LOCUS       pCi-C-myc-DEST          5739 bp    DNA     circular     29-JAN-2016
DEFINITION  LMBP 9840.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|-11084431|
COMMENT     VNTNAME|pCi-C-myc-DEST|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
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COMMENT       (CMolPar 0 0 0 0 4294967295 1 5739 0 0 0 0 0 0 0 0) (CStringList) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (CObList) 
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COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
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COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #2=(CFSignal (CObList) "attR1" 0 0 1 1099 1223 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #3=(CFSignal (CObList) "lacUV5 promoter" 30 0 0 1227 1283 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #4=(CFSignal (CObList) "chloramphenicol resistance" 4 0 0 1332 1991 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #5=(CFSignal (CObList) "ccdB" 4 0 0 2312 2617 0 (CStringList) 
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COMMENT        #6=(CFSignal (CObList) "attR2" 0 0 0 2658 2782 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #7=(CFSignal (CObList) "SV40 polyA early" 43 0 1 2898 2994 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #8=(CFSignal (CObList) "SV40 polyA late" 43 0 0 2898 2994 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
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COMMENT        #10=(CFSignal (CObList) "ampicillin resistance" 4 0 0 4047 4907 0 
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COMMENT        #11=(CFSignal (CObList) "pMB1 ori" 33 0 0 5448 5726 0 (CStringList) 
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FEATURES             Location/Qualifiers
     promoter        138..721
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     promoter        1034..1050
                     /vntifkey="30"
                     /label=T7\promoter
     attenuator      complement(1099..1223)
                     /vntifkey="0"
                     /label=attR1
     promoter        1227..1283
                     /vntifkey="30"
                     /label=lacUV5\promoter
     CDS             1332..1991
                     /vntifkey="4"
                     /label=chloramphenicol\resistance
     CDS             2312..2617
                     /vntifkey="4"
                     /label=ccdB
     attenuator      2658..2782
                     /vntifkey="0"
                     /label=attR2
     terminator      complement(2898..2994)
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      2898..2994
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      3153..3608
                     /vntifkey="33"
                     /label=f1\ori
     CDS             4047..4907
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      5448..5726
                     /vntifkey="33"
                     /label=pMB1\ori
     misc_marker     2793..2822
                     /vntifkey="22"
                     /label=Myc\epitope
BASE COUNT     1503 a      1314 c      1369 g      1552 t         1 others
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta 
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg 
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc 
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc 
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat 
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc 
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa 
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac 
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta 
     1021 aggctagagt acttaatacg actcactata ggctagcctc gagaattcac gcgtggtacc 
     1081 tctagagtcg acccatcaac aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat 
     1141 ataaatatca atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa 
     1201 acacaacata tccagtcact atggcggccg cattaggcac cccaggcttt acactttatg 
     1261 cttccggctc gtataatgtg tggattttga gttaggatcc gtcgagattt tcaggagcta 
     1321 aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc 
     1381 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg 
     1441 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc 
     1501 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa 
     1561 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg 
     1621 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc 
     1681 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag 
     1741 ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg 
     1801 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt 
     1861 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg 
     1921 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg 
     1981 gcggggcgta aacgcgtgga tccggcttac taaaagccag ataacagtat gcgtatttgc 
     2041 gcgctcgcga accggtgtat acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg 
     2101 tattacagtg acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat 
     2161 atctccggtc tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc 
     2221 tggaaagcgg aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc 
     2281 tcttttgctg acgagaacag gggctggtga aatgcagttt aaggtttaca cctataaaag 
     2341 agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcccgggcg 
     2401 acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact 
     2461 ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag 
     2521 tgtgccggtc tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat 
     2581 caaaaacgcc attaacctga tgttctgggg aatataaatg tcaggctccc ttatacacag 
     2641 ccagtctgca ggtcgaccat agtgactgga tatgttgtgt tttacagtat tatgtagtct 
     2701 gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt 
     2761 cagctttctt gtacaaagtg gttgatgcat caatgcagaa gctgatctca gaggaggacc 
     2821 tgtaggggcg gccgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca 
     2881 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat 
     2941 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt 
     3001 ttcaggttca gggggagatg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg 
     3061 gtaaaatcga taaggatccg ggctggcgta atagcgaaga ggcccgcacc gatcgccctt 
     3121 cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg cattaagcgc 
     3181 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc 
     3241 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct 
     3301 aaatcggggg ctccctttag ggttccgatt tagagcttta cggcacctcg accgcaaaaa 
     3361 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc 
     3421 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact 
     3481 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg 
     3541 gttaaaaaat gagctgattt aacaaatatt taacgcgaat tttaacaaaa tattaacgtt 
     3601 tacaatttcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 
     3661 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc 
     3721 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac 
     3781 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac 
     3841 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa 
     3901 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt 
     3961 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc 
     4021 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc 
     4081 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa 
     4141 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg 
     4201 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag 
     4261 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc 
     4321 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta 
     4381 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg 
     4441 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca 
     4501 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac 
     4561 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat 
     4621 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg 
     4681 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata 
     4741 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta 
     4801 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa 
     4861 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag 
     4921 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg 
     4981 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact 
     5041 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 
     5101 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 
     5161 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 
     5221 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 
     5281 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 
     5341 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 
     5401 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 
     5461 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 
     5521 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 
     5581 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 
     5641 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 
     5701 ccttttgctg gccttttgct cacatggctc gacagatct 
//
