LOCUS       pCfB391                 6065 bp    DNA     circular UNA  1-APR-2015
DEFINITION  LMBP 9505.
ACCESSION   BGHA URA3 ADH1 CYC1 Nb.BsmI
SOURCE      Saccharomyces cerevisiae (unknown).
  ORGANISM  Saccharomyces cerevisiae
            Saccharomyces cerevisiae Eukaryota; Fungi; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
COMMENT     Accession number: LMBP 9505
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 1-APR-2015
COMMENT     VNTDATE|728920037|
COMMENT     VNTNAME|pCfB391|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
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COMMENT       (CObList #0=(CRSite (CStringList) "BglII" "agatct" 2 0 1 2524 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "HaeII" "rgcgcy" 6 0 5 125 0 1110 0 3481 0 4935 
COMMENT            0 5540 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "SpeI" "actagt" 2 0 3 3516 0 3880 0 3991 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 3813 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "NsiI" "atgcat" 6 0 1 4642 0 0 "")) 
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COMMENT        #7=(CFSignal (CObList) "loxP" 21 0 1 2480 2513 0 (CStringList) 
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COMMENT        #8=(CFSignal (CObList) "loxP" 21 0 1 3828 3861 0 (CStringList) 
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COMMENT        #9=(CFSignal (CObList) "ampicillin resistance" 4 0 1 6064 859 0 
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COMMENT        #14=(CFSignal (CObList) "T7 promoter" 30 0 1 3907 3923 0 (CStringList) 
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COMMENT        #15=(CFSignal (CObList) "T7 promoter" 30 0 1 4597 4611 0 (CStringList) 
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COMMENT        #20=(CFSignal (CObList) "E. gossypii TEF1 promoter" 29 0 0 2568 2911 0 
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COMMENT        #21=(CFSignal (CObList) "E. gossypii TEF1 terminator" 43 0 0 3571 3768 0 
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COMMENT       (CObList) (CObList) (CObList) 
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COMMENT                          "DNA Plasmid 'pCfB391'" 1) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                          "Created: 03/20/15 01:47" 1) 
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COMMENT                          "Last Modified: 04/01/15 01:16" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6065 bp" 1) 
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COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1) 
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COMMENT                          "Division: UNA" 1) 
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COMMENT                          "Modification Date in the Original DB: 26-MAR-2015" 1) 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Accession Number(s): <a href=\"ncbilink:dnarna/BGHA\">BGHA</a>; <a href=\"ncbilink:dnarna/URA3\">URA3</a>; <a href=\"ncbilink:dnarna/ADH1\">ADH1</a>; <a href=\"ncbilink:dnarna/CYC1\">CYC1</a>; <a href=\"ncbilink:dnarna/Nb.BsmI\">Nb.BsmI</a>" 
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COMMENT                          "Sequence Source: Saccharomyces cerevisiae (unknown)." 
COMMENT                          1) 
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COMMENT                          "Organizm(s): <a href=\"ncbilink:taxonomy/Saccharomyces+cerevisiae\">Saccharomyces cerevisiae(Saccharomyces cerevisiae)</a>" 
COMMENT                          1) 
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COMMENT                          "Taxonomy: Saccharomyces cerevisiae Eukaryota; Fungi; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces" 
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COMMENT                                 "E. gossypii TEF1 promoter"))) 
COMMENT                          "Promoter Eukaryotic (1 total)") 
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COMMENT                           "Terminator (4 total)"))) "Feature Map") 
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FEATURES             Location/Qualifiers
     misc_feature    1318..1980
                     /vntifkey="21"
                     /label=S.\cerevisiae\chrom.\XI\integration\targeting\sequence
     terminator      complement(2083..2184)
                     /vntifkey="43"
                     /label=S.\cerevisiae\ADH1\terminator
     terminator      2242..2432
                     /vntifkey="43"
                     /label=S.\cerevisiae\CYC1\terminator
     misc_feature    4010..4521
                     /vntifkey="21"
                     /label=S.\cerevisiae\chrom.\XI\integration\targeting\sequence
     misc_feature    complement(2480..2513)
                     /vntifkey="21"
                     /label=loxP
     promoter        2568..2911
                     /vntifkey="29"
                     /label=E.\gossypii\TEF1\promoter
     terminator      3571..3768
                     /vntifkey="43"
                     /label=E.\gossypii\TEF1\terminator
     misc_feature    complement(3828..3861)
                     /vntifkey="21"
                     /label=loxP
     CDS             complement(6064..859)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(5171..5570)
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      complement(1163..1200)
                     /vntifkey="43"
                     /label=T1\terminator
     misc_feature    1244..1277
                     /vntifkey="21"
                     /label=FRT
     misc_feature    complement(4563..4596)
                     /vntifkey="21"
                     /label=FRT
     misc_feature    2214..2242
                     /vntifkey="21"
                     /label=USER\cloning\cassette
     promoter        complement(3907..3923)
                     /vntifkey="30"
                     /label=T7\promoter
     promoter        complement(4597..4611)
                     /vntifkey="30"
                     /label=T7\promoter
     CDS             2912..3565
                     /vntifkey="4"
                     /label=S.\pombe\HIS5
BASE COUNT     1677 a      1398 c      1294 g      1696 t 
ORIGIN
        1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 
       61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 
      121 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc 
      181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 
      241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 
      301 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 
      361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 
      421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 
      481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 
      541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 
      601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 
      661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 
      721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 
      781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 
      841 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt 
      901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca 
      961 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg 
     1021 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg 
     1081 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga 
     1141 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 
     1201 cccgggctaa ttatggggtg tcgcccttat tcgactctat agtgaagttc ctattctcta 
     1261 gaaagtatag gaacttctga agtggggatt taaatgcggc cgcgctgagg gtttaatgcg 
     1321 gagaagtcgt tgatagcatt tccgaaggct tttccacatt gactgcagta tctaaaagag 
     1381 ctccagcagc ggtcttagta gttcctgtgc ttgccgcttt gcagtataca gggacattgg 
     1441 gaactacgga ggaaaaatcg gaatgctctg agctcttctc aaaaccagac gaactcgtct 
     1501 tatttctcag taaattaccc aacatataaa ctctccctct tcaccttctc tacaaggcca 
     1561 cacacaagac agttttaaca ggcaaatggt actggaggct ctcaccaatg gggtttaaga 
     1621 cgctccaaac acacgctctc ctttttttct ttatatatcg gtagcaacga aagctagtcg 
     1681 cagaacgtta cacactaaag cagatcaatc aatcaatcct gagaacctgc ttcgttcaaa 
     1741 ccatccacta gaagaaacac gcacgtgtac ccaaaaatag agacaaacaa acagacaaaa 
     1801 ataacaatga ttcctaccga ctaaagtaaa acaacactca cgtaataaaa aatcctagat 
     1861 acaactcact tgtattcctc tcttctttga ccatcactaa agcttctctc ttactatatt 
     1921 cctcaaataa aaaaaagttg caatgattac ttaccaatgt gccataaact ccgtgcacca 
     1981 attaagtcct cagcgagctc gcatggaatg cgtgcgatga gcgacctcat gctatacctg 
     2041 agaaagcaac ctgacctaca ggaaagagtt actcaagaat aagaattttc gttttaaaac 
     2101 ctaagagtca ctttaaaatt tgtatacact tatttttttt ataacttatt taataataaa 
     2161 aatcataaat cataagaaat tcgcttattt agaagtgtca acaacgtatc taccaacgga 
     2221 atgcgtgcga tcgcgtgcat tcatccgctc taaccgaaaa ggaaggagtt agacaacctg 
     2281 aagtctaggt ccctatttat ttttttatag ttatgttagt attaagaacg ttatttatat 
     2341 ttcaaatttt tctttttttt ctgtacagac gcgtgtacgc atgtaacatt atactgaaaa 
     2401 ccttgcttga gaaggttttg ggacgctcga agatcgcgtc agctgaagct tcgtacgctg 
     2461 caggtcgaca acccttaata taacttcgta taatgtatgc tatacgaagt tattaggtct 
     2521 agagatctgt ttagcttgcc tcgtccccgc cgggtcaccc ggccagcgac atggaggccc 
     2581 agaataccct ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt gactgtcgcc 
     2641 cgtacattta gcccatacat ccccatgtat aatcatttgc atccatacat tttgatggcc 
     2701 gcacggcgcg aagcaaaaat tacggctcct cgctgcagac ctgcgagcag ggaaacgctc 
     2761 ccctcacaga cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat ataaaaggtt 
     2821 aggatttgcc actgaggttc ttctttcata tacttccttt taaaatcttg ctaggataca 
     2881 gttctcacat cacatccgaa cataaacaac catgggtagg agggcttttg tagaaagaaa 
     2941 tacgaacgaa acgaaaatca gcgttgccat cgctttggac aaagctccct tacctgaaga 
     3001 gtcgaatttt attgatgaac ttataacttc caagcatgca aaccaaaagg gagaacaagt 
     3061 aatccaagta gacacgggaa ttggattctt ggatcacatg tatcatgcac tggctaaaca 
     3121 tgcaggctgg agcttacgac tttactcaag aggtgattta atcatcgatg atcatcacac 
     3181 tgcagaagat actgctattg cacttggtat tgcattcaag caggctatgg gtaactttgc 
     3241 cggcgttaaa agatttggac atgcttattg tccacttgac gaagctcttt ctagaagcgt 
     3301 agttgacttg tcgggacggc cctatgctgt tatcgatttg ggattaaagc gtgaaaaggt 
     3361 tggggaattg tcctgtgaaa tgatccctca cttactatat tccttttcgg tagcagctgg 
     3421 aattactttg catgttacct gcttatatgg tagtaatgac catcatcgtg ctgaaagcgc 
     3481 ttttaaatct ctggctgttg ccatgcgcgc ggctactagt cttactggaa gttctgaagt 
     3541 cccaagcacg aagggagtgt tgtaaagagt actgacaata aaaagattct tgttttcaag 
     3601 aacttgtcat ttgtatagtt tttttatatt gtagttgttc tattttaatc aaatgttagc 
     3661 gtgatttata ttttttttcg cctcgacatc atctgcccag atgcgaagtt aagtgcgcag 
     3721 aaagtaatat catgcgtcaa tcgtatgtga atgctggtcg ctatactgct gtcgattcga 
     3781 tactaacgcc gccatccagt ttaaacgagc tctcgagaac ccttaatata acttcgtata 
     3841 atgtatgcta tacgaagtta ttaggtgata tcagatccac tagtggccta tgcacccaat 
     3901 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac 
     3961 tgggaaaacc ctggcgttac ccctgcagga ctagtgctga ggcattaatg ccttcgattt 
     4021 gacacatctc taagctgaaa ctgagaatac tgttgtaaaa caggtattgg ctgcttcata 
     4081 gtacacccaa ttgcccacaa cccaagttaa tcataagctc ttgttgtcga tgaattgctc 
     4141 aaaatgtggc cattctttca attttgcacc tctaaaactg ctccgtgggt tgtgacaaca 
     4201 accttaaggg taatggactt ttttagaaag acaaatgaga tgatttcacc tactggtata 
     4261 tcatttattt atacacgacc gttttgacat ttcagagttg cacgttggta tatcctaata 
     4321 ttctcacaag catcacagtg aagaaacgca agaaaatgga aaaaattccc aggtggttgt 
     4381 tgttttctct tatatcgtcg gtgctgtgca tacttggggc cctgtgtgtg ccgttgttat 
     4441 cggttgcctt cgatagcaag cgcaatagcc aatctaagtt ggtcaactat ggtctttctc 
     4501 taagtgccgg atctatgatc attaagacct cagcgcggcc gcaaatttaa ataaaatgaa 
     4561 gtgaagttcc tatactttct agagaatagg aacttctata gtgagtcgaa taagggcgac 
     4621 acaaaattta ttctaaatgc ataataaata ctgataacat cttatagttt gtattatatt 
     4681 ttgtattatc gttgacatgt ataattttga tatcaaaaac tgattttccc tttattattt 
     4741 tcgagattta ttttcttaat tctctttaac aaactagaaa tattgtatat acaaaaaatc 
     4801 ataaataata gatgaatagt ttaattatag gtgttcatca atcgaaaaag caacgtatct 
     4861 tatttaaagt gcgttgcttt tttctcattt ataaggttaa ataattctca tatatcaagc 
     4921 aaagtgacag gcgcccttaa atattctgac aaatgctctt tccctaaact ccccccataa 
     4981 aaaaacccgc cgaagcgggt ttttacgtta tttgcggatt aacgattact cgttatcaga 
     5041 accgcccagg gggcccgagc ttaagactgg ccgtcgtttt acaacacaga aagagtttgt 
     5101 agaaacgcaa aaaggccatc cgtcaggggc cttctgctta gtttgatgcc tggcagttcc 
     5161 ctactctcgc cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 
     5221 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 
     5281 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 
     5341 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 
     5401 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 
     5461 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 
     5521 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 
     5581 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 
     5641 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 
     5701 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 
     5761 aagtggtggg ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg 
     5821 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 
     5881 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 
     5941 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgacgc gcgcgtaact 
     6001 cacgttaagg gattttggtc atgagcttgc gccgtcccgt caagtcagcg taatgctctg 
     6061 ctttt 
//
