LOCUS       pBT16                   7669 bp    DNA     circular      5-AUG-2015
DEFINITION  LMBP 9600.
  
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|741438709|
COMMENT     VNTNAME|pBT16|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Phagemid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTEXTCHREPL|Yeast
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pBT16" 0 7669 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 1 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "AgeI" "ApaI" "BamHI" "BglI" "BglII" "BstXI" "DpnI" "DraI" 
COMMENT         "Eco47III" "EcoRI" "EcoRV" "HaeII" "HincII" "HindIII" "HpaI" "KpnI" 
COMMENT         "NarI" "NcoI" "NdeI" "NheI" "NotI" "NsiI" "PstI" "PvuI" "PvuII" "RsaI" 
COMMENT         "SacI" "SacII" "SalI" "ScaI" "SpeI" "SspI" "StuI" "XbaI" "XhoI" "XmaI" 
COMMENT         "XmnI") (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") 
COMMENT        (CObList) 1 "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 
COMMENT        0 (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 7669 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "AgeI" "accggt" 2 0 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "ApaI" "gggccc" 6 0 1 790 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 2 1816 0 6289 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "BglII" "agatct" 2 0 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "BstXI" "ccannnnnntgg" 9 0 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "DpnI" "gatc" 3 0 26 1092 0 1843 0 2175 0 2717 
COMMENT            0 2744 0 3422 0 3483 0 3510 0 3777 0 3912 0 4758 0 5844 0 5919 0 
COMMENT            5930 0 5938 0 6016 0 6028 0 6133 0 6474 0 6492 0 6538 0 6796 0 6813 
COMMENT            0 6849 0 7229 0 7599 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "DraI" "tttaaa" 4 0 8 2026 0 2063 0 3716 0 6035 
COMMENT            0 6054 0 6746 0 7126 0 7435 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "Eco47III" "agcgct" 4 0 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 2558 0 3691 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 2 600 0 3752 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "HaeII" "rgcgcy" 6 0 5 1719 0 1727 0 2642 0 
COMMENT             5154 0 5524 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "HincII" "gtyrac" 4 0 2 918 0 1054 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "HindIII" "aagctt" 2 0 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "HpaI" "gttaac" 4 0 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 4882 0 0 "") 
COMMENT        #16=(CRSite (CStringList) "NarI" "ggcgcc" 3 0 0 0 "") 
COMMENT        #17=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 2 617 0 2584 0 0 "") 
COMMENT        #18=(CRSite (CStringList) "NdeI" "catatg" 3 0 1 325 0 0 "") 
COMMENT        #19=(CRSite (CStringList) "NheI" "gctagc" 2 0 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "NsiI" "atgcat" 6 0 2 1239 0 4247 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 394 0 3545 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 3 1844 0 3778 0 6539 0 0 
COMMENT             "") 
COMMENT        #24=(CRSite (CStringList) "PvuII" "cagctg" 4 0 4 1873 0 2601 0 3927 0 
COMMENT             5100 0 0 "") 
COMMENT        #25=(CRSite (CStringList) "RsaI" "gtac" 3 0 13 170 0 528 0 661 0 724 0 
COMMENT             912 0 2320 0 3242 0 3781 0 4223 0 4491 0 4880 0 6649 0 7413 0 0 "") 
COMMENT        #26=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 1998 0 0 "") 
COMMENT        #27=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 0 0 "") 
COMMENT        #28=(CRSite (CStringList) "SalI" "gtcgac" 2 0 0 0 "") 
COMMENT        #29=(CRSite (CStringList) "ScaI" "agtact" 4 0 2 724 0 6649 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "SpeI" "actagt" 2 0 3 2397 0 2576 0 2778 0 0 
COMMENT             "") 
COMMENT        #31=(CRSite (CStringList) "SspI" "aatatt" 4 0 6 1360 0 3040 0 3478 0 
COMMENT             4040 0 4156 0 6973 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "StuI" "aggcct" 4 0 1 848 0 0 "") 
COMMENT        #33=(CRSite (CStringList) "XbaI" "tctaga" 2 0 0 0 "") 
COMMENT        #34=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 0 0 "") 
COMMENT        #35=(CRSite (CStringList) "XmaI" "cccggg" 2 0 0 0 "") 
COMMENT        #36=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 5 2125 0 2507 0 2819 0 
COMMENT             6768 0 7289 0 0 "")) 
COMMENT       (CObList 
COMMENT        #37=(CFSignal (CObList) "PMB1" 33 0 0 5337 5337 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #38=(CFSignal (CObList) "URA3" 4 0 0 411 1214 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #39=(CFSignal (CObList) "CEN6" 21 0 0 7097 7213 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #40=(CFSignal (CObList) "F1_ORI" 33 0 0 1567 1666 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #41=(CFSignal (CObList) "T3_P" 30 0 1 4894 4913 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #42=(CFSignal (CObList) "T7_P" 30 0 0 1966 1985 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #43=(CFSignal (CObList) "AP(R)" 4 0 1 6095 6955 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #44=(CFSignal (CObList) "ARSH4" 21 0 0 7214 7588 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #45=(CFSignal (CObList) "S. cerevisiae YPK1" 4 0 1 2422 4461 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #46=(CFSignal (CObList) "HA epitope" 22 0 1 4465 4494 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #47=(CFSignal (CObList) "S. cerevisiae YPK1 promoter" 29 0 1 4498 4873 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #48=(CFSignal (CObList) "S. cerevisiae YPK1 terminator" 43 0 1 2002 2421 
COMMENT             0 (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #49=(CFSignal (CObList) "L424G" 62 0 0 3193 3195 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #50=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #51=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "pBT16" 2) 5 "" 0 
COMMENT                   4) 
COMMENT              #52=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #53=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Phagemid 'pBT16'" 1) 
COMMENT                     #54=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "ATCC 77145: Yeast centromere vector pRS316 with URA3 marker, complete sequence." 
COMMENT                          1) 
COMMENT                     #55=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: NCBI Entrez" 
COMMENT                          1) 
COMMENT                     #56=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 08/05/15 11:11" 1) 
COMMENT                     #57=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 08/05/15 11:11" 1) 
COMMENT                     #58=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 7669 bp" 1) 
COMMENT                     #59=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #60=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #61=(CFolderPar 
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COMMENT                    (CObjectList 
COMMENT                     #62=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Keywords: ATCC" 1))) "Standard Fields") 
COMMENT              #63=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 5 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #64=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "NCBI Entrez" 1) 
COMMENT                     #65=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "National Center for Biotechnology Information" 1) 
COMMENT                     #66=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "National Library of Medicine" 1) 
COMMENT                     #67=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Building 38A, Room 8N805" 1) 
COMMENT                     #68=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Bethesda, MD 20894, U.S.A." 1) 
COMMENT                     #69=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "TEL: (301)496-2475  FAX: (301)480-9241" 1) 
COMMENT                     #70=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "EMAIL: <a href=\"mailto:info@ncbi.nlm.nih.gov\">info@ncbi.nlm.nih.gov</a>" 
COMMENT                          1))) "Original Author") 
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COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #72=(CFolderPar 
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COMMENT                   "Annotations") 
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COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
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COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 411 (3 #38# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 411   End: 1214" 1) 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "orotidine-5'-phosphate" 1))) "URA3") 
COMMENT                            #78=(CFolderPar 
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COMMENT                                  (CParagraph 2422 (3 #45# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #79=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2422  End: 4461 (Complementary)" 
COMMENT                                        1))) "S. cerevisiae YPK1") 
COMMENT                            #80=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 6095 (3 #43# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 6095  End: 6955 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "bla beta-lactamase, ampR ampicillin resistance" 
COMMENT                                        1))) "AP(R)"))) "CDS (3 total)") 
COMMENT                     #83=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #84=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 7097 (3 #39# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #85=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 7097  End: 7213" 1) 
COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "centromere VI" 1))) "CEN6") 
COMMENT                            #87=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 7214 (3 #44# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #88=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 7214  End: 7588" 1) 
COMMENT                                   #89=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "autonomous replicating sequence histone 4" 
COMMENT                                        1))) "ARSH4"))) 
COMMENT                          "Misc. Feature (2 total)") 
COMMENT                     #90=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 22 (7 22 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #91=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4465 (3 #46# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #92=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4465  End: 4494 (Complementary)" 
COMMENT                                        1))) "HA epitope"))) 
COMMENT                          "Misc. Marker (1 total)") 
COMMENT                     #93=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #94=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4498 (3 #47# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #95=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4498  End: 4873 (Complementary)" 
COMMENT                                        1))) "S. cerevisiae YPK1 promoter"))) 
COMMENT                          "Promoter Eukaryotic (1 total)") 
COMMENT                     #96=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #97=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1966 (3 #42# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #98=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1966  End: 1985" 1) 
COMMENT                                   #99=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "T7 promoter" 1))) "T7_P") 
COMMENT                            #100=(CFolderPar 
COMMENT                                  (CGroupPar 
COMMENT                                   (CParagraph 4894 (3 #41# 0) 1 2 2 0 194) 
COMMENT                                   (CObjectList 
COMMENT                                    #101=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Start: 4894  End: 4913 (Complementary)" 
COMMENT                                          1) 
COMMENT                                    #102=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "T3 promoter" 1))) "T3_P"))) 
COMMENT                          "Promoter Prokaryotic (2 total)") 
COMMENT                     #103=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
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COMMENT                                   (CGroupPar 
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COMMENT                                    (CObjectList 
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COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 1567  End: 1666" 1) 
COMMENT                                     #106=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "origin of replication" 1))) "F1_ORI") 
COMMENT                             #107=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 5337 (3 #37# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #108=(CLinePar 
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COMMENT                                           "Start: 5337  End: 5337" 1) 
COMMENT                                     #109=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "origin of replication" 1))) "PMB1"))) 
COMMENT                           "Replication Origin (2 total)") 
COMMENT                     #110=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #111=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 2002 (3 #48# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #112=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 2002  End: 2421 (Complementary)" 
COMMENT                                           1))) "S. cerevisiae YPK1 terminator"))) 
COMMENT                           "Terminator (1 total)") 
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COMMENT                            (CObjectList 
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COMMENT                                           "Start: 3193  End: 3195" 1) 
COMMENT                                     #116=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "WT: TTA" 1))) "L424G"))) 
COMMENT                           "Mutation (1 total)"))) "Feature Map") 
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COMMENT                             (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "AgeI: 0 sites") 1 5 "ACCGGT" 
COMMENT                            "TGGCCA") 
COMMENT                      #119=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "BamHI: 0 sites") 1 5 "GGATCC" 
COMMENT                            "CCTAGG") 
COMMENT                      #120=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "BglII: 0 sites") 1 5 "AGATCT" 
COMMENT                            "TCTAGA") 
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COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "BstXI: 0 sites") 8 4 
COMMENT                            "CCANNNNNNTGG" "GGTNNNNNNACC") 
COMMENT                      #122=(CRSFolderPar 
COMMENT                            (CFolderPar 
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COMMENT                              (CObjectList)) "Eco47III: 0 sites") 3 3 "AGCGCT" 
COMMENT                            "TCGCGA") 
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COMMENT                              (CObjectList)) "HindIII: 0 sites") 1 5 "AAGCTT" 
COMMENT                            "TTCGAA") 
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COMMENT                            (CFolderPar 
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COMMENT                              (CObjectList)) "HpaI: 0 sites") 3 3 "GTTAAC" 
COMMENT                            "CAATTG") 
COMMENT                      #125=(CRSFolderPar 
COMMENT                            (CFolderPar 
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COMMENT                              (CObjectList)) "NarI: 0 sites") 2 4 "GGCGCC" 
COMMENT                            "CCGCGG") 
COMMENT                      #126=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "NheI: 0 sites") 1 5 "GCTAGC" 
COMMENT                            "CGATCG") 
COMMENT                      #127=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "NotI: 0 sites") 2 6 "GCGGCCGC" 
COMMENT                            "CGCCGGCG") 
COMMENT                      #128=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "SacII: 0 sites") 4 2 "CCGCGG" 
COMMENT                            "GGCGCC") 
COMMENT                      #129=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "SalI: 0 sites") 1 5 "GTCGAC" 
COMMENT                            "CAGCTG") 
COMMENT                      #130=(CRSFolderPar 
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COMMENT                             (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "XbaI: 0 sites") 1 5 "TCTAGA" 
COMMENT                            "AGATCT") 
COMMENT                      #131=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "XhoI: 0 sites") 1 5 "CTCGAG" 
COMMENT                            "GAGCTC") 
COMMENT                      #132=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "XmaI: 0 sites") 1 5 "CCCGGG" 
COMMENT                            "GGGCCC") 
COMMENT                      #133=(CRSFolderPar 
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COMMENT                                            (CParagraph 0 (1 #1# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 790 " 1))))) "ApaI: 1 site") 5 
COMMENT                            1 "GGGCCC" "CCCGGG") 
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FEATURES             Location/Qualifiers
     rep_origin      5337..5337
                     /vntifkey="33"
                     /label=PMB1
                     /note="origin of replication"
     CDS             411..1214
                     /vntifkey="4"
                     /label=URA3
                     /note="orotidine-5'-phosphate"
     misc_feature    7097..7213
                     /vntifkey="21"
                     /label=CEN6
                     /note="centromere VI"
     rep_origin      1567..1666
                     /vntifkey="33"
                     /label=F1_ORI
                     /note="origin of replication"
     promoter        complement(4894..4913)
                     /vntifkey="30"
                     /label=T3_P
                     /note="T3 promoter"
     promoter        1966..1985
                     /vntifkey="30"
                     /label=T7_P
                     /note="T7 promoter"
     CDS             complement(6095..6955)
                     /vntifkey="4"
                     /label=AP(R)
                     /note="bla beta-lactamase, ampR ampicillin resistance"
     misc_feature    7214..7588
                     /vntifkey="21"
                     /label=ARSH4
                     /note="autonomous replicating sequence histone 4"
     CDS             complement(2422..4461)
                     /vntifkey="4"
                     /label=S.\cerevisiae\YPK1
     misc_marker     complement(4465..4494)
                     /vntifkey="22"
                     /label=HA\epitope
     promoter        complement(4498..4873)
                     /vntifkey="29"
                     /label=S.\cerevisiae\YPK1\promoter
     terminator      complement(2002..2421)
                     /vntifkey="43"
                     /label=S.\cerevisiae\YPK1\terminator
     mutation        3193..3195
                     /vntifkey="62"
                     /label=L424G
                     /note="WT: TTA"
BASE COUNT     2237 a      1595 c      1654 g      2171 t        12 others
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accacgcttt tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc 
      241 tttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca 
      301 gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat 
      361 tcttaaccca actgcacaga acaaaaacct gcaggaaacg aagataaatc atgtcgaaag 
      421 ctacatataa ggaacgtgct gctactcatc ctagtcctgt tgctgccaag ctatttaata 
      481 tcatgcacga aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc accaaggaat 
      541 tactggagtt agttgaagca ttaggtccca aaatttgttt actaaaaaca catgtggata 
      601 tcttgactga tttttccatg gagggcacag ttaagccgct aaaggcatta tccgccaagt 
      661 acaatttttt actcttcgaa gacagaaaat ttgctgacat tggtaataca gtcaaattgc 
      721 agtactctgc gggtgtatac agaatagcag aatgggcaga cattacgaat gcacacggtg 
      781 tggtgggccc aggtattgtt agcggtttga agcaggcggc agaagaagta acaaaggaac 
      841 ctagaggcct tttgatgtta gcagaattgt catgcaaggg ctccctatct actggagaat 
      901 atactaaggg tactgttgac attgcgaaga gcgacaaaga ttttgttatc ggctttattg 
      961 ctcaaagaga catgggtgga agagatgaag gttacgattg gttgattatg acacccggtg 
     1021 tgggtttaga tgacaaggga gacgcattgg gtcaacagta tagaaccgtg gatgatgtgg 
     1081 tctctacagg atctgacatt attattgttg gaagaggact atttgcaaag ggaagggatg 
     1141 ctaaggtaga gggtgaacgt tacagaaaag caggctggga agcatatttg agaagatgcg 
     1201 gccagcaaaa ctaaaaaact gtattataag taaatgcatg tatactaaac tcacaaatta 
     1261 gagcttcaat ttaattatat cagttattac cctgcggtgt gaaataccgc acagatgcgt 
     1321 aaggagaaaa taccgcatca ggaaattgta aacgttaata ttttgttaaa attcgcgtta 
     1381 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat 
     1441 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca 
     1501 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc 
     1561 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta 
     1621 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg 
     1681 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg 
     1741 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg 
     1801 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg 
     1861 ctattacgcc agctggcgaa ggggggatgt gctgcaaggc gattaagttg ggtaacgcca 
     1921 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta 
     1981 tagggcgaat tggagctcnn naacaatggg gtcttgggtt aatttaaagt cgaacgctga 
     2041 ttaaaacaac ctttatattt ttaaaaggag aagctactgc aaacgagaga tgctcatcaa 
     2101 aatatctttt atacaaggag aaaattttca acaccttttt ttttttttat tttctgccgc 
     2161 cccatcgaca cggatctcgg aagggtaaga gaggaaatcc tttatctaat cagaataagc 
     2221 tgaggcaata cgctatgacc atgagacaca agccacacat gtgtgaagta ttgcccacgt 
     2281 tcgaaatata aatcctagaa cttaaattgc gccattggta cagttgcttc atcttgaaca 
     2341 cagtaagtaa cggaaaagaa aacctttctt tttatataaa gtatgtcatg agtaaactag 
     2401 ttgataatgt attcactaag tctatctaat gcttctacct tgcaccattg agctacctag 
     2461 ctgttcattt ccaacgtatg tccagccacc aaattgcttt tgaacacttt cactcaagta 
     2521 ttcatctacc acactatcaa ttggcttctc cctagtgaat tcctcatcga aattactagt 
     2581 atccatggaa ttactaacag ctggtttata tggtggaata taacccttca tcaacaggcg 
     2641 cttccatgat aattggctga aaaaaggatg gttccgaatt tcgtcggcac cattgtagcc 
     2701 caatcttctt gtcggatcac ggctcaataa tccaatcaat agatcctttg catctctatc 
     2761 aaatccatct gggaaaacta gtggctcctg tagaatcttc ttatacattt ttggaacatc 
     2821 ttcatcataa taaggaggaa gacctgtgag catctcgtat aacaagactc ccaatgtcca 
     2881 ccaatctact gcctttgtat agcctaaacc tagcaatagt tctggtgcca agtattctgg 
     2941 ggtcccacaa aaagtatctg tcttatcatc atccttcata ttcaatttgc atagcccgaa 
     3001 atcacaaagg gcaatgtggc cttgataatc caataaaata ttctctggtt tcaaatcacg 
     3061 atagacaaca tctagtttat gcaagttgtc tagcgcacat aacaattctg cggtataaaa 
     3121 tctggcacgt gataaatcaa accttccttc cttttgtaga tgataaaaca actcaccacc 
     3181 attgataaac gctccaacaa agtataattt ttccggtgat tggaaagaaa acttcaaagg 
     3241 tacaataaat ggacaatcaa cacgtgctag aacggttctt tctgccaaag tatgcgtgac 
     3301 ttcggattta gagacaatgt atgattttct gattgccttc aaagcgtata ctttttgtgt 
     3361 atctttcttt ctgacttgca tcactttacc aaacgaaccc ttaccgataa ctttcaaaag 
     3421 atcgaaatca tcgatggaca aaggtttatt tctggaaggt ttataatcaa tactaatatt 
     3481 gatcttaccc aagccattat ccaatgtgat ccagtggtga ttatatagtc taatactagc 
     3541 tgcagagtca aaacttaagt tgatgggcaa atggaaagaa tctaaatgta tatcttgatt 
     3601 ggaatttatt ttatcaaaaa tggtctgtag cttctcgtct tgcaaaccca tctcctgttg 
     3661 ccatgtcttc gagggcaaaa gaatggacgg aattcttgca aatacatcaa tctttaaata 
     3721 aggtaatttc cttgtcacat caaaggtaga tatcttattg aaaccaggtt tagcgatcgt 
     3781 accatactct gcttcaatag ttgcaactgt attatcaaat tcaatggtga aatatagtaa 
     3841 gggatttaag gtcgaagacc ctggtaacat gatagtagat gggatgaact ttgctgctga 
     3901 ctcgctagag atcaaatctt catcagctgg accttggttc tttatttgaa cacgggacag 
     3961 ttgtgcaatt aaagcatcta cttctttact aatctcctta tgtggcggtg gaacacccga 
     4021 ggccaatagt ttattcaata tttgctcgct tgaggtaatc ggaaaaggta aaataaagtc 
     4081 atcaccatta tatactttga tagtcatcat tcctgaactt gaggtggcat cattgggtgt 
     4141 ccctgtagaa ccaatatttg ctgtttcatc taaattttga gatgagttcg tgttttctga 
     4201 attaccacca tttgaatctc gtacagtaga ggtcgatgag gatgcatcgt atgaaacacg 
     4261 aaccgggacc actgatgaat ttgatggatt tatcgtgccc ttcctgtcta aagatgaacg 
     4321 agtaattgaa gcatcatgtt cccccgcagt tgcttgatta ttctgctgtt cttccttttt 
     4381 agaggagtga aaaaacccac tatgtttggc ttctttttct tctttagatt ttccaaactt 
     4441 aaactttgac ttccaagaat annnagcgta atctggaaca tcgtatgggt acatnnnttt 
     4501 caggaactgt attaatgttt gttgatatga gaggaacttt gaagtgtcaa tttcccgctg 
     4561 tatacaaaga aattgtagag taatctatat caaagaatat aaaggctttg aaatttaacc 
     4621 aaacacaaac aaaccaattc taaaaagtat gggagcgaaa aataactcaa ctgttgttgg 
     4681 tatgacgata gtgttgatta ccgttggctc gttcgggata agcatttttt aagccttcca 
     4741 ataggggcat gttttgatct tgttttcctg tagcgcggct ctgccgcgaa gaaagtcgcg 
     4801 agtaacgaaa aacaaaaaca atgttgtatt atgaagctaa ttgataagtc aatcagcaaa 
     4861 aaatagatat actnnnggta cccagctttt gttcccttta gtgagggtta attccgagct 
     4921 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 
     4981 acaacatagg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgaggtaac 
     5041 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 
     5101 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 
     5161 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 
     5221 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 
     5281 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 
     5341 ataggctcgg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 
     5401 acccgacagg actataaaga taccaggcgt tcccccctgg aagctccctc gtgcgctctc 
     5461 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 
     5521 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 
     5581 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 
     5641 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 
     5701 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 
     5761 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 
     5821 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 
     5881 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 
     5941 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 
     6001 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 
     6061 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 
     6121 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactgccc gtcgtgtaga 
     6181 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 
     6241 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 
     6301 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 
     6361 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 
     6421 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 
     6481 gagttacatg atcccccatg ttgtgaaaaa aagcggttag ctccttcggt cctccgatcg 
     6541 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 
     6601 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 
     6661 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 
     6721 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 
     6781 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 
     6841 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 
     6901 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 
     6961 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 
     7021 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 
     7081 cacctgggtc cttttcatca cgtgctataa aaataattat aatttaaatt ttttaatata 
     7141 aatatataaa ttaaaaatag aaagtaaaaa aagaaattaa agaaaaaata gtttttgttt 
     7201 tccgaagatg taaaagactc tagggggatc gccaacaaat actacctttt atcttgctct 
     7261 tcctgctctc aggtattaat gccgaattgt ttcatcttgt ctgtgtagaa gaccacacac 
     7321 gaaaatcctg tgattttaca ttttacttat cgttaatcga atgtatatct atttaatctg 
     7381 cttttcttgt ctaataaata tatatgtaaa gtacgctttt tgttgaaatt ttttaaacct 
     7441 ttgtttattt ttttttcttc attccgtaac tcttctacct tctttattta ctttctaaaa 
     7501 tccaaataca aaacataaaa ataaataaac acagagtaaa ttcccaaatt attccatcat 
     7561 taaaagatac gaggcgcgtg taagttacag gcaagcgatc cgtcctaaga aaccattatt 
     7621 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtc 
//
