LOCUS       pCD-F-EIF4B             7224 bp    DNA     circular      1-APR-2009
DEFINITION  LMBP 6359.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|514392214|
COMMENT     VNTNAME|pCD-F-EIF4B|
COMMENT     VNTOAUTHORNAME|BCCM/LMBP Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.lmbp@UGent.be|
COMMENT     VNTOAUTHORWWW|www.plasmids-lmbp.ugent.be/|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCD-F-EIF4B" 0 7224 
COMMENT       (CDBMol 0 0 1 4469 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 1 0 10 10 40 50 0 1 0 
COMMENT        (CHomObj 0 0 0 3 75) (CWordArray 84 27) (CWordArray) 
COMMENT        (CStringList "ApaI" "KpnI") (CStringList "atg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 7224 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "ApaI" "gggccc" 6 0 1 2794 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 951 0 0 "")) 
COMMENT       (CObList 
COMMENT        #2=(CFSignal (CObList) "pCMV" 29 0 0 209 863 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "209..863") 
COMMENT        #3=(CFSignal (CObList) "T7" 30 0 0 863 882 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "863..882") 
COMMENT        #4=(CFSignal (CObList) "BGH polyA" 25 0 0 2813 3027 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "2804..3018") 
COMMENT        #5=(CFSignal (CObList) "f1" 33 0 0 3090 3503 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "3081..3494") 
COMMENT        #6=(CFSignal (CObList) "SV40" 29 0 0 3568 3893 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "3559..3884") 
COMMENT        #7=(CFSignal (CObList) "NeoR" 86 0 0 3929 4723 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "3920..4714") 
COMMENT        #8=(CFSignal (CObList) "SV40 polyA" 25 0 0 4739 4978 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "4730..4969") 
COMMENT        #9=(CFSignal (CObList) "ColE1" 33 0 0 5410 6083 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "5401..6074") 
COMMENT        #10=(CFSignal (CObList) "AmpR" 22 0 1 6228 7089 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(6219..7080)") 
COMMENT        #11=(CFSignal (CObList) "flag epitope" 21 0 0 920 946 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "920..946") 
COMMENT        #12=(CFSignal (CObList) "1503" 27 0 1 2811 2827 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2802..2818)") 
COMMENT        #13=(CFSignal (CObList) "2506" 27 0 0 863 882 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "863..882") 
COMMENT        #14=(CFSignal (CObList) "EIF4B" 4 0 0 953 2788 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #15=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #16=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "pCD-F-EIF4B" 2) 5 
COMMENT                   "" 0 4) 
COMMENT              #17=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #18=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA 'pCD-F-EIF4B'" 1) 
COMMENT                     #19=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: Peter Marynen" 
COMMENT                          1) 
COMMENT                     #20=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 04/01/09 02:43" 1) 
COMMENT                     #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 04/01/09 02:43" 1) 
COMMENT                     #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 7224 bp" 1) 
COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #25=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 3-JUL-2006" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #28=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 5 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Peter Marynen" 1))) "Original Author") 
COMMENT              #30=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #31=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) 
COMMENT                   "Annotations") 
COMMENT              #32=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #33=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #34=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 953 (3 #14# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #35=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 953   End: 2788" 1))) "EIF4B"))) 
COMMENT                          "CDS (1 total)") 
COMMENT                     #36=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #37=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 920 (3 #11# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #38=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 920   End: 946 " 1) 
COMMENT                                   #39=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #40=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   920..946" 1))) "flag epitope"))) 
COMMENT                          "Misc. Feature (1 total)") 
COMMENT                     #41=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 22 (7 22 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #42=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 6228 (3 #10# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #43=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 6228  End: 7089 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #44=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #45=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(6219..7080)" 1))) "AmpR"))) 
COMMENT                          "Misc. Marker (1 total)") 
COMMENT                     #46=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 25 (7 25 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #47=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2813 (3 #4# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #48=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2813  End: 3027" 1) 
COMMENT                                   #49=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #50=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2804..3018" 1))) "BGH polyA") 
COMMENT                            #51=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4739 (3 #8# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #52=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4739  End: 4978" 1) 
COMMENT                                   #53=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #54=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4730..4969" 1))) "SV40 polyA"))) 
COMMENT                          "PolyA Signal (2 total)") 
COMMENT                     #55=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 27 (7 27 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #56=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 863 (3 #13# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #57=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 863   End: 882 " 1) 
COMMENT                                   #58=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #59=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   863..882" 1))) "2506") 
COMMENT                            #60=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2811 (3 #12# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2811  End: 2827 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #62=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #63=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(2802..2818)" 1))) "1503"))) 
COMMENT                          "Primer (2 total)") 
COMMENT                     #64=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #65=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 209 (3 #2# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 209   End: 863 " 1) 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   209..863" 1))) "pCMV") 
COMMENT                            #69=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3568 (3 #6# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #70=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3568  End: 3893" 1) 
COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3559..3884" 1))) "SV40"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
COMMENT                     #73=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #74=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 863 (3 #3# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 863   End: 882 " 1) 
COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   863..882" 1))) "T7"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
COMMENT                     #78=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #79=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3090 (3 #5# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #80=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3090  End: 3503" 1) 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3081..3494" 1))) "f1") 
COMMENT                            #83=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5410 (3 #9# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5410  End: 6083" 1) 
COMMENT                                   #85=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   5401..6074" 1))) "ColE1"))) 
COMMENT                          "Replication Origin (2 total)") 
COMMENT                     #87=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 86 (7 86 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #88=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3929 (3 #7# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #89=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3929  End: 4723" 1) 
COMMENT                                   #90=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3920..4714" 1))) "NeoR"))) 
COMMENT                          "Misc. Recombination (1 total)"))) "Feature Map") 
COMMENT              #92=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #93=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 17205408 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #94=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #0# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #95=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #0# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 2794 " 1))))) "ApaI: 1 site") 5 1 
COMMENT                          "GGGCCC" "CCCGGG") 
COMMENT                     #96=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 17204776 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #97=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #1# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #98=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #1# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 951 " 1))))) "KpnI: 1 site") 5 1 
COMMENT                          "GGTACC" "CCATGG"))) "Restriction/Methylation Map")))) 
COMMENT       (CGraphView 
COMMENT        (CWStyleSheet 
COMMENT         (CObjectList 
COMMENT          #99=(CWidgetStyle "Annotation" 0 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 0 
COMMENT               0) 
COMMENT          #100=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 128 1 5 "@N (@S)" 0) 
COMMENT          #101=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 
COMMENT                1 2 "?" 0) 
COMMENT          #102=(CWidgetStyle "Mark 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 
COMMENT                0) 
COMMENT          #103=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 16744512) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 8388608 
COMMENT                1 65535 "@N (@H)" 0) 
COMMENT          #104=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 8437824) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 5 "@N (@S)" 0) 
COMMENT          #105=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #106=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT                "@F bp (molecule @L bp)" 0) 
COMMENT          #107=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 
COMMENT                "Fragment of @N" 0) 
COMMENT          #108=(CWidgetStyle "Molecule Label 2" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 0.888889 0 
COMMENT                1 16 "@L bp" 0) 
COMMENT          #109=(CWidgetStyle "Molecule Label 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.833333 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #110=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 2 8388608 5) 0 0 0) 
COMMENT          #111=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 26367 0) 
COMMENT                0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #112=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 
COMMENT                0 0) 
COMMENT          #113=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16728256 0) 0 0 1 (LOGSHAPE 8 1 0 0 0)) 
COMMENT          #114=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 
COMMENT                0) 
COMMENT          #115=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 0) 2 1 (LOGBRUSH 0 12632256 0) 
COMMENT                0 0 0) 
COMMENT          #116=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 16384) 8 0 0 0 1 
COMMENT                (LOGSHAPE 7 0.2 3.41182 2.86186 0.609808)) 
COMMENT          #117=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) 
COMMENT          #118=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #119=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #120=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) 
COMMENT          #121=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) 
COMMENT          #122=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #123=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #124=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 8388608) 10 0 0 0 0) 
COMMENT          #125=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #126=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #127=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                1 3 "@T @N " 0) 
COMMENT          #128=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #129=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #130=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #131=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #132=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 49216) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 65535 "@N" 0) 
COMMENT          #133=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 
COMMENT                "@N" 0) 
COMMENT          #134=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 3381504 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 
COMMENT                (LOGSHAPE 8 1 0 0 0)) 
COMMENT          #135=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 
COMMENT                1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #136=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 
COMMENT                1 1 "@N" 0) 
COMMENT          #137=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #138=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 
COMMENT                "@N (@S)" 0) 
COMMENT          #139=(CWidgetStyle "Shape 6" 1 (LOGPEN 0 0 16724787) 1 1 
COMMENT                (LOGBRUSH 0 128 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #140=(CWidgetStyle "Shape 7" 1 (LOGPEN 0 0 16724787) 1 1 
COMMENT                (LOGBRUSH 0 13382451 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #141=(CWidgetStyle "Shape 8" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 13382451 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #142=(CWidgetStyle "Shape 9" 1 (LOGPEN 0 0 32768) 8 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #143=(CWidgetStyle "Shape 10" 1 (LOGPEN 0 0 32768) 8 1 
COMMENT                (LOGBRUSH 2 16777215 1) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #144=(CWidgetStyle "Shape 11" 1 (LOGPEN 0 0 32768) 8 1 
COMMENT                (LOGBRUSH 2 0 1) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #145=(CWidgetStyle "Shape 12" 1 (LOGPEN 0 0 6684927) 8 1 
COMMENT                (LOGBRUSH 2 0 1) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #146=(CWidgetStyle "Shape 13" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 6750207 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 
COMMENT                (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #147=(CWidgetStyle "Shape 14" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 3368448 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 
COMMENT                (LOGSHAPE 8 1 0 0 0)) 
COMMENT          #148=(CWidgetStyle "Label 0" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 1.27778 0 1 65535 
COMMENT                "@N" 0) 
COMMENT          #149=(CWidgetStyle "Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 0 1 
COMMENT                65535 "@N" 0) 
COMMENT          #150=(CWidgetStyle "Shape 15" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #151=(CWidgetStyle "Shape 16" 1 (LOGPEN 0 0 0) 3 1 
COMMENT                (LOGBRUSH 0 26367 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 
COMMENT                (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #152=(CWidgetStyle "Shape 17" 1 (LOGPEN 0 0 153) 1 1 
COMMENT                (LOGBRUSH 0 16728256 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 
COMMENT                (LOGSHAPE 8 1 0 0 0)) 
COMMENT          #153=(CWidgetStyle "Shape 18" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 10040217 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 
COMMENT                (LOGSHAPE 8 1 0 0 0)) 
COMMENT          #154=(CWidgetStyle "Shape 19" 1 (LOGPEN 5 0 16711680) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0)) 
COMMENT         0.109543 1.16039 0.164644 2.53336 
COMMENT         (14 (CShapeMapEntry 8 "Shape 13" 1 "Signal Label") 12 
COMMENT          (CShapeMapEntry 8 "Line 2" 1 "Signal Label") 31 
COMMENT          (CShapeMapEntry 0 "Shape 15" 1 "Signal Label") 29 
COMMENT          (CShapeMapEntry 0 "Shape 9" 1 "Signal Label") 28 
COMMENT          (CShapeMapEntry 9 "Shape 2" 1 "Signal Label") 27 
COMMENT          (CShapeMapEntry 0 "Shape 19" 0 "Signal Label") 26 
COMMENT          (CShapeMapEntry 8 "Shape 4" 1 "Signal Label") 23 
COMMENT          (CShapeMapEntry 0 "Line 0" 0 "Signal Label") 22 
COMMENT          (CShapeMapEntry 0 "Shape 8" 1 "Signal Label") 21 
COMMENT          (CShapeMapEntry 0 "Shape 5" 1 "Signal Label") 37 
COMMENT          (CShapeMapEntry 8 "Shape 13" 1 "Signal Label") 34 
COMMENT          (CShapeMapEntry 8 "Shape 2" 1 "Signal Label") 33 
COMMENT          (CShapeMapEntry 8 "Shape 12" 1 "Signal Label") 59 
COMMENT          (CShapeMapEntry 0 "Shape 2" 1 "Signal Label") 62 
COMMENT          (CShapeMapEntry 0 "Shape 1" 0 "Signal Label") 61 
COMMENT          (CShapeMapEntry 8 "Shape 3" 1 "Signal Label") 70 
COMMENT          (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 
COMMENT          (CShapeMapEntry 0 "ORF" 0 "ORF Label") 94 
COMMENT          (CShapeMapEntry 5 "Shape 0" 1 "Signal Label") 87 
COMMENT          (CShapeMapEntry 0 "Shape 4" 1 "Signal Label") 86 
COMMENT          (CShapeMapEntry 0 "Shape 14" 1 "Signal Label") 85 
COMMENT          (CShapeMapEntry 9 "Shape 1" 1 "Signal Label") 84 
COMMENT          (CShapeMapEntry 9 "Shape 18" 1 "Signal Label") 96 
COMMENT          (CShapeMapEntry 7 "Shape 3" 1 "Signal Label") 99 
COMMENT          (CShapeMapEntry 9 "Line 0" 1 "Signal Label")) 42.2507 42.2507 39 39 
COMMENT         0.1 -5957) 1 1 0 1 1 
COMMENT        (mapper: 15.7136 -12.4711 42.2507 42.2507 0.01 10 -1 7224 7224 1 0 0) 
COMMENT        #155=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil 24822156 100) 
COMMENT              (CObjectList 
COMMENT               #156=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil 431 100) 
COMMENT                     (CObjectList 
COMMENT                      #157=(CAxis 
COMMENT                            (CWideLine (CWidget 0 (0 0) 1 2 0 0 #115# 599 0) 
COMMENT                             (LOGPEN 0 0 0) 2 (LOGBRUSH 0 12632256 0) 1 6.27505 
COMMENT                             6.27305 1 0.0142406) 0.0868381) 
COMMENT                      #158=(CLabel (CWidget 1001 (0 0) 1 2 0 0 #109# 734 100) 
COMMENT                            (LOGPEN 696 -102 4294967197) 650 
COMMENT                            (LOGFONT 40 15 0 0 700 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                            1.16039 0.833333 0 "pCD-F-EIF4B" "@N" 1 0 0.580195 0 
COMMENT                            -10 4.5443 0.899393 Nil) 
COMMENT                      #159=(CLabel (CWidget 1002 (0 0) 1 2 0 0 #108# 995 100) 
COMMENT                            (LOGPEN 973 -787 4294966481) 921 
COMMENT                            (LOGFONT 43 16 0 0 400 0 0 0 0 3 2 1 49 
COMMENT                             "Courier New") 1.16039 0.888889 0 "7224 bp" "@L bp" 
COMMENT                            16 0 -0.580195 0 -10.8 2.67451 1.0414 Nil)) 
COMMENT                     (CObjectList)) 
COMMENT               #160=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil 226 100) 
COMMENT                     (CObjectList 
COMMENT                      #161=(CGroupWidget 
COMMENT                            (CWidget 4 (7 4 0) 1 2 0 0 Nil 1743006086 100) 
COMMENT                            (CObjectList 
COMMENT                             #162=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #14# 0) 1 2 0 0 #111# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 26367 0) 1 
COMMENT                                    3.85214 5.44649 1 0.0547715) 0.8 1.8 0) 
COMMENT                             #163=(CLabel (CWidget 0 (0 0) 1 2 0 0 #105# 0 100) 
COMMENT                                   (LOGPEN 0 0 4227264) 1 
COMMENT                                   (LOGFONT 29 10 0 0 400 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.16039 0.611111 0 "EIF4B" "@N" 1 
COMMENT                                   13.1046 -1.16039 0 -10 1.53844 0.733716 #162#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #164=(CGroupWidget 
COMMENT                            (CWidget 21 (7 21 0) 1 2 0 0 Nil 16384248 100) 
COMMENT                            (CObjectList 
COMMENT                             #165=(CScratch 
COMMENT                                   (CWidget 0 (3 #11# 0) 1 2 0 0 #124# 8 100) 
COMMENT                                   (LOGPEN 0 8 8388608) 10 1 5.47514 1.9 
COMMENT                                   0.0547715 1) 
COMMENT                             #166=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #105# 17109928 100) 
COMMENT                                   (LOGPEN 0 0 4227264) 1 
COMMENT                                   (LOGFONT 29 10 0 0 400 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.16039 0.611111 0 "flag epitope" 
COMMENT                                   "@N" 1 11.1696 8.12273 0 -10 2.86386 0.733716 
COMMENT                                   #165#)) (CObjectList)) 
COMMENT                      #167=(CGroupWidget 
COMMENT                            (CWidget 22 (7 22 0) 1 2 0 0 Nil 28265 100) 
COMMENT                            (CObjectList 
COMMENT                             #168=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #10# 0) 1 2 0 0 #141# -401 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 13382451 0) 1 
COMMENT                                    0.117231 0.865776 1 0.0547715) 0.8 1.8 1) 
COMMENT                             #169=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #105# 481 100) 
COMMENT                                   (LOGPEN 0 0 4227264) 1 
COMMENT                                   (LOGFONT 29 10 0 0 400 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.16039 0.611111 0 "AmpR" "@N" 1 
COMMENT                                   -7.91576 10.4435 0 -10 1.53844 0.733716 #168#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #170=(CGroupWidget 
COMMENT                            (CWidget 25 (7 25 0) 1 2 0 0 Nil 4265 100) 
COMMENT                            (CObjectList 
COMMENT                             #171=(CLine 
COMMENT                                   (CWidget 0 (3 #4# 0) 1 2 0 0 #117# -251 100) 
COMMENT                                   (LOGPEN 0 6 32768) 8 0.890457 3.6446 3.8313) 
COMMENT                             #172=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #105# -96 100) 
COMMENT                                   (LOGPEN 0 0 4227264) 1 
COMMENT                                   (LOGFONT 29 10 0 0 400 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.16039 0.611111 0 "BGH polyA" "@N" 
COMMENT                                   1 8.94035 -10.4435 0 -10 2.62718 0.733716 
COMMENT                                   #171#) 
COMMENT                             #173=(CLine 
COMMENT                                   (CWidget 0 (3 #8# 0) 1 2 0 0 #117# 6450543 
COMMENT                                    100) (LOGPEN 0 6 32768) 8 0.890457 1.95038 
COMMENT                                   2.1588) 
COMMENT                             #174=(CLabel (CWidget 0 (0 0) 1 2 0 0 #105# 0 100) 
COMMENT                                   (LOGPEN 0 0 4227264) 1 
COMMENT                                   (LOGFONT 29 10 0 0 400 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.16039 0.611111 0 "SV40 polyA" 
COMMENT                                   "@N" 1 -12.625 -5.80195 0 -10 2.86386 
COMMENT                                   0.733716 #173#)) (CObjectList)) 
COMMENT                      #175=(CGroupWidget 
COMMENT                            (CWidget 27 (7 27 0) 1 2 0 0 Nil 16384200 100) 
COMMENT                            (CObjectList 
COMMENT                             #176=(CScratch 
COMMENT                                   (CWidget 0 (3 #12# 0) 1 2 0 0 #124# 
COMMENT                                    -1257566424 100) (LOGPEN 0 8 8388608) 10 1 
COMMENT                                   3.83303 1.9 0.0547715 1) 
COMMENT                             #177=(CScratch 
COMMENT                                   (CWidget 0 (3 #13# 0) 1 2 0 0 #124# 15 100) 
COMMENT                                   (LOGPEN 0 8 8388608) 10 1 5.52464 1.9 
COMMENT                                   0.0547715 1)) (CObjectList)) 
COMMENT                      #178=(CGroupWidget 
COMMENT                            (CWidget 29 (7 29 0) 1 2 0 0 Nil 395 100) 
COMMENT                            (CObjectList 
COMMENT                             #179=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #2# 0) 1 2 0 0 #142# 24848464 
COMMENT                                     100) (LOGPEN 0 6 32768) 8 
COMMENT                                    (LOGBRUSH 0 16777215 0) 0.890457 5.52377 
COMMENT                                    6.09256 1 0.0547715) 0.8 1.8 0) 
COMMENT                             #180=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #105# 211 100) 
COMMENT                                   (LOGPEN 0 0 4227264) 1 
COMMENT                                   (LOGFONT 29 10 0 0 400 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.16039 0.611111 0 "pCMV" "@N" 1 
COMMENT                                   7.93317 10.4435 0 -10 1.44376 0.733716 #179#) 
COMMENT                             #181=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #6# 0) 1 2 0 0 #142# 28265 100) 
COMMENT                                    (LOGPEN 0 6 32768) 8 (LOGBRUSH 0 16777215 0) 
COMMENT                                    1 2.89258 3.17567 1 0.0547715) 0.8 1.8 0) 
COMMENT                             #182=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #105# -255 100) 
COMMENT                                   (LOGPEN 0 0 4227264) 1 
COMMENT                                   (LOGFONT 29 10 0 0 400 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.16039 0.611111 0 "SV40" "@N" 1 
COMMENT                                   -2.82421 -12.7643 0 -10 1.37276 0.733716 
COMMENT                                   #181#)) (CObjectList)) 
COMMENT                      #183=(CGroupWidget 
COMMENT                            (CWidget 30 (7 30 0) 1 2 0 0 Nil 4865 100) 
COMMENT                            (CObjectList 
COMMENT                             #184=(CPromoterArrow 
COMMENT                                   (CLine 
COMMENT                                    (CWidget 0 (3 #3# 0) 1 2 0 0 #121# 1049 100) 
COMMENT                                    (LOGPEN 0 4 128) 6 1.10954 5.43737 5.52464) 
COMMENT                                   0.4 0.0547715 1.02954 1.8 1.8 0) 
COMMENT                             #185=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #105# 24872112 100) 
COMMENT                                   (LOGPEN 0 0 4227264) 1 
COMMENT                                   (LOGFONT 29 10 0 0 400 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.16039 0.611111 0 "T7" "@N" 1 
COMMENT                                   9.72321 9.28312 0 -10 0.710047 0.733716 #184#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #186=(CGroupWidget 
COMMENT                            (CWidget 33 (7 33 0) 1 2 0 0 Nil 16384280 100) 
COMMENT                            (CObjectList 
COMMENT                             #187=(CWideLine 
COMMENT                                   (CWidget 0 (3 #5# 0) 1 2 0 0 #145# 33554432 
COMMENT                                    100) (LOGPEN 0 6 6684927) 8 (LOGBRUSH 2 0 1) 
COMMENT                                   1 3.23125 3.59076 1 0.0547715) 
COMMENT                             #188=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #105# 314 100) 
COMMENT                                   (LOGPEN 0 0 4227264) 1 
COMMENT                                   (LOGFONT 29 10 0 0 400 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.16039 0.611111 0 "f1" "@N" 1 
COMMENT                                   5.06606 -11.6039 0 -10 0.497033 0.733716 
COMMENT                                   #187#) 
COMMENT                             #189=(CWideLine 
COMMENT                                   (CWidget 0 (3 #9# 0) 1 2 0 0 #145# 16384280 
COMMENT                                    100) (LOGPEN 0 6 6684927) 8 (LOGBRUSH 2 0 1) 
COMMENT                                   1 0.990823 1.57611 1 0.0547715) 
COMMENT                             #190=(CLabel (CWidget 0 (0 0) 1 2 0 0 #105# 18 100) 
COMMENT                                   (LOGPEN 0 0 4227264) 1 
COMMENT                                   (LOGFONT 29 10 0 0 400 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.16039 0.611111 0 "ColE1" "@N" 1 
COMMENT                                   -12.6789 3.48117 0 -10 1.53844 0.733716 #189#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #191=(CGroupWidget 
COMMENT                            (CWidget 86 (7 86 0) 1 2 0 0 Nil 17759 100) 
COMMENT                            (CObjectList 
COMMENT                             #192=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #7# 0) 1 2 0 0 #147# 1056 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 3368448 0) 1 
COMMENT                                    2.17182 2.86218 1 0.0547715) 0.8 1.8 0) 
COMMENT                             #193=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #105# 16420624 100) 
COMMENT                                   (LOGPEN 0 0 4227264) 1 
COMMENT                                   (LOGFONT 29 10 0 0 400 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.16039 0.611111 0 "NeoR" "@N" 1 
COMMENT                                   -8.53449 -10.4435 0 -10 1.37276 0.733716 
COMMENT                                   #192#)) (CObjectList))) (CObjectList)) 
COMMENT               #194=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil 1 100) 
COMMENT                     (CObjectList 
COMMENT                      #195=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #0# 0) 1 2 0 0 Nil 0 100) 
COMMENT                            (CObjectList 
COMMENT                             #196=(CScratch 
COMMENT                                   (CWidget 1 (1 #0# 1) 1 2 0 0 #122# 0 100) 
COMMENT                                   (LOGPEN 0 6 0) 8 1 3.8478 1.9 0.0547715 1) 
COMMENT                             #197=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #100# 4625 100) 
COMMENT                                   (LOGPEN 0 0 8421376) 1 
COMMENT                                   (LOGFONT 29 10 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.16039 0.611111 128 
COMMENT                                   "{\\i Apa}I (2794)" "@N (@S)" 5 10.2446 
COMMENT                                   -9.28312 0 -10 2.84019 0.662711 #196#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #198=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #1# 0) 1 2 0 0 Nil 57 100) 
COMMENT                            (CObjectList 
COMMENT                             #199=(CScratch 
COMMENT                                   (CWidget 1 (1 #1# 1) 1 2 0 0 #122# 9 100) 
COMMENT                                   (LOGPEN 0 6 0) 8 1 5.44822 1.9 0.0547715 1) 
COMMENT                             #200=(CLabel (CWidget 0 (0 0) 1 2 0 0 #100# 0 100) 
COMMENT                                   (LOGPEN 0 0 8421376) 1 
COMMENT                                   (LOGFONT 29 10 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.16039 0.611111 128 
COMMENT                                   "{\\i Kpn}I (951)" "@N (@S)" 5 11.9836 
COMMENT                                   6.96234 0 -10 2.55617 0.662711 #199#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #201=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil 73 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #202=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil 2082602612 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 0 
COMMENT       (CStringList) 1944749780 20592048 (CObList)))
FEATURES             Location/Qualifiers
     promoter        209..863
                     /vntifkey="29"
                     /label=pCMV
     promoter        863..882
                     /vntifkey="30"
                     /label=T7
     polyA_signal    2813..3027
                     /vntifkey="25"
                     /label=BGH\polyA
     rep_origin      3090..3503
                     /vntifkey="33"
                     /label=f1
     promoter        3568..3893
                     /vntifkey="29"
                     /label=SV40
     misc_recomb     3929..4723
                     /vntifkey="86"
                     /label=NeoR
     polyA_signal    4739..4978
                     /vntifkey="25"
                     /label=SV40\polyA
     rep_origin      5410..6083
                     /vntifkey="33"
                     /label=ColE1
     misc_marker     complement(6228..7089)
                     /vntifkey="22"
                     /label=AmpR
     misc_feature    920..946
                     /vntifkey="21"
                     /label=flag\epitope
     primer          complement(2811..2827)
                     /vntifkey="27"
                     /label=1503
     primer          863..882
                     /vntifkey="27"
                     /label=2506
     CDS             953..2788
                     /vntifkey="4"
                     /label=EIF4B
BASE COUNT     1801 a      1801 c      1889 g      1733 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 
      901 gtttaaactt aagcttacca tggattacaa ggacgatgac gataaggtac caatggcggc 
      961 ctcagcaaaa aagaagaata agaaggggaa gactatctcc ctaacagact ttctggctga 
     1021 ggatgggggt actggtggag gaagcaccta tgtttccaaa ccagtcagct gggctgatga 
     1081 aacggatgac ctggaaggag atgtttcgac cacttggcac agtaacgatg acgatgtgta 
     1141 tagggcgcct ccaattgacc gttccatcct tcccactgct ccacgggctg ctcgggaacc 
     1201 caatatcgac cggagccgtc ttcccaaatc gccaccctac actgcttttc taggaaacct 
     1261 accctatgat gttacagaag agtcaattaa ggaattcttt cgaggattaa atatcagtgc 
     1321 agtgcgttta ccacgtgaac ccagcaatcc agagaggttg aaaggttttg gttatgctga 
     1381 atttgaggac ctggattccc tgctcagtgc cctgagtctc aatgaagagt ctctaggtaa 
     1441 caggagaatt cgagtggacg ttgctgatca agcacaggat aaagacaggg atgatcgttc 
     1501 ttttggccgt gatagaaatc gggattctga caaaacagat acagactgga gggctcgtcc 
     1561 tgctacagac agctttgatg actacccacc tagaagaggt gatgatagct ttggagacaa 
     1621 gtatcgagat cgttatgatt cagaccggta tcgggatggg tatcgggatg ggtatcggga 
     1681 tggcccacgc cgggatatgg atcgatatgg tggccgggat cgctatgatg accgaggcag 
     1741 cagagactat gatagaggct atgattcccg gataggcagt ggcagaagag catttggcag 
     1801 tgggtatcgc agggatgatg actacagagg aggcggggac cgctatgaag accgatatga 
     1861 cagacgggat gatcggtcgt ggagctccag agatgattac tctcgggatg attataggcg 
     1921 tgatgataga ggtccccccc aaagacccaa actgaatcta aagcctcgga gtactcctaa 
     1981 ggaagatgat tcctctgcta gtacctccca gtccactcga gctgcttcta tctttggagg 
     2041 ggcaaagcct gttgacacag ctgctagaga aagagaagta gaagaacggc tacagaagga 
     2101 acaagagaag ttgcagcgtc agctggatga gccaaaacta gaacgacggc ctcgggagag 
     2161 acacccaagc tggcgaagtg aagaaactca ggaacgggaa cggtcgagga caggaagtga 
     2221 gtcatcacaa actgggacct ccaccacatc tagcagaaat gcacgaagga gagagagtga 
     2281 gaagtctcta gaaaatgaaa cactcaataa ggaggaagat tgccactctc caacttctaa 
     2341 acctcccaaa cctgatcagc ccctaaaggt aatgccagcc cctccaccaa aggagaatgc 
     2401 ttgggtgaag cgaagttcta accctcctgc tcgatctcag agctcagaca cagagcagca 
     2461 gtcccctaca agtggtgggg gaaaagtagc tccagctcaa ccatctgagg aaggaccagg 
     2521 aaggaaagat gaaaataaag tagatgggat gaatgcccca aaaggccaaa ctgggaactc 
     2581 tagccgtggt ccaggcgacg gagggaacag agaccactgg aaggagtcag ataggaaaga 
     2641 tggcaaaaag gatcaagact ccagatctgc acctgagcca aagaaacctg aggaaaatcc 
     2701 agcttccaag ttcagttctg caagcaagta tgctgctctc tctgttgatg gtgaagatga 
     2761 aaatgaggga gaagattatg ccgaataggg gcccgtttaa acccgctgat cagcctcgac 
     2821 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct 
     2881 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct 
     2941 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg 
     3001 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag 
     3061 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc 
     3121 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc 
     3181 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa 
     3241 tcggggcatc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact 
     3301 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt 
     3361 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa 
     3421 ccctatctcg gtctattctt ttgatttata agggattttg gggatttcgg cctattggtt 
     3481 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag 
     3541 ttagggtgtg gaaagtcccc aggctcccca ggcaggcaga agtatgcaaa gcatgcatct 
     3601 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 
     3661 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc 
     3721 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta 
     3781 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt 
     3841 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga 
     3901 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc 
     3961 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg 
     4021 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac 
     4081 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc 
     4141 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg 
     4201 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga 
     4261 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg 
     4321 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg 
     4381 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt 
     4441 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc 
     4501 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg 
     4561 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga 
     4621 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc 
     4681 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc 
     4741 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc 
     4801 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag 
     4861 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat 
     4921 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat 
     4981 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc 
     5041 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg 
     5101 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa 
     5161 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac 
     5221 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt 
     5281 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 
     5341 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 
     5401 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 
     5461 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 
     5521 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 
     5581 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 
     5641 tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc 
     5701 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 
     5761 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 
     5821 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 
     5881 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 
     5941 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 
     6001 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 
     6061 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 
     6121 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 
     6181 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 
     6241 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 
     6301 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 
     6361 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 
     6421 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 
     6481 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 
     6541 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 
     6601 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 
     6661 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 
     6721 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 
     6781 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 
     6841 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 
     6901 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 
     6961 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 
     7021 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 
     7081 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 
     7141 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 
     7201 ccccgaaaag tgccacctga cgtc 
//
