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GeneCorner host details

Last data update: 24 October 2021 17:14 CEST

Host name: Escherichia coli K12 MG1655 SEGA012 (LMBP 12848)

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Species:Escherichia coli
Group:K12
Strain designation:MG1655 SEGA012
Accession number: LMBP 12848
Depositor: A. Ehrmann1 and Prof. Dr M. Nørholm1. It was constructed by C. Bayer1, A. Ehrmann1 and M. Rennig1.
1 The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Horsholm, Denmark
Containment level: This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification).
Other culture collection numbers:SEGA 012
Medium: LB-Lennox
Selection marker: tetracycline (10 µg/ml)
Temperature: 37°C
Genotype: F- λ- ilvG- rfb-50 rph-1 glmS-pstS::J23100-medium-TEV-gfp-tetA (Source: http://openwetware.org/wiki/E._coli_genotypes#MG1655 + Bayer et al., 2021)
SEGA sequence:p12848.gb
Phenotype: tetR
Properties: The strain was created based on Escherichia coli K12 MG1655, by integrating the SEGA landing pad between the glmS and pstS loci. Counter-selection is posssible through sensitivity to NiCl2 (50 µM).
Further information: This host strain is intended for simplistic bacterial genome engineering, using the SEGA genome architecture.
Original reference: Bayer et al., Nat. Commun. 12 (2021), 5876 [DOI: 10.1038/s41467-021-26155-5]
Restricted use: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department.

Refer in your Materials and Methods:

Escherichia coli K12 MG1655 SEGA012 (LMBP 12848) is available at BCCM/GeneCorner. It was deposited by A. Ehrmann and Prof. Dr M. Nørholm and was published in Bayer et al., 2021.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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