LOCUS       pATMxpA2                4632 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 2694.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|pATMxpA2|
COMMENT     VNTOAUTHORNAME|BCCM/LMBP Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.lmbp@UGent.be|
COMMENT     VNTOAUTHORWWW|www.plasmids-lmbp.ugent.be/|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pATMxpA2" 0 4632 
COMMENT       (CDBMol 0 0 1 1 1 0 0 12 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 4632 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #3=(CFSignal (CObList) "Mx" 30 0 0 41 486 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "41..486") 
COMMENT        #4=(CFSignal (CObList) "(t)" 4 0 0 503 577 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "503..577") 
COMMENT        #5=(CFSignal (CObList) "INT" 15 0 0 578 644 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "578..644") 
COMMENT        #6=(CFSignal (CObList) "3UTR" 21 0 0 645 1196 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "645..1196") 
COMMENT        #7=(CFSignal (CObList) "polyA" 43 0 0 1197 1293 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1197..1293") 
COMMENT        #8=(CFSignal (CObList) "polyA" 43 0 1 1197 1293 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1197..1293)") 
COMMENT        #9=(CFSignal (CObList) "ORI" 33 0 1 2620 3025 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2620..3025)") 
COMMENT        #10=(CFSignal (CObList) "AMP" 4 0 1 3564 4424 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3564..4424)")) (CObList) 
COMMENT       (CObList) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CTextView 0 
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COMMENT              #12=(CRefLinePar 
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COMMENT                    (CObjectList 
COMMENT                     #14=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA 'pATMxpA2'" 1) 
COMMENT                     #15=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Foreign object. Author: UNKNOWN. Original author: BCCM/LMBP Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #16=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                          "Last Modification Date: ? (no data)" 1) 
COMMENT                     #18=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 4632 bp" 1) 
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COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "Original Source Database: GenBank" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                                        "Start: 503   End: 577 " 1) 
COMMENT                                   #29=(CLinePar 
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COMMENT                                        "part of the SV40 small t-antigen" 1) 
COMMENT                                   #30=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #31=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   503..577" 1))) "(t)") 
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COMMENT                                        "Start: 3564  End: 4424 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #34=(CLinePar 
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COMMENT                                        "antibiotic resistance gene; coding sequence" 
COMMENT                                        1) 
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COMMENT                          "CDS (2 total)") 
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COMMENT                                        "3' UTR of the SV40 small t-antigen" 1) 
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COMMENT                                        "Original Location Description:" 1) 
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COMMENT                          "Misc. Feature (1 total)") 
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COMMENT                                   #52=(CLinePar 
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COMMENT                                        "mouse Mx promoter" 1) 
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COMMENT                          "Promoter Prokaryotic (1 total)") 
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COMMENT                                        "Start: 2620  End: 3025 (Complementary)" 
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COMMENT                                        "origin plasmid" 1) 
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COMMENT                          "Replication Origin (1 total)") 
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COMMENT                          "Terminator (2 total)"))) "Feature Map")))) 
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FEATURES             Location/Qualifiers
     promoter        41..486
                     /vntifkey="30"
                     /label=Mx
                     /note="mouse Mx promoter"
     CDS             503..577
                     /vntifkey="4"
                     /label=(t)
                     /note="part of the SV40 small t-antigen"
     intron          578..644
                     /vntifkey="15"
                     /label=INT
                     /note="intron of the SV40 small t-antigen"
     misc_feature    645..1196
                     /vntifkey="21"
                     /label=3UTR
                     /note="3' UTR of the SV40 small t-antigen"
     terminator      1197..1293
                     /vntifkey="43"
                     /label=polyA
                     /note="polyA signal of the SV40 early region"
     terminator      complement(1197..1293)
                     /vntifkey="43"
                     /label=polyA
                     /note="polyA signal of the SV40 late region"
     rep_origin      complement(2620..3025)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             complement(3564..4424)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
BASE COUNT     1129 a      1159 c      1154 g      1190 t 
ORIGIN
        1 ttctcatgtt tgacagctta tcatcgataa gcttgggctg cagtagcagt gctcagacct 
       61 ctaaacctgt agtcacaagg gacccagaag ggctggcagg gctcctcagt gattctggta 
      121 tggtttgtct ctgacccttc ctagtgcagc tggaagaatc cagtgaaaag agaaagttag 
      181 tctaggttgt gttcaactga gaggaggtca ggctgaaggc tcagcacaga gctggaaggg 
      241 tcctgggaaa gcaggcagcc atctgaactg gtgtccatac tgcatgtgtc ttggtgggct 
      301 gagcctacaa tatcgatttc ccaacctcag taccaagcca aggcccctca gggtgagttt 
      361 cgtttctgag ctcctttcat atgtggtggg ggtgtgggca cagcaagctc aggctttttc 
      421 acggtttcaa ttctcctctg gaaggggcgg agccagcact atttaaagag gtctgagccc 
      481 tggcaggact ctagagtcga cggatctttg tgaaggaacc ttacttctgt ggtgtgacat 
      541 aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg 
      601 tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa 
      661 ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag 
      721 aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa 
      781 agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc 
      841 atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag 
      901 ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata 
      961 acagttataa tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta 
     1021 ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata 
     1081 aggaatattt gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta 
     1141 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg 
     1201 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat 
     1261 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 
     1321 aaactcatca atgtatctta tcatgtctgg atcctctacg ccggacgcat cgtggccggc 
     1381 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa 
     1441 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc 
     1501 cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg 
     1561 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga 
     1621 gagcgtcgac cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg 
     1681 ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag 
     1741 gtgccggcag cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg 
     1801 atcggcctgt cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact 
     1861 ggtcccgcca ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggccgac 
     1921 gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt ccccattatg 
     1981 attcttctcg cttccggcgg catcgggatg cccgcgttgc aggccatgct gtccaggcag 
     2041 gtagatgacg accatcaggg acagcttcaa ggatcgctcg cggctcttac cagcctaact 
     2101 tcgatcactg gaccgctgat cgtcacggcg atttatgccg cctcggcgag cacatggaac 
     2161 gggttggcat ggattgtagg cgccgcccta taccttgtct gcctccccgc gttgcgtcgc 
     2221 ggtgcatgga gccgggccac ctcgacctga atggaagccg gcggcacctc gctaacggat 
     2281 tcaccactcc aagaattgga gccaatcaat tcttgcggag aactgtgaat gcgcaaacca 
     2341 acccttggca gaacatatcc atcgcgtccg ccatctccag cagccgcacg cggcgcatct 
     2401 cgggcagcgt tgggtcctgg ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc 
     2461 ggctaggctg gcggggttgc cttactggtt agcagaatga atcaccgata cgcgagcgaa 
     2521 cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc aacaacatga atggtcttcg 
     2581 gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc gctcttccgc ttcctcgctc 
     2641 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 
     2701 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 
     2761 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 
     2821 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 
     2881 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 
     2941 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 
     3001 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 
     3061 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 
     3121 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 
     3181 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 
     3241 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 
     3301 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 
     3361 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 
     3421 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 
     3481 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 
     3541 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 
     3601 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 
     3661 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 
     3721 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 
     3781 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 
     3841 tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc 
     3901 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 
     3961 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 
     4021 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 
     4081 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 
     4141 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca 
     4201 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 
     4261 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 
     4321 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 
     4381 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 
     4441 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 
     4501 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc 
     4561 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt 
     4621 cgtcttcaag aa 
//
