LOCUS       pCfB1977                3832 bp    DNA     circular     20-MAR-2015
DEFINITION  LMBP 9511.
SOURCE      Cloning vector pUC19.
  ORGANISM  Cloning vector pUC19
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   New England Biolabs.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2007) Research Department, New England Biolabs,
            240 County Road, Ipswich, MA 01938, USA
COMMENT     Accession number: LMBP 9511
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 20-MAR-2015
            
COMMENT     VNTDATE|728930855|
COMMENT     VNTNAME|pCfB1977|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
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FEATURES             Location/Qualifiers
     gene            complement(2772..3632)
                     /gene="bla"
                     /vntifkey="60"
     promoter        complement(437..842)
                     /vntifkey="29"
                     /label=S.\cerevisiae\TEF1\promoter
     promoter        854..1551
                     /vntifkey="29"
                     /label=S.\cerevisiae\TDH3\promoter
     CDS             complement(2772..3632)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(1829..2231)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        complement(1654..1737)
                     /vntifkey="30"
                     /label=lac\promoter
     RBS             complement(1616..1653)
                     /vntifkey="32"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
BASE COUNT     1080 a       868 c       892 g       992 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccatgaca 
      421 gatttgtaat taaaacttag attagattgc tatgctttct ttctaatgag caagaagtaa 
      481 aaaaagttgt aatagaacaa gaaaaatgaa actgaaactt gagaaattga agaccgttta 
      541 ttaacttaaa tatcaatggg aggtcatcga aagagaaaaa aatcaaaaaa aaaaattttc 
      601 aagaaaaaga aacgtgataa aaatttttat tgcctttttc gacgaagaaa aagaaacgag 
      661 gcggtctctt ttttcttttc caaaccttta gtacgggtaa ttaacgacac cctagaggaa 
      721 gaaagagggg aaatttagta tgctgtgctt gggtgttttg aagtggtacg gcgatgcgcg 
      781 gagtccgaga aaatctggaa gagtaaaaaa ggagtagaaa cattttgaag ctatggtgtg 
      841 tgcatcagta gctataaaaa acacgctttt tcagttcgag tttatcatta tcaatactgc 
      901 catttcaaag aatacgtaaa taattaatag tagtgatttt cctaacttta tttagtcaaa 
      961 aaattagcct tttaattctg ctgtaacccg tacatgccca aaataggggg cgggttacac 
     1021 agaatatata acatcgtagg tgtctgggtg aacagtttat tcctggcatc cactaaatat 
     1081 aatggagccc gctttttaag ctggcatcca gaaaaaaaaa gaatcccagc accaaaatat 
     1141 tgttttcttc accaaccatc agttcatagg tccattctct tagcgcaact acagagaaca 
     1201 ggggcacaaa caggcaaaaa acgggcacaa cctcaatgga gtgatgcaac ctgcctggag 
     1261 taaatgatga cacaaggcaa ttgacccacg catgtatcta tctcattttc ttacaccttc 
     1321 tattaccttc tgctctctct gatttggaaa aagctgaaaa aaaaggttga aaccagttcc 
     1381 ctgaaattat tcccctactt gactaataag tatataaaga cggtaggtat tgattgtaat 
     1441 tctgtaaatc tatttcttaa acttcttaaa ttctactttt atagttagtc ttttttttag 
     1501 ttttaaaaca ccaagaactt agtttcgaat aaacacacat aaacaaacaa aagtgcaggt 
     1561 ggggatcctc tagagtcgac ctgcaggcat gcaagcttgg cgtaatcatg gtcatagctg 
     1621 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 
     1681 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 
     1741 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc 
     1801 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg 
     1861 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 
     1921 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 
     1981 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 
     2041 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 
     2101 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 
     2161 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 
     2221 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 
     2281 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 
     2341 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 
     2401 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta 
     2461 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 
     2521 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 
     2581 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 
     2641 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 
     2701 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 
     2761 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 
     2821 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 
     2881 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 
     2941 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 
     3001 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 
     3061 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 
     3121 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 
     3181 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 
     3241 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 
     3301 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 
     3361 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 
     3421 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 
     3481 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 
     3541 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 
     3601 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 
     3661 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 
     3721 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt 
     3781 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tc 
//
