Last data update: 24 January 2024 16:39 CET
Host name: Escherichia coli FHK12 (LMBP 11202)
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| Species: | Escherichia coli |
| Group: | K12 |
| Strain designation: | FHK12 |
| Accession number: | LMBP 11202 |
| Depositor: | Dr A. Fulara1 2 and Prof. Dr S. Savvides1 2 1 Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium 2 VIB-UGent Center for Inflammation Research, VIB, Ghent, Belgium It was received from Prof Dr D. Langosch3 3 Center for Integrated Protein Science Munich CIPSM, Technical University of Munich, Germany |
| Containment level: | This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification). |
| Medium: | LB-Lennox |
| Selection marker: | ampicillin (100 μg/ml) |
| Temperature: | 37°C |
| Genotype: | F' lacIq lacZΔM15 proA+B+ ara Δ(lac-proAB) rpsL (ϕ80dlacZΔM15) attB::(ctx::lacZ) (source: Kolmar et al., 1995) |
| Phenotype: | ampR strR |
| Properties: | The ctx::lacZ fusion integrated at the attB site is tagged with the ampicillin resistance gene. |
| Further information: | This FHK12 strain was constructed as follows: First FHK11 was constructed from strain JM83 (Yanish-Perron et al., 1985) by chromosomal integration of the lacZ gene under control of the V. cholerae ctxAB promoter from plasmid pLDR10-ctx::lacZ at the phage λ attB site (Diederich et al., 1992). FHK12 was subsequently constructed from FHK11 by F-duction (Miller, 1972) with BMH71-18 (source B.Müller-Hill). The streptomycin resistance has been experimentally confirmed by BCCM/GeneCorner. |
| Original reference: | Kolmar et al., EMBO J. 14 (1995), 3895-3904 [PMID: 7664730] |
| Related reference: | Kolmar et al., Biol. Chem. Hoppe-Seyler 375 (1994), 61-70 [PMID: 8003258] [DOI: 10.1515/bchm3.1994.375.1.61] |
| Restricted use: | - BCCM MTA |
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Refer in your Materials and Methods: |
Escherichia coli FHK12 (LMBP 11202) is available at BCCM/GeneCorner. It was deposited by Dr A. Fulara and Prof. Dr S. Savvides and was published in Kolmar et al., 1995. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.