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GeneCorner host details

Last data update: 24 January 2024 16:39 CET

Host name: Escherichia coli K12 WG389 (LMBP 12441)

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Species:Escherichia coli
Group:K12
Strain designation:WG389
Accession number: LMBP 12441
Depositor: This host strain was deposited by Dr D.E. Culham1 and Prof. Em. Dr J.M. Wood1.
1 Department of Molecular and Cellular Biology, University of Guelph, Guelph, Canada
Containment level: This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification).
Medium: LB-Lennox + pro (25 mM)
Selection marker: -
Temperature: 37°C
Genotype: F- trp lacZ rpsL thi ∆putPA101 ∆(proU)600 ∆(proP-melAB)212 ∆(brnQ phoA proC)
Phenotype: StrR
Properties: This is a proline auxotroph lacking all known proline uptake systems.
Further information: Resistance to streptomycin has been experimentally confirmed by BCCM/GeneCorner.
Original reference: Culham et al., J. Mol. Biol. 229 (1993), 268-276 [PMID: 8421314] [DOI: 10.1006/jmbi.1993.1030]
Restricted use: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department.

Refer in your Materials and Methods:

Escherichia coli K12 WG389 (LMBP 12441) is available at BCCM/GeneCorner. It was deposited by Dr D.E. Culham and Prof. Em. Dr J.M. Wood and was published in Culham et al., 1993.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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