Last data update: 22 May 2023 13:52 CEST
Host name: Escherichia coli K12 MG1655 SEGA014 (LMBP 12850)
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|Strain designation:||MG1655 SEGA014|
|Accession number:||LMBP 12850|
|Depositor:||A. Ehrmann1 and Prof. Dr M. Nørholm1. It was constructed by C. Bayer1, A. Ehrmann1 and M. Rennig1.
1 The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Horsholm, Denmark
|Containment level:||This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification).|
|Other culture collection numbers:||SEGA 014|
|Selection marker:||tetracycline (10 µg/ml)|
|Genotype:||F- λ- ilvG- rfb-50 rph-1 glmS-pstS::J23100-low-PROTi-gfp-tetA (Source: http://openwetware.org/wiki/E._coli_genotypes#MG1655 + Bayer et al., 2021)|
|Properties:||The strain was created based on Escherichia coli K12 MG1655, by integrating the SEGA landing pad between the glmS and pstS loci. Counter-selection is posssible through sensitivity to NiCl2 (50 µM).|
|Further information:||This host strain is intended for simplistic bacterial genome engineering, using the SEGA genome architecture.
More info can be found on https://sega-genomes.com.
|Original reference:||Bayer et al., Nat. Commun. 12 (2021), 5876 [PMID: 34620865] [DOI: 10.1038/s41467-021-26155-5]
|Restricted use:||- BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department.
Refer in your Materials and Methods:
|Escherichia coli K12 MG1655 SEGA014 (LMBP 12850) is available at BCCM/GeneCorner. It was deposited by A. Ehrmann and Prof. Dr M. Nørholm and was published in Bayer et al., 2021.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.