GeneCorner host details

Last data update: 24 January 2024 16:39 CET

Host name: Escherichia coli FHK12 (LMBP 11202)

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Species:Escherichia coli
Strain designation:FHK12
Accession number: LMBP 11202
Depositor: Dr A. Fulara1 2 and Prof. Dr S. Savvides1 2
1 Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium
2 VIB-UGent Center for Inflammation Research, VIB, Ghent, Belgium
It was received from Prof Dr D. Langosch3
3 Center for Integrated Protein Science Munich CIPSM, Technical University of Munich, Germany
Containment level: This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification).
Medium: LB-Lennox
Selection marker: ampicillin (100 μg/ml)
Temperature: 37°C
Genotype: F' lacIq lacZΔM15 proA+B+ ara Δ(lac-proAB) rpsL (ϕ80dlacZΔM15) attB::(ctx::lacZ) (source: Kolmar et al., 1995)
Phenotype: ampR strR
Properties: The ctx::lacZ fusion integrated at the attB site is tagged with the ampicillin resistance gene.
Further information: This FHK12 strain was constructed as follows:

First FHK11 was constructed from strain JM83 (Yanish-Perron et al., 1985) by chromosomal integration of the lacZ gene under control of the V. cholerae ctxAB promoter from plasmid pLDR10-ctx::lacZ at the phage λ attB site (Diederich et al., 1992).

FHK12 was subsequently constructed from FHK11 by F-duction (Miller, 1972) with BMH71-18 (source B.Müller-Hill).

The streptomycin resistance has been experimentally confirmed by BCCM/GeneCorner.
Original reference: Kolmar et al., EMBO J. 14 (1995), 3895-3904 [PMID: 7664730]
Related reference: Kolmar et al., Biol. Chem. Hoppe-Seyler 375 (1994), 61-70 [PMID: 8003258] [DOI: 10.1515/bchm3.1994.375.1.61]
Restricted use: - BCCM MTA

Refer in your Materials and Methods:

Escherichia coli FHK12 (LMBP 11202) is available at BCCM/GeneCorner. It was deposited by Dr A. Fulara and Prof. Dr S. Savvides and was published in Kolmar et al., 1995.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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