GeneCorner host details

Last data update: 24 January 2024 16:39 CET

Host name: Escherichia coli K12 WG914 (LMBP 12434)

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Species:Escherichia coli
Strain designation:WG914
Accession number: LMBP 12434
Depositor: This host strain was deposited by Dr D.E. Culham1 and Prof. Em. Dr J.M. Wood1.
1 Department of Molecular and Cellular Biology, University of Guelph, Guelph, Canada
Containment level: This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification).
Medium: LB-Lennox
Selection marker: -
Temperature: 37°C
Genotype: F- trp lacZ rpsL thi ∆putPA101 proU205 ∆proQ676
Phenotype: StrR
Properties: -
Further information: This host strain was created by inserting an in-frame chromosomal proQ deletion (∆proQ676) into E. coli K12 WG210.

Resistance to streptomycin has been experimentally confirmed by BCCM/GeneCorner.
Original reference: Smith et al., Biochemistry 43 (2004), 12979-12989 [PMID: 15476391] [DOI: 10.1021/bi048561g]
Restricted use: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department.

Refer in your Materials and Methods:

Escherichia coli K12 WG914 (LMBP 12434) is available at BCCM/GeneCorner. It was deposited by Dr D.E. Culham and Prof. Em. Dr J.M. Wood and was published in Smith et al., 2004.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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