LOCUS       pATE6Hy3                5748 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 1677.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|pATE6Hy3|
COMMENT     VNTOAUTHORNAME|BCCM/LMBP Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.lmbp@UGent.be|
COMMENT     VNTOAUTHORWWW|www.plasmids-lmbp.ugent.be/|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pATE6Hy3" 0 5748 
COMMENT       (CDBMol 0 0 1 1 1 0 0 1 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
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COMMENT        #8=(CFSignal (CObList) "INT" 15 0 0 1194 1336 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1194..1336") 
COMMENT        #9=(CFSignal (CObList) "H2" 21 0 0 1337 1381 0 (CStringList) 
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COMMENT        #10=(CFSignal (CObList) "INT" 15 0 0 1382 1524 0 (CStringList) 
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COMMENT        #11=(CFSignal (CObList) "H3" 21 0 0 1525 1569 0 (CStringList) 
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COMMENT        #18=(CFSignal (CObList) "3UTR" 21 0 0 2627 2743 0 (CStringList) 
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COMMENT        #19=(CFSignal (CObList) "ORI" 33 0 1 3735 4140 0 (CStringList) 
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COMMENT        #20=(CFSignal (CObList) "AMP" 4 0 1 4680 5540 0 (CStringList) 
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COMMENT                          "Foreign object. Author: UNKNOWN. Original author: BCCM/LMBP Plasmid Collection" 
COMMENT                          1) 
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COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                                        "antibiotic resistance gene; coding sequence" 
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FEATURES             Location/Qualifiers
     misc_feature    44..100
                     /vntifkey="21"
                     /label=L
                     /note="leader signal of E6H"
     CDS             101..460
                     /vntifkey="4"
                     /label=VH
                     /note="variable domain of E6H"
     misc_feature    461..751
                     /vntifkey="21"
                     /label=CH1
                     /note="chimeric constant domain 1 of the heavy chain"
     intron          752..1142
                     /vntifkey="15"
                     /label=INT
                     /note="intron"
     misc_feature    1143..1193
                     /vntifkey="21"
                     /label=H1
                     /note="exon of human ì3"
     intron          1194..1336
                     /vntifkey="15"
                     /label=INT
                     /note="intron"
     misc_feature    1337..1381
                     /vntifkey="21"
                     /label=H2
                     /note="exon of human ì3"
     intron          1382..1524
                     /vntifkey="15"
                     /label=INT
                     /note="intron"
     misc_feature    1525..1569
                     /vntifkey="21"
                     /label=H3
                     /note="exon of human ì3"
     intron          1570..1712
                     /vntifkey="15"
                     /label=INT
                     /note="intron"
     misc_feature    1713..1757
                     /vntifkey="21"
                     /label=H4
                     /note="exon of human ì3"
     intron          1758..1875
                     /vntifkey="15"
                     /label=INT
                     /note="intron"
     misc_feature    1876..2205
                     /vntifkey="21"
                     /label=CH2
                     /note="constant domain 2 of the human ì3 heavy chain"
     intron          2206..2302
                     /vntifkey="15"
                     /label=INT
                     /note="intron"
     misc_feature    2303..2626
                     /vntifkey="21"
                     /label=CH3
                     /note="constant domain 3 of the human ì3 heavy chain"
     misc_feature    2627..2743
                     /vntifkey="21"
                     /label=3UTR
                     /note="3' UTR of the human ì3 heavy chain"
     rep_origin      complement(3735..4140)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             complement(4680..5540)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
BASE COUNT     1299 a      1746 c      1479 g      1224 t 
ORIGIN
        1 ttctcatgtt tgacagctta tcatcgataa gcttggctct agcatggaat ggatctggat 
       61 ctttctcttc atcctgtcag gaactgcagg tgtccaatcc caggttcagc tgcagcagtc 
      121 tggagctgag ctggcgaggc ctggggcctc agtgaagctg tcctgcaagg cctctggata 
      181 caccctcaca agttatggta taagttgggt gaagcagaga actggacagg gccttgagtg 
      241 gattggagaa atttatcctg gaagtggaaa ttcgtacttc aatgagaagt ttaagggcaa 
      301 ggccacactg actgtagaca aatcctccag cacagcctac ttgcacctca gcagcctgac 
      361 atctgaggac tctgccgtct atttctgtgc cggacctaga caggtcgggc tactcccttt 
      421 tggttactgg ggccaaggga ctctggtcac tgcctctgca gccaaaacaa cacccccatc 
      481 agtctatcca ctggcccctg ggtgtggaga tacaactggt tcctccgtga ctctgggatg 
      541 cctggtcaag ggctacttcc ctgagtcagt gactgtgact tggaactccg gatccctgtc 
      601 cagcagtgtg cacaccttcc cagctctcct gcagtctgga ctctactccc tcagcagcgt 
      661 ggtgaccgtg ccctccagca gcttgggcac ccagacctac acctgcaacg tgaatcacaa 
      721 gcccagcaac accaaggtgg acaagagagt tggtgagagg ccagcgcagg gagggagggt 
      781 gtctgctgga agccaggctc agccctcctg cctggacgca tcccggctgt gcagtcccag 
      841 cccagggcag caaggcaggc cccgtctgac tcctcacccg gagcctctgc ccgccccact 
      901 catgctcagg gagagggtct tctggctttt tccaccaggc tccgggcagg cacaggctgg 
      961 atgcccctac cccaggccct tcacacacag gggcaggtgc tgcgctcaga gctgccaaaa 
     1021 gccatatcca ggaggaccct gcccctgacc taagcccacc ccaaaggcca aactctctac 
     1081 tcactcagct cagacacctt ctctcttccc agatctgagt aactcccaat cttctctctg 
     1141 cagagctcaa aaccccactt ggtgacacaa ctcacacatg cccacggtgc ccaggtaagc 
     1201 cagcccagga ctcgccctcc agctcaaggc gggacaagag ccctagagtg gcctgagtcc 
     1261 agggacaggc cccagcaggg tgctgacgca tccacctcca tcccagatcc ccgtaactcc 
     1321 caatcttctc tctgcagagc ccaaatcttg tgacacacct cccccgtgcc cacggtgccc 
     1381 aggtaagcca gcccaggcct caccctccag ctcaaggcag gacaagagcc ctagagtggc 
     1441 ctgagtccag ggacaggccc cagcagggtg ctgacgcgtc cacctccatc ccagatcccc 
     1501 gtaactccca atcttctctc tgcagagccc aaatcttgtg acacacctcc cccatgccca 
     1561 cggtgcccag gtaagccagc ccaggcctcg ccctccagct caaggcggga caagtaccct 
     1621 agagtggcct gagtccaggg acaggcccca gcagggtgct gacgcatcca cctccatccc 
     1681 agatccccgt aactcccaat cttctctctg cagagcccaa atcttgtgac acacctcccc 
     1741 cgtgcccaag gtgcccaggt aagccagccc aggcctcgcc ctccagctca aggcaggaca 
     1801 ggtgccctag agtggcctgc atccagggac aggtcccagt cgggtgctga cacatctgcc 
     1861 tccatctctt cctcagcacc tgaactcctg ggaggaccgt cagtcttcct cttcccccca 
     1921 aaacccaagg atacccttat gatttcccgg acccctgagg tcacgtgcgt ggtggtggac 
     1981 gtgagccacg aagaccccga ggtccagttc aagtggtacg tggacggcgt ggaggtgcat 
     2041 aatgccaaga caaagccgcg ggaggagcag tacaacagca cgttccgtgt ggtcagcgtc 
     2101 ctcaccgtcc tgcaccagga ctggctgaac ggcaaggagt acaagtgcaa ggtctccaac 
     2161 aaagccctcc cagcccccat cgagaaaacc atctccaaaa ccaaaggtgg gacccgcggg 
     2221 gtatgagggc cacatggaca gaggccagct tgacccaccc tctgccctgg gagtgaccgc 
     2281 tgtgccaacc tctgtcccta caggacagcc ccgagaacca caggtgtaca ccctgccccc 
     2341 atcccgggag gagatgacca agaaccaggt cagcctgacc tgcctggtca aaggcttcta 
     2401 ccccagcgac atcgccgtgg agtgggagag cagcgggcag ccggagaaca actacaacac 
     2461 cacgcctccc atgctggact ccgacggctc cttcttcctc tacagcaagc tcaccgtgga 
     2521 caagagcagg tggcagcagg ggaacatctt ctcatgctcc gtgatgcatg aggctctgca 
     2581 caaccgcttc acgcagaaga gcctctccct gtctccgggt aaatgagtgc gacagccggc 
     2641 aagcccccgc tccccgggct ctcggggtcg cgcgaggatg cttggcacgt accccgtgta 
     2701 catacttccc gggcacccag catggaaata aagcacccag gtcgaccgat gcccttgaga 
     2761 gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt 
     2821 atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt 
     2881 ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc 
     2941 ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc 
     3001 gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt cttgctggcg 
     3061 ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc 
     3121 gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag 
     3181 cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc gctgatcgtc 
     3241 acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc 
     3301 gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg ggccacctcg 
     3361 acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga attggagcca 
     3421 atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac atatccatcg 
     3481 cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg tcctggccac 
     3541 gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg ggttgcctta 
     3601 ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa 
     3661 cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg 
     3721 aaacgcggaa gtcagcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 
     3781 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 
     3841 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 
     3901 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 
     3961 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 
     4021 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 
     4081 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 
     4141 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 
     4201 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 
     4261 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 
     4321 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc 
     4381 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 
     4441 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 
     4501 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 
     4561 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 
     4621 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 
     4681 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 
     4741 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 
     4801 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 
     4861 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 
     4921 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 
     4981 gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 
     5041 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 
     5101 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 
     5161 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 
     5221 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 
     5281 tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 
     5341 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 
     5401 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 
     5461 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 
     5521 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 
     5581 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 
     5641 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca 
     5701 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaa 
//
