0 2918 1 3280 0 3371 0 3562 1 3608 0 3729 1 3773 0 3850 0 3959 0 
COMMENT            4058 1 4105 0 4148 1 4162 1 4165 1 4193 0 4220 0 4637 1 0 "") 
COMMENT        #6=(CRSite (CStringList) "AclI" "aacgtt" 3 0 2 3443 0 3816 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "AcuI" "ctgaag" 23 21 6 464 1 510 0 817 0 1249 
COMMENT            0 2872 0 3884 1 0 "") 
COMMENT        #8=(CRSite (CStringList) "AfeI" "agcgct" 4 0 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "AflII" "cttaag" 2 0 1 146 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "AflIII" "acrygt" 2 0 1 2324 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "AgeI" "accggt" 2 0 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "AhdI" "gacnnnnngtc" 7 0 1 3217 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "AleI" "cacnnnngtg" 6 0 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "AloI" "gaacnnnnnntcc" 26 21 1 4405 1 0 "") 
COMMENT        #15=(CRSite (CStringList) "AluI" "agct" 3 0 24 105 0 367 0 406 0 471 0 
COMMENT             777 0 1235 0 1586 0 1708 0 1899 0 1934 0 1989 0 2084 0 2148 0 2266 
COMMENT             0 2492 0 2582 0 2628 0 2885 0 3406 0 3506 0 3569 0 4279 0 4536 0 
COMMENT             4661 0 0 "") 
COMMENT        #16=(CRSite (CStringList) "AlwI" "ggatc" 10 11 16 104 1 117 0 850 0 915 
COMMENT             1 1096 0 1764 1 1884 1 1897 0 2885 1 2971 1 2973 0 3069 1 3070 0 
COMMENT             3533 1 3850 0 3854 1 0 "") 
COMMENT        #17=(CRSite (CStringList) "AlwNI" "cagnnnctg" 7 0 1 2740 0 0 "") 
COMMENT        #18=(CRSite (CStringList) "ApaBI" "gcannnnntgc" 9 0 0 0 "") 
COMMENT        #19=(CRSite (CStringList) "ApaI" "gggccc" 6 0 1 1924 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 2 2638 0 3884 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "ApoI" "raatty" 2 0 6 188 0 374 0 444 0 1793 0 
COMMENT             4549 0 4560 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "AseI" "attaat" 3 0 3 2095 0 2154 0 3389 0 0 
COMMENT             "") #24=(CRSite (CStringList) "AsiSI" "gcgatcgc" 6 0 0 0 "") 
COMMENT        #25=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 1 1911 0 0 "") 
COMMENT        #26=(CRSite (CStringList) "AvaII" "ggwcc" 2 0 4 522 0 1187 0 3355 0 3577 
COMMENT             0 0 "") #27=(CRSite (CStringList) "AvrII" "cctagg" 2 0 0 0 "") 
COMMENT        #28=(CRSite (CStringList) "BaeI" "acnnnngtayc" 24 19 0 0 "") 
COMMENT        #29=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 109 0 1889 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "BanI" "ggyrcc" 2 0 7 670 0 705 0 1424 0 1926 
COMMENT             0 2068 0 3165 0 4325 0 0 "") 
COMMENT        #31=(CRSite (CStringList) "BanII" "grgcyc" 6 0 5 107 0 1036 0 1577 0 
COMMENT             1924 0 4295 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "BbeI" "ggcgcc" 6 0 1 674 0 0 "") 
COMMENT        #33=(CRSite (CStringList) "Bbr7I" "gaagac" 14 18 1 1553 0 0 "") 
COMMENT        #34=(CRSite (CStringList) "BbsI" "gaagac" 9 13 1 1548 0 0 "") 
COMMENT        #35=(CRSite (CStringList) "BbvCI" "cctcagc" 3 6 0 0 "") 
COMMENT        #36=(CRSite (CStringList) "BbvI" "gcagc" 14 18 18 618 1 744 0 786 0 802 
COMMENT             1 895 1 1307 0 2135 1 2216 1 2234 1 2653 1 2743 0 2746 0 2952 0 
COMMENT             3255 1 3646 0 4196 0 4594 1 4667 1 0 "") 
COMMENT        #37=(CRSite (CStringList) "BccI" "ccatc" 10 11 11 425 0 448 1 549 1 891 
COMMENT             0 977 1 1387 0 3261 0 3385 0 3672 0 4351 1 4383 0 0 "") 
COMMENT        #38=(CRSite (CStringList) "BceAI" "acggc" 18 20 5 74 0 630 1 1097 0 2826 
COMMENT             0 4339 0 0 "") 
COMMENT        #39=(CRSite (CStringList) "BcefI" "acggc" 18 19 5 74 0 631 1 1097 0 2826 
COMMENT             0 4339 0 0 "") 
COMMENT        #40=(CRSite (CStringList) "BcgI" "cgannnnnntgc" 25 23 1 3756 0 0 "") 
COMMENT        #41=(CRSite (CStringList) "BciVI" "gtatcc" 13 12 3 890 0 2527 1 4054 1 0 
COMMENT             "") #42=(CRSite (CStringList) "BclI" "tgatca" 2 0 1 1346 0 0 "") 
COMMENT        #43=(CRSite (CStringList) "BfaI" "ctag" 2 0 11 407 0 421 0 439 0 1368 0 
COMMENT             1802 0 1896 0 1900 0 2819 0 3072 0 3407 0 4213 0 0 "") 
COMMENT        #44=(CRSite (CStringList) "BfrBI" "atgcat" 4 0 0 0 "") 
COMMENT        #45=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 2 3337 0 4604 0 0 "") 
COMMENT        #46=(CRSite (CStringList) "BglII" "agatct" 2 0 1 409 0 0 "") 
COMMENT        #47=(CRSite (CStringList) "BlpI" "gctnagc" 3 0 0 0 "") 
COMMENT        #48=(CRSite (CStringList) "Bme1580I" "gkgcmc" 6 0 5 617 0 710 0 1924 0 
COMMENT             2642 0 3888 0 0 "") 
COMMENT        #49=(CRSite (CStringList) "BmgBI" "cacgtc" 4 4 1 506 1 0 "") 
COMMENT        #50=(CRSite (CStringList) "BmrI" "actggg" 12 11 4 30 1 620 0 1691 0 3257 
COMMENT             1 0 "") #51=(CRSite (CStringList) "BmtI" "gctagc" 6 0 0 0 "") 
COMMENT        #52=(CRSite (CStringList) "BplI" "gagnnnnnctc" 25 0 1 1926 0 0 "") 
COMMENT        #53=(CRSite (CStringList) "BpmI" "ctggag" 23 21 2 520 0 3287 1 0 "") 
COMMENT        #54=(CRSite (CStringList) "Bpu10I" "cctnagc" 3 6 1 1852 1 0 "") 
COMMENT        #55=(CRSite (CStringList) "BpuEI" "cttgag" 23 21 5 1047 1 2415 1 2713 0 
COMMENT             2954 1 3822 1 0 "") 
COMMENT        #56=(CRSite (CStringList) "BsaAI" "yacgtr" 4 0 2 975 0 4366 0 0 "") 
COMMENT        #57=(CRSite (CStringList) "BsaBI" "gatnnnnatc" 6 0 0 0 "") 
COMMENT        #58=(CRSite (CStringList) "BsaHI" "grcgyc" 3 0 2 671 0 3754 0 0 "") 
COMMENT        #59=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 1 3278 1 0 "") 
COMMENT        #60=(CRSite (CStringList) "BsaJI" "ccnngg" 2 0 8 25 0 449 0 518 0 834 0 
COMMENT             1103 0 1416 0 2063 0 2484 0 0 "") 
COMMENT        #61=(CRSite (CStringList) "BsaWI" "wccggw" 2 0 4 702 0 2530 0 2677 0 
COMMENT             3508 0 0 "") 
COMMENT        #62=(CRSite (CStringList) "BsaXI" "acnnnnnctcc" 22 19 2 2169 1 4405 1 0 
COMMENT             "") 
COMMENT        #63=(CRSite (CStringList) "BscAI" "gcatc" 10 12 12 632 1 887 1 969 0 
COMMENT             1033 0 1103 1 1308 0 1474 0 1558 1 2420 0 3472 0 3667 1 3912 0 0 "") 
COMMENT        #64=(CRSite (CStringList) "BseMII" "ctcag" 16 14 5 1469 1 2613 0 3022 0 
COMMENT             3188 0 3705 1 0 "") 
COMMENT        #65=(CRSite (CStringList) "BseRI" "gaggag" 17 15 1 525 0 0 "") 
COMMENT        #66=(CRSite (CStringList) "BseYI" "cccagc" 2 6 4 1175 1 1559 1 1930 0 
COMMENT             2628 1 0 "") 
COMMENT        #67=(CRSite (CStringList) "BsgI" "gtgcag" 23 21 0 0 "") 
COMMENT        #68=(CRSite (CStringList) "BsiEI" "cgrycg" 5 0 8 430 0 539 0 580 0 2240 
COMMENT             0 2664 0 3587 0 3736 0 4632 0 0 "") 
COMMENT        #69=(CRSite (CStringList) "BsiHKAI" "gwgcwc" 6 0 6 107 0 784 0 974 0 
COMMENT             2642 0 3803 0 3888 0 0 "") 
COMMENT        #70=(CRSite (CStringList) "BsiWI" "cgtacg" 2 0 0 0 "") 
COMMENT        #71=(CRSite (CStringList) "BslI" "ccnnnnnnngg" 8 0 13 465 0 531 0 652 0 
COMMENT             1196 0 1788 0 1916 0 2172 0 2346 0 2364 0 2530 0 2809 0 4147 0 4473 
COMMENT             0 0 "") 
COMMENT        #72=(CRSite (CStringList) "BsmAI" "gtctc" 7 11 3 496 1 3278 1 4054 0 0 
COMMENT             "") #73=(CRSite (CStringList) "BsmBI" "cgtctc" 8 12 1 496 1 0 "") 
COMMENT        #74=(CRSite (CStringList) "BsmFI" "gggac" 16 20 1 822 0 0 "") 
COMMENT        #75=(CRSite (CStringList) "BsmI" "gaatgc" 8 6 0 0 "") 
COMMENT        #76=(CRSite (CStringList) "Bsp1286I" "gdgchc" 6 0 12 107 0 617 0 710 0 
COMMENT             784 0 974 0 1036 0 1577 0 1924 0 2642 0 3803 0 3888 0 4295 0 0 "") 
COMMENT        #77=(CRSite (CStringList) "Bsp24I" "gacnnnnnntgg" 25 20 2 2849 0 3027 0 
COMMENT             0 "") 
COMMENT        #78=(CRSite (CStringList) "BspCNI" "ctcag" 15 13 5 1470 1 2612 0 3021 0 
COMMENT             3187 0 3706 1 0 "") 
COMMENT        #79=(CRSite (CStringList) "BspEI" "tccgga" 2 0 0 0 "") 
COMMENT        #80=(CRSite (CStringList) "BspHI" "tcatga" 2 0 2 3044 0 4052 0 0 "") 
COMMENT        #81=(CRSite (CStringList) "BspMI" "acctgc" 11 15 2 558 1 939 0 0 "") 
COMMENT        #82=(CRSite (CStringList) "BsrBI" "ccgctc" 4 4 6 426 1 1284 0 2016 0 
COMMENT             2257 1 4058 1 4222 0 0 "") 
COMMENT        #83=(CRSite (CStringList) "BsrDI" "gcaatg" 9 7 4 904 0 1643 0 3278 0 
COMMENT             3452 1 0 "") 
COMMENT        #84=(CRSite (CStringList) "BsrFI" "rccggy" 2 0 4 990 0 1171 0 3297 0 
COMMENT             4261 0 0 "") #85=(CRSite (CStringList) "BsrGI" "tgtaca" 2 0 0 0 "") 
COMMENT        #86=(CRSite (CStringList) "BsrI" "actgg" 7 5 16 36 1 62 1 318 1 615 0 
COMMENT             816 0 1686 0 2124 1 2732 0 2745 0 2857 1 3263 1 3381 1 3424 1 3693 
COMMENT             0 3863 1 4455 0 0 "") 
COMMENT        #87=(CRSite (CStringList) "BssHII" "gcgcgc" 2 0 4 69 0 432 0 1068 0 1963 
COMMENT             0 0 "") 
COMMENT        #88=(CRSite (CStringList) "BssSI" "cacgag" 2 6 3 1263 1 2497 1 3881 1 0 
COMMENT             "") #89=(CRSite (CStringList) "BstAPI" "gcannnnntgc" 8 0 0 0 "") 
COMMENT        #90=(CRSite (CStringList) "BstBI" "ttcgaa" 3 0 0 0 "") 
COMMENT        #91=(CRSite (CStringList) "BstEII" "ggtnacc" 2 0 0 0 "") 
COMMENT        #92=(CRSite (CStringList) "BstF5I" "ggatg" 8 6 8 459 0 988 0 1013 0 1570 
COMMENT             0 3196 1 3377 1 3664 1 4680 0 0 "") 
COMMENT        #93=(CRSite (CStringList) "BstKTI" "gatc" 4 0 24 112 0 412 0 845 0 923 0 
COMMENT             1004 0 1013 0 1091 0 1349 0 1772 0 1892 0 2893 0 2968 0 2979 0 2987 
COMMENT             0 3065 0 3077 0 3182 0 3523 0 3541 0 3587 0 3845 0 3862 0 3898 0 
COMMENT             4632 0 0 "") 
COMMENT        #94=(CRSite (CStringList) "BstNI" "ccwgg" 3 0 8 26 0 520 0 1058 0 1418 0 
COMMENT             2064 0 2352 0 2473 0 2486 0 0 "") 
COMMENT        #95=(CRSite (CStringList) "BstUI" "cgcg" 3 0 19 71 0 73 0 432 0 434 0 
COMMENT             737 0 1038 0 1070 0 1965 0 2171 0 2173 0 2371 0 2952 0 3282 0 3775 
COMMENT             0 4107 0 4138 0 4162 0 4182 0 4558 0 0 "") 
COMMENT        #96=(CRSite (CStringList) "BstXI" "ccannnnnntgg" 9 0 0 0 "") 
COMMENT        #97=(CRSite (CStringList) "BstYI" "rgatcy" 2 0 11 109 0 409 0 842 0 1088 
COMMENT             0 1889 0 2965 0 2976 0 3062 0 3074 0 3842 0 3859 0 0 "") 
COMMENT        #98=(CRSite (CStringList) "BstZ17I" "gtatac" 4 0 0 0 "") 
COMMENT        #99=(CRSite (CStringList) "Bsu36I" "cctnagg" 3 0 0 0 "") 
COMMENT        #100=(CRSite (CStringList) "BtgI" "ccrygg" 2 0 2 449 0 1103 0 0 "") 
COMMENT        #101=(CRSite (CStringList) "BthCI" "gcngc" 5 0 38 1 0 429 0 432 0 538 0 
COMMENT              541 0 582 0 634 0 645 0 735 0 740 0 777 0 818 0 905 0 908 0 911 0 
COMMENT              1147 0 1243 0 1284 0 1298 0 2151 0 2232 0 2250 0 2253 0 2371 0 
COMMENT              2526 0 2669 0 2734 0 2737 0 2943 0 3271 0 3610 0 3637 0 3732 0 
COMMENT              3961 0 4151 0 4165 0 4187 0 4610 0 0 "") 
COMMENT        #102=(CRSite (CStringList) "BtsI" "gcagtg" 9 7 3 2110 1 3617 0 3637 1 0 
COMMENT              "") 
COMMENT        #103=(CRSite (CStringList) "Cac8I" "gcnngc" 4 0 31 71 0 434 0 486 0 496 
COMMENT              0 565 0 751 0 970 0 1036 0 1042 0 1070 0 1074 0 1115 0 1119 0 1173 
COMMENT              0 1376 0 1553 0 1557 0 1965 0 2117 0 2146 0 2255 0 2341 0 2378 0 
COMMENT              2938 0 3329 0 4206 0 4220 0 4263 0 4639 0 4659 0 4663 0 0 "") 
COMMENT        #104=(CRSite (CStringList) "CdiI" "catcg" 5 5 9 960 0 965 0 1109 1 1163 
COMMENT              0 1304 0 1470 0 3468 0 3869 1 4379 0 0 "") 
COMMENT        #105=(CRSite (CStringList) "ChaI" "gatc" 5 0 24 113 0 413 0 846 0 924 0 
COMMENT              1005 0 1014 0 1092 0 1350 0 1773 0 1893 0 2894 0 2969 0 2980 0 
COMMENT              2988 0 3066 0 3078 0 3183 0 3524 0 3542 0 3588 0 3846 0 3863 0 
COMMENT              3899 0 4633 0 0 "") 
COMMENT        #106=(CRSite (CStringList) "CjeI" "ccannnnnngt" 27 21 10 135 1 289 0 
COMMENT              1404 0 1956 0 2376 0 2817 1 2895 1 2931 0 2995 1 4645 1 0 "") 
COMMENT        #107=(CRSite (CStringList) "CjePI" "ccannnnnnntc" 27 21 6 482 1 2817 1 
COMMENT              2882 0 2995 1 3115 1 3587 1 0 "") 
COMMENT        #108=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") 
COMMENT        #109=(CRSite (CStringList) "Csp6I" "gtac" 2 0 3 976 0 1927 0 3696 0 0 "") 
COMMENT        #110=(CRSite (CStringList) "CviAII" "catg" 2 0 17 245 0 393 0 450 0 542 
COMMENT              0 887 0 1073 0 1104 0 1130 0 1556 0 1979 0 2325 0 3045 0 3536 0 
COMMENT              3546 0 3624 0 3660 0 4053 0 0 "") 
COMMENT        #111=(CRSite (CStringList) "CviJI" "rgcy" 3 0 87 61 0 105 0 367 0 397 0 
COMMENT              406 0 416 0 429 0 458 0 471 0 484 0 498 0 538 0 579 0 596 0 604 0 
COMMENT              631 0 656 0 740 0 749 0 753 0 777 0 815 0 891 0 908 0 927 0 990 0 
COMMENT              1034 0 1044 0 1061 0 1144 0 1171 0 1175 0 1212 0 1235 0 1251 0 
COMMENT              1352 0 1378 0 1575 0 1586 0 1597 0 1708 0 1851 0 1899 0 1922 0 
COMMENT              1934 0 1989 0 2041 0 2062 0 2084 0 2148 0 2165 0 2247 0 2266 0 
COMMENT              2339 0 2350 0 2368 0 2394 0 2492 0 2582 0 2628 0 2633 0 2658 0 
COMMENT              2737 0 2802 0 2813 0 2856 0 2885 0 3248 0 3260 0 3301 0 3327 0 
COMMENT              3331 0 3340 0 3406 0 3496 0 3506 0 3569 0 3607 0 4265 0 4279 0 
COMMENT              4293 0 4374 0 4516 0 4536 0 4607 0 4641 0 4661 0 0 "") 
COMMENT        #112=(CRSite (CStringList) "DdeI" "ctnag" 2 0 8 343 0 1478 0 1783 0 1852 
COMMENT              0 2599 0 3008 0 3174 0 3714 0 0 "") 
COMMENT        #113=(CRSite (CStringList) "DpnI" "gatc" 3 0 24 111 0 411 0 844 0 922 0 
COMMENT              1003 0 1012 0 1090 0 1348 0 1771 0 1891 0 2892 0 2967 0 2978 0 
COMMENT              2986 0 3064 0 3076 0 3181 0 3522 0 3540 0 3586 0 3844 0 3861 0 
COMMENT              3897 0 4631 0 0 "") 
COMMENT        #114=(CRSite (CStringList) "DraI" "tttaaa" 4 0 6 361 0 1631 0 1839 0 
COMMENT              3083 0 3102 0 3794 0 0 "") 
COMMENT        #115=(CRSite (CStringList) "DraIII" "cacnnngtg" 7 0 1 4369 0 0 "") 
COMMENT        #116=(CRSite (CStringList) "DrdI" "gacnnnnnngtc" 8 0 3 698 0 2432 0 4413 
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COMMENT        #272=(CRSite (CStringList) "ZraI" "gacgtc" 4 0 0 0 "")) 
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COMMENT                              (CGroupPar (CParagraph 54313504 (8 0) 1 1 1 0 178) 
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COMMENT                             "CCANNNNNNGTNNNNNNNNNNNNNNNN" 
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COMMENT                             "CCANNNNNNNTCNNNNNNNNNNNNNNN" 
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COMMENT                             "GGCGGANNNNNNNNNNNN" "CCGCCTNNNNNNNNNNNN") 
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COMMENT                             "NNNNNNNNNNNNNNNGAYNNNNNRTCNNNNNNNNNNNNNNN" 
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COMMENT                              (CGroupPar (CParagraph 55755136 (8 0) 1 1 1 0 178) 
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COMMENT                             "GACGCNNNNNNNNNNN" "CTGCGNNNNNNNNNNN") 
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COMMENT                              (CGroupPar (CParagraph 55755168 (8 0) 1 1 1 0 178) 
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COMMENT                              (CGroupPar (CParagraph 55755200 (8 0) 1 1 1 0 178) 
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COMMENT                             "GAYNNNNNVTCNNNNNNNNNNNNNN" 
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FEATURES             Location/Qualifiers
     CDS             1655..1882
                     /vntifkey="4"
                     /label=IR/DR(R)\Rmut13,Æ130,143,150
                     /note="IR/DR(R) has PCR induced mutations at bps 13 (T to A in the Ri), deleting bp 130 (ÆT), 143 (C to T) and 150 (C to A) [these bp designations correspond to the original defined IR/DR(R) from Ri to Ro, not the plasmid]"
     CDS             complement(119..345)
                     /vntifkey="4"
                     /label=IR/DR(L)\Lmut44
                     /note="IR/DR(L) appears to have a spontaneous mutation from A to T at bp 44 (happens to revert back to consensus) [the bp designation corresponds to the original defined IR/DR(L) from Lo to Li, not the plasmid]"
     promoter        74..94
                     /vntifkey="30"
                     /label=T7
     promoter        complement(1942..1961)
                     /vntifkey="30"
                     /label=T3
     promoter        complement(1986..2108)
                     /vntifkey="30"
                     /label=LacZ\promoter
     rep_origin      2202..3082
                     /vntifkey="33"
                     /label=ColE\Ori
     CDS             complement(3146..4003)
                     /vntifkey="4"
                     /label=Amp
     rep_origin      4134..4590
                     /vntifkey="33"
                     /label=F1\Ori
     modified_base   1883..1886
                     /vntifkey="23"
                     /label=TATA
     modified_base   115..118
                     /vntifkey="23"
                     /label=TATA
     polyA_signal    1358..1582
                     /vntifkey="25"
                     /label=BGH\polyA
     CDS             542..1336
                     /vntifkey="4"
                     /label=NeoR
     CDS             461..526
                     /vntifkey="4"
                     /label=P2A
BASE COUNT     1119 a      1163 c      1195 g      1205 t 
ORIGIN
        1 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg 
       61 ccagtgagcg cgcgtaatac gactcactat agggcgaatt ggagctcgga tccctataca 
      121 gttgaagtcg gaagtttaca tacacttaag ttggagtcat taaaactcgt ttttcaacta 
      181 ctccacaaat ttcttgttaa caaacaatag ttttggcaag tcagttagga catctacttt 
      241 gtgcatgaca caagtcattt ttccaacaat tgtttacaga cagattattt cacttataat 
      301 tcactgtatc acaattccag tgggtcagaa gtttacatac actaagttga ctgtgccttt 
      361 aaacagcttg gaaaattcca gaaaatgatg tcatggcttt agaagctaga tctggccatc 
      421 tagagcggcc gcgcgcacta gtgaattcca tggatggccc ggaagcggag ctactaactt 
      481 cagcctgctg aagcaggctg gagacgtgga ggagaaccct ggacctcaca gtggcggccg 
      541 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt 
      601 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc 
      661 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact 
      721 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt 
      781 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca 
      841 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat 
      901 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg 
      961 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga 
     1021 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga 
     1081 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa 
     1141 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga 
     1201 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt 
     1261 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct 
     1321 tgacgagttc ttctgaaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca 
     1381 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc 
     1441 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg 
     1501 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct 
     1561 ggggatgcgg tgggctctat ggaagcttgt ggaaggctac tcgaaatgtt tgacccaagt 
     1621 taaacaattt aaaggcaatg ctaccaaata ctaattgagt gtatgtaaac ttctgaccca 
     1681 ctgggaatgt gatgaaagaa ataaaagctg aaatgaatca ttctctctac tattattctg 
     1741 atatttcaca ttcttaaaat aaagtggtga tcctaactga cctaagacag ggaattttta 
     1801 ctaggattaa atgtcaggaa ttgtgaaaaa gtgagtttaa atgtatttgg ctaaggtgta 
     1861 tgtaaacttc cgacttcaac tgtataggga tcctctagct agagtcgacc tcgagggggg 
     1921 gcccggtacc cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat 
     1981 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 
     2041 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 
     2101 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 
     2161 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 
     2221 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 
     2281 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 
     2341 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 
     2401 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 
     2461 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 
     2521 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 
     2581 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 
     2641 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 
     2701 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 
     2761 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 
     2821 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 
     2881 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 
     2941 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 
     3001 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 
     3061 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 
     3121 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 
     3181 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 
     3241 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 
     3301 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 
     3361 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 
     3421 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 
     3481 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 
     3541 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 
     3601 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 
     3661 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 
     3721 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 
     3781 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 
     3841 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 
     3901 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 
     3961 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 
     4021 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 
     4081 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgcgc 
     4141 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 
     4201 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 
     4261 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt 
     4321 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc 
     4381 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct 
     4441 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga 
     4501 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 
     4561 attttaacaa aatattaacg cttacaattt ccattcgcca ttcaggctgc gcaactgttg 
     4621 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc 
     4681 tg 
//

