GeneCorner host details

Last data update: 24 January 2024 16:39 CET

Host name: Escherichia coli K12 JM109 (LMBP 1126)

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Species:Escherichia coli
Strain designation:JM109
Accession number: LMBP 1126
Depositor: This host strain was deposited by Prof. Dr R. Beyaert1 2. It was obtained from Dr J. Messing 3.
1 VIB-UGent Center for Inflammation Research, VIB, Ghent, Belgium
2 Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
3 Waksman Institute, Rutgers, State University of New Jersey, Piscataway, USA
Containment level: This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification).
Other culture collection numbers:DSM 3423
ATCC 53323
NCIMB 12711
Medium: LB-Lennox
Selection marker: Nalidixic acid
Temperature: 37°C
Genotype: [F´ traD36 proAB laqIqZΔM15] λ- endA1 recA1 gyrA96 thi hsdR17 relA1 supE44 Δ(lac-proAB)
Phenotype: NalR rk- mk+
Properties: Bacterial Strain JM109 is a useful host for transformation of pGEM Vectors and for production of single-stranded DNA from M13 or phagemid vectors. The strain grows well and is transformed efficiently by a variety of methods. Because JM109 is recA- and lacks the E. coli K restriction system, there is no undesirable restriction of cloned DNA or recombination with host chromosomal DNA. The endonuclease A- mutation leads to an improved yield and quality of isolated plasmid DNA. JM109 is deficient in β-galactosidase activity due to deletions in both genomic and episomal copies of the lacZ gene. The deletion in the episomal (F´ factor) copy of the lacZ gene (lacZΔM15) can be complemented by addition of a functional α-peptide encoded by a pGEM-Z or pGEM-Zf Vector. If complementation does not occur, bacterial colonies are white. To maintain the F´, JM109 should be grown on minimal (M-9) media supplemented with 1mM thiamine.
Further information: There is a genome sequence of the JM109 strain available at Genbank (Accession number: NZ_RYWX00000000.1).

The nalidixic acid resistance has been experimentally verified by BCCM/GeneCorner.
Original reference: Yanisch-Perron et al., Gene 33 (1985), 103-119 [PMID: 2985470]
Restricted use: - BCCM MTA

Refer in your Materials and Methods:

Escherichia coli K12 JM109 (LMBP 1126) is available at BCCM/GeneCorner. It was deposited by Prof. Dr R. Beyaert and was published in Yanisch-Perron et al., 1985.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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