GeneCorner host details

Last data update: 24 January 2024 16:39 CET

Host name: Escherichia coli K12 WG980 (LMBP 12443)

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Species:Escherichia coli
Strain designation:WG980
Accession number: LMBP 12443
Depositor: This host strain was deposited by Dr D.E. Culham1 and Prof. Em. Dr J.M. Wood1.
1 Department of Molecular and Cellular Biology, University of Guelph, Guelph, Canada
Containment level: This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification).
Medium: LB-Lennox
Selection marker: -
Temperature: 37°C
Genotype: F- trp lacZ rpsL thi ∆putPA101 ∆(proU)600 ∆(proP-melAB)212 clsA::Tn10dTet3
Phenotype: TetR StrR
Properties: -
Further information: WG980 was isolated as a tetracycline-resistant P1 cml clr100 transductant of WG350 from WC3899 that harbours the cls::Tn10dTet3 insertion.

Resistance to streptomycin and tetracycline has been experimentally confirmed by BCCM/GeneCorner.
Original reference: Romantsov et al., Mol. Microbiol. 64 (2007), 1455-1465 [PMID: 17504273] [DOI: 10.1111/j.1365-2958.2007.05727.x]
Restricted use: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department.

Refer in your Materials and Methods:

Escherichia coli K12 WG980 (LMBP 12443) is available at BCCM/GeneCorner. It was deposited by Dr D.E. Culham and Prof. Em. Dr J.M. Wood and was published in Romantsov et al., 2007.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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