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GeneCorner host details

Last data update: 23 September 2021 14:51 CEST

Host name: G4 ROSALUC mouse ES cells (LMBP 10507CB)

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Source:Mouse
Type:Embryonic stem (ES) cells
Cell line designation:G4 ROSALUC B12
Other designation:G4 ROSALUC ES
Accession number: LMBP 10507CB
Depositor: Dr T. Hochepied1 2, Dr L. Haenebalcke1 2 and Prof. Dr J. Haigh1 2
1 Inflammation Research Center, VIB, Ghent, Belgium
2 Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Containment level: This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification).
Medium: • 500 ml bottle Knockout DMEM (Invitrogen, cat. no. 10829-018)
• 90 ml serum
• 6 ml penicillin/streptomycin 100X
• 6 ml beta-mercaptoethanol 100X
• 6 ml non-essential amino acids 100X
• 6 ml glutamine 100X
• recombinant mouse LIF 2000 units/ml
Selection marker: puromycin (1 µg/ml)
Temperature: 37°C
Cultivation remarks:Recovery: startup in a 6-well with inactivated feeders after washing.
Genotype: -
Phenotype: -
Properties: -
Further information: -
Original reference: Haenebalcke et al., Stem Cell Rev. 6 (2013), 774-785 [PMID: 23877658]
Restricted use: - VIB/MSH/BCCM MTA
- Commercial, for-profit entities require a use license from VIB / MT SINAI prior to the release of the material.
Cultivation remarks:Recovery: startup in a 6-well with inactivated feeders after washing.

Refer in your Materials and Methods:

G4 ROSALUC mouse ES cells (LMBP 10507CB) is available at BCCM/GeneCorner. It was deposited by Dr T. Hochepied, Dr L. Haenebalcke and Prof. Dr J. Haigh and was published in Haenebalcke et al., 2013.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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