LOCUS       pATHIG32                5533 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 1676.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|pATHIG32|
COMMENT     VNTOAUTHORNAME|BCCM/LMBP Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.lmbp@UGent.be|
COMMENT     VNTOAUTHORWWW|www.plasmids-lmbp.ugent.be/|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pATHIG32" 0 5533 
COMMENT       (CDBMol 0 0 1 1 1 0 0 1 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
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COMMENT                          "Foreign object. Author: UNKNOWN. Original author: BCCM/LMBP Plasmid Collection" 
COMMENT                          1) 
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FEATURES             Location/Qualifiers
     misc_feature    30..242
                     /vntifkey="21"
                     /label=5FR
                     /note="5' flanking region of human immunoglobulin ì3 gene"
     misc_feature    243..536
                     /vntifkey="21"
                     /label=CH1
                     /note="constant domain 1 of the human ì3 heavy chain"
     intron          537..927
                     /vntifkey="15"
                     /label=INT
                     /note="intron of the human immunoglobulin ì3 gene"
     misc_feature    928..978
                     /vntifkey="21"
                     /label=H1
                     /note="exon 1 of HIG3"
     intron          979..1121
                     /vntifkey="15"
                     /label=INT
                     /note="intron of the human immunoglobulin ì3 gene"
     misc_feature    1122..1166
                     /vntifkey="21"
                     /label=H2
                     /note="exon 2 of HIG3"
     intron          1167..1309
                     /vntifkey="15"
                     /label=INT
                     /note="intron of the human immunoglobulin ì3 gene"
     misc_feature    1310..1368
                     /vntifkey="21"
                     /label=H3
                     /note="exon 3 of HIG3"
     intron          1369..1497
                     /vntifkey="15"
                     /label=INT
                     /note="intron of the human immunoglobulin ì3 gene"
     misc_feature    1498..1533
                     /vntifkey="21"
                     /label=H4
                     /note="exon 4 of HIG3"
     intron          1534..1660
                     /vntifkey="15"
                     /label=INT
                     /note="intron of the human immunoglobulin ì3 gene"
     misc_feature    1661..1990
                     /vntifkey="21"
                     /label=CH2
                     /note="constant domain 2 of the human ì3 heavy chain"
     intron          1991..2087
                     /vntifkey="15"
                     /label=INT
                     /note="intron of the human immunoglobulin ì3 gene"
     misc_feature    2088..2408
                     /vntifkey="21"
                     /label=CH3
                     /note="constant domain 3 of the human ì3 heavy chain"
     misc_feature    2409..2524
                     /vntifkey="21"
                     /label=3UTR
                     /note="3' UTR of the human immunoglobulin ì3 gene"
     rep_origin      complement(3520..3925)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             complement(4465..5325)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
BASE COUNT     1232 a      1721 c      1440 g      1140 t 
ORIGIN
        1 ttctcatgtt tgacagctta tcatcgataa gctttctggg gcaggccagg cctgaccttg 
       61 gctgggggca gggagggggc taaggtgacg caggtggcgc cagcaggcgc acacccaatg 
      121 cccgtgagcc cagacactgg accctgcctg gaccctcgtg gatagacaag aaccgagggg 
      181 cctctgcgcc ctgggcccag ctctgtccca caccgcagtc acatggcgcc atctctcttg 
      241 cagcttccac caagggccca tcggtcttcc ccctggcgcc ctgctccagg agcacctctg 
      301 ggggcacagc ggccctgggc tgcctggtca aggactactt ccccgaaccg gtgacggtgt 
      361 cgtggaactc aggcgccctg accagcggcg tgcacacctt cccggctgtc ctacagtcct 
      421 caggactcta ctccctcagc agcgtggtga ccgtgccctc cagcagcttg ggcacccaga 
      481 cctacacctg caacgtgaat cacaagccca gcaacaccaa ggtggacaag agagttggtg 
      541 agaggccagc gcagggaggg agggtgtctg ctggaagcca ggctcagccc tcctgcctgg 
      601 acgcatcccg gctgtgcagt cccagcccag ggcagcaagg caggccccgt ctgactcctc 
      661 acccggagcc tctgcccgcc ccactcatgc tcagggagag ggtcttctgg ctttttccac 
      721 caggctccgg gcaggcacag gctggatgcc cctaccccag gcccttcaca cacaggggca 
      781 ggtgctgcgc tcagagctgc caaaagccat atccaggagg accctgcccc tgacctaagc 
      841 ccaccccaaa ggccaaactc tctactcact cagctcagac accttctctc ttcccagatc 
      901 tgagtaactc ccaatcttct ctctgcagag ctcaaaaccc cacttggtga cacaactcac 
      961 acatgcccac ggtgcccagg taagccagcc caggactcgc cctccagctc aaggcgggac 
     1021 aagagcccta gagtggcctg agtccaggga caggccccag cagggtgctg acgcatccac 
     1081 ctccatccca gatccccgta actcccaatc ttctctctgc agagcccaaa tcttgtgaca 
     1141 cacctccccc gtgcccacgg tgcccaggta agccagccca ggcctcaccc tccagctcaa 
     1201 ggcaggacaa gagccctaga gtggcctgag tccagggaca ggccccagca gggtgctgac 
     1261 gcgtccacct ccatcccaga tccccgtaac tcccaatctt ctctctgcag agcccaaatc 
     1321 ttgtgacaca cctcccccat gcccacggtg cccaggtaag ccagcccagg cctcgccctc 
     1381 cagctcaagg cgggacaagt accctagagt ggcctgagtc cagggacagg ccccagcagg 
     1441 gtgctgacgc atccacctcc atcccagatc cccgtaactc ccaatcttct ctctgcagag 
     1501 cccaaatctt gtgacacacc tcccccgtgc ccaaggtgcc caggtaagcc agcccaggcc 
     1561 tcgccctcca gctcaaggca ggacaggtgc cctagagtgg cctgcatcca gggacaggtc 
     1621 ccagtcgggt gctgacacat ctgcctccat ctcttcctca gcacctgaac tcctgggagg 
     1681 accgtcagtc ttcctcttcc ccccaaaacc caaggatacc cttatgattt cccggacccc 
     1741 tgaggtcacg tgcgtggtgg tggacgtgag ccacgaagac cccgaggtcc agttcaagtg 
     1801 gtacgtggac ggcgtggagg tgcataatgc caagacaaag ccgcgggagg agcagtacaa 
     1861 cagcacgttc cgtgtggtca gcgtcctcac cgtcctgcac caggactggc tgaacggcaa 
     1921 ggagtacaag tgcaaggtct ccaacaaagc cctcccagcc cccatcgaga aaaccatctc 
     1981 caaaaccaaa ggtgggaccc gcggggtatg agggccacat ggacagaggc cagcttgacc 
     2041 caccctctgc cctgggagtg accgctgtgc caacctctgt ccctacagga cagccccgag 
     2101 aaccacaggt gtacaccctg cccccatccc gggaggagat gaccaagaac caggtcagcc 
     2161 tgacctgcct ggtcaaaggc ttctacccca gcgacatcgc cgtggagtgg gagagcagcg 
     2221 ggcagccgga gaacaactac aacaccacgc ctcccatgct ggactccgac ggctccttct 
     2281 tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac atcttctcat 
     2341 gctccgtgat gcatgaggct ctgcacaacc gcttcacgca gaagagcctc tccctgtctc 
     2401 cgggtaaatg agtgcgacag ccggcaagcc cccgctcccc gggctctcgg ggtcgcgcga 
     2461 ggatgcttgg cacgtacccc gtgtacatac ttcccgggca cccagcatgg aaataaagca 
     2521 cccaggtcga ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg 
     2581 gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca 
     2641 ggtgccggca gcgctctggg tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat 
     2701 gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac 
     2761 tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt atcgccggca tggcggccga 
     2821 cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct tccccattat 
     2881 gattcttctc gcttccggcg gcatcgggat gcccgcgttg caggccatgc tgtccaggca 
     2941 ggtagatgac gaccatcagg gacagcttca aggatcgctc gcggctctta ccagcctaac 
     3001 ttcgatcact ggaccgctga tcgtcacggc gatttatgcc gcctcggcga gcacatggaa 
     3061 cgggttggca tggattgtag gcgccgccct ataccttgtc tgcctccccg cgttgcgtcg 
     3121 cggtgcatgg agccgggcca cctcgacctg aatggaagcc ggcggcacct cgctaacgga 
     3181 ttcaccactc caagaattgg agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc 
     3241 aacccttggc agaacatatc catcgcgtcc gccatctcca gcagccgcac gcggcgcatc 
     3301 tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc 
     3361 cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat acgcgagcga 
     3421 acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg aatggtcttc 
     3481 ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag cgctcttccg cttcctcgct 
     3541 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 
     3601 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 
     3661 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 
     3721 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 
     3781 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 
     3841 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 
     3901 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 
     3961 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 
     4021 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 
     4081 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 
     4141 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 
     4201 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 
     4261 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 
     4321 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 
     4381 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 
     4441 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 
     4501 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 
     4561 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 
     4621 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 
     4681 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 
     4741 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg 
     4801 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 
     4861 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 
     4921 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 
     4981 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 
     5041 atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc 
     5101 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 
     5161 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 
     5221 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 
     5281 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 
     5341 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 
     5401 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 
     5461 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt 
     5521 tcgtcttcaa gaa 
//
