LOCUS       pCAGGS-E-MyD88-L265P        5666 bp    DNA     circular     17-MAY-2011
DEFINITION  LMBP 9635.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 9635
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 11-MAR-2009
            
            
            
            
COMMENT     VNTDATE|590432875|
COMMENT     VNTNAME|pCAGGS-E-MyD88-L265P|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-E-MyD88-L265P" 0 5666 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 3 "MAIN" 1 1 1 1 0 0 1 1 1 0 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) (CStringList "BamHI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 3 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 5666 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 3 89 0 1606 0 1943 0 0 "")) 
COMMENT       (CObList 
COMMENT        #1=(CFSignal (CObList) "3' UTR of \337-globin, incl. polyA" 43 0 0 956 
COMMENT            1047 0 (CStringList) (CStringList) 1 1 1 1 "956..1047") 
COMMENT        #2=(CFSignal (CObList) "3' flanking region of rabbit \337-globin" 21 0 0 
COMMENT            1048 1404 0 (CStringList) (CStringList) 1 1 1 1 "1048..1404") 
COMMENT        #3=(CFSignal (CObList) "lac promoter" 30 0 1 1482 1564 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1482..1564)") 
COMMENT        #4=(CFSignal (CObList) "SV40 ori" 33 0 0 1706 1781 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1706..1781") 
COMMENT        #5=(CFSignal (CObList) "SV40 polyA early" 43 0 0 1812 1886 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "1812..1886") 
COMMENT        #6=(CFSignal (CObList) "SV40 polyA late" 43 0 1 1812 1886 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(1812..1886)") 
COMMENT        #7=(CFSignal (CObList) "pMB1 ori" 33 0 1 2001 2402 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2001..2402)") 
COMMENT        #8=(CFSignal (CObList) "ampicillin resistance" 4 0 1 2943 3803 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(2943..3803)") 
COMMENT        #9=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 3939 4316 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "3939..4316") 
COMMENT        #10=(CFSignal (CObList) "AG promoter" 29 0 0 4322 5663 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4322..5663") 
COMMENT        #11=(CFSignal (CObList) "intron AG promoter" 15 0 0 4691 5615 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4691..5615") 
COMMENT        #12=(CFSignal (CObList) "mMyD88" 4 0 0 64 954 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "64..954") 
COMMENT        #13=(CFSignal (CObList) "E-tag" 22 0 0 16 54 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "16..54") 
COMMENT        #14=(CFSignal (CObList) "L265P" 21 0 0 817 819 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
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COMMENT                          "DNA Plasmid 'pCAGGS-E-MyD88-L265P'" 1) 
COMMENT                     #19=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 9635." 
COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 05/17/11 05:07" 1) 
COMMENT                     #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 05/17/11 05:07" 1) 
COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5666 bp" 1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 3-MAR-2010" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                                        "   complement(2943..3803)" 1))) 
COMMENT                                 "ampicillin resistance"))) "CDS (2 total)") 
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COMMENT                                        "chicken \337-actin/rabbit \337-globin hybrid intron" 
COMMENT                                        1) 
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COMMENT                                        "chicken \337-actin/rabbit \337-globin hybrid promoter" 
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COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT                                        "E. coli lac operon promoter" 1) 
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COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
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COMMENT                                 "pMB1 ori"))) "Replication Origin (2 total)") 
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FEATURES             Location/Qualifiers
     terminator      956..1047
                     /vntifkey="43"
                     /label=3'\UTR\of\ß-globin,\incl.\polyA
     misc_feature    1048..1404
                     /vntifkey="21"
                     /label=3'\flanking\region\of\rabbit\ß-globin
     promoter        complement(1482..1564)
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="E. coli lac operon promoter"
     rep_origin      1706..1781
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     terminator      1812..1886
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     terminator      complement(1812..1886)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     rep_origin      complement(2001..2402)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     CDS             complement(2943..3803)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        3939..4316
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
                     /note="human CMV-IE enhancer"
     promoter        4322..5663
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     intron          4691..5615
                     /vntifkey="15"
                     /label=intron\AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid intron"
     CDS             64..954
                     /vntifkey="4"
                     /label=mMyD88
     misc_marker     16..54
                     /vntifkey="22"
                     /label=E-tag
     misc_feature    817..819
                     /vntifkey="21"
                     /label=L265P
BASE COUNT     1187 a      1514 c      1587 g      1378 t 
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 
       61 gcgatgtctg cgggagaccc ccgcgtggga tccgggtccc tggactcctt catgttctcc 
      121 atacccttgg tcgcgcttaa cgtgggagtg aggcgccgcc tatcgctgtt cttgaaccct 
      181 cggacgcccg tggcggccga ctggaccttg ctcccggagg agatgggctt cgagtacttg 
      241 gagatccgag agctggaaac gcgccctgac cccactcgca gtttgttgga tgcctggcag 
      301 gggcgctctg gcgcgtctgt cggcaggctg ctagagctgc tggccttgtt agaccgtgag 
      361 gatatactga aggagctgaa gtcgcgcatc gaggaggact gccagaaata cttaggtaag 
      421 cagcagaacc aggagtccga gaagccttta caggtggcca gagtggaaag cagtgtccca 
      481 caaacaaagg aactgggagg catcaccacc cttgatgacc ccctaggaca aacgccggaa 
      541 cttttcgatg cctttatctg ctactgcccc aacgatatcg agtttgtgca ggagatgatc 
      601 cggcaactag aacagacaga ctatcggctt aagttgtgtg tgtccgaccg tgacgtcctg 
      661 ccgggcacct gtgtctggtc cattgccagc gagctaattg agaaaaggtg tcgccgcatg 
      721 gtggtggttg tttctgacga ttatctacag agcaaggaat gtgacttcca gaccaagttt 
      781 gcactcagcc tgtctccagg tgtccaacag aagcgaccga ttcctattaa atacaaggcg 
      841 atgaagaagg actttcccag tatcctgcgg ttcatcacta tatgcgacta taccaaccct 
      901 tgcaccaagt cctggttctg gacccgcctt gccaaggctt tgtccctgcc ctgaagatct 
      961 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg 
     1021 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact 
     1081 cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag 
     1141 tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca 
     1201 tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg 
     1261 aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt 
     1321 ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc 
     1381 tgtccctctt ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt 
     1441 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 
     1501 gaagcataaa gtgtaaagcc tgggtgccta atgagtgagc taactcacat taattgcgtt 
     1561 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat 
     1621 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt 
     1681 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 
     1741 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt 
     1801 tgcaaaaagc taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 
     1861 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 
     1921 tcaatgtatc ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag 
     1981 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 
     2041 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 
     2101 atcagggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 
     2161 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 
     2221 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 
     2281 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 
     2341 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 
     2401 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 
     2461 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 
     2521 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 
     2581 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc 
     2641 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 
     2701 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 
     2761 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 
     2821 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 
     2881 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 
     2941 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 
     3001 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 
     3061 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 
     3121 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 
     3181 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 
     3241 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 
     3301 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 
     3361 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 
     3421 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 
     3481 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 
     3541 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 
     3601 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 
     3661 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 
     3721 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 
     3781 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 
     3841 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 
     3901 gttccgcgca catttccccg aaaagtgcca cctgggtcga cattgattat tgactagtta 
     3961 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac 
     4021 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc 
     4081 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt 
     4141 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac 
     4201 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac 
     4261 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt 
     4321 cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat 
     4381 tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggg 
     4441 gcccccccca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggaaaggtgc 
     4501 ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg 
     4561 gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgttgcgc gctgccttcc 
     4621 ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt 
     4681 actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt 
     4741 ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg 
     4801 ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc 
     4861 cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg 
     4921 tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg 
     4981 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg 
     5041 tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag ttgctgagca 
     5101 cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg 
     5161 cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg 
     5221 ctcgggggaa ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc 
     5281 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa 
     5341 tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga 
     5401 agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc 
     5461 gccgtcccct tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg 
     5521 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg 
     5581 ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg 
     5641 tgctgtctca tcattttggc aaagaa 
//

