LOCUS       pCAXL-Mx1FRB_A2G      7052 bp    DNA     circular UNA 21-FEB-2012
DEFINITION  LMBP 9647.
ACCESSION   pCAXL-Mx1_A2G
SOURCE      Saccharomyces cerevisiae (A/Victoria/3/1975(H3N2)
  ORGANISM  Saccharomyces cerevisiae
            Saccharomyces cerevisiae
            Eukaryota; Fungi; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
FEATURES             Location/Qualifiers
     promoter        join(6805..7052,1..1087)
                     /note="Oriinal location: 3484..4818"
                     /label=AG
     CDS             1109..3001
                     /note="NM_010846"
                     /label="A2G Mx1"
     CDS             3002..3022
                     /label=linker
     CDS             3023..3319
                     /label=FRB
     polyA_signal    3412..3503
                     /label="b-globin polyA"
     3'UTR           3504..3860
                     /label="3' FR of b-globin"
     promoter        3939..4022
                     /label="Lac promoter"
     rep_origin      complement(4466..4876)
                     /note="Oriinal location: complement(1145..1555)"
                     /label=ORI
     CDS             complement(5419..6276)
                     /note="Oriinal location: complement(2098..2955)"
                     /note="ORF: Frame #1   Start: atg (2098)   Stop:  taa
                     (2955)"
                     /label="Amp R"
     promoter        6416..6793
                     /label="hCMV-IE enhancer"
ORIGIN
        1 ATAAAAAGCG AAGCGCGCGG CGGGCGGGAG TCGCTGCGTT GCCTTCGCCC CGTGCCCCGC
       61 TCCGCGCCGC CTCGCGCCGC CCGCCCCGGC TCTGACTGAC CGCGTTACTC CCACAGGTGA
      121 GCGGGCGGGA CGGCCCTTCT CCTCCGGGCT GTAATTAGCG CTTGGTTTAA TGACGGCTCG
      181 TTTCTTTTCT GTGGCTGCGT GAAAGCCTTA AAGGGCTCCG GGAGGGCCCT TTGTGCGGGG
      241 GGGAGCGGCT CGGGGGGTGC GTGCGTGTGT GTGTGCGTGG GGAGCGCCGC GTGCGGCCCG
      301 CGCTGCCCGG CGGCTGTGAG CGCTGCGGGC GCGGCGCGGG GCTTTGTGCG CTCCGCGTGT
      361 GCGCGAGGGG AGCGCGGCCG GGGGCGGTGC CCCGCGGTGC GGGGGGGCTG CGAGGGGAAC
      421 AAAGGCTGCG TGCGGGGTGT GTGCGTGGGG GGGTGAGCAG GGGGTGTGGG CGCGGCGGTC
      481 GGGCTGTAAC CCCCCCCTGC ACCCCCCTCC CCGAGTTGCT GAGCACGGCC CGGCTTCGGG
      541 TGCGGGGCTC CGTGCGGGGC GTGGCGCGGG GCTCGCCGTG CCGGGCGGGG GGTGGCGGCA
      601 GGTGGGGGTG CCGGGCGGGG CGGGGCCGCC TCGGGCCGGG GAGGGCTCGG GGGAGGGGCG
      661 CGGCGGCCCC GGAGCGCCGG CGGCTGTCGA GGCGCGGCGA GCCGCAGCCA TTGCCTTTTA
      721 TGGTAATCGT GCGAGAGGGC GCAGGGACTT CCTTTGTCCC AAATCTGGCG GAGCCGAAAT
      781 CTGGGAGGCG CCGCCGCACC CCCTCTAGCG GGCGCGGGCG AAGCGGTGCG GCGCCGGCAG
      841 GAAGGAAATG GGCGGGGAGG GCCTTCGTGC GTCGCCGCGC CGCCGTCCCC TTCTCCATCT
      901 CCAGCCTCGG GGCTGCCGCA GGGGGACGGC TGCCTTCGGG GGGGACGGGG CAGGGCGGGG
      961 TTCGGCTTCT GGCGTGTGAC CGGCGGGNTC TAGANCCTCT GCTAACCATG TTCATGCCTT
     1021 CTTCTTTTTC CTACAGCTCC TGGGCAACGT GCTGGTTATT GTGCTGTCTC ATCATTTTGG
     1081 CAAAGAATTC CTCGAGAATT CGATTACGAT GGATTCTGTG AATAATCTGT GCAGGCACTA
     1141 TGAGGAGAAG GTGCGGCCCT GTATTGACCT CATCGACACC CTGAGGGCTC TGGGTGTGGA
     1201 GCAGGACCTG GCCCTGCCTG CCATCGCTGT CATTGGGGAC CAGAGTTCAG GGAAGAGCTC
     1261 TGTTCTGGAA GCACTGTCTG GAGTGGCCCT CCCCAGAGGC AGTGGTATTG TCACCAGATG
     1321 CCCTCTGGTG CTGAAATTGA GGAAGCTGAA AGAAGGAGAG GAGTGGAGAG GCAAAGTCTC
     1381 CTATGATGAC ATCGAAGTGG AGCTCTCTGA TCCTTCAGAG GTGGAAGAGG CCATTAACAA
     1441 GGGTCAGAAC TTCATTGCTG GGGTAGGCCT GGGGATCAGT GATAAGCTCA TTAGCCTGGA
     1501 TGTCAGCTCC CCAAATGTCC CAGACCTGAC TCTCATTGAC CTGCCTGGAA TTACCAGGGT
     1561 GGCTGTAGGC AACCAGCCTG CAGACATAGG ACGCCAGATC AAGAGACTTA TCAAGACATA
     1621 CATCCAAAAA CAAGAGACCA TCAACCTGGT GGTAGTCCCC AGCAATGTGG ACATTGCTAC
     1681 CACAGAGGCT CTGAGCATGG CTCAGGAGGT GGACCCTGAA GGGGATAGGA CCATAGGGGT
     1741 CTTGACCAAG CCTGATCTGG TGGACAGAGG TGCTGAAGGC AAGGTCTTGG ATGTGATGCG
     1801 GAACCTGGTG TATCCTCTGA AGAAGGGCTA CATGATAGTC AAGTGCAGAG GTCAGCAGGA
     1861 CATCCAAGAG CAGCTGAGCC TGACTGAGGC TTTTCAGAAA GAGCAAGTCT TCTTCAAGGA
     1921 TCACTCATAC TTCAGCATTC TTCTGGAAGA TGGGAAGGCC ACAGTGCCCT GCCTGGCAGA
     1981 GAGACTGACT GAGGAGCTCA CCTCCCACAT CTGTAAATCA CTGCCACTAT TGGAAGATCA
     2041 AATAAATAGC AGTCATCAGA GTGCAAGCGA GGAGCTGCAG AAGTACGGTG CAGACATACC
     2101 AGAAGATGAC AGAACGAGGA TGTCCTTTCT GGTGAATAAA ATCAGTGCCT TCAATAGGAA
     2161 TATCATGAAT TTGATACAAG CACAGGAAAC CGTATCAGAG GGAGACAGCC GGTTGTTTAC
     2221 CAAACTGCGA AATGAGTTTC TTGCTTGGGA TGATCATATT GAGGAATATT TTAAAAAAGA
     2281 TTCTCCTGAG GTACAAAGCA AGATGAAAGA ATTTGAAAAT CAGTATCGTG GCCGGGAGCT
     2341 GCCAGGGTTT GTGGACTACA AGGCATTTGA GAGCATCATC AAAAAGCGAG TCAAAGCCCT
     2401 GGAAGAGTCT GCTGTGAACA TGCTGCGCAG GGTCACTAAG ATGGTCCAAA CTGCCTTCGT
     2461 AAAGATTTTA TCAAATGATT TTGGTGATTT TTTAAACCTC TGCTGTACTG CTAAGTCCAA
     2521 AATTAAAGAA ATAAGATTAA ACCAAGAAAA GGAAGCTGAA AACCTGATCC GACTTCACTT
     2581 CCAGATGGAA CAGATTGTCT ACTGCCAGGA CCAGGTTTAC AAGGAAACCT TGAAGACGAT
     2641 CAGAGAGAAG GAAGCTGAGA AAGAGAAGAC CAAGGCATTA ATAAACCCTG CTACCTTTCA
     2701 AAATAACTCT CAGTTTCCTC AAAAGGGGTT GACTACCACT GAGATGACCC AGCACCTGAA
     2761 AGCCTACTAC CAGGAGTGCA GACGGAATAT TGGGAGACAG ATCCCTCTGA TTATCCAGTA
     2821 CTTCATCCTG AAAACATTTG GGGAGGAAAT AGAGAAAATG ATGCTTCAGC TTTTACAGGA
     2881 CACCAGTAAG TGCAGCTGGT TCCTGGAGGA GCAGAGTGAC ACCAGAGAGA AGAAGAAGTT
     2941 CCTGAAAAGG CGGCTTTTAA GGCTGGATGA GGCTCGGCAG AAGCTTGCCA AATTCTCCGA
     3001 TGGGGGAGGC GGATCCGGTG GAGCTTCTAG AATCCTCTGG CATGAGATGT GGCATGAAGG
     3061 CCTGGAAGAG GCATCTCGTT TGTACTTTGG GGAAAGGAAC GTGAAAGGCA TGTTTGAGGT
     3121 GCTGGAGCCC TTGCATGCTA TGATGGAACG GGGCCCCCAG ACTCTGAAGG AAACATCCTT
     3181 TAATCAGGCC TATGGTCGAG ATTTAATGGA GGCCCAAGAG TGGTGCAGGA AGTACATGAA
     3241 ATCAGGGAAT GTCAAGGACC TCCTCCAAGC CTGGGACCTC TATTATCATG TGTTCCGACG
     3301 AATCTCAAAG ACTAGTTAAA CGCGTTCGAG GAATTCACTC CTCAGGTGCA GGCTGCCTAT
     3361 CAGAAGGTGG TGGCTGGTGT GGCCAATGCC CTGGCTCACA AATACCACTG AGATCTTTTT
     3421 CCCTCTGCCA AAAATTATGG GGACATCATG AAGCCCCTTG AGCATCTGAC TTCTGGCTAA
     3481 TAAAGGAAAT TTATTTTCAT TGCAATAGTG TGTTGGAATT TTTTGTGTCT CTCACTCGGA
     3541 AGGACATATG GGAGGGCAAA TCATTTAAAA CATCAGAATG AGTATTTGGT TTAGAGTTTG
     3601 GCAACATATG CCATATGCTG GCTGCCATGA ACAAAGGTGG CTATAAAGAG GTCATCAGTA
     3661 TATGAAACAG CCCCCTGCTG TCCATTCCTT ATTCCATAGA AAAGCCTTGA CTTGAGGTTA
     3721 GATTTTTTTT ATATTTTGTT TTGTGTTATT TTTTTCTTTA ACATCCCTAA AATTTTCCTT
     3781 ACATGTTTTA CTAGCCAGAT TTTTCCTCCT CTCCTGACTA CTCCCAGTCA TAGCTGTCCC
     3841 TCTTCTCTTA TGAAGATCNN NNCTCGACCT GCAGCCCAAG CTTGGCGTAA TCATGGTCAT
     3901 AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA
     3961 GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC
     4021 GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GNNNNNNNNN NNGGATCCNA
     4081 TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA
     4141 CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG
     4201 AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG
     4261 GCCTAGGCTT TTGCAAAAAG CTNAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA
     4321 GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT
     4381 TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGATCCN CTGCATTAAT GAATCGGCCA
     4441 ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC
     4501 GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG
     4561 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA
     4621 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA
     4681 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG
     4741 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT
     4801 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG
     4861 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC
     4921 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT
     4981 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA
     5041 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC
     5101 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC
     5161 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT
     5221 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC
     5281 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT
     5341 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA
     5401 AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT
     5461 ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG
     5521 CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA
     5581 TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT
     5641 ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT
     5701 TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT
     5761 TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT
     5821 GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC
     5881 CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC
     5941 CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT
     6001 GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG
     6061 AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT
     6121 ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC
     6181 TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA
     6241 GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG
     6301 AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA
     6361 TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGNNN NNGTCGACAT
     6421 TGATTATTGA CTAGTTATTA ATAGTAATCA ATTACGGGGT CATTAGTTCA TAGCCCATAT
     6481 ATGGAGTTCC GCGTTACATA ACTTACGGTA AATGGCCCGC CTGGCTGACC GCCCAACGAC
     6541 CCCCGCCCAT TGACGTCAAT AATGACGTAT GTTCCCATAG TAACGCCAAT AGGGACTTTC
     6601 CATTGACGTC AATGGGTGGA CTATTTACGG TAAACTGCCC ACTTGGCAGT ACATCAAGTG
     6661 TATCATATGC CAAGTACGCC CCCTATTGAC GTCAATGACG GTAAATGGCC CGCCTGGCAT
     6721 TATGCCCAGT ACATGACCTT ATGGGACTTT CCTACTTGGC AGTACATCTA CGTATTAGTC
     6781 ATCGCTATTA CCATGGNNNN NGTCGAGGTG AGCCCCACGT TCTGCTTCAC TCTCCCCATC
     6841 TCCCCCCCCT CCCCACCCCC AATTTTGTAT TTATTTATTT TTTAATTATT TTGTGCAGCG
     6901 ATGGGGGCGG GGGGGGGGGG GGCGCGCGCC AGGCGGGGCG GGGCGGGGCG AGGGGCGGGG
     6961 CGGGGCGAGG CGGAGAGGTG CGGCGGCAGC CAATCAGAGC GGCGCGCTCC GAAAGTTTCC
     7021 TTTTATGGCG AGGCGGCGGC GGCGGCGGCC CT
//

