GeneCorner host details

Last data update: 24 January 2024 16:39 CET

Host name: Escherichia coli K12 WG1289 (LMBP 12438)

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Species:Escherichia coli
Strain designation:WG1289
Accession number: LMBP 12438
Depositor: This host strain was deposited by Dr D.E. Culham1 and Prof. Em. Dr J.M. Wood1.
1 Department of Molecular and Cellular Biology, University of Guelph, Guelph, Canada
Containment level: This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification).
Medium: LB-Lennox
Selection marker: -
Temperature: 37°C
Genotype: ΔproP837::FRT ΔproW859::FRT ΔbetT856::FRT ΔotsA847::FRT ΔyehW854::FRT
Phenotype: -
Properties: -
Further information: Gene yehW was deleted from E. coli WG1246 as described by Datsenko and Wanner (2000). The Keio collection isolate JW2116-2 served as donor of ΔyehW754::kan yielding strain WG1289 (ΔyehW854::FRT).

WG1289 as a WG1246 derivative cannot synthesize trehalose (ΔotsA) or take up osmoprotectants via transporters BetT, ProP or ProU. It lacks the integral membrane subunit ProW of the ProU system.

WG1246 is a derivative of MG1655.
Original reference: Lang et al., Biochemistry 54 (2015), 5735-5747 [PMID: 26325238] [DOI: 10.1021/acs.biochem.5b00274]
Related reference: Murdock et al., Appl. Environ. Microbiol. 80 (2014), 5366−5378 [PMID: 24951793] [DOI: 10.1128/AEM.01138-14]
Datsenko and Wanner, Proc. Natl. Acad. Sci. U.S.A. 97 (2000), 6640−6645 [PMID: 10829079] [DOI: 10.1073/pnas.120163297]
Restricted use: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department.

Refer in your Materials and Methods:

Escherichia coli K12 WG1289 (LMBP 12438) is available at BCCM/GeneCorner. It was deposited by Dr D.E. Culham and Prof. Em. Dr J.M. Wood and was published in Lang et al., 2015.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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