LOCUS       pCfB132                 5843 bp    DNA     circular     28-MAY-2019
DEFINITION  LMBP 9506.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 9506
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 28-MAY-2019
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|728923277|
COMMENT     VNTDBDATE|-12834281|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCfB132|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark-Zwijnaarde 71|
COMMENT     VNTOAUTHORAD3|B-9052 Gent Belgium|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTREPLTYPE|Phage
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTEXTCHREPL|Yeast
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        #3=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 3 1848 0 2207 0 3885 0 0 "")) 
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COMMENT        #7=(CFSignal (CObList) "S. cerevisiae ADH1 terminator" 43 0 1 1949 2050 
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COMMENT        #8=(CFSignal (CObList) "pMB1 ori" 33 0 1 2498 2900 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2498..2900)") 
COMMENT        #9=(CFSignal (CObList) "f1 ori" 33 0 1 1351 1806 0 (CStringList) 
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FEATURES             Location/Qualifiers
     CDS             417..1220
                     /vntifkey="4"
                     /label=S.\cerevisiae\URA3
     terminator      2250..2439
                     /vntifkey="43"
                     /label=S.\cerevisiae\CYC1\terminator
     CDS             complement(3441..4301)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     misc_feature    2194..2217
                     /vntifkey="21"
                     /label=USER\cloning\cassette
     terminator      complement(1949..2050)
                     /vntifkey="43"
                     /label=S.\cerevisiae\ADH1\terminator
     rep_origin      complement(2498..2900)
                     /vntifkey="33"
                     /label=pMB1\ori
     rep_origin      complement(1351..1806)
                     /vntifkey="33"
                     /label=f1\ori
     rep_origin      complement(4433..5413)
                     /vntifkey="33"
                     /label=2mu\ori
     terminator      complement(5491..5595)
                     /vntifkey="43"
                     /label=S.\cerevisiae\FLP\terminator
     misc_feature    complement(5396..5443)
                     /vntifkey="21"
                     /label=FRT
BASE COUNT     1647 a      1237 c      1341 g      1618 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc 
      241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg 
      301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc 
      361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt 
      421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat 
      481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca 
      541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg 
      601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg 
      661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca 
      721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac 
      781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa 
      841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg 
      901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct 
      961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac 
     1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg 
     1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa 
     1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa 
     1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac 
     1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac 
     1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat 
     1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa 
     1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca 
     1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg 
     1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta 
     1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg 
     1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa 
     1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg 
     1801 gcgcgtccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc 
     1861 ttcgctatta cgccagctga attggagcga cctcatgcta tacctgagaa agcaacctga 
     1921 cctacaggaa agagttactc aagaataaga attttcgttt taaaacctaa gagtcacttt 
     1981 aaaatttgta tacacttatt ttttttataa cttatttaat aataaaaatc ataaatcata 
     2041 agaaattcgc ttatttagaa gtgtcaacaa cgtatctacc aacgatttga cccttttcca 
     2101 tcttttcgta aatttctggc aaggtagaca agccgacaac cttgattgga gacttgacca 
     2161 aacctctggc gaagaattgt taattaagag ctcgaatgcg tgcgatcgcg tgcattcctc 
     2221 gagtaagctt ggtaccgcgg ctagctaaga tccgctctaa ccgaaaagga aggagttaga 
     2281 caacctgaag tctaggtccc tatttatttt tttatagtta tgttagtatt aagaacgtta 
     2341 tttatatttc aaatttttct tttttttctg tacagacgcg tgtacgcatg taacattata 
     2401 ctgaaaacct tgcttgagaa ggttttggga cgctcgaaga tccagctgca ttaatgaatc 
     2461 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 
     2521 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 
     2581 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 
     2641 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 
     2701 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 
     2761 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 
     2821 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 
     2881 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 
     2941 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 
     3001 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 
     3061 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 
     3121 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 
     3181 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 
     3241 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 
     3301 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 
     3361 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 
     3421 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 
     3481 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 
     3541 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 
     3601 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 
     3661 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 
     3721 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 
     3781 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 
     3841 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 
     3901 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 
     3961 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 
     4021 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 
     4081 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 
     4141 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 
     4201 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 
     4261 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 
     4321 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 
     4381 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgaacgaagc 
     4441 atctgtgctt cattttgtag aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa 
     4501 gaatctgagc tgcattttta cagaacagaa atgcaacgcg aaagcgctat tttaccaacg 
     4561 aagaatctgt gcttcatttt tgtaaaacaa aaatgcaacg cgagagcgct aatttttcaa 
     4621 acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgagagc gctattttac 
     4681 caacaaagaa tctatacttc ttttttgttc tacaaaaatg catcccgaga gcgctatttt 
     4741 tctaacaaag catcttagat tacttttttt ctcctttgtg cgctctataa tgcagtctct 
     4801 tgataacttt ttgcactgta ggtccgttaa ggttagaaga aggctacttt ggtgtctatt 
     4861 ttctcttcca taaaaaaagc ctgactccac ttcccgcgtt tactgattac tagcgaagct 
     4921 gcgggtgcat tttttcaaga taaaggcatc cccgattata ttctataccg atgtggattg 
     4981 cgcatacttt gtgaacagaa agtgatagcg ttgatgattc ttcattggtc agaaaattat 
     5041 gaacggtttc ttctattttg tctctatata ctacgtatag gaaatgttta cattttcgta 
     5101 ttgttttcga ttcactctat gaatagttct tactacaatt tttttgtcta aagagtaata 
     5161 ctagagataa acataaaaaa tgtagaggtc gagtttagat gcaagttcaa ggagcgaaag 
     5221 gtggatgggt aggttatata gggatatagc acagagatat atagcaaaga gatacttttg 
     5281 agcaatgttt gtggaagcgg tattcgcaat attttagtag ctcgttacag tccggtgcgt 
     5341 ttttggtttt ttgaaagtgc gtcttcagag cgcttttggt tttcaaaagc gctctgaagt 
     5401 tcctatactt tctagagaat aggaacttcg gaataggaac ttcaaagcgt ttccgaaaac 
     5461 gagcgcttcc gaaaatgcaa cgcgagctgc gcacatacag ctcactgttc acgtcgcacc 
     5521 tatatctgcg tgttgcctgt atatatatat acatgagaag aacggcatag tgcgtgttta 
     5581 tgcttaaatg cgtacttata tgcgtctatt tatgtaggat gaaaggtagt ctagtacctc 
     5641 ctgtgatatt atcccattcc atgcggggta tcgtatgctt ccttcagcac taccctttag 
     5701 ctgttctata tgctgccact cctcaattgg attagtctca tccttcaatg ctatcatttc 
     5761 ctttgatatt ggatcatact aagaaaccat tattatcatg acattaacct ataaaaatag 
     5821 gcgtatcacg aggccctttc gtc 
//
