LOCUS       p413-YFP-N_for_N      6639 bp    DNA     circular UNA 07-APR.-2023
DEFINITION  LMBP 9734.
COMMENT     Accession number: LMBP 9734
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 07-APR-2023
            NGS data implemented
FEATURES             Location/Qualifiers
     CDS             430..1092
                     /label=HIS3
     rep_origin      complement(1352..1807)
                     /label=f1\ori
     promoter        1974..1990
                     /label=T7\promoter
     terminator      complement(2011..2258)
                     /label=CYC1\terminator
     CDS             complement(2345..2861)
                     /label=yEVenus\fragment
     promoter        complement(2876..3279)
                     /label=ADH1\promoter
     rep_origin      complement(4232..4823)
                     /label=pMB1\ori
     CDS             complement(4994..5854)
                     /label=ampicillin\resistance
     rep_origin      complement(5985..6497)
                     /label=CEN6/ARSH4\ori
ORIGIN
        1 AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG
       61 GGGCTGGCTT AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC ATAAATTCCC
      121 GTTTTAAGAG CTTGGTGAGC GCTAGGAGTC ACTGCCAGGT ATCGTTTGAA CACGGCATTA
      181 GTCAGGGAAG TCATAACACA GTCCTTTCCC GCAATTTTCT TTTTCTATTA CTCTTGGCCT
      241 CCTCTAGTAC ACTCTATATT TTTTTATGCC TCGGTAATGA TTTTCATTTT TTTTTTCCAC
      301 CTAGCGGATG ACTCTTTTTT TTCTTAGCGA TTGGCATTAT CACATAATGA ATTATACATT
      361 ATATAAAGTA ATGTGATTTC TTCGAAGAAT ATACTAAAAA ATGAGCAGGC AAGATAAACG
      421 AAGGCAAAGA TGACAGAGCA GAAAGCCCTA GTAAAGCGTA TTACAAATGA AACCAAGATT
      481 CAGATTGCGA TCTCTTTAAA GGGTGGTCCC CTAGCGATAG AGCACTCGAT CTTCCCAGAA
      541 AAAGAGGCAG AAGCAGTAGC AGAACAGGCC ACACAATCGC AAGTGATTAA CGTCCACACA
      601 GGTATAGGGT TTCTGGACCA TATGATACAT GCTCTGGCCA AGCATTCCGG CTGGTCGCTA
      661 ATCGTTGAGT GCATTGGTGA CTTACACATA GACGACCATC ACACCACTGA AGACTGCGGG
      721 ATTGCTCTCG GTCAAGCTTT TAAAGAGGCC CTAGGGGCCG TGCGTGGAGT AAAAAGGTTT
      781 GGATCAGGAT TTGCGCCTTT GGATGAGGCA CTTTCCAGAG CGGTGGTAGA TCTTTCGAAC
      841 AGGCCGTACG CAGTTGTCGA ACTTGGTTTG CAAAGGGAGA AAGTAGGAGA TCTCTCTTGC
      901 GAGATGATCC CGCATTTTCT TGAAAGCTTT GCAGAGGCTA GCAGAATTAC CCTCCACGTT
      961 GATTGTCTGC GAGGCAAGAA TGATCATCAC CGTAGTGAGA GTGCGTTCAA GGCTCTTGCG
     1021 GTTGCCATAA GAGAAGCCAC CTCGCCCAAT GGTACCAACG ATGTTCCCTC CACCAAAGGT
     1081 GTTCTTATGT AGTGACACCG ATTATTTAAA GCTGCAGCAT ACGATATATA TACATGTGTA
     1141 TATATGTATA CCTATGAATG TCAGTAAGTA TGTATACGAA CAGTATGATA CTGAAGATGA
     1201 CAAGGTAATG CATCATTCTA TACGTGTCAT TCTGAACGAG GCGCGCTTTC CTTTTTTCTT
     1261 TTTGCTTTTT CTTTTTTTTT CTCTTGAACT CGACGGATCT ATGCGGTGTG AAATACCGCA
     1321 CAGATGCGTA AGGAGAAAAT ACCGCATCAG GAAATTGTAA GCGTTAATAT TTTGTTAAAA
     1381 TTCGCGTTAA ATTTTTGTTA AATCAGCTCA TTTTTTAACC AATAGGCCGA AATCGGCAAA
     1441 ATCCCTTATA AATCAAAAGA ATAGACCGAG ATAGGGTTGA GTGTTGTTCC AGTTTGGAAC
     1501 AAGAGTCCAC TATTAAAGAA CGTGGACTCC AACGTCAAAG GGCGAAAAAC CGTCTATCAG
     1561 GGCGATGGCC CACTACGTGA ACCATCACCC TAATCAAGTT TTTTGGGGTC GAGGTGCCGT
     1621 AAAGCACTAA ATCGGAACCC TAAAGGGAGC CCCCGATTTA GAGCTTGACG GGGAAAGCCG
     1681 GCGAACGTGG CGAGAAAGGA AGGGAAGAAA GCGAAAGGAG CGGGCGCTAG GGCGCTGGCA
     1741 AGTGTAGCGG TCACGCTGCG CGTAACCACC ACACCCGCCG CGCTTAATGC GCCGCTACAG
     1801 GGCGCGTCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT
     1861 CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA
     1921 CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAGCGC GCGTAATACG
     1981 ACTCACTATA GGGCGAATTG GGTACCGGCC GCAAATTAAA GCCTTCGAGC GTCCCAAAAC
     2041 CTTCTCAAGC AAGGTTTTCA GTATAATGTT ACATGCGTAC ACGCGTCTGT ACAGAAAAAA
     2101 AAGAAAAATT TGAAATATAA ATAACGTTCT TAATACTAAC ATAACTATAA AAAAATAAAT
     2161 AGGGACCTAG ACTTCAGGTT GTCTAACTCC TTCCTTTTCG GTTAGAGCGG ATGTGGGGGG
     2221 AGGGCGTGAA TGTAAGCGTG ACATAACTAA TTACATGACT CGAGGTCGAC GGTATCGATA
     2281 AGCTTGATAT CGAATTCCTG CAGCCCGGGG GATCCAGAAC CTCCACCTCC GGAGCCACCG
     2341 CCACCTTCAA TGTTGTGTCT AATTTTGAAG TTAGCTTTGA TACCATTCTT TTGTTTGTCA
     2401 GCAGTGATGT AAACATTGTG AGAGTTATAG TTGTATTCCA ATTTGTGACC TAAAATGTTA
     2461 CCATCTTCTT TAAAATCAAT ACCTTTTAAT TCGATTCTAT TAACTAAGGT ATCACCTTCA
     2521 AACTTGACTT CAGCTCTGGT CTTGTAGTTA CCGTCATCTT TGAAAAAAAT AGTTCTTTCT
     2581 TGAACATAAC CTTCTGGCAT GGCAGACTTG AAAAAGTCAT GTTGTTTCAT ATGATCTGGG
     2641 TATCTAGCAA AACATTGCAA ACCATAACCT AAAGTAGTGA CTAAGGTTGG CCATGGAACT
     2701 GGCAATTTAC CAGTAGTACA AATCAATTTT AAGGTCAATT TACCGTAAGT AGCATCACCT
     2761 TCACCTTCAC CGGAGACAGA AAATTTGTGA CCATTAACAT CACCATCTAA TTCAACCAAA
     2821 ATTGGGACAA CACCAGTGAA TAATTCTTCA CCTTTAGACA TACTAGAGCG GCCAGCTTGG
     2881 AGTTGATTGT ATGCTTGGTA TAGCTTGAAA TATTGTGCAG AAAAAGAAAC AAGGAAGAAA
     2941 GGGAACGAGA ACAATGACGA GGAAACAAAA GATTAATAAT TGCAGGTCTA TTTATACTTG
     3001 ATAGCAAGAC AGCAAACTTT TTTTTATTTC AAATTCAAGT AACTGGAAGG AAGGCCGTAT
     3061 ACCGTTGCTC ATTAGAGAGT AGTGTGCGTG AATGAAGGAA GGAAAAAGTT TCGTGTGCTT
     3121 CGAGATACCC CTCATCAGCT CTGGAACAAC GACATCTGTT GGTGCTGTCT TTGTCGTTAA
     3181 TTTTTTCCTT TAGTGTCTTC CATCATTTTT TTGTCATTGC GGATATGGTG AGACAACAAC
     3241 GGGGGAGAGA GAAAAGAAAA AAAAAGAAAA GAAGTTGCAT GCGCCTATTA TTACTTCAAT
     3301 AGATGGCAAA TGGAAAAAGG GTAGTGAAAC TTCGATATGA TGATGGCTAT CAAGTCTAGG
     3361 GCTACAGTAT TAGTTCGTTA TGTACCACCA TCAATGAGGC AGTGTAATTG GTGTAGTCTT
     3421 GTTTAGCCCA TTATGTCTTG TCTGGTATCT GTTCTATTGT ATATCTCCCC TCCGCCACCT
     3481 ACATGTTAGG GAGACCAACG AAGGTATTAT AGGAATCCCG ATGTATGGGT TTGGTTGCCA
     3541 GAAAAGAGGA AGTCCATATT GTACACCCGG AAACAACAAA AGGATATCCG AAATATTCCA
     3601 CGGTTTAGAA AAAAATCGGA AAAGAGCGCG GAGGGGTGTT ACCCCCCTTC TCTACTAGCA
     3661 TTGGACTTTA ATTAATATAT GTGCATAGGA GAAGTGTAAA GTTCCCTTCC ATATTGTAAC
     3721 ATAATAAAGT GCACACCCAA ATGAATTGAA AGCGTACTCA AACAGACAAC CATTTCCAGT
     3781 GTTGTATGTA CCTGTCTATT TATACTGGTA GCAACCCTAT TGCTGTTTCC TCTTCAAAGT
     3841 ACTCTAGCGG TTATGCGCGT CTCACCTTCA AGGTCATGGT CGCTCTATTG TTCGCACCAC
     3901 CGGCAAACTC GCGTCTCGCA AGTCTTGGCT CATTCTTCTA GTATACTCAT GTTGCAAATG
     3961 CACTCAGGTT CTTTCGGCAA CTTAAATAAT GACACCAGCT GCATTAATGA ATCGGCCAAC
     4021 GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC
     4081 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT
     4141 TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG
     4201 CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG
     4261 AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT
     4321 ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA
     4381 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT
     4441 GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC
     4501 CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA
     4561 GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG
     4621 TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG
     4681 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT
     4741 GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA
     4801 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC
     4861 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA
     4921 CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA
     4981 CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT
     5041 TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT
     5101 TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT
     5161 TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT
     5221 CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA
     5281 ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG
     5341 GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT
     5401 TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG
     5461 CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG
     5521 TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC
     5581 GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA
     5641 CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC
     5701 CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT
     5761 TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG
     5821 GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA
     5881 GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA
     5941 AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGGA TCGCTTGCCT
     6001 GTAACTTACA CGCGCCTCGT ATCTTTTAAT GATGGAATAA TTTGGGAATT TACTCTGTGT
     6061 TTATTTATTT TTATGTTTTG TATTTGGATT TTAGAAAGTA AATAAAGAAG GTAGAAGAGT
     6121 TACGGAATGA AGAAAAAAAA TAAACAAAGG TTTAAAAAAT TTCAACAAAA AGCGTACTTT
     6181 ACATATATAT TTATTAGACA AGAAAAGCAG ATTAAATAGA TATACATTCG ATTAACGATA
     6241 AGTAAAATGT AAAATCACAG GATTTTCGTG TGTGGTCTTC TACACAGACA AGATGAAACA
     6301 ATTCGGCATT AATACCTGAG AGCAGGAAGA GCAAGATAAA AGGTAGTATT TGTTGGCGAT
     6361 CCCCCTAGAG TCTTTTACAT CTTCGGAAAA CAAAAACTAT TTTTTCTTTA ATTTCTTTTT
     6421 TTACTTTCTA TTTTTAATTT ATATATTTAT ATTAAAAAAT TTAAATTATA ATTATTTTTA
     6481 TAGCACGTGA TGAAAAGGAC CCTAAGAAAC CATTATTATC ATGACATTAA CCTATAAAAA
     6541 TAGGCGTATC ACGAGGCCCT TTCGTCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG
     6601 ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTA
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