LOCUS       pCAXL-Mx1dL4_A2G      6629 bp    DNA     circular UNA 21-FEB-2012
DEFINITION  LMBP 9651.
ACCESSION   pCAXL-Mx1_A2G
SOURCE      Saccharomyces cerevisiae (A/Victoria/3/1975(H3N2)
  ORGANISM  Saccharomyces cerevisiae
            Saccharomyces cerevisiae
            Eukaryota; Fungi; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
FEATURES             Location/Qualifiers
     promoter        join(6382..6629,1..1087)
                     /note="Oriinal location: 3484..4818"
                     /label=AG
     CDS             1109..2884
                     /note="NM_010846"
                     /label="A2G Mx1"
     polyA_signal    2989..3080
                     /label="b-globin polyA"
     3'UTR           3081..3437
                     /label="3' FR of b-globin"
     promoter        3516..3599
                     /label="Lac promoter"
     rep_origin      complement(4043..4453)
                     /note="Oriinal location: complement(1145..1555)"
                     /label=ORI
     CDS             complement(4996..5853)
                     /note="Oriinal location: complement(2098..2955)"
                     /note="ORF: Frame #1   Start: atg (2098)   Stop:  taa
                     (2955)"
                     /label="Amp R"
     promoter        5993..6370
                     /label="hCMV-IE enhancer"
ORIGIN
        1 ATAAAAAGCG AAGCGCGCGG CGGGCGGGAG TCGCTGCGTT GCCTTCGCCC CGTGCCCCGC
       61 TCCGCGCCGC CTCGCGCCGC CCGCCCCGGC TCTGACTGAC CGCGTTACTC CCACAGGTGA
      121 GCGGGCGGGA CGGCCCTTCT CCTCCGGGCT GTAATTAGCG CTTGGTTTAA TGACGGCTCG
      181 TTTCTTTTCT GTGGCTGCGT GAAAGCCTTA AAGGGCTCCG GGAGGGCCCT TTGTGCGGGG
      241 GGGAGCGGCT CGGGGGGTGC GTGCGTGTGT GTGTGCGTGG GGAGCGCCGC GTGCGGCCCG
      301 CGCTGCCCGG CGGCTGTGAG CGCTGCGGGC GCGGCGCGGG GCTTTGTGCG CTCCGCGTGT
      361 GCGCGAGGGG AGCGCGGCCG GGGGCGGTGC CCCGCGGTGC GGGGGGGCTG CGAGGGGAAC
      421 AAAGGCTGCG TGCGGGGTGT GTGCGTGGGG GGGTGAGCAG GGGGTGTGGG CGCGGCGGTC
      481 GGGCTGTAAC CCCCCCCTGC ACCCCCCTCC CCGAGTTGCT GAGCACGGCC CGGCTTCGGG
      541 TGCGGGGCTC CGTGCGGGGC GTGGCGCGGG GCTCGCCGTG CCGGGCGGGG GGTGGCGGCA
      601 GGTGGGGGTG CCGGGCGGGG CGGGGCCGCC TCGGGCCGGG GAGGGCTCGG GGGAGGGGCG
      661 CGGCGGCCCC GGAGCGCCGG CGGCTGTCGA GGCGCGGCGA GCCGCAGCCA TTGCCTTTTA
      721 TGGTAATCGT GCGAGAGGGC GCAGGGACTT CCTTTGTCCC AAATCTGGCG GAGCCGAAAT
      781 CTGGGAGGCG CCGCCGCACC CCCTCTAGCG GGCGCGGGCG AAGCGGTGCG GCGCCGGCAG
      841 GAAGGAAATG GGCGGGGAGG GCCTTCGTGC GTCGCCGCGC CGCCGTCCCC TTCTCCATCT
      901 CCAGCCTCGG GGCTGCCGCA GGGGGACGGC TGCCTTCGGG GGGGACGGGG CAGGGCGGGG
      961 TTCGGCTTCT GGCGTGTGAC CGGCGGGNTC TAGANCCTCT GCTAACCATG TTCATGCCTT
     1021 CTTCTTTTTC CTACAGCTCC TGGGCAACGT GCTGGTTATT GTGCTGTCTC ATCATTTTGG
     1081 CAAAGAATTC CTCGAGAATT CGATTACGAT GGATTCTGTG AATAATCTGT GCAGGCACTA
     1141 TGAGGAGAAG GTGCGGCCCT GTATTGACCT CATCGACACC CTGAGGGCTC TGGGTGTGGA
     1201 GCAGGACCTG GCCCTGCCTG CCATCGCTGT CATTGGGGAC CAGAGTTCAG GGAAGAGCTC
     1261 TGTTCTGGAA GCACTGTCTG GAGTGGCCCT CCCCAGAGGC AGTGGTATTG TCACCAGATG
     1321 CCCTCTGGTG CTGAAATTGA GGAAGCTGAA AGAAGGAGAG GAGTGGAGAG GCAAAGTCTC
     1381 CTATGATGAC ATCGAAGTGG AGCTCTCTGA TCCTTCAGAG GTGGAAGAGG CCATTAACAA
     1441 GGGTCAGAAC TTCATTGCTG GGGTAGGCCT GGGGATCAGT GATAAGCTCA TTAGCCTGGA
     1501 TGTCAGCTCC CCAAATGTCC CAGACCTGAC TCTCATTGAC CTGCCTGGAA TTACCAGGGT
     1561 GGCTGTAGGC AACCAGCCTG CAGACATAGG ACGCCAGATC AAGAGACTTA TCAAGACATA
     1621 CATCCAAAAA CAAGAGACCA TCAACCTGGT GGTAGTCCCC AGCAATGTGG ACATTGCTAC
     1681 CACAGAGGCT CTGAGCATGG CTCAGGAGGT GGACCCTGAA GGGGATAGGA CCATAGGGGT
     1741 CTTGACCAAG CCTGATCTGG TGGACAGAGG TGCTGAAGGC AAGGTCTTGG ATGTGATGCG
     1801 GAACCTGGTG TATCCTCTGA AGAAGGGCTA CATGATAGTC AAGTGCAGAG GTCAGCAGGA
     1861 CATCCAAGAG CAGCTGAGCC TGACTGAGGC TTTTCAGAAA GAGCAAGTCT TCTTCAAGGA
     1921 TCACTCATAC TTCAGCATTC TTCTGGAAGA TGGGAAGGCC ACAGTGCCCT GCCTGGCAGA
     1981 GAGACTGACT GAGGAGCTCA CCTCCCACAT CTGTAAATCA CTGCCACTAT TGGAAGATCA
     2041 AATAAATAGC AGTCATCAGA GTGCAAGCGA GGAGCTGCAG AAGTACGGTG CAGACATACC
     2101 AGAAGATGAC AGAACGAGGA TGTCCTTTCT GGTGAATAAA ATCAGTGCCT TCAATAGGAA
     2161 TATCATGAAT TTGATACAAG CACAGGAAAC CGTATCAGAG GGAGACAGCC GGTTGTTTAC
     2221 CAAACTGCGA AATGAGTTTC TTGCTTGGGA TGATCATATT GAGGAATATT TTAAAAAAGA
     2281 TTCTCCTGAG GTACAAAGCA AGATGAAAGA ATTTGAAAAT CAGTATCGTG GCCGGGAGCT
     2341 GCCAGGGTTT GTGGACTACA AGGCATTTGA GAGCATCATC AAAAAGCGAG TCAAAGCCCT
     2401 GGAAGAGTCT GCTGTGAACA TGCTGCGCAG GGTCACTAAG ATGGTCCAAA CTGCCTTCGT
     2461 AAAGATTTTA TCAAATGATT TTGGTGATTT TTTAAACCTC TGCTGTACTG CTAAGTCCAA
     2521 AATTAAAGAA ATAAGATTAA ACCAAGAAAA GGAAGCTGAA AACCTGATCC GACTTCACTT
     2581 CCAGATGGAA CAGATTGTCT ACAAGGGGTT GACTACCACT GAGATGACCC AGCACCTGAA
     2641 AGCCTACTAC CAGGAGTGCA GACGGAATAT TGGGAGACAG ATCCCTCTGA TTATCCAGTA
     2701 CTTCATCCTG AAAACATTTG GGGAGGAAAT AGAGAAAATG ATGCTTCAGC TTTTACAGGA
     2761 CACCAGTAAG TGCAGCTGGT TCCTGGAGGA GCAGAGTGAC ACCAGAGAGA AGAAGAAGTT
     2821 CCTGAAAAGG CGGCTTTTAA GGCTGGATGA GGCTCGGCAG AAGCTTGCCA AATTCTCCGA
     2881 TTAAATTTGC GGCCGCACGC GTTCGAGGAA TTCACTCCTC AGGTGCAGGC TGCCTATCAG
     2941 AAGGTGGTGG CTGGTGTGGC CAATGCCCTG GCTCACAAAT ACCACTGAGA TCTTTTTCCC
     3001 TCTGCCAAAA ATTATGGGGA CATCATGAAG CCCCTTGAGC ATCTGACTTC TGGCTAATAA
     3061 AGGAAATTTA TTTTCATTGC AATAGTGTGT TGGAATTTTT TGTGTCTCTC ACTCGGAAGG
     3121 ACATATGGGA GGGCAAATCA TTTAAAACAT CAGAATGAGT ATTTGGTTTA GAGTTTGGCA
     3181 ACATATGCCA TATGCTGGCT GCCATGAACA AAGGTGGCTA TAAAGAGGTC ATCAGTATAT
     3241 GAAACAGCCC CCTGCTGTCC ATTCCTTATT CCATAGAAAA GCCTTGACTT GAGGTTAGAT
     3301 TTTTTTTATA TTTTGTTTTG TGTTATTTTT TTCTTTAACA TCCCTAAAAT TTTCCTTACA
     3361 TGTTTTACTA GCCAGATTTT TCCTCCTCTC CTGACTACTC CCAGTCATAG CTGTCCCTCT
     3421 TCTCTTATGA AGATCNNNNC TCGACCTGCA GCCCAAGCTT GGCGTAATCA TGGTCATAGC
     3481 TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA
     3541 TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT
     3601 CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGNN NNNNNNNNNG GATCCNATGC
     3661 ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC
     3721 CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG
     3781 CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC
     3841 TAGGCTTTTG CAAAAAGCTN AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA
     3901 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT
     3961 CCAAACTCAT CAATGTATCT TATCATGTCT GGATCCNCTG CATTAATGAA TCGGCCAACG
     4021 CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT
     4081 GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT
     4141 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC
     4201 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA
     4261 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA
     4321 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC
     4381 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG
     4441 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC
     4501 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG
     4561 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT
     4621 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT
     4681 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG
     4741 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC
     4801 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA
     4861 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC
     4921 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC
     4981 TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT
     5041 TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT
     5101 ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT
     5161 ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC
     5221 CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA
     5281 TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG
     5341 TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT
     5401 GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC
     5461 AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT
     5521 AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG
     5581 GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC
     5641 TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC
     5701 GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT
     5761 TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG
     5821 AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG
     5881 CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA
     5941 ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGNNNNNG TCGACATTGA
     6001 TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG
     6061 GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC
     6121 CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT
     6181 TGACGTCAAT GGGTGGACTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT
     6241 CATATGCCAA GTACGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT
     6301 GCCCAGTACA TGACCTTATG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC
     6361 GCTATTACCA TGGNNNNNGT CGAGGTGAGC CCCACGTTCT GCTTCACTCT CCCCATCTCC
     6421 CCCCCCTCCC CACCCCCAAT TTTGTATTTA TTTATTTTTT AATTATTTTG TGCAGCGATG
     6481 GGGGCGGGGG GGGGGGGGGC GCGCGCCAGG CGGGGCGGGG CGGGGCGAGG GGCGGGGCGG
     6541 GGCGAGGCGG AGAGGTGCGG CGGCAGCCAA TCAGAGCGGC GCGCTCCGAA AGTTTCCTTT
     6601 TATGGCGAGG CGGCGGCGGC GGCGGCCCT
//

