LOCUS       pCAXL-Mx1-GTPaseS      6735 bp    DNA     circular UNA 21-FEB-2012
DEFINITION  LMBP 9655.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES             Location/Qualifiers
     promoter        join(6488..6735,1..1087)
                     /label=AG
     CDS             1100..2995
                     /label=Mx1A2G-GTPaseS
     polyA_signal    3095..3186
                     /label="b-globin polyA"
     3'UTR           3187..3543
                     /label="3' FR of b-globin"
     promoter        3622..3705
                     /label="Lac promoter"
     rep_origin      complement(4149..4559)
                     /label=ORI
     CDS             complement(5102..5959)
                     /note="ORF: Frame #1   Start: atg (2098)   Stop:  taa
                     (2955)"
                     /label="Amp R"
     promoter        6099..6476
                     /label="hCMV-IE enhancer"
ORIGIN
        1 ATAAAAAGCG AAGCGCGCGG CGGGCGGGAG TCGCTGCGTT GCCTTCGCCC CGTGCCCCGC
       61 TCCGCGCCGC CTCGCGCCGC CCGCCCCGGC TCTGACTGAC CGCGTTACTC CCACAGGTGA
      121 GCGGGCGGGA CGGCCCTTCT CCTCCGGGCT GTAATTAGCG CTTGGTTTAA TGACGGCTCG
      181 TTTCTTTTCT GTGGCTGCGT GAAAGCCTTA AAGGGCTCCG GGAGGGCCCT TTGTGCGGGG
      241 GGGAGCGGCT CGGGGGGTGC GTGCGTGTGT GTGTGCGTGG GGAGCGCCGC GTGCGGCCCG
      301 CGCTGCCCGG CGGCTGTGAG CGCTGCGGGC GCGGCGCGGG GCTTTGTGCG CTCCGCGTGT
      361 GCGCGAGGGG AGCGCGGCCG GGGGCGGTGC CCCGCGGTGC GGGGGGGCTG CGAGGGGAAC
      421 AAAGGCTGCG TGCGGGGTGT GTGCGTGGGG GGGTGAGCAG GGGGTGTGGG CGCGGCGGTC
      481 GGGCTGTAAC CCCCCCCTGC ACCCCCCTCC CCGAGTTGCT GAGCACGGCC CGGCTTCGGG
      541 TGCGGGGCTC CGTGCGGGGC GTGGCGCGGG GCTCGCCGTG CCGGGCGGGG GGTGGCGGCA
      601 GGTGGGGGTG CCGGGCGGGG CGGGGCCGCC TCGGGCCGGG GAGGGCTCGG GGGAGGGGCG
      661 CGGCGGCCCC GGAGCGCCGG CGGCTGTCGA GGCGCGGCGA GCCGCAGCCA TTGCCTTTTA
      721 TGGTAATCGT GCGAGAGGGC GCAGGGACTT CCTTTGTCCC AAATCTGGCG GAGCCGAAAT
      781 CTGGGAGGCG CCGCCGCACC CCCTCTAGCG GGCGCGGGCG AAGCGGTGCG GCGCCGGCAG
      841 GAAGGAAATG GGCGGGGAGG GCCTTCGTGC GTCGCCGCGC CGCCGTCCCC TTCTCCATCT
      901 CCAGCCTCGG GGCTGCCGCA GGGGGACGGC TGCCTTCGGG GGGGACGGGG CAGGGCGGGG
      961 TTCGGCTTCT GGCGTGTGAC CGGCGGGNTC TAGANCCTCT GCTAACCATG TTCATGCCTT
     1021 CTTCTTTTTC CTACAGCTCC TGGGCAACGT GCTGGTTATT GTGCTGTCTC ATCATTTTGG
     1081 CAAAGAATTC CTCGAGACGA TGGATTCTGT GAATAATCTG TGCAGGCACT ATGAGGAGAA
     1141 GGTGCGGCCC TGTATTGACC TCATCGACAC CCTGAGGGCT CTGGGTGTGG AGCAGGACCT
     1201 GGCCCTGCCT GCCATCGCTG TCATTGGGGA CCAGAGTTCA GGGAAGAGCT CTGTGCTGGA
     1261 AGCACTGTCT GGAGTGGCCC TCCCCAGAGG CAGTGGTATT GTCACCAGAT GCCCTCTGGT
     1321 GCTGAAATTG AGGAAGCTGA ATGAAGGAGA GGAATGGAAA GGCAAAGTCT CCTATGATGA
     1381 CATCGAAGTG GAGCTCTCTG ATCCTTCAGA GGTGGAAGAG GCCATCAACA AGGGTCAAAA
     1441 CTTCATTGCT GGGGTAGGTC TGGGGATCAG TGATAAGCTC ATTAGCCTGG ATGTCAGCTC
     1501 CCCAAATGTC CCAGACCTGA CTCTCATTGA CCTGCCTGGA ATTACCAGGG TGCCTGTAGG
     1561 CAACCAGCCT GCAGACATAG GACGCCAGAT CAAGAGACTC ATCAAGACAT ACATCCAAAA
     1621 ACAAGAGACA ATCAACCTGG TGGTGGTCCC CAGCAATGTG GACATTGCCA CCACAGAGGC
     1681 TCTGAGCATG GCTCAGGAGG TGGACCCTGA AGGGGATAGG ACCATAGGGG TCTTGACCAA
     1741 GCCCGATCTG GTGGACAGAG GTGCTGAAGG CAAGGTCGTG GATGTGGTGC AGAACCTGGT
     1801 GTATCATCTG AAGAAGGGCT ATATGATAGT CAAGTGCAGG GGTCAGCAGG ACATCCAGGA
     1861 TCAGCTGAGC CTGACTGAAG CTCTTCAGAA TGAGCAAGTC TTCTTCCAGG AGCACTCATA
     1921 CTTCAGCATT CTTCTGGAAG ATGGGAAGGC CACAGTGCCC TGCCTGGCAA AGAGACTGAC
     1981 TGAGGAGCTC ACCTCCCACA TCTGTAAATC ACTGCCACTA TTGGAAGATC AAATAAATAG
     2041 CAGTCATCAG AGTGCAAGCG AGGAGCTGCA GAAGTACGGT GCAGACATAC CAGAAGATGA
     2101 CAGAACGAGG ATGTCCTTTC TGGTGAATAA AATCAGTGCC TTCAATAGGA ATATCATGAA
     2161 TTTGATACAA GCACAGGAAA CCGTATCAGA GGGAGACAGC CGGTTGTTTA CCAAACTGCG
     2221 AAATGAGTTT CTTGCTTGGG ATGATCATAT TGAGGAATAT TTTAAAAAAG ATTCTCCTGA
     2281 GGTACAAAGC AAGATGAAAG AATTTGAAAA TCAGTATCGT GGCCGGGAGC TGCCAGGGTT
     2341 TGTGGACTAC AAGGCATTTG AGAGCATCAT CAAAAAGCGA GTCAAAGCCC TGGAAGAGTC
     2401 TGCTGTGAAC ATGCTGCGCA GGGTCACTAA GATGGTCCAA ACTGCCTTCG TAAAGATTTT
     2461 ATCAAATGAT TTTGGTGATT TTTTAAACCT CTGCTGTACT GCTAAGTCCA AAATTAAAGA
     2521 AATAAGATTA AACCAAGAAA AGGAAGCTGA AAACCTGATC CGACTTCACT TCCAGATGGA
     2581 ACAGATTGTC TACTGCCAGG ACCAGGTTTA CAAGGAAACC TTGAAGACGA TCAGAGAGAA
     2641 GGAAGCTGAG AAAGAGAAGA CCAAGGCATT AATAAACCCT GCTACCTTTC AAAATAACTC
     2701 TCAGTTTCCT CAAAAGGGGT TGACTACCAC TGAGATGACC CAGCACCTGA AAGCCTACTA
     2761 CCAGGAGTGC AGACGGAATA TTGGGAGACA GATCCCTCTG ATTATCCAGT ACTTCATCCT
     2821 GAAAACATTT GGGGAGGAAA TAGAGAAAAT GATGCTTCAG CTTTTACAGG ACACCAGTAA
     2881 GTGCAGCTGG TTCCTGGAGG AGCAGAGTGA CACCAGAGAG AAGAAGAAGT TCCTGAAAAG
     2941 GCGGCTTTTA AGGCTGGATG AGGCTCGGCA GAAGCTTGCC AAATTCTCCG ATTAACCAGG
     3001 CTACGCGTTC GAGGAATTCA CTCCTCAGGT GCAGGCTGCC TATCAGAAGG TGGTGGCTGG
     3061 TGTGGCCAAT GCCCTGGCTC ACAAATACCA CTGAGATCTT TTTCCCTCTG CCAAAAATTA
     3121 TGGGGACATC ATGAAGCCCC TTGAGCATCT GACTTCTGGC TAATAAAGGA AATTTATTTT
     3181 CATTGCAATA GTGTGTTGGA ATTTTTTGTG TCTCTCACTC GGAAGGACAT ATGGGAGGGC
     3241 AAATCATTTA AAACATCAGA ATGAGTATTT GGTTTAGAGT TTGGCAACAT ATGCCATATG
     3301 CTGGCTGCCA TGAACAAAGG TGGCTATAAA GAGGTCATCA GTATATGAAA CAGCCCCCTG
     3361 CTGTCCATTC CTTATTCCAT AGAAAAGCCT TGACTTGAGG TTAGATTTTT TTTATATTTT
     3421 GTTTTGTGTT ATTTTTTTCT TTAACATCCC TAAAATTTTC CTTACATGTT TTACTAGCCA
     3481 GATTTTTCCT CCTCTCCTGA CTACTCCCAG TCATAGCTGT CCCTCTTCTC TTATGAAGAT
     3541 CNNNNCTCGA CCTGCAGCCC AAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA
     3601 AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC
     3661 TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC
     3721 CAGTCGGGAA ACCTGTCGTG CCAGNNNNNN NNNNNGGATC CNATGCATCT CAATTAGTCA
     3781 GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC
     3841 CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCG
     3901 GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA
     3961 AAGCTNAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT
     4021 TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT
     4081 GTATCTTATC ATGTCTGGAT CCNCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG
     4141 GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC
     4201 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG
     4261 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA
     4321 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC
     4381 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC
     4441 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG
     4501 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT
     4561 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC
     4621 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC
     4681 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG
     4741 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG
     4801 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA
     4861 CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG
     4921 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT
     4981 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA
     5041 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT
     5101 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG
     5161 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA
     5221 GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC
     5281 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT
     5341 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG
     5401 TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA
     5461 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG
     5521 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA
     5581 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG
     5641 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT
     5701 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA
     5761 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA
     5821 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG
     5881 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC
     5941 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT
     6001 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC
     6061 CGCGCACATT TCCCCGAAAA GTGCCACCTG NNNNNGTCGA CATTGATTAT TGACTAGTTA
     6121 TTAATAGTAA TCAATTACGG GGTCATTAGT TCATAGCCCA TATATGGAGT TCCGCGTTAC
     6181 ATAACTTACG GTAAATGGCC CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC
     6241 AATAATGACG TATGTTCCCA TAGTAACGCC AATAGGGACT TTCCATTGAC GTCAATGGGT
     6301 GGACTATTTA CGGTAAACTG CCCACTTGGC AGTACATCAA GTGTATCATA TGCCAAGTAC
     6361 GCCCCCTATT GACGTCAATG ACGGTAAATG GCCCGCCTGG CATTATGCCC AGTACATGAC
     6421 CTTATGGGAC TTTCCTACTT GGCAGTACAT CTACGTATTA GTCATCGCTA TTACCATGGN
     6481 NNNNGTCGAG GTGAGCCCCA CGTTCTGCTT CACTCTCCCC ATCTCCCCCC CCTCCCCACC
     6541 CCCAATTTTG TATTTATTTA TTTTTTAATT ATTTTGTGCA GCGATGGGGG CGGGGGGGGG
     6601 GGGGGCGCGC GCCAGGCGGG GCGGGGCGGG GCGAGGGGCG GGGCGGGGCG AGGCGGAGAG
     6661 GTGCGGCGGC AGCCAATCAG AGCGGCGCGC TCCGAAAGTT TCCTTTTATG GCGAGGCGGC
     6721 GGCGGCGGCG GCCCT
//
