LOCUS       pBI-EGFP-clinker-ATG        4982 bp    DNA     circular      4-MAR-2016
DEFINITION  LMBP 9782.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|-11135186|
COMMENT     VNTNAME|pBI-EGFP-clinker-ATG|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                         "Created: 03/04/16 04:41" 1) 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "EGFP" "@N" 1 
COMMENT                                   -7.8151 10.454 0 -10 2.256 1.16519 #112#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #114=(CGroupWidget 
COMMENT                            (CWidget 29 (7 29 0) 1 2 0 0 Nil -537 100) 
COMMENT                            (CObjectList 
COMMENT                             #115=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #0# 0) 1 2 0 0 #84# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 8388736 0) 1 
COMMENT                                    5.65619 6.11957 1 0.082322) 0.8 1.8 0) 
COMMENT                             #116=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #87# 101704360 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "hCMVT promoter" 
COMMENT                                   "@N" 1 12.3606 8.71165 0 -10 6.7928 1.16519 
COMMENT                                   #115#) 
COMMENT                             #117=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #7# 0) 1 2 0 0 #84# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 8388736 0) 
COMMENT                                    0.835356 5.69397 6.1586 1 0.082322) 0.8 1.8 
COMMENT                                   1) 
COMMENT                             #118=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #87# 1413567572 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "hCMVT promoter" 
COMMENT                                   "@N" 1 10.1324 10.454 0 -10 6.7928 1.16519 
COMMENT                                   #117#)) (CObjectList)) 
COMMENT                      #119=(CGroupWidget 
COMMENT                            (CWidget 33 (7 33 0) 1 2 0 0 Nil -116 100) 
COMMENT                            (CObjectList 
COMMENT                             #120=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #2# 0) 1 2 0 0 #59# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                    3.59997 4.10741 1 0.082322) 0.8 1.8 1) 
COMMENT                             #121=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #87# 1195460436 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "pMB1 ori" "@N" 
COMMENT                                   1 10.6793 -8.71165 0 -10 3.56994 1.16519 
COMMENT                                   #120#)) (CObjectList)) 
COMMENT                      #122=(CGroupWidget 
COMMENT                            (CWidget 43 (7 43 0) 1 2 0 0 Nil -889 100) 
COMMENT                            (CObjectList 
COMMENT                             #123=(CLine 
COMMENT                                   (CWidget 0 (3 #1# 0) 1 2 0 0 #50# -2148 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 0.835356 4.62115 
COMMENT                                   4.737) 
COMMENT                             #124=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #97# 75677347 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "3' UTR of rabbit \337-globin, incl. polyA" 
COMMENT                                   "@N" 1 19.6681 0 0 -10 14.3046 1.16519 #123#) 
COMMENT                             #125=(CLine 
COMMENT                                   (CWidget 0 (3 #4# 0) 1 2 0 0 #50# 8 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 0.835356 1.06778 
COMMENT                                   1.18992) 
COMMENT                             #126=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #97# 1196708948 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 polyA early" "@N" 1 -15.2923 3.48466 0 
COMMENT                                   -10 7.11509 1.16519 #125#) 
COMMENT                             #127=(CLine 
COMMENT                                   (CWidget 0 (3 #5# 0) 1 2 0 0 #50# -2066 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 0.670712 1.06778 
COMMENT                                   1.18992) 
COMMENT                             #128=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #97# 1096042324 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 polyA late" "@N" 1 -14.4569 5.22699 0 
COMMENT                                   -10 6.54489 1.16519 #127#)) (CObjectList))) 
COMMENT                     (CObjectList)) 
COMMENT               #129=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -116 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #130=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil -889 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #131=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -585 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 6 (CObjectList) (CObList) 1 (CObList)) (CObList) 103233472 
COMMENT       (CStringList) 103240792 103241712 (CObList)))
FEATURES             Location/Qualifiers
     promoter        123..490
                     /vntifkey="29"
                     /label=hCMVT\promoter
     terminator      1221..1312
                     /vntifkey="43"
                     /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA
     rep_origin      complement(1721..2123)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(2664..3524)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     terminator      4038..4134
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(4038..4134)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     CDS             complement(4231..4950)
                     /vntifkey="4"
                     /label=EGFP
     promoter        complement(92..460)
                     /vntifkey="29"
                     /label=hCMVT\promoter
BASE COUNT     1183 a      1222 c      1219 g      1358 t 
ORIGIN
        1 ctgcaggaat tcggggccgc ggaggctgga tcggtcccgg tgtcttctat ggaggtcaaa 
       61 acagcgtgga tggcgtctcc aggcgatctg acggttcact aaacgagctc tgcttatata 
      121 ggtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc 
      181 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa 
      241 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac 
      301 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata 
      361 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga 
      421 gctcggtacc cgggtcgagt aggcgtgtac ggtgggaggc ctatataagc agagctcgtt 
      481 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac 
      541 accgggaccg atccagcctc cgcggccccg aattcgagct cggtacccgg ggatcctcta 
      601 gtcagctgtc cggatcgata cgtacacgtg ctcgagatgg accctcatga taattttgtt 
      661 tctttcactt tctactctgt tgacaaccat tgtctcctct tattttcttt tcattttctg 
      721 taactttttt cgttaaactt tagcttgcat ttgtaacgaa tttttaaatt cactttcgtt 
      781 tatttgtcag attgtaagta ctttctctaa tcactttttt ttcaaggcaa tcagggtaat 
      841 tatattgtac ttcagcacag ttttagagaa caattgttat aattaaatga taaggtagaa 
      901 tatttctgca tataaattct ggctggcgtg gaaatattct tattggtaga aacaactaca 
      961 tcctggtaat catcctgcct ttctctttat ggttacaatg atatacactg tttgagatga 
     1021 ggataaaata ctctgagtcc aaaccgggcc cctctgctaa ccatgttcat gccttcttct 
     1081 ttttcctaca gctcctgggc aacgtgctgg ttgttgtgct gtctcatcat tttggcaaag 
     1141 aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc 
     1201 tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga 
     1261 agccccttga gcatctgact tctgggtaat aaaggaaatt tattttcatt gcaatagtgt 
     1321 gtgggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac 
     1381 atcagaatga gtatttggtt tagagtttgg caacatatgc catatgctgg ctgccatgaa 
     1441 caaaggtggc tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta 
     1501 ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt 
     1561 ttttctttaa catccctaaa attttcctta catgttttac tagccagatt tttcctcctc 
     1621 tcctgactac tcccagtcat agctgtccct cttctcttat gaactcgact gcattaatga 
     1681 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc 
     1741 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 
     1801 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 
     1861 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 
     1921 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 
     1981 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 
     2041 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa 
     2101 tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 
     2161 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 
     2221 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 
     2281 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 
     2341 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 
     2401 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 
     2461 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 
     2521 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 
     2581 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 
     2641 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 
     2701 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 
     2761 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 
     2821 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 
     2881 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 
     2941 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc 
     3001 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 
     3061 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 
     3121 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 
     3181 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 
     3241 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 
     3301 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 
     3361 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 
     3421 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 
     3481 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 
     3541 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 
     3601 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc 
     3661 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt 
     3721 cgtcttcact cgacgctggt cgagctgata cttcccgtcc gccaggggac atgccggcga 
     3781 tgctgaaggt cgcgcgcatt cccgatgaag aggccggtta ccgcctgttg acctggtggg 
     3841 acgggcaggg cgccgcccga gtcttgcctc ggcggcgggc gctctgctca tggagcgcgc 
     3901 gtccggggac cttgcacaga tagcgtggtc cggccagacg acgaggcttg caggatcata 
     3961 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc 
     4021 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat 
     4081 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 
     4141 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcctctag 
     4201 aactaggtcg acaagcttga tatcgaattc ttacttgtac agctcgtcca tgccgagagt 
     4261 gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc 
     4321 tttgctcagg gcggactggg tgctcaggta gtggttgtcg ggcagcagca cggggccgtc 
     4381 gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt 
     4441 gtggcggatc ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca tgatatagac 
     4501 gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa 
     4561 gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc 
     4621 gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc 
     4681 gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca 
     4741 ctgcacgccg taggtcaggg tggtcacgag ggtgggccag ggcacgggca gcttgccggt 
     4801 ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga 
     4861 cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc 
     4921 ggtgaacagc tcctcgccct tgctcaccat ccgcggggat ccactagttc tagagcggcc 
     4981 gc 
//
