LOCUS       p416-YFP-N-for N        6075 bp    DNA     circular     18-JAN-2016
DEFINITION  LMBP 9733.
SOURCE      
  ORGANISM  
COMMENT     (800)357-3114 or http://www.informaxinc.com/
COMMENT     VNTDATE|-11068805|
COMMENT     VNTNAME|p416-YFP-N for N|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT       (CDocSetData 1 1 0 0 0 0 "proto011" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43) 
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COMMENT        (CStringList "BamHI" "EcoRI" "EcoRV" "HindIII" "NcoI" "SalI" "XbaI" 
COMMENT         "XhoI") (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") 
COMMENT        (CObList) 1 "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 
COMMENT        0 (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 606 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "HindIII" "aagctt" 2 0 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 2 623 0 2618 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "SalI" "gtcgac" 2 0 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 2792 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 2263 0 0 "")) 
COMMENT       (CObList 
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COMMENT            (CStringList) 1 1 1 1 "5011..5529") 
COMMENT        #9=(CFSignal (CObList) "AMP" 4 0 1 4501 5361 0 (CStringList) 
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COMMENT        #10=(CFSignal (CObList) "URA3" 4 0 0 417 1220 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "417..1220") 
COMMENT        #11=(CFSignal (CObList) "T7 promoter" 30 0 0 1977 1993 0 (CStringList) 
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COMMENT        #12=(CFSignal (CObList) "CYC1 terminator" 43 0 1 2014 2261 0 
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COMMENT        #13=(CFSignal (CObList) "T3 promoter" 30 0 1 3303 3319 0 (CStringList) 
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COMMENT        #14=(CFSignal (CObList) "lac promoter" 30 0 1 3383 3466 0 (CStringList) 
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COMMENT        #15=(CFSignal (CObList) "pMB1 ori" 33 0 0 3558 3960 0 (CStringList) 
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COMMENT        #17=(CFSignal (CObList) "ADH1 promoter" 29 0 1 2861 3264 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #18=(CFSignal (CObList) "yEVenus fragment" 4 0 1 2268 2790 0 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                          "Created: 01/18/16 02:20" 1) 
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COMMENT                          "Last Modified: 01/18/16 02:20" 1) 
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FEATURES             Location/Qualifiers
     rep_origin      5503..6021
                     /vntifkey="33"
                     /label=CEN6/ARSH4
     CDS             complement(4501..5361)
                     /vntifkey="4"
                     /label=AMP
     CDS             417..1220
                     /vntifkey="4"
                     /label=URA3
     promoter        1977..1993
                     /vntifkey="30"
                     /label=T7\promoter
     terminator      complement(2014..2261)
                     /vntifkey="43"
                     /label=CYC1\terminator
     promoter        complement(3303..3319)
                     /vntifkey="30"
                     /label=T3\promoter
     promoter        complement(3383..3466)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      3558..3960
                     /vntifkey="33"
                     /label=pMB1\ori
     rep_origin      complement(1351..1806)
                     /vntifkey="33"
                     /label=f1\ori
     promoter        complement(2861..3264)
                     /vntifkey="29"
                     /label=ADH1\promoter
     CDS             complement(2268..2790)
                     /vntifkey="4"
                     /label=yEVenus\fragment
BASE COUNT     1757 a      1261 c      1379 g      1666 t        12 others
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc 
      241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg 
      301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc 
      361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt 
      421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat 
      481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca 
      541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg 
      601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg 
      661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca 
      721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac 
      781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa 
      841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg 
      901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct 
      961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac 
     1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg 
     1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa 
     1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa 
     1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac 
     1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac 
     1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat 
     1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa 
     1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca 
     1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg 
     1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta 
     1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg 
     1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa 
     1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg 
     1801 gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg 
     1861 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg 
     1921 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat 
     1981 acgactcact atagggcgaa ttgggtaccg gccgcaaatt aaagccttcg agcgtcccaa 
     2041 aaccttctca agcaaggttt tcagtataat gttacatgcg tacacgcgtc tgtacagaaa 
     2101 aaaaagaaaa atttgaaata taaataacgt tcttaatact aacataacta taaaaaaata 
     2161 aatagggacc tagacttcag gttgtctaac tccttccttt tcggttagag cggatgtggg 
     2221 gggagggcgt gaatgtaagc gtgacataac taattacatg actcgagnnn cttcaatgtt 
     2281 gtgtctaatt ttgaagttag ctttgatacc attcttttgt ttgtcagcag tgatgtaaac 
     2341 attgtgagag ttatagttgt attccaattt gtgacctaaa atgttaccat cttctttaaa 
     2401 atcaatacct tttaattcga ttctattaac taaggtatca ccttcaaact tgacttcagc 
     2461 tctggtcttg tagttaccgt catctttgaa aaaaatagtt ctttcttgaa cataaccttc 
     2521 tggcatggca gacttgaaaa agtcatgttg tttcatatga tctgggtatc tagcaaaaca 
     2581 ttgcaaacca taacctaaag tagtgactaa ggttggccat ggaactggca atttaccagt 
     2641 agtacaaatc aattttaagg tcaatttacc gtaagtagca tcaccttcac cttcaccgga 
     2701 gacagaaaat ttgtgaccat taacatcacc atctaattca accaaaattg ggacaacacc 
     2761 agtgaataat tcttcacctt tagacatnnn tctagatata gttttttctc cttgacgtta 
     2821 aagtatagag gtatattaac aattttttgt tgatactnnn cttggagttg attgtatgct 
     2881 tggtatagct tgaaatattg tgcagaaaaa gaaacaagga agaaagggaa cgagaacaat 
     2941 gacgaggaaa caaaagatta ataattgcag gtctatttat acttgatagc aaagcggcaa 
     3001 acttttttta tttcaaattc aagtaactgg aaggaaggcc gtataccgtt gctcattaga 
     3061 gagtagtgtg cgtgaatgaa ggaaggaaaa agtttcgtgt gcttcgagat acccctcatc 
     3121 agctctggaa caacgacatc tgttggtgct gtctttgtcg ttaatttttt cctttagtgt 
     3181 cttccatcat tttttttgtc attgcggata tggtgagaca acaacggggg agagagaaaa 
     3241 gaaaaaaaaa gaaaagaagt tgcannncta atccgtactt cagagctcca gcttttgttc 
     3301 cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg 
     3361 aaattgttat ccgctcacaa ttccacacaa cataggagcc ggaagcataa agtgtaaagc 
     3421 ctggggtgcc taatgagtga ggtaactcac attaattgcg ttgcgctcac tgcccgcttt 
     3481 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 
     3541 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 
     3601 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 
     3661 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 
     3721 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 
     3781 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 
     3841 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 
     3901 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 
     3961 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 
     4021 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 
     4081 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 
     4141 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 
     4201 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 
     4261 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 
     4321 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 
     4381 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 
     4441 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 
     4501 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 
     4561 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 
     4621 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 
     4681 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 
     4741 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 
     4801 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 
     4861 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 
     4921 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 
     4981 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 
     5041 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 
     5101 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 
     5161 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 
     5221 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 
     5281 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 
     5341 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 
     5401 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 
     5461 tccgcgcaca tttccccgaa aagtgccacc tgggtccttt tcatcacgtg ctataaaaat 
     5521 aattataatt taaatttttt aatataaata tataaattaa aaatagaaag taaaaaaaga 
     5581 aattaaagaa aaaatagttt ttgttttccg aagatgtaaa agactctagg gggatcgcca 
     5641 acaaatacta ccttttatct tgctcttcct gctctcaggt attaatgccg aattgtttca 
     5701 tcttgtctgt gtagaagacc acacacgaaa atcctgtgat tttacatttt acttatcgtt 
     5761 aatcgaatgt atatctattt aatctgcttt tcttgtctaa taaatatata tgtaaagtac 
     5821 gctttttgtt gaaatttttt aaacctttgt ttattttttt ttcttcattc cgtaactctt 
     5881 ctaccttctt tatttacttt ctaaaatcca aatacaaaac ataaaaataa ataaacacag 
     5941 agtaaattcc caaattattc catcattaaa agatacgagg cgcgtgtaag ttacaggcaa 
     6001 gcgatccgtc ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca 
     6061 cgaggccctt tcgtc 
//
