Last data update: 20 September 2021 09:16 CEST
Host name: Escherichia coli K12 MC1061(λ) (LMBP 1061)
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|Accession number:||LMBP 1061|
|Depositor:||Prof. Dr E. Remaut1 2
1 Department for Molecular Biomedical Research, VIB, Ghent, Belgium
2 Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Containment level:||This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification).|
|Temperature:||28°C (Note: 37°C is also possible but phage lambda remains more stably integrated when grown at 28°C)|
|Genotype:||F- Δ(araA-leu)7697 [araD139]B/r Δ(codB-lacI)3 galK16 galE15(GalS) e14- mcrA0 relA1 rpsL150 spoT1 mcrB1 hsdR2 λ+|
|Phenotype:||StrR rK- mK+|
|Properties:||Useful host for primary transformation. Transforms very well by the CaCl2 method (10exp7/μg). There is no Type I restriction; incoming DNA receives the E. coli K modification. Lysogenized with λ wild type. Recommended as maintenance host for plasmids containing the PL or PR promoter from phage λ. It is recommended to grow MC1061(λ), containing pPL plasmids, at 28°C.|
|Further information:||As this strain is deleted for the lacI repressor gene, it is not a suitable host for plasmids carrying the lac promoter or derivatives thereof, such as tac, trc, N25/O2 ... promoters. In the absence of repression, continuous transcription from the lac promoter is likely to result in plasmid instability.
Constructed by lysogenizing λ in MC1061 (Casadaban et al., 1980).
Contains no T7RNAP and the λ wt repressor efficiently represses the λ PL promoter.
|Original reference:||Mertens et al., Gene 164 (1995), 9-15 [PMID: 7590329]
|Related reference:||Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
|Restricted use:||- BCCM MTA|
Refer in your Materials and Methods:
|Escherichia coli K12 MC1061(λ) (LMBP 1061) is available at BCCM/GeneCorner. It was deposited by Prof. Dr E. Remaut and was published in Mertens et al., 1995.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.