LOCUS       Col1a1-5'pEntry         3867 bp    DNA     circular UNA  9-MAR-2015
DEFINITION  LMBP 9521.
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com/
COMMENT     VNTDATE|727960308|
COMMENT     VNTNAME|Col1a1 5'pEntry|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "Col1a1 5'pEntry" 0 3867 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 0 0 1 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 3867 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #0=(CFSignal (CObList) 
COMMENT            "chicken \337-globin 5'HS4 insulator core sequence" 21 0 0 537 779 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "537..779") 
COMMENT        #1=(CFSignal (CObList) 
COMMENT            "chicken \337-globin 5'HS4 insulator core sequence" 21 0 0 780 1022 
COMMENT            0 (CStringList) (CStringList) 1 1 1 1 "780..1022") 
COMMENT        #2=(CFSignal (CObList) "Tn903 neomycin resistance" 4 0 1 2852 3667 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #3=(CFSignal (CObList) "attL4" 0 0 0 433 528 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #4=(CFSignal (CObList) "attR1" 0 0 1 1482 1606 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #5=(CFSignal (CObList) "hCMVT promoter" 29 0 0 1037 1400 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #6=(CFSignal (CObList) "pMB1 ori" 33 0 1 1909 2311 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #7=(CFSignal (CObList) "lac promoter" 30 0 1 1734 1817 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList) (CObList) 
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COMMENT            (CObjectList 
COMMENT             #9=(CRefLinePar 
COMMENT                 (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Col1a1 5'pEntry" 2) 
COMMENT                 5 "" 0 4) 
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COMMENT                  (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                   (CObjectList 
COMMENT                    #11=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "DNA 'Col1a1 5'pEntry'" 1) 
COMMENT                    #12=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                    #13=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "Created: 03/09/15 11:11" 1) 
COMMENT                    #14=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "Last Modified: 03/09/15 11:11" 1) 
COMMENT                    #15=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "length: 3867 bp" 1) 
COMMENT                    #16=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "storage type: Basic" 1) 
COMMENT                    #17=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "form: Circular" 1))) "General Description") 
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COMMENT                   (CObjectList 
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COMMENT                         "Original Source Database: GenBank" 1) 
COMMENT                    #20=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "Division: UNA" 1) 
COMMENT                    #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "Modification Date in the Original DB: 22-OCT-2010" 1))) 
COMMENT                  "Standard Fields") 
COMMENT             #22=(CRefLinePar 
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COMMENT                  0 0) 
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COMMENT                  "Annotations") 
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COMMENT                                 (CObjectList 
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COMMENT                                       "Start: 433   End: 528 " 1))) "attL4") 
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COMMENT                                       "Start: 2852  End: 3667 (Complementary)" 
COMMENT                                       1))) "Tn903 neomycin resistance"))) 
COMMENT                         "CDS (1 total)") 
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COMMENT                                       "Original Location Description:" 1) 
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COMMENT                                       "   537..779" 1))) 
COMMENT                                "chicken \337-globin 5'HS4 insulator core sequence") 
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COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #41=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "   780..1022" 1))) 
COMMENT                                "chicken \337-globin 5'HS4 insulator core sequence"))) 
COMMENT                         "Misc. Feature (2 total)") 
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COMMENT                          (CObjectList 
COMMENT                           #43=(CFolderPar 
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COMMENT                                 (CObjectList 
COMMENT                                  #44=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Start: 1037  End: 1400" 1))) 
COMMENT                                "hCMVT promoter"))) 
COMMENT                         "Promoter Eukaryotic (1 total)") 
COMMENT                    #45=(CFolderPar 
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COMMENT                          (CObjectList 
COMMENT                           #46=(CFolderPar 
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COMMENT                                 (CObjectList 
COMMENT                                  #47=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Start: 1734  End: 1817 (Complementary)" 
COMMENT                                       1))) "lac promoter"))) 
COMMENT                         "Promoter Prokaryotic (1 total)") 
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COMMENT                         "Replication Origin (1 total)"))) "Feature Map") 
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COMMENT                         "   Similarity between ambiguous nucleotides (if any)" 
COMMENT                         1) 
COMMENT                    #53=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "is calculated as an AVERAGE similarity" 1))) 
COMMENT                  "Motifs (both strands)")))) 
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COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    0.324447 1.64819 1 0.082322) 0.8 1.8 1) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "Tn903 neomycin resistance" "@N" 1 -16.2216 
COMMENT                                   6.96932 0 -10 11.0817 1.16519 #126#)) 
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COMMENT                      #128=(CGroupWidget 
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COMMENT                                    100) (LOGPEN 0 6 16711680) 8 0.835356 
COMMENT                                   5.00946 5.40367) 
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COMMENT                                   "chicken \337-globin 5'HS4 insulator core sequence" 
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FEATURES             Location/Qualifiers
     misc_feature    537..779
                     /vntifkey="21"
                     /label=chicken\ß-globin\5'HS4\insulator\core\sequence
     misc_feature    780..1022
                     /vntifkey="21"
                     /label=chicken\ß-globin\5'HS4\insulator\core\sequence
     CDS             complement(2852..3667)
                     /vntifkey="4"
                     /label=Tn903\neomycin\resistance
     attenuator      433..528
                     /vntifkey="0"
                     /label=attL4
     attenuator      complement(1482..1606)
                     /vntifkey="0"
                     /label=attR1
     promoter        1037..1400
                     /vntifkey="29"
                     /label=hCMVT\promoter
     rep_origin      complement(1909..2311)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        complement(1734..1817)
                     /vntifkey="30"
                     /label=lac\promoter
BASE COUNT     1027 a       972 c       964 g       904 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac 
      421 gaattcagta ctaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa 
      481 ttgataagca atgctttttt ataatgccaa ctttgtatag aaaagttggc ggccgcgagc 
      541 tcacggggac agcccccccc caaagccccc agggatgtaa ttacgtccct cccccgctag 
      601 ggggcagcag cgagccgccc ggggctccgc tccggtccgg cgctcccccc gcatccccga 
      661 gccggcagcg tgcggggaca gcccgggcac ggggaaggtg gcacgggatc gctttcctct 
      721 gaacgcttct cgctgctctt tgagcctgca gacacctggg ggatacgggg aaaaagcttg 
      781 agctcacggg gacagccccc ccccaaagcc cccagggatg taattacgtc cctcccccgc 
      841 tagggggcag cagcgagccg cccggggctc cgctccggtc cggcgctccc cccgcatccc 
      901 cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg atcgctttcc 
      961 tctgaacgct tctcgctgct ctttgagcct gcagacacct gggggatacg gggaaaaagc 
     1021 ttgcggccgc gatatcttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt 
     1081 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg 
     1141 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt 
     1201 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta 
     1261 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt 
     1321 gaaagtcgag ctcggtaccc gggtcgaggt aggcgtgtac ggtgggaggc ctatataagc 
     1381 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc 
     1441 catagaagac accgggaccg atccagcctc cgcggcgata tccaagtttg tacaaaaaag 
     1501 ttgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa 
     1561 aacagactac ataatactgt aaaacacaac atatgcagtc actatgaatc aactacttag 
     1621 atggtattag tgacctgtaa gtactggatc cgttccatcc caatggcgcg ccgagcttgg 
     1681 ctcgagcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 
     1741 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca 
     1801 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 
     1861 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 
     1921 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 
     1981 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 
     2041 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 
     2101 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 
     2161 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 
     2221 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 
     2281 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 
     2341 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 
     2401 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 
     2461 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 
     2521 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 
     2581 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 
     2641 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 
     2701 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 
     2761 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 
     2821 aaagtatata tgagtaaact tggtctgaca gttagaaaaa ctcatcgagc atcaaatgaa 
     2881 actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta 
     2941 atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg 
     3001 cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt 
     3061 tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagtttat 
     3121 gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg 
     3181 catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc 
     3241 tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg 
     3301 catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc 
     3361 cagggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg 
     3421 tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat 
     3481 tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca 
     3541 atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata 
     3601 aatcagcatc catgttggaa tttaatcgcg gcctagagca agacgtttcc cgttgaatat 
     3661 ggctcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 
     3721 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 
     3781 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa 
     3841 ataggcgtat cacgaggccc tttcgtc 
//
