LOCUS       pBRHIG1                 5100 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 1672.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|pBRHIG1|
COMMENT     VNTOAUTHORNAME|BCCM/LMBP Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.lmbp@UGent.be|
COMMENT     VNTOAUTHORWWW|www.plasmids-lmbp.ugent.be/|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pBRHIG1" 0 5100 
COMMENT       (CDBMol 0 0 1 1 1 0 0 1114206 0 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
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COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT        #8=(CFSignal (CObList) "CH2" 21 0 0 1083 1412 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1083..1412") 
COMMENT        #9=(CFSignal (CObList) "INT" 15 0 0 1413 1508 0 (CStringList) 
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COMMENT        #10=(CFSignal (CObList) "CH3" 21 0 0 1509 1832 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1509..1832") 
COMMENT        #11=(CFSignal (CObList) "3UTR" 21 0 0 1833 1963 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1833..1963") 
COMMENT        #12=(CFSignal (CObList) "3FR" 21 0 0 1964 2802 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1964..2802") 
COMMENT        #13=(CFSignal (CObList) "ORI" 33 0 1 3090 3460 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3090..3460)") 
COMMENT        #14=(CFSignal (CObList) "AMP" 4 0 1 4032 4892 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4032..4892)")) (CObList) 
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COMMENT                          "DNA 'pBRHIG1'" 1) 
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COMMENT                          "Foreign object. Author: UNKNOWN. Original author: BCCM/LMBP Plasmid Collection" 
COMMENT                          1) 
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COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                          "Last Modification Date: ? (no data)" 1) 
COMMENT                     #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5100 bp" 1) 
COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "Original Source Database: GenBank" 1))) 
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COMMENT                                        "Start: 4032  End: 4892 (Complementary)" 
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COMMENT                                   #33=(CLinePar 
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COMMENT                                        "antibiotic resistance gene; coding sequence" 
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COMMENT                                        "intron of the human immunoglobulin \3541 gene" 
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COMMENT                                        "intron of the human immunoglobulin \3541 gene" 
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COMMENT                                        "intron of the human immunoglobulin \3541 gene" 
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FEATURES             Location/Qualifiers
     misc_feature    30..237
                     /vntifkey="21"
                     /label=5FR
                     /note="5' flanking region of human immunoglobulin ì1 gene"
     misc_feature    238..531
                     /vntifkey="21"
                     /label=CH1
                     /note="constant domain 1 of the human ì1 heavy chain"
     intron          532..919
                     /vntifkey="15"
                     /label=INT
                     /note="intron of the human immunoglobulin ì1 gene"
     misc_feature    920..964
                     /vntifkey="21"
                     /label=HINGE
                     /note="hinge domain of the human ì1 heavy chain"
     intron          965..1082
                     /vntifkey="15"
                     /label=INT
                     /note="intron of the human immunoglobulin ì1 gene"
     misc_feature    1083..1412
                     /vntifkey="21"
                     /label=CH2
                     /note="constant domain 2 of the human ì1 heavy chain"
     intron          1413..1508
                     /vntifkey="15"
                     /label=INT
                     /note="intron of the human immunoglobulin ì1 gene"
     misc_feature    1509..1832
                     /vntifkey="21"
                     /label=CH3
                     /note="constant domain 3 of the human ì1 heavy chain"
     misc_feature    1833..1963
                     /vntifkey="21"
                     /label=3UTR
                     /note="3' UTR of the human immunoglobulin ì1 gene"
     misc_feature    1964..2802
                     /vntifkey="21"
                     /label=3FR
                     /note="3' flanking region of human immunoglobulin ì1 gene"
     rep_origin      complement(3090..3460)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             complement(4032..4892)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
BASE COUNT     1008 a      1285 c      1139 g       904 t       764 others
ORIGIN
        1 ttctcatgtt tgacagctta tcatcgataa gctttctggg gcaggccagg cctgaccttg 
       61 gctttggggc agggaggggg ctaaggtgag gcaggtggcg ccagcaggtg cacacccaat 
      121 gcccatgagc ccagacactg gacgctgaac ctcgcggaca gttaagaacc caggggcctc 
      181 tgcgcctggg cccagctctg tcccacaccg cggtcacatg gcaccacctc tcttgcagcc 
      241 tccaccaagg gcccatcggt cttccccctg gcaccctcct ccaagagcac ctctgggggc 
      301 acagcggccc tgggctgcct ggtcaaggac tacttccccg aaccggtgac ggtgtcgtgg 
      361 aactcaggcg ccctgaccag cggcgtgcac accttcccgg ctgtcctaca gtcctcagga 
      421 ctctactccc tcagcagcgt ggtgaccgtg ccctccagca gcttgggcac ccagacctac 
      481 atctgcaacg tgaatcacaa gcccagcaac accaaggtgg acaagaaagt tggtgagagg 
      541 ccagcacagg gagggagggt gtctgctgga agcaggctca gcgctcctgc ctggacgcat 
      601 cccggctatg cagccccagt ccagggcagc aaggcaggcc ccgtctgcct cttcacccgg 
      661 agcctctgcc cgccccactc atgctcaggg agagggtctt ctggcttttt cccaggctct 
      721 gggcaggcac aggctaggtg cccctaaccc aggccctgca cacaaagggg caggtgctgg 
      781 gctcagacct gccaagagcc atatccggga ggaccctgcc cctgacctaa gcccacccca 
      841 aaggccaaac tctccactcc ctcagctcgg acaccttctc tcctcccaga ttccagtaac 
      901 tcccaatctt ctctctgcag agcccaaatc ttgtgacaaa actcacacat gcccaccgtg 
      961 cccaggtaag ccagcccagg cctcgccctc cagctcaagg cgggacaggt gccctagagt 
     1021 agcctgcatc cagggacagg ccccagccgg gtgctgacac gtccacctcc atctcttcct 
     1081 cagcacctga actcctgggg ggaccgtcag tcttcctctt ccccccaaaa cccaaggaca 
     1141 ccctcatgat ctcccggacc cctgaggtca catgcgtggt ggtggacgtg agccacgaag 
     1201 accctgaggt caagttcaac tggtacgtgg acggcgtgga ggtgcataat gccaagacaa 
     1261 agccgcggga ggagcagtac aacagcacgt accgggtggt cagcgtcctc accgtcctgc 
     1321 accaggactg gctgaatggc aaggagtaca agtgcaaggt ctccaacaaa gccctcccag 
     1381 cccccatcga gaaaaccatc tccaaagcca aaggtgggac ccgtggggtg cgagggccac 
     1441 atggacagag gccggctcgg cccaccctct gccctgagag tgaccgctgt accaacctct 
     1501 gtcctacagg gcagccccga gaaccacagg tgtacaccct gcccccatcc cgggatgagc 
     1561 tgaccaagaa ccaggtcagc ctgacctgcc tggtcaaagg cttctatccc agcgacatcg 
     1621 ccgtggagtg ggagagcaat gggcagccgg agaacaacta caagaccacg cctcccgtgc 
     1681 tggactccga cggctccttc ttcctctaca gcaagctcac cgtggacaag agcaggtggc 
     1741 agcaggggaa cgtcttctca tgctccgtga tgcatgaggc tctgcacaac cactacacgc 
     1801 agaagagcct ctccctgtct ccgggtaaat gagtgcgacg gccggcaagc cccgctcccc 
     1861 gggctctcgc ggtcgcacga ggatgcttgg cacgtacccc ctgtacatac ttcccgggcg 
     1921 cccagcatgg aaataaagca cccagcgctg ccctgggccc ctgcgagact gtgatggttc 
     1981 tttccacggg tcaggccgag tctgaggcct gagtggcatg agggaggcag agcgggtcnn 
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncagctgcc tcgcgcgttt 
     2821 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct 
     2881 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg 
     2941 tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat 
     3001 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga 
     3061 tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg 
     3121 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 
     3181 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 
     3241 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 
     3301 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 
     3361 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 
     3421 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 
     3481 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 
     3541 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 
     3601 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 
     3661 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta 
     3721 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 
     3781 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 
     3841 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 
     3901 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 
     3961 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 
     4021 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 
     4081 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 
     4141 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 
     4201 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 
     4261 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 
     4321 agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt 
     4381 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 
     4441 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 
     4501 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 
     4561 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 
     4621 cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact 
     4681 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 
     4741 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 
     4801 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 
     4861 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 
     4921 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 
     4981 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt 
     5041 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa 
//
