LOCUS       pCi-N-EGFP-DEST         6399 bp    DNA     circular     24-OCT-2017
DEFINITION  LMBP 9843.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 9843
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 24-OCT-2017
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11084431|
COMMENT     VNTDBDATE|-11997291|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCi-N-EGFP-DEST|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTREPLTYPE|Phage
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT        (CObList) -1 "") 
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COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT            "") #1=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 749 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "HpaI" "gttaac" 4 0 1 3633 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 3 514 0 1063 0 2547 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "NheI" "gctagc" 2 0 1 1053 0 0 "")) 
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COMMENT        #6=(CFSignal (CObList) "T7 promoter" 30 0 0 1034 1050 0 (CStringList) 
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COMMENT        #7=(CFSignal (CObList) "attR1" 0 0 1 1799 1923 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1799..1923)") 
COMMENT        #8=(CFSignal (CObList) "lacUV5 promoter" 30 0 0 1927 1983 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "1927..1983") 
COMMENT        #9=(CFSignal (CObList) "chloramphenicol resistance" 4 0 0 2032 2691 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "2032..2691") 
COMMENT        #10=(CFSignal (CObList) "ccdB" 4 0 0 3012 3317 0 (CStringList) 
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COMMENT        #11=(CFSignal (CObList) "attR2" 0 0 0 3358 3482 0 (CStringList) 
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COMMENT                          "DNA Plasmid Phage 'pCi-N-EGFP-DEST'" 1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 
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COMMENT                          "Created: 01/29/16 10:46" 1) 
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COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
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COMMENT                          "TEL: 0032-9-33-13-843  " 1) 
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COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.lmbp@UGent.be</a>" 
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FEATURES             Location/Qualifiers
     promoter        138..721
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     promoter        1034..1050
                     /vntifkey="30"
                     /label=T7\promoter
     attenuator      complement(1799..1923)
                     /vntifkey="0"
                     /label=attR1
     promoter        1927..1983
                     /vntifkey="30"
                     /label=lacUV5\promoter
     CDS             2032..2691
                     /vntifkey="4"
                     /label=chloramphenicol\resistance
     CDS             3012..3317
                     /vntifkey="4"
                     /label=ccdB
     attenuator      3358..3482
                     /vntifkey="0"
                     /label=attR2
     terminator      complement(3558..3654)
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      3558..3654
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      3813..4268
                     /vntifkey="33"
                     /label=f1\ori
     CDS             4707..5567
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      6108..6386
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             1064..1780
                     /vntifkey="4"
                     /label=EGFP
BASE COUNT     1658 a      1543 c      1556 g      1642 t 
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta 
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg 
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc 
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc 
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat 
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc 
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa 
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac 
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta 
     1021 aggctagagt acttaatacg actcactata ggctagctag accatggtga gcaagggcga 
     1081 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca 
     1141 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa 
     1201 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac 
     1261 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa 
     1321 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa 
     1381 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct 
     1441 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta 
     1501 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt 
     1561 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa 
     1621 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc 
     1681 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac 
     1741 cgccgccggg atcactctcg gcatggacga gctgtacaag ctctcgagtc gacccatcac 
     1801 aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa 
     1861 ttagattttg cataaaaaac agactacata atactgtaaa acacaacata tccagtcact 
     1921 atggcggccg cattaggcac cccaggcttt acactttatg cttccggctc gtataatgtg 
     1981 tggattttga gttaggatcc gtcgagattt tcaggagcta aggaagctaa aatggagaaa 
     2041 aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag 
     2101 gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc 
     2161 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt 
     2221 gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg 
     2281 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca 
     2341 tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat 
     2401 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt 
     2461 ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg 
     2521 gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg 
     2581 ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga 
     2641 atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta aacgcgtgga 
     2701 tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctcgcga accggtgtat 
     2761 acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg acagttgaca 
     2821 gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc tggtaagcac 
     2881 aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga 
     2941 agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg acgagaacag 
     3001 gggctggtga aatgcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt 
     3061 ttgtggatgt acagagtgat attattgaca cgcccgggcg acggatggtg atccccctgg 
     3121 ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg gtgcatatcg 
     3181 gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtc tccgttatcg 
     3241 gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga 
     3301 tgttctgggg aatataaatg tcaggctccc ttatacacag ccagtctgca ggtcgaccat 
     3361 agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa 
     3421 tttaatatat tgatatttat atcattttac gtttctcgtt cagctttctt gtacaaagtg 
     3481 gtgatgggcg gccgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca 
     3541 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat 
     3601 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt 
     3661 ttcaggttca gggggagatg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg 
     3721 gtaaaatcga taaggatccg ggctggcgta atagcgaaga ggcccgcacc gatcgccctt 
     3781 cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg cattaagcgc 
     3841 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc 
     3901 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct 
     3961 aaatcggggg ctccctttag ggttccgatt tagagcttta cggcacctcg accgcaaaaa 
     4021 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc 
     4081 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact 
     4141 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg 
     4201 gttaaaaaat gagctgattt aacaaatatt taacgcgaat tttaacaaaa tattaacgtt 
     4261 tacaatttcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 
     4321 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc 
     4381 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac 
     4441 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac 
     4501 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa 
     4561 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt 
     4621 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc 
     4681 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc 
     4741 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa 
     4801 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg 
     4861 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag 
     4921 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc 
     4981 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta 
     5041 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg 
     5101 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca 
     5161 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac 
     5221 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat 
     5281 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg 
     5341 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata 
     5401 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta 
     5461 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa 
     5521 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag 
     5581 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg 
     5641 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact 
     5701 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 
     5761 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 
     5821 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 
     5881 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 
     5941 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 
     6001 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 
     6061 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 
     6121 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 
     6181 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 
     6241 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 
     6301 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 
     6361 ccttttgctg gccttttgct cacatggctc gacagatct 
//
