LOCUS       pAZILcos                8194 bp    DNA     circular      4-FEB-2016
DEFINITION  LMBP 9597.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 9597
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 4-FEB-2016
COMMENT     VNTDATE|741285581|
COMMENT     VNTNAME|pAZILcos|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT        (CStringList "AgeI" "HincII" "HindIII" "PvuI" "SacII") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
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COMMENT        #2=(CRSite (CStringList) "HincII" "gtyrac" 4 0 5 940 0 2956 0 4881 0 
COMMENT            5757 0 8156 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "HindIII" "aagctt" 2 0 2 956 0 3038 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 1 1082 0 0 "")) 
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COMMENT        #9=(CFSignal (CObList) "erythromycin resistance" 4 0 1 5629 6366 0 
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COMMENT                          "DNA Plasmid 'pAZILcos'" 1) 
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COMMENT                          "Created: 08/03/15 04:39" 1) 
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COMMENT              (LOGPEN 0 0 0) 1.79389e-290 1.79394e-290) 
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COMMENT              3.17913e-316 2.5209e-071))))
FEATURES             Location/Qualifiers
     CDS             890..1396
                     /vntifkey="4"
                     /label=lacZ
     CDS             complement(2555..4045)
                     /vntifkey="4"
                     /label=repE
     CDS             complement(4088..4387)
                     /vntifkey="4"
                     /label=repD
     CDS             complement(4754..4897)
                     /vntifkey="4"
                     /label=copS\like
     CDS             complement(5629..6366)
                     /vntifkey="4"
                     /label=erythromycin\resistance
     rep_origin      complement(8182..428)
                     /vntifkey="33"
                     /label=p15A\ori
     CDS             complement(5038..5508)
                     /vntifkey="4"
                     /label=transposase\Tn3
     rep_origin      complement(2060..4974)
                     /vntifkey="33"
                     /label=pAMB1\ori
     misc_feature    complement(7103..8181)
                     /vntifkey="21"
                     /label=cos
BASE COUNT     2231 a      1791 c      1532 g      2640 t 
ORIGIN
        1 gtttttccat aggctccgcc cccctgacaa gcatcacgaa atctgacgct caaatcagtg 
       61 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggcg gctccctcgt 
      121 gcgctctcct gttcctgcct ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt 
      181 gtctcattcc acgcctgaca ctcagttccg ggtaggcagt tcgctccaag ctggactgta 
      241 tgcacgaacc ccccgttcag tccgaccgct gcgccttatc cggtaactat cgtcttgagt 
      301 ccaacccgga aagacatgca aaagcaccac tggcagcagc cactggtaat tgatttagag 
      361 gagttagtct tgaagtcatg cgccggttaa ggctaaactg aaaggacaag ttttggtgac 
      421 tgcgctcctc caagccagtt acctcggttc aaagagttgg tagctcagag aaccttcgaa 
      481 aaaccgccct gcaaggcggt tttttcgttt tcagagcaag agattacgcg cagaccaaaa 
      541 cgatctcaag aagatcatct tattaatcag ataaaatatt tctagatttc agtgcaattt 
      601 atctcttcaa atgtagcacc tgaagtcagc cccatacgat ataagttgta attctcatgt 
      661 ttgacagctt atcatcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat 
      721 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt 
      781 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt 
      841 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat 
      901 tacgaattcg agctcggtac ccggggatcc tctagagtcg acctgcaggc atgcaagctt 
      961 ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa 
     1021 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga 
     1081 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc 
     1141 accagaagcg gtgccggaaa gctggctgga gtgcgatctt cctgaggccg atactgtcgt 
     1201 cgtcccctca aactggcaga tgcacggtta cgatgcgccc atctacacca acgtgaccta 
     1261 tcccattacg gtcaatccgc cgtttgttcc cacggagaat ccgacgggtt gttactcgct 
     1321 cacatttaat gttgatgaaa gctggctaca ggaaggccag acgcgaatta tttttgatgg 
     1381 cgttcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaatgcgaa ttttaacaaa 
     1441 ataacgccat gagcggcctc atttcttatt ctgagttaca acagtccgca ccgctgccgg 
     1501 tagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt 
     1561 tatcatgcaa ctcgtaggac aggtgccggc agcgcccaac agtcccccgg ccacggggcc 
     1621 tgccaccata cccacgccga aacaagcgcc ctgcaccatt atgttccgga tctgcatcgc 
     1681 agatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctaaccgttt 
     1741 ttatcagctc tgggaggcag aataaatgat catatcgtca attattacct ccacggggag 
     1801 agcctgagca aactggcctc aggcatttga gaagcacacg gtcacactgc ttccggtagt 
     1861 caataaaccg gtaaaccagc aatagacata agcggctatt taacgaccct gccctgaacc 
     1921 gacgaccggg tcgaatttgc tttcgaattt ctgccattca tccgcttatt atcacttatt 
     1981 caggcgtagc aaccaggcgt ttaagggcac caataactgc cttaaaaaaa ttacgccccg 
     2041 ccctgccact catcgcagta attctattta atcactttga ctagcaaata ctaacaacaa 
     2101 gacacacaca ccaaaaatca aaaattcact acttttagtt aaaaaccacg taaccacaag 
     2161 aactaatcca atccatgtaa tcgggttctt caaatatttc tccaagattt tcctcctcta 
     2221 atatgctcaa cttaaatgac ctattcaata aatctattat gctgctaaat agtttatagg 
     2281 acaaataagt atactctaat gacctataaa agatagaaaa ttaaaaaatc aagtgttcgc 
     2341 ttcgctctca ctgcccctcg acgttttagt agcctttccc tcacttcgtt cagtccaagc 
     2401 caactaaaag ttttcgggct actctctcct tctcccccta ataattaatt aaaatcttac 
     2461 tctgtatatt tctgctaatc attcgctaaa cagcaaagaa aaaacaaaca cgtatcatag 
     2521 atataaatgt aatggcatag tgcgggtttt attttcagcc tgtatcatag ctaaacaaat 
     2581 cgagttgtgt gtccgtttta gggcgttctg ctagcttgtt taaagtctct tgaatgaatg 
     2641 tatgctctaa gtcaaaagaa tttgtcagcg cctttatata gctttctttt tcttcttttt 
     2701 ttactttaat gatcgatagc aacaatgatt taacactagc aagttgaatg ccaccatttc 
     2761 ttcctggttt aatcttaaag aaaatttcct gattcgcctt cagtaccttc agcaatttat 
     2821 ctaatgtccg ttcaggaatg cctagcactt ctctaatctc ttttttggtc gtcactaaat 
     2881 aaggcttgta tacatcgctt ttttcgctaa tataagccat taaatcttct ttccattctg 
     2941 acaaatgaac acgttgacgt tcgcttcttt ttttcttgaa tttaaaccac ccttgacgga 
     3001 caaataaatc tttactggtt aaatcacttg atacccaagc tttgcaaaga atggtaatgt 
     3061 attccctatt agccccttga tagttttctg aataggcact tctaacaatt ttgattactt 
     3121 ctttttcttc taagggttga tctaatcgat tattaaactc aaacatatta tattcgcacg 
     3181 tttcgattga atagcctgaa ctaaagtagg ctaaagagag ggtaaacatg acgttattac 
     3241 gccctattaa acccttttct cctgaaaatt tcgtttcgtg caataagaga ttaaaccagg 
     3301 gttcatctac ttgttttttg ccttctgtac cgcttaaaac cgttagactt gaacgagtaa 
     3361 agcccttatt atctgtttgt ttgaaagacc aatcttgcca ttctttgaaa gaataacggt 
     3421 aattaggatc aaaaaattct acattgtccg ttcttggtat gcgagcaata ccaaaatgat 
     3481 tacacgttag atcaactggc aaagactttc caaaatattc tcggatattt tgcgaaatta 
     3541 ttttggctgc tttgacagat ttaaattctg attttgaagt cacatagact ggcgtttcta 
     3601 aaacaaaata tgcttgataa cctttatcag atttgataat catagtaggc ataaaaccta 
     3661 aatcaatagc ggttgttaaa atatcgcttg ctgaaatagt ttcttttgcc gtgtgaatat 
     3721 caaaatcaat aaagaaggta ttgatttgtc ttaaattgtt ttcagaatgt cctttcgtgt 
     3781 atgaacggtt ttcgtctgca tacgttccat aacgataaac gttgggtgtc caatgtgtaa 
     3841 atgtatcttg attttcttga atcgcttcct cggaagtcag aacaacacca cgaccgccaa 
     3901 tcatgcttga ttttgagcga tacgcaaaaa tagccccttt gcttttacct ggcttggtag 
     3961 tgattgagcg aattttacta tttttaaatt tgtactttaa caagccgtca tgaagcacag 
     4021 tttctacaac aaaagggata ttcattcagc tgttctcctt tcctataaat cctataaaat 
     4081 aggttgttta attaacttgg tttgcttttt cattcaactg tttcaatatt gcatgttttg 
     4141 aaaaagattt ttttccttta taagtcaatt tttttccact aatcgaataa attattttgt 
     4201 tattttctat taacttatat atataatctt ccccctccga agaaaaatac ttatctgatt 
     4261 ttgtttctaa gtagatattt ctcttttcta actctttctt aaacgtttct agtgtataga 
     4321 tatttgctaa ttttcttatc tccaataaac tattttttat ataagtttta cattcatcat 
     4381 gattcataca aactccacct tctataaatg aatacaaaaa aagcaatcaa acgatttccg 
     4441 attgattgct taacaattct taaattcagt agcttagata cttgaaaact ctctgatttc 
     4501 cctatataat gatagtacgg ttatataccg tcttcaaaca aagttaatta aataacttct 
     4561 tacgagggaa gagttcatct gactaactga taagcgttgg tttggcaatc ttatcgggct 
     4621 atgcatttat aaaatgtcgt caaacatttt ataaatgtgt catggctctt ttttcgtttc 
     4681 tattcagttc gttgtttcgt tatatctagt ataccgcttt taaaaaaaaa taagcaacga 
     4741 tttcgtgcat tattcacacg aagtcattgc ttttttcttc ttccatttct aaatccaatg 
     4801 ttacttgttc tgattctgtt tctggctctg gttctgttgg ctcatttggg attaaatcca 
     4861 ctactagcgt tgagttagtt aactttgcaa tttgttctag tgtttttatg gttggatctg 
     4921 attttcctga ttctattcgt gaataatttg atctactcat ttctaattct tggggtaccg 
     4981 ccagcatttc ggaaaaaaac cacgctaagg attttttcta taaaaagagc cgttatatta 
     5041 agaataaaac ggctctttta tacgtaaagg acgtaaattc atttgcccag tgtcatgtaa 
     5101 tccttcaaat ttgtattctc caagaaaatt gatatgttcc catcctaacg gccacgcata 
     5161 tggcattaaa tcttctctaa attctcctct tgcttttaat tcttctacgg ctttttccat 
     5221 atatacagtg ttccacacac ttatagcgtt aataattatg tttagtgcac tagctctttg 
     5281 taactggtct tggagagcac gttctctaaa ttctccacgt tgtccaaaaa atatagttct 
     5341 agctaatgca ttgattgctt ctcctttatt taaacctttt tgaacccgtc tccttacggc 
     5401 tttattagat atgtaatcca gcgtaaagag ggttttctcg attcgtccca tttctccaag 
     5461 tgctgttgcg agtttatttt gtcttgcata tgatccgagc ttccccatga taagagcgct 
     5521 agggacctct ttagctcctt ggaagctgtc agtagtatac ctaataattt atctacattc 
     5581 cctttagtaa cgtgtaactt tccaaattta caaaagcgac tcatagaatt atttcctccc 
     5641 gttaaataat agataactat taaaaataga caatacttgc tcataagtaa cggtacttaa 
     5701 attgtttact ttggcgtgtt tcattgcttg atgaaactga tttttagtaa acagttgacg 
     5761 atattctcga ttgacccatt ttgaaacaaa gtacgtatat agcttccaat atttatctgg 
     5821 aacatctgtg gtatggcggg taagttttat taagacactg tttacttttg gtttaggatg 
     5881 aaagcattcc gctggcagct taagcaattg ctgaatcgag acttgagtgt gcaagagcaa 
     5941 ccctagtgtt cggtgaatat ccaaggtacg cttgtagaat ccttcttcaa caatcagata 
     6001 gatgtcagac gcatggcttt caaaaaccac ttttttaata atttgtgtgc ttaaatggta 
     6061 aggaatactc ccaacaattt tatacctctg tttgttaggg aattgaaact gtagaatatc 
     6121 ttggtgaatt aaagtgacac gagtattcag ttttaatttt tctgacgata agttgaatag 
     6181 atgactgtct aattcaatag acgttacctg tttacttatt ttagccagtt tcgtcgttaa 
     6241 atgcccttta cctgttccaa tttcgtaaac ggtatcggtt tcttttaaat tcaattgttt 
     6301 tattatttgg ttgagtactt tttcactcgt taaaaagttt tgagaatatt ttatattttt 
     6361 gttcatgtaa tcactccttc ttaattacaa atttttagca tctaatttaa cttcaattcc 
     6421 tattatacaa aattttaaga tactgcacta tcaacacact cttaagtttg cttctaagtc 
     6481 ttatttccat aacttctttt acgtttccgc cattctttgc tgtttcgatt tttatgatat 
     6541 ggtgcaagtc agcacgaaca cgaaccgtct tatctcccat tatatctttt tttgcactga 
     6601 ttggtgtatc atttcgtttt tcttttgcgc ttcttgataa aagggatagt aattcattcc 
     6661 tggttgcaaa ttttgaaaac cgctacggat cacatctttt tctaaactat tgatccatag 
     6721 tcttttatac gttttatctt tagaaaaggc atttgcttta tgaatgatcg accaggcaat 
     6781 gttttcgcct tctctgtcgc tatctgttgc gacaatgatt gtatttgctt gttttaaaag 
     6841 ttcagcaaca attttaaact gctttttttt atctgttgcc acttcaaaat cgtatcgatt 
     6901 ctaggcttca tgtggcagga gaaaaaaggc tgcaccggtg cgtcagcaga atatgtgata 
     6961 caggatatat tccgcttcct cgctcactga ctcgctacgc tcggtcgttc gactgcggcg 
     7021 agcggaaatg gcttacgaac ggggcggaga tttcctggaa gatgccagga agatacttaa 
     7081 cagggaagtg agagggccgc ggaagctagc ttcgaggaac tttcggcggc tttgctgtgc 
     7141 gacaggctca cgtctaaaag gaaataaatc atgggtcata aaattatcac gttgtccggc 
     7201 gcggcgacgg atgttctgta tgcgctgttt ttccgtggcg cgttgctgtc tggtgatctg 
     7261 ccttctaaat ctggcacagc cgaattgcgc gagcttggtt ttgctgaaac cagacacaca 
     7321 gcaactgaat accagaaaga aaatcacttt acctttctga catcagaagg gcagaaattt 
     7381 gccgttgaac acctggtcaa tacgcgtttt ggtgagcagc aatattgcgc ttcgatgacg 
     7441 cttggcgttg agattgatac ctctgctgca caaaaggcaa tcgacgagct ggaccagcgc 
     7501 attcgtgaca ccgtctcctt cgaacttatt cgcaatggag tgtcattcat caaggacgcc 
     7561 gctatcgcaa atggtgctat ccacgcagcg gcaatcgaaa cacctcagcc ggtgaccaat 
     7621 atctacaaca tcagccttgg tatccagcgt gatgagccag cgcagaacaa ggtaaccgtc 
     7681 agtgccgata agttcaaagt taaacctggt gttgatacca acattgaaac gttgatcgaa 
     7741 aacgcgctga aaaacgctgc tgaatgtgcg gcgctggatg tcacaaagca aatggcagca 
     7801 gacaagaaag cgatggatga actggcttcc tatgtccgca cggccatcat gatggaatgt 
     7861 ttccccggtg gtgttatctg gcagcagtgc cgtcgatagt atgcaattga taattattat 
     7921 catttgcggg tcctttccgg cgatccgcct tgttacgggg cggcgacctc gcgggttttc 
     7981 gctatttatg aaaattttcc ggtttaaggc gtttccgttc ttcttcgtca taacttaatg 
     8041 tttttattta aaataccctc tgaaaagaaa ggaaacgaca ggtgctgaaa gcgagctttt 
     8101 tggcctctgt cgtttccttt ctctgttttt gtccgtggaa tgaacaatgg aagtcaacaa 
     8161 aaagcagagc ttatcgatga tccgcggcaa agcc 
//
