LOCUS       pATIPMC                 4904 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 1775.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|pATIPMC|
COMMENT     VNTOAUTHORNAME|BCCM/LMBP Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.lmbp@UGent.be|
COMMENT     VNTOAUTHORWWW|www.plasmids-lmbp.ugent.be/|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pATIPMC" 0 4904 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 4904 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (CObList) 
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COMMENT            1 1 1 1 "170..195") 
COMMENT        #4=(CFSignal (CObList) "INT" 15 0 0 196 883 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "196..883") 
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COMMENT            (CStringList) 1 1 1 1 "884..1149") 
COMMENT        #6=(CFSignal (CObList) "M1" 4 0 0 170 928 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "170..928") 
COMMENT        #7=(CFSignal (CObList) "TETs" 4 0 0 1332 2522 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1332..2522") 
COMMENT        #8=(CFSignal (CObList) "ORI" 33 0 1 2895 3295 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2895..3295)") 
COMMENT        #9=(CFSignal (CObList) "AMP" 4 0 1 3836 4696 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(3836..4696)")) (CObList) (CObList) 
COMMENT       (CObList) (CObList) (CObList) (CObList) 
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COMMENT                          "DNA 'pATIPMC'" 1) 
COMMENT                     #14=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Foreign object. Author: UNKNOWN. Original author: BCCM/LMBP Plasmid Collection" 
COMMENT                          1) 
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COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                          "Last Modification Date: ? (no data)" 1) 
COMMENT                     #17=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 4904 bp" 1) 
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COMMENT                          "storage type: Basic" 1) 
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COMMENT                                   #28=(CLinePar 
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COMMENT                                        "A/PR/8/34/MATRIX gene 1" 1) 
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COMMENT                                        "Start: 1332  End: 2522" 1) 
COMMENT                                   #33=(CLinePar 
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COMMENT                                        "antibiotic resistance gene, sensitive sequence" 
COMMENT                                        1) 
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COMMENT                                        "intron of A/PR/8/34/MATRIX gene 2" 1) 
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COMMENT                                        "2nd exon of A/PR/8/34 influenza matrix gene 2" 
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FEATURES             Location/Qualifiers
     misc_feature    170..195
                     /vntifkey="21"
                     /label=M2
                     /note="1st exon of A/PR/8/34 influenza matrix gene 2"
     intron          196..883
                     /vntifkey="15"
                     /label=INT
                     /note="intron of A/PR/8/34/MATRIX gene 2"
     misc_feature    884..1149
                     /vntifkey="21"
                     /label=M2
                     /note="2nd exon of A/PR/8/34 influenza matrix gene 2"
     CDS             170..928
                     /vntifkey="4"
                     /label=M1
                     /note="A/PR/8/34/MATRIX gene 1"
     CDS             1332..2522
                     /vntifkey="4"
                     /label=TETs
                     /note="antibiotic resistance gene, sensitive sequence"
     rep_origin      complement(2895..3295)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             complement(3836..4696)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
BASE COUNT     1176 a      1248 c      1252 g      1228 t 
ORIGIN
        1 ttctcatgtt tgacagctta tcatcgggaa gctagagtaa gtagttcgcc agttaatagt 
       61 ttgcgcaacg ttgttgccat tgctgcaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 
      121 aaaaaaaaaa aaaaaaaaaa aaaaagcgaa agcaggtaga tattgaaaga tgagtcttct 
      181 aaccgaggtc gaaacgtacg ttctctctat catcccgtca ggccccctca aagccgagat 
      241 cgcacagaga cttgaagatg tctttgcagg gaagaacacc gatcttgagg ttctcatgga 
      301 atggctaaag acaagaccaa tcctgtcacc tctgactaag gggattttag gatttgtgtt 
      361 cacgctcacc gtgcccagtg agcgaggact gcagcgtaga cgctttgtcc aaaatgccct 
      421 taatgggaac ggggatccaa ataacatgga caaagcagtt aaactgtata ggaagctcaa 
      481 gagggagata acattccatg gggccaaaga aatctcactc agttattctg ctggtgcact 
      541 tgccagttgt atgggcctca tatacaacag gatgggggct gtgaccactg aagtggcatt 
      601 tggcctggta tgtgcaacct gtgaacagat tgctgactcc cagcatcggt ctcataggca 
      661 aatggtgaca acaaccaacc cactaatcag acatgagaac agaatggttt tagccagcac 
      721 tacagctaag gctatggagc aaatggctgg atcgagtgag caagcagcag aggccatgga 
      781 ggttgctagt caggctaggc aaatggtgca agcgatgaga accattggga ctcatcctag 
      841 ctccagtgct ggtctgaaaa atgatcttct tgaaaatttg caggcctatc agaaacgaat 
      901 gggggtgcag atgcaacggt tcaagtgatc ctctcgctat tgccgcaaat atcattggga 
      961 tcttgcactt gatattgtgg attcttgatc gtcttttttt caaatgcatt taccgtcgct 
     1021 ttaaatacgg actgaaagga gggccttcta cggaaggagt gccaaagtct atgagggaag 
     1081 aatatcgaaa ggaacagcag agtgctgtgg atgctgacga tggtcatttt gtcagcatag 
     1141 agctggagtt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt 
     1201 tttttttttt tttttttttt gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 
     1261 cattcagctc cgataagctt taatgcggta gtttatcaca gttaaattgc taacgcagtc 
     1321 aggcaccgtg tatgaaatct aacaatgcgc tcatcgtcat cctcggcacc gtcaccctgg 
     1381 atgctgtagg cataggcttg gttatgccgg tactgccggg cctcttgcgg gatatcgtcc 
     1441 attccgacag catcgccagt cactatggcg tgctgctagc gctatatgcg ttgatgcaat 
     1501 ttctatgcgc acccgttctc ggagcactgt ccgaccgctt tggccgccgc ccagtcctgc 
     1561 tcgcttcgct acttggagcc actatcgact acgcgatcat ggcgaccaca cccgtcctgt 
     1621 ggatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg 
     1681 gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga 
     1741 gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca 
     1801 tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg 
     1861 gctgcttcct aatgcaggag tcgcataagg gagagcgtcg accgatgccc ttgagagcct 
     1921 tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga 
     1981 ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg 
     2041 gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa 
     2101 tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga 
     2161 agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg 
     2221 cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga 
     2281 tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc 
     2341 aaggatcgct cgcggctctt accagcctaa cttcgatcac tggaccgctg atcgtcacgg 
     2401 cgatttatgc cgcctcggcg agcacatgga acgggttggc atggattgta ggcgccgccc 
     2461 tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct 
     2521 gaatggaagc cggcggcacc tcgctaacgg attcaccact ccaagaattg gagccaatca 
     2581 attcttgcgg agaactgtga atgcgcaaac caacccttgg cagaacatat ccatcgcgtc 
     2641 cgccatctcc agcagccgca cgcggcgcat ctcgggcagc gttgggtcct ggccacgggt 
     2701 gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg 
     2761 ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc 
     2821 tgcgacctga gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac 
     2881 gcggaagtca gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 
     2941 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 
     3001 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 
     3061 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 
     3121 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 
     3181 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 
     3241 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 
     3301 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 
     3361 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 
     3421 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 
     3481 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 
     3541 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 
     3601 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 
     3661 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 
     3721 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 
     3781 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 
     3841 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 
     3901 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 
     3961 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 
     4021 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 
     4081 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 
     4141 ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 
     4201 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 
     4261 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 
     4321 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 
     4381 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 
     4441 gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 
     4501 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 
     4561 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 
     4621 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 
     4681 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 
     4741 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 
     4801 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa 
     4861 cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaa 
//
