LOCUS       pCD-F-NvCARD-CC           7629 bp    DNA     circular      9-OCT-2018
DEFINITION  LMBP 9854.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 10187
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 9-OCT-2018
            
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11195426|
COMMENT     VNTDBDATE|-12501412|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCD-F-NvCARD-CC|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTREPLTYPE|Phage
COMMENT     VNTREPLTYPE|Virus
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTEXTCHREPL|Animal/Other Eukaryotic
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCD-F-NvCARD-CC" 0 7629 
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COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "ApaI" "gggccc" 6 0 1 1 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "BglII" "agatct" 2 0 1 4444 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 5382 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 5 962 0 1697 0 5042 0 5350 
COMMENT            0 6511 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 1318 0 6997 0 0 "")) 
COMMENT       (CObList 
COMMENT        #5=(CFSignal (CObList) "ampicillin resistance" 4 0 1 3435 4295 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(3435..4296)") 
COMMENT        #6=(CFSignal (CObList) "SV40 polyA early" 43 0 0 2104 2178 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "1946..2185") 
COMMENT        #7=(CFSignal (CObList) "Tn5 neomycin resistance" 4 0 0 1136 1930 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "1136..1930") 
COMMENT        #8=(CFSignal (CObList) "f1 ori" 33 0 0 297 725 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "297..710") 
COMMENT        #9=(CFSignal (CObList) "BGH polyA" 43 0 0 27 251 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "20..234") 
COMMENT        #10=(CFSignal (CObList) "hCMV-IE promoter and enhancer" 29 0 0 4666 5249 
COMMENT             0 (CStringList) (CStringList) 1 1 1 1 "4640..5294") 
COMMENT        #11=(CFSignal (CObList) "NvCARMA" 4 0 0 5384 7624 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5384..7624") 
COMMENT        #12=(CFSignal (CObList) "T7 promoter" 30 0 0 5294 5310 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #13=(CFSignal (CObList) "FLAG epitope" 22 0 0 5354 5377 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #14=(CFSignal (CObList) "SV40 ori" 33 0 0 969 1044 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #15=(CFSignal (CObList) "SV40 early promoter" 29 0 0 730 1012 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #16=(CFSignal (CObList) "SV40 polyA late" 43 0 1 2104 2178 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #17=(CFSignal (CObList) "pMB1 ori" 33 0 1 2492 2894 0 (CStringList) 
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COMMENT                          "DNA Plasmid Phage Virus 'pCD-F-NvCARD-CC'" 1) 
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COMMENT                          1) 
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COMMENT                          "Local object" 1) 
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COMMENT                          "Created: 04/15/16 01:41" 1) 
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COMMENT                          "Last Modified: 10/09/18 12:07" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 7629 bp" 1) 
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COMMENT                          "Modification Date in the Original DB: 15-APR-2016" 1))) 
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COMMENT                          "Replication Origin (3 total)") 
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FEATURES             Location/Qualifiers
     CDS             complement(3435..4295)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     terminator      2104..2178
                     /vntifkey="43"
                     /label=SV40\polyA\early
     CDS             1136..1930
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     rep_origin      297..725
                     /vntifkey="33"
                     /label=f1\ori
     terminator      27..251
                     /vntifkey="43"
                     /label=BGH\polyA
     promoter        4666..5249
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     CDS             5384..7624
                     /vntifkey="4"
                     /label=NvCARMA
     promoter        5294..5310
                     /vntifkey="30"
                     /label=T7\promoter
     misc_marker     5354..5377
                     /vntifkey="22"
                     /label=FLAG\epitope
     rep_origin      969..1044
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        730..1012
                     /vntifkey="29"
                     /label=SV40\early\promoter
     terminator      complement(2104..2178)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(2492..2894)
                     /vntifkey="33"
                     /label=pMB1\ori
BASE COUNT     1970 a      1883 c      1974 g      1802 t 
ORIGIN
        1 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg 
       61 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata 
      121 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt 
      181 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt 
      241 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc 
      301 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac 
      361 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt 
      421 cgccggcttt ccccgtcaag ctctaaatcg gggcatccct ttagggttcc gatttagtgc 
      481 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc 
      541 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact 
      601 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg 
      661 gattttgggg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc 
      721 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccaggc 
      781 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc 
      841 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt 
      901 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc 
      961 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct 
     1021 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg 
     1081 agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat 
     1141 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta 
     1201 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca 
     1261 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga 
     1321 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga 
     1381 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct 
     1441 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg 
     1501 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga 
     1561 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca 
     1621 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga 
     1681 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg 
     1741 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc 
     1801 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt 
     1861 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga 
     1921 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca 
     1981 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc 
     2041 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac 
     2101 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 
     2161 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 
     2221 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag 
     2281 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc 
     2341 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc 
     2401 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa 
     2461 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg 
     2521 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 
     2581 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 
     2641 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 
     2701 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 
     2761 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 
     2821 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc 
     2881 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 
     2941 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 
     3001 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 
     3061 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 
     3121 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 
     3181 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 
     3241 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 
     3301 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 
     3361 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 
     3421 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 
     3481 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 
     3541 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 
     3601 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 
     3661 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 
     3721 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 
     3781 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 
     3841 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 
     3901 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 
     3961 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 
     4021 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 
     4081 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 
     4141 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 
     4201 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 
     4261 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 
     4321 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 
     4381 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg 
     4441 ggagatctcc cgatccccta tggtcgactc tcagtacaat ctgctctgat gccgcatagt 
     4501 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa 
     4561 tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta 
     4621 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga 
     4681 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc 
     4741 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat 
     4801 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc 
     4861 aatgggtgga ctatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc 
     4921 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt 
     4981 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta 
     5041 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg 
     5101 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac 
     5161 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg 
     5221 tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact 
     5281 ggcttatcga aattaatacg actcactata gggagaccca agctggctag cgtttaaact 
     5341 taagcttacc atggattaca aggacgatga cgataaggta ccaatggaga acgaggaaga 
     5401 ttcagatttc aatctgaaaa ttgaggaatc gaggtttgaa atagtgaagc ggttgaacct 
     5461 agatcgtact tcattattgg actatcttag gagcaaagga atttttgatg ctggtgactg 
     5521 tgatctggtt ttatcggaga aaactcgtga acacaaagct ggcaaattgt tggatatttt 
     5581 ggagacaaaa ggaaataatg ctgtcttgat ttttgtggat gcattacaat ttcagaatcc 
     5641 tgatttattc gagaagataa ctggaaaaaa agctaatcca ggtagcctgt ataacgacat 
     5701 gaccctcact ctagcggatt gtaggtatat tcctgatgtc gaaatcctaa gcggtcagtt 
     5761 gaagcagacg atcagcgatt tccacgacct tgccatgcgc tatcacgaag tgctcaaaga 
     5821 gaaccaatca cttggcaagc accttgacca ggcctccaca gacctggagg cgaaacacag 
     5881 gcgagtgggg atccttgaga agcagatgat cgacacggag gcgggcgtag cggaggcgca 
     5941 caagaacgcc agtcaactta gcgaggccgt tactgtacag ctaagaaacc tacagcaaat 
     6001 tatctgcgag aggaatacat ttattatcgc gttacaaatg aaattgctga cagcagaaga 
     6061 ggaagcgaag cagatgcgca aagttagcga gttcgagaag caatgcaggg agctccagtt 
     6121 tgtcaatcaa aagttgatcg ccgagcgaga cgagtctggg aaccaattga acgagctaaa 
     6181 ggactggacc gaggcgctca aagcaaaatt tgatcttgtc actgacgaat acaaacagct 
     6241 acagcaaagc ttcgtgaacg cggcggcaga caattgcaaa ctgaaggaga atgtggagga 
     6301 gttaaagctt cacttgtcgc taaataaaat tgcaacagcg gatctgaaaa actggaatga 
     6361 cgagttagag gagggtatca aagagtacag gaaacagcgg gatttctttt ctgagtcaag 
     6421 aaaagaagcc ttgcaagagc gtgacaatgc ccagaaggag cgtgacgagg ctgtacaaaa 
     6481 ataccatgac gtcatccaga cgtgcgacgc catggtcacg cgacagtgcg aacacactaa 
     6541 acactttgag gaaaaatacg acaaggcgct gaacgagctg aggccgctgc aaagaaagat 
     6601 cgcagccgca caacttgaga tcgaggagtt gaagagtaag ctgcgtgtgc atgacgacag 
     6661 agagaaggcg gagaggatga gctgcgaaca cctgaacgaa gttaatacca tcaaggatag 
     6721 aattgacgtc actcaactat acgagagcgt aagtgaggcg tcaagtccac agggttcccc 
     6781 tacaggaaaa ctggagggct tctcttgggc ggaccgtcgt gccacgacag ctataataga 
     6841 tagcgttcgc aagcgcgtgg cgggaacaca acaaacccaa gtcaaagtca tgagcctcga 
     6901 ccgcgcccta ataacaaaac ctgacgacac aaacccggat ctccttcaag caggcaaaat 
     6961 ggtcttcaaa tccgtccctt cctatggaac gctgcagtgc atgttcgggg ggtccttagg 
     7021 gagaaatgga ctcggaagcc ctgcggaagg gacaaccaca caagacactg gaaacgagca 
     7081 tagcctcgat tcgacgtcga caaattcgag tagaaactgc cacgaagaag gagcgagccc 
     7141 gcgtagtgaa ggctccaaaa gcagcggggc tgaaaacgat tgtaaagtgg aagaagacaa 
     7201 gccggaagta agtaaaccaa aaagtagacc tcatctgtat tcaaaaagcc tttccacctc 
     7261 ggcgttgctc ttaaacggga acaaggtgaa acatgctgtg aatgacgaac ttctagaaga 
     7321 agaatcgagc gatctatacg ataacgattc caccgatatc gacaactcgt ttcactgggt 
     7381 gcgaaccgac gagtgtggga accctttggt aggctcgcga tccgaggggg agcttcggcc 
     7441 cgagcaaggc gcgctaggaa tgacggaaaa tgaactacac gcattctata ataacctcat 
     7501 ccacaaaccg ctgaactttc gtcagagagc atgggcgatg aagaaaaagt cgactcgccg 
     7561 acagtcggag ccgacgattc gagtgctgca tgacaatgga acaaacctgg cgaagacaag 
     7621 ctgagggcc 
//
