GeneCorner host details

Last data update: 24 January 2024 16:39 CET

Host name: Escherichia coli K12 WG1260 (LMBP 12432)

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Species:Escherichia coli
Strain designation:WG1260
Accession number: LMBP 12432
Depositor: This host strain was deposited by Dr D.E. Culham1 and Prof. Em. Dr J.M. Wood1.
1 Department of Molecular and Cellular Biology, University of Guelph, Guelph, Canada
Containment level: This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification).
Medium: LB-Lennox
Selection marker: -
Temperature: 37°C
Genotype: F- trp lacZ rpsL thi ∆putPA101 ∆(proU)600 ∆(proP-melAB)212 clsA::Tn10dTet3 proP678::Lumio
Phenotype: StrR TetR
Properties: -
Further information: The allele cls::Tn10dTet3 was introduced into WG1259 by P1 transduction. For the construction of WG1259 the proP678::Lumio ORF was introduced into the chromosome of WG1224 by allelic exchange as described by Link et al. (1997).

Resistance to streptomycin and tetracycline has been experimentally confirmed by BCCM/GeneCorner.
Original reference: Romantsov et al., J. Bacteriol. 192 (2010), 912-924 [PMID: 20008071] [DOI: 10.1128/JB.00967-09]
Related reference: Link et al., J. Bacteriol. 179 (1997), 6228-6237 [PMID: 9335267] [DOI: 10.1128/jb.179.20.6228-6237.1997]
Restricted use: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department.

Refer in your Materials and Methods:

Escherichia coli K12 WG1260 (LMBP 12432) is available at BCCM/GeneCorner. It was deposited by Dr D.E. Culham and Prof. Em. Dr J.M. Wood and was published in Romantsov et al., 2010.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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