GeneCorner host details

Last data update: 23 September 2021 14:51 CEST

Host name: Candida albicans Tet-On-RT2 (LMBP 10529)

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Species:Candida albicans
Strain designation:Tet-On-RT2
Accession number: LMBP 10529
Depositor: Prof. Dr K. Ganesan1. It was constructed by Dr S. Bijlani1.
1 CSIR Institute of Microbial Technology, Chandigarh, India
Containment level: This strain has been assigned the containment level Class 2 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification).
Medium: YPD (1% bacto-yeast extract, 2% bacto-peptone and 2% dextrose)
Selection marker: nourseothricine (200 µg/ml)
Temperature: 30°C
Genotype: HAH2-pTET-TUP1/HIS1-pTET-TUP1
Phenotype: NtcR
Properties: The strain contains the C. albicans TUP1 gene under the control of a TET promoter, and a Tet-On transactivator under the control of the C. albicans RPS1 promoter, integrated at the RPS1 locus.
Further information: The strain is a derivative of the C. albicans Tet-On-R strain (LMBP 10527) which is a derivative of C. albicans SN95.
Original reference: Bijlani et al., Curr. Genet. 64 (2018), 303-316 [PMID: 28597304] [DOI: 10.1007/s00294-017-0720-9]
Restricted use: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded namely UGent-DBMB and VIB-IRC.

Refer in your Materials and Methods:

Candida albicans Tet-On-RT2 (LMBP 10529) is available at BCCM/GeneCorner. It was deposited by Prof. Dr K. Ganesan and was published in Bijlani et al., 2018.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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