LOCUS       pATMTNF3                4875 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 2528.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|pATMTNF3|
COMMENT     VNTOAUTHORNAME|BCCM/LMBP Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.lmbp@UGent.be|
COMMENT     VNTOAUTHORWWW|www.plasmids-lmbp.ugent.be/|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pATMTNF3" 0 4875 
COMMENT       (CDBMol 0 0 1 1 1 0 0 1 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 4875 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #3=(CFSignal (CObList) "TET" 4 0 0 86 1276 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "86..1276") 
COMMENT        #4=(CFSignal (CObList) "ORI" 33 0 1 1650 2050 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1650..2050)") 
COMMENT        #5=(CFSignal (CObList) "5UTR" 21 0 0 2939 3094 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "2939..3094") 
COMMENT        #6=(CFSignal (CObList) "pmTNF" 21 0 0 3095 3331 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "3095..3331") 
COMMENT        #7=(CFSignal (CObList) "mTNF" 4 0 0 3332 3802 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "3332..3802") 
COMMENT        #8=(CFSignal (CObList) "3UTR" 21 0 0 3803 4091 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "3803..4091")) (CObList) (CObList) (CObList) 
COMMENT       (CObList) (CObList) (CObList) 
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COMMENT                         "DNA 'pATMTNF3'" 1) 
COMMENT                    #13=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "Foreign object. Author: UNKNOWN. Original author: BCCM/LMBP Plasmid Collection" 
COMMENT                         1) 
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COMMENT                         "Creation Date: ? (no data)" 1) 
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COMMENT                         "Last Modification Date: ? (no data)" 1) 
COMMENT                    #16=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "length: 4875 bp" 1) 
COMMENT                    #17=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "storage type: Basic" 1) 
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COMMENT                         "form: Circular" 1))) "General Description") 
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COMMENT                         "Original Source Database: GenBank" 1))) 
COMMENT                  "Standard Fields") 
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COMMENT                                  #27=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "antibiotic resistance gene; coding sequence" 
COMMENT                                       1) 
COMMENT                                  #28=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Original Location Description:" 1) 
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COMMENT                                       "   86..1276" 1))) "TET") 
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COMMENT                                  #32=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "mouse tumor necrosis factor mature sequence" 
COMMENT                                       1) 
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COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #34=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "   3332..3802" 1))) "mTNF"))) 
COMMENT                         "CDS (2 total)") 
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COMMENT                                       "mouse tumor necrosis factor presequence" 
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COMMENT                                  #48=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "3' UTR of the mouse tumor necrosis factor gene" 
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FEATURES             Location/Qualifiers
     CDS             86..1276
                     /vntifkey="4"
                     /label=TET
                     /note="antibiotic resistance gene; coding sequence"
     rep_origin      complement(1650..2050)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     misc_feature    2939..3094
                     /vntifkey="21"
                     /label=5UTR
                     /note="5' UTR of the mouse tumor necrosis factor gene"
     misc_feature    3095..3331
                     /vntifkey="21"
                     /label=pmTNF
                     /note="mouse tumor necrosis factor presequence"
     CDS             3332..3802
                     /vntifkey="4"
                     /label=mTNF
                     /note="mouse tumor necrosis factor mature sequence"
     misc_feature    3803..4091
                     /vntifkey="21"
                     /label=3UTR
                     /note="3' UTR of the mouse tumor necrosis factor gene"
BASE COUNT     1093 a      1398 c      1261 g      1123 t 
ORIGIN
        1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 
       61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 
      121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 
      181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata 
      241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg 
      301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac 
      361 cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac 
      421 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca 
      481 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg 
      541 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct 
      601 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat 
      661 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt 
      721 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct 
      781 ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct 
      841 tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa 
      901 acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt 
      961 cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc 
     1021 cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca 
     1081 tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc 
     1141 gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat 
     1201 tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg 
     1261 ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga 
     1321 attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac 
     1381 atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg 
     1441 tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg 
     1501 ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc 
     1561 tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg 
     1621 taaagtctgg aaacgcggaa gtcagcgctc ttccgcttcc tcgctcactg actcgctgcg 
     1681 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 
     1741 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 
     1801 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 
     1861 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 
     1921 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 
     1981 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 
     2041 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 
     2101 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 
     2161 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 
     2221 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt 
     2281 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 
     2341 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 
     2401 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 
     2461 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 
     2521 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 
     2581 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 
     2641 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 
     2701 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 
     2761 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 
     2821 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 
     2881 tttgcgcaac gttgttgcca ttgctgcagg gggggggggg gggggggggg ggggggggcc 
     2941 tcagcgagga cagcaaggga ctagccagga gggagaacag aaactccaga acatcctgga 
     3001 aatagctccc agaaaagcaa gcagccaacc aggcaggttc tgtccctttc actcactggc 
     3061 ccaaggcgcc acatctccct ccagaaaaga caccatgagc acagaaagca tgatccgcga 
     3121 cgtggaactg gcagaagagg cactccccca aaagatgggg ggcttccaga actccaggcg 
     3181 gtgcctatgt ctcagcctct tctcattcct gcttgtggca ggggccacca cgctcttctg 
     3241 tctactgaac ttcggggtga tcggtcccca aagggatgag aagttcccaa atggcctccc 
     3301 tctcatcagt tctatggccc agaccctcac actcagatca tcttctcaaa attcgagtga 
     3361 caagcctgta gcccacgtcg tagcaaacca ccaagtggag gagcagctgg agtggctgag 
     3421 ccagcgcgcc aacgccctcc tggccaacgg catggatctc aaagacaacc aactagtggt 
     3481 gccagccgat gggttgtacc ttgtctactc ccaggttctc ttcaagggac aaggctgccc 
     3541 cgactacgtg ctcctcaccc acaccgtcag ccgatttgct atctcatacc aggagaaagt 
     3601 caacctcctc tctgccgtca agagcccctg ccccaaggac acccctgagg gggctgagct 
     3661 caaaccctgg tatgagccca tatacctggg aggagtcttc cagctggaga agggggacca 
     3721 actcagcgct gaggtcaatc tgcccaagta cttagacttt gcggagtccg ggcaggtcta 
     3781 ctttggagtc attgctctgt gaagggaatg ggtgttcatc cattctctac ccagccccca 
     3841 ctctgacccc tttactctga cccctttatt gtctactcct cagagccccc agtctgtgtc 
     3901 cttctaactt agaaagggga ttatggctca gagtccaact ctgtgctcag agctttcaac 
     3961 aactactcag aaacacaaga tgctgggaca gtgacctgga ctgtgggcct ctcatgcacc 
     4021 accatcaagg actcaaatgg gctttccgaa ttcactggag cctcgaatgt ccattcctga 
     4081 gttctgcaaa gccccccccc cccccccccc cccccccccc ctgcaggcat cgtggtgtca 
     4141 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 
     4201 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 
     4261 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 
     4321 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 
     4381 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg 
     4441 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 
     4501 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 
     4561 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 
     4621 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 
     4681 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 
     4741 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 
     4801 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc 
     4861 tttcgtcttc aagaa 
//
