LOCUS       pCfB262                 6019 bp    DNA     circular     15-MAY-2020
DEFINITION  LMBP 9500.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 9500
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 15-MAY-2020
            NGS data implemented
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|728913614|
COMMENT     VNTDBDATE|-13342236|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCfB262|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCfB262" 0 6019 
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COMMENT        (CObList) -1 "") 
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COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
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COMMENT            4290 0 4746 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 2334 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "ScaI" "agtact" 4 0 2 649 0 3458 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 3700 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 1 768 0 0 "")) 
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COMMENT        #6=(CFSignal (CObList) "S. cerevisiae CYC1 terminator" 43 0 0 2129 2319 
COMMENT            0 (CStringList) (CStringList) 1 1 1 1 "2131..2321") 
COMMENT        #7=(CFSignal (CObList) "S. cerevisiae ADH1 terminator" 43 0 1 1970 2071 
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COMMENT        #8=(CFSignal (CObList) "S. cerevisiae chrom. XII fragment" 21 0 0 1414 
COMMENT            1867 0 (CStringList) (CStringList) 1 1 1 1 "1416..1869") 
COMMENT        #9=(CFSignal (CObList) "ampicillin resistance" 4 0 1 95 955 0 
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COMMENT        #10=(CFSignal (CObList) "loxP" 21 0 1 2367 2400 0 (CStringList) 
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COMMENT        #11=(CFSignal (CObList) "E. gossypii TEF1 promoter" 29 0 0 2420 2798 0 
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COMMENT        #12=(CFSignal (CObList) "S. pombe HIS5" 4 0 0 2799 3452 0 (CStringList) 
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COMMENT        #15=(CFSignal (CObList) "T1 terminator" 43 0 1 1259 1296 0 (CStringList) 
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COMMENT        #16=(CFSignal (CObList) "FRT" 21 0 0 1340 1373 0 (CStringList) 
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COMMENT        #20=(CFSignal (CObList) "T7 promoter" 30 0 1 4649 4663 0 (CStringList) 
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COMMENT                          "DNA Plasmid 'pCfB262'" 1) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 
COMMENT                          1) 
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COMMENT                          "Created: 03/20/15 12:00" 1) 
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COMMENT                          "length: 6019 bp" 1) 
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COMMENT                          "Modification Date in the Original DB: 20-MAR-2015" 1) 
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FEATURES             Location/Qualifiers
     misc_feature    3897..4572
                     /vntifkey="21"
                     /label=S.\cerevisiae\chrom.\XII\fragment
     terminator      2129..2319
                     /vntifkey="43"
                     /label=S.\cerevisiae\CYC1\terminator
     terminator      complement(1970..2071)
                     /vntifkey="43"
                     /label=S.\cerevisiae\ADH1\terminator
     misc_feature    1414..1867
                     /vntifkey="21"
                     /label=S.\cerevisiae\chrom.\XII\fragment
     CDS             complement(95..955)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     misc_feature    complement(2367..2400)
                     /vntifkey="21"
                     /label=loxP
     promoter        2420..2798
                     /vntifkey="29"
                     /label=E.\gossypii\TEF1\promoter
     CDS             2799..3452
                     /gene="HIS5"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="AAG34536.1"
                     /db_xref="GI:11344900"
                     /vntifkey="4"
                     /label=S.\pombe\HIS5
     terminator      3453..3687
                     /vntifkey="43"
                     /label=E.\gossypii\TEF1\terminator
     misc_feature    complement(3715..3748)
                     /vntifkey="21"
                     /label=loxP
     terminator      complement(1259..1296)
                     /vntifkey="43"
                     /label=T1\terminator
     misc_feature    1340..1373
                     /vntifkey="21"
                     /label=FRT
     misc_feature    complement(4615..4648)
                     /vntifkey="21"
                     /label=FRT
     misc_feature    2106..2129
                     /vntifkey="21"
                     /label=USER
     promoter        complement(3794..3810)
                     /vntifkey="30"
                     /label=T7\promoter
     promoter        complement(4649..4663)
                     /vntifkey="30"
                     /label=T7\promoter
     rep_origin      complement(5223..5622)
                     /vntifkey="33"
                     /label=pMB1\ori
BASE COUNT     1636 a      1331 c      1322 g      1730 t 
ORIGIN
        1 tctgacgctc agtggaacga cgcgcgcgta actcacgtta agggattttg gtcatgagct 
       61 tgcgccgtcc cgtcaagtca gcgtaatgct ctgcttacca atgcttaatc agtgaggcac 
      121 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 
      181 taactacgat acgggagggc ttaccatctg gccccagcgc tgcgatgata ccgcgagaac 
      241 cacgctcacc ggctccggat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 
      301 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 
      361 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccatcgct acaggcatcg 
      421 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 
      481 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 
      541 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 
      601 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 
      661 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 
      721 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 
      781 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 
      841 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 
      901 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatattct 
      961 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 
     1021 ttgaatgtat ttagaaaaat aaacaaatag gggtcagtgt tacaaccaat taaccaattc 
     1081 tgaacattat cgcgagccca tttatacctg aatatggctc ataacacccc ttgtttgcct 
     1141 ggcggcagta gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt 
     1201 agcgccgatg gtagtgtggg gactccccat gcgagagtag ggaactgcca ggcatcaaat 
     1261 aaaacgaaag gctcagtcga aagactgggc ctttcgcccg ggctaattat ggggtgtcgc 
     1321 ccttattcga ctctatagtg aagttcctat tctctagaaa gtataggaac ttctgaagtg 
     1381 gggatttaaa tgcggccgcg ctgagggttt aatcaatctg gcggcttgag ttctcaacat 
     1441 gttttatttt ttacttatat tgctggtagg gtaaaaaaat ataactccta ggaataggtt 
     1501 gtctatatgt ttttgtcttg cttctataat tgtaacaaac aaggaaaggg aaaatactgg 
     1561 gtgtaaaagc cattgagtca agttaggtca tcccttttat acaaaatttt tcaatttttt 
     1621 ttccaagatt cttgtacgat taattatttt ttttttgcgt cctacagcgt gatgaaaatt 
     1681 tcgcctgctg caagatgagc gggaacgggc gaaatgtgca cgcgcacaac ttacgaaacg 
     1741 cggatgagtc actgacagcc accgcagagg ttctgactcc tactgagctc tattggaggt 
     1801 ggcagaaccg gtaccggagg agaccgctat aaccggtttg aatttattgt cacagtgtca 
     1861 catcagcatt aagtcctcag cgagctcgca tggaatgcgt gcgatgagcg acctcatgct 
     1921 atacctgaga aagcaacctg acctacagga aagagttact caagaataag aattttcgtt 
     1981 ttaaaaccta agagtcactt taaaatttgt atacacttat tttttttata acttatttaa 
     2041 taataaaaat cataaatcat aagaaattcg cttatttaga agtgtcaaca acgtatctac 
     2101 caacggaatg cgtgcgatcg cgtgcattca tccgctctaa ccgaaaagga aggagttaga 
     2161 caacctgaag tctaggtccc tatttatttt tttatagtta tgttagtatt aagaacgtta 
     2221 tttatatttc aaatttttct tttttttctg tacagacgcg tgtacgcatg taacattata 
     2281 ctgaaaacct tgcttgagaa ggttttggga cgctcgaaga tcgcgtcagc tgaagcttcg 
     2341 tacgctgcag gtcgacaacc cttaatataa cttcgtataa tgtatgctat acgaagttat 
     2401 taggtctaga gatctgttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg 
     2461 gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac 
     2521 tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc catacatttt 
     2581 gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga 
     2641 aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata 
     2701 aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta 
     2761 ggatacagtt ctcacatcac atccgaacat aaacaaccat gggtaggagg gcttttgtag 
     2821 aaagaaatac gaacgaaacg aaaatcagcg ttgccatcgc tttggacaaa gctcccttac 
     2881 ctgaagagtc gaattttatt gatgaactta taacttccaa gcatgcaaac caaaagggag 
     2941 aacaagtaat ccaagtagac acgggaattg gattcctgga tcacatgtat catgcactgg 
     3001 ctaaacatgc aggctggagc ttacgacttt actcaagagg tgatttaatc atcgatgatc 
     3061 atcacactgc agaagatact gctattgcac ttggtattgc attcaagcag gctatgggta 
     3121 actttgccgg cgttaaaaga tttggacatg cttattgtcc acttgacgaa gccctttcta 
     3181 gaagcgtagt tgacttgtcg ggacggccct atgctgttat cgatttggga ttaaagcgtg 
     3241 aaaaggttgg ggaattgtcc tgtgaaatga tccctcactt actatattcc ttttcggtag 
     3301 cagctggaat tactttgcat gttacctgct tatatggtag taatgaccat catcgtgctg 
     3361 aaagcgcttt taaatctctg gctgttgcca tgcgcgcggc tactagtctt actggaagtt 
     3421 ctgaagtccc aagcacgaag ggagtgttgt aaagagtact gacaataaaa agattcttgt 
     3481 tttcaagaac ttgtcatttg tatagttttt ttatattgta gttgttctat tttaatcaaa 
     3541 tgttagcgtg atttatattt tttttcgcct cgacatcatc tgcccagatg cgaagttaag 
     3601 tgcgcagaaa gtaatatcat gcgtcaatcg tatgtgaatg ctggtcgcta tactgctgtc 
     3661 gattcgatac taacgccgcc atccagttta aacgagctct cgagaaccct taatataact 
     3721 tcgtataatg tatgctatac gaagttatta ggtgatatca gatccactag tggcctatgc 
     3781 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg 
     3841 tcgtgactgg gaaaaccctg gcgttacccc tgcaggacta gtgctgaggc attaatgcaa 
     3901 ctcagaagtt tgacagcaag caagttcatc attcgaacta gccttattgt tttagttcag 
     3961 tgacagcgaa ctgccgtact cgatgcttta tttctcacgg tagagcggaa gaacagatag 
     4021 gggcagcgtg agaagagtta gaaagtaaat ttttatcacg tctgaagtat tcttattcat 
     4081 aggaaatttt gcaaggtttt ttagctcaat aacgggctaa gttatataag gtgttcacgc 
     4141 gattttcttg ttatgtatac ctcttctctg aggaatggta ctactgtcct gatgtaggct 
     4201 ccttaaattg gtgggcaaga ataacttatc gatattttgt atattggtct tggagttcac 
     4261 cacgtaatgc ctgtttaaga ccatcagtta actctagtat tatttggtct tggctactgg 
     4321 ccgtttgcta ttattcaagt cttttgtgcc ttcccgtcgg gtaagggagt tatttaggga 
     4381 tacagaatct aacgaaaact aaatctcaat gattaactct atttaatcct tttttgaaag 
     4441 gcaaaagagg tcccttgttc acttacaacg ttcttagcca aattcgctta tcacttacta 
     4501 cttcacgata tacagaagta aaaacatata aaaagatgtc tgtttgttta gccatcacaa 
     4561 aaggtatcgc agattaagac ctcagcgcgg ccgcaaattt aaataaaatg aagtgaagtt 
     4621 cctatacttt ctagagaata ggaacttcta tagtgagtcg aataagggcg acacaaaatt 
     4681 tattctaaat gcataataaa tactgataac atcttatagt ttgtattata ttttgtatta 
     4741 tcgttgacat gtataatttt gatatcaaaa actgattttc cctttattat tttcgagatt 
     4801 tattttctta attctcttta acaaactaga aatattgtat atacaaaaaa tcataaataa 
     4861 tagatgaata gtttaattat aggtgttcat caatcgaaaa agcaacgtat cttatttaaa 
     4921 gtgcgttgct tttttctcat ttataaggtt aaataattct catatatcaa gcaaagtgac 
     4981 aggcgccctt aaatattctg acaaatgctc tttccctaaa ctccccccat aaaaaaaccc 
     5041 gccgaagcgg gtttttacgt tatttgcgga ttaacgatta ctcgttatca gaaccgccca 
     5101 gggggcccga gcttaagact ggccgtcgtt ttacaacaca gaaagagttt gtagaaacgc 
     5161 aaaaaggcca tccgtcaggg gccttctgct tagtttgatg cctggcagtt ccctactctc 
     5221 gccttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 
     5281 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 
     5341 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 
     5401 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 
     5461 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 
     5521 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 
     5581 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 
     5641 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 
     5701 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 
     5761 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 
     5821 ggctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 
     5881 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 
     5941 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 
     6001 tttgatcttt tctacgggg 
//
